Lipid Peroxidation In Rats Research Articles

Dose-Dependent Molecular Responses of Labeo rohita to Triphenyl Phosphate.

Triphenyl phosphate (TPhP) is a broad-spectrum organophosphate compound widely used as an additive in several products to prevent ignition. However, its utilization produces a hazardous impact on various organisms. So far, very few studies have investigated the acute toxicity of TPhP at environmentally relevant concentrations in nontarget aquatic species. This study aimed to assess whether the short-term exposure of TPhP (4, 20, and 100 μg L-1) affects the oxidative stress, antioxidant activity, biomolecule metabolism, DNA stability, chromosomal integrity, apoptosis, and pathological changes in various organs of Labeo rohita fingerlings. The results illustrated that the reactive oxygen species (ROS) production and lipid peroxidation (LPO) rates were significantly higher in tissues (brain, liver, and kidney) of TPhP-treated groups. Interestingly, superoxide dismutase (SOD) and catalase (CAT) activities were remarkably decreased in tissues following TPhP exposure. The levels of protein, glucose, total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) in various tissues were also found to be significantly altered in TPhP-exposed fish fingerlings. These significant alterations in the antioxidant system and biochemical profile induced genotoxic responses such as DNA and chromosomal damage in the fish fingerlings. Furthermore, the incidence of the observed genotoxic responses was also found to be dose-dependent. Likewise, the apoptotic responses were also significantly altered following TPhP acute exposure in L. rohita fingerlings. The subsequent effects on oxidative stress, antioxidant inhibition, dysregulated biomolecule metabolism, and genotoxicity might be the possible reason for the observed pathological changes in various tissues of L. rohita. Taken together, the present findings showed that the toxicity of TPhP is principally associated with exposure concentrations. Therefore, this study illustrates the toxicity risks of TPhP to vertebrate organisms at real-world concentrations.

Detection of lipid peroxidation in dog spermatozoa with the fluorescent dye C11-BODIPY581/591.

C11-BODIPY581/591 is a fluorescent probe that has been successfully used to evaluate lipid peroxidation in different species, but it has not been completely studied in the dog. Thus, the aim of the present study was to assess lipid peroxidation of dog spermatozoa using C11-BODIPY581/591 and compare different positive controls of the technique. Twenty-four ejaculates were collected from 8 adult male dogs. Routine seminal characteristics were evaluated in raw semen. Lipid peroxidation evaluation was performed as described in other species. Samples were divided in three aliquots, exposed to UV radiation, incubated with hydrogen peroxide or left without treatment (control). Lipid peroxidation was significantly greater only in UV-exposed samples than in the control ones (91±6% vs. 8.3±3.5%, p˂.01). In conclusion, C11-BODIPY581/591 is useful to evaluate lipid peroxidation of dog spermatozoa and UV radiation is a good promoter of membrane oxidation, so irradiated samples can be used as a positive control of this technique.

Soybean roots defence against cadmium and its dependence on dose in a non-linear manner

Abstract Metal pollution represents a serious issue for sustainable agronomy and food safety. Activation of plants’ protective mechanisms has been shown to depend on the extent of soil contamination with metal, but reports on dose-dependent responses (especially to extreme concentrations) are rather rare. In this research we exposed soybean (Glycine max L.) roots at a very early stage to a scale of cadmium concentrations, including doses far exceeding the amounts found in nature. Spectrophotometrical and enzyme in-gel detection assays were used to examine ongoing defence responses. The results confirmed commonly reported findings on inhibited growth and the activation of several superoxide dismutase isoforms in a dose-dependent manner. Contrary to expectations, the hydrogen peroxide levels, proline accumulation and the rate of lipid peroxidation were suppressed with increasing metal doses. More importantly, the linearity of Cd accumulation in soybean roots was interrupted at 200 mg.l-1 of cadmium, which coincides with peculiar responses of several chitinase family members. No such nonlinear response was observable for proline accumulation or any of the SOD isoforms. Possible explanations are provided and the importance of considering the metal stress dose is stressed to avoid false generalizations on plant defence responses.

The protective effect of Vitamin D and combination with liv-52 on lipid profiles in carbon tetrachloride-induced liver disease in Wistar rats

Background: Liver is the largest gland, accounts for approximately 2.5% of total body weight and also liver called as the metabolic “engine-room of the body.” Liver plays role in both metabolism as well as biochemical transformation. Therefore, it is vital to maintain a healthy liver for overall health and well-being. However, liver is continuously exposed to exogenous substances such as toxins, drugs, and alcohol which can ultimately lead to various liver disorders. Liver diseases today are one of the most fatal diseases globally. Aims and Objectives: The aim of the present study was to evaluate lipid peroxidation (LPO) and lipid profile levels of Vitamin D and combination with Liv-52 on carbon tetrachloride induced liver disease in rats. Materials and Methods: Thirty-six adult male Albino Wistar rats weighing 150–200 g were used in this study. Liver disease was induced in rats by administration of Carbon tetrachloride (CCl4) intraperitoneally at the dose of 1 mL mixed with 50% of olive oil twice a week for 5 weeks, 3 days interval between each dose, after confirmation of liver disease treated with Vitamin D and Liv-52 for 5 weeks. Results: The levels of LPO were significantly decreased in Vitamin D and Liv-52 treated animals when compared with CCl4 induced animals. Total cholesterol, free cholesterol, phospholipids, and triglycerides (TGs) levels were significantly reduced when compared with CCl4 induced rats. However, the levels of ester cholesterol and free fatty acids (FFAs) were significantly increased in Vitamin D and Liv-52 treated animals when compared with CCl4 induced animals. Conclusion: Vitamin D and Liv-52 effectively reduced the LPO levels. Treatment with Vitamin D and Liv-52, the levels of total and free cholesterol, phospholipids, and TGs were significantly reduced and treatment with both combinations highly reduced all these levels when compared with CCl4 induced animals. And treatment with Vitamin D and Liv-52, the ester cholesterol and FFAs levels were increased.

Study of effect of Astragalus vulpinus extract on lipid peroxidation processes in hypothalamic region on lipopolysaccharide immune stress model

An experimental study is devoted to the study of the effect of Astragalus vulpinus extract on lipid peroxidation processes in the hypothalamic region under conditions of lipopolysaccharide immune stress. Material and methods. Experiments were performed on white male rats 6-8 months of age. In all series of experiments, animals were divided into groups (n = 10): 1st – control individuals receiving distilled water in an equivalent volume; 2nd – rats with immune stress model; 3rd – individuals treated with Astragalus vulpinus extract against the background of immune stress. The test extract and distilled water were administered once a day intragastrically with a 50 mg/kg probe for 14 days. Immune stress was formed by single intraperitoneal administration of lipopolysaccharide pyrogenal isolated from Salmonella typhi microbial cells. The intensity of free radical oxidation processes was studied by thiobarbituric acid reactive substances (TBARS) content, spontaneous and ascorbate-dependent lipid peroxidation rate and catalase activity were also determined. Results. According to the results of the experiment, it was revealed that immune stress is accompanied by the activation of free-radical oxidation processes, as evidenced by an increase in the concentration of products of this process and the activity of the body’s antioxidant protection enzyme catalase. Assessment of the effect of Astragalus vulpinus herb extract on the free radical potential in the hypothalamic region against the background of the action of lipopolysaccharide-induced immune stress showed that the studied extract exhibits pronounced stress-protective and antioxidant properties characterized by changing the intensity of lipid peroxidation processes and by increase of catalase activity in the tissue of the hypothalamic brain region. Conclusions. Astragalus vulpinus herb extract has been proven to exhibit antioxidant effects by restoring activity of free radical processes.

Evaluating the therapeutic potential of white button mushroom (Agaricus bisporus) against DMBA-induced breast cancer in Sprague Dawley rats.

The current research work was designed to investigate the protective effects of white button mushroom (Agaricus bisporus) against 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer. Breast cancer was induced in rats by the administration of a single dose of 50mg/kg DMBA via gavage. The rats were divided into four groups: G1 (negative control group), G2 (positive control group), G3 (rats receiving mushroom extract), and G4 (rats administered with doxorubicin). The mushroom extract significantly (p<.001) improved the activity of antioxidant enzymes in carcinogenic rats. Moreover, the mushroom extract also prevented the increase in the concentration of tumor biomarkers that are CEA, CA 15.3, and CRP in experimental rats. Liver function enzymes were also raised in G2 and G4 compared with G3. Besides, the RBCs and Hb were also reduced significantly in G4 while in G3. The mushroom extract effectively controlled the level of RBCs and Hb. An improvement in lipid profile was also measured in mushroom extract receiving rats. Conclusively, the mushroom extract alleviated DMBA-induced breast cancer potentially via improving antioxidants, reducing lipid peroxidation, and decreasing tumor biomarkers. PRACTICAL APPLICATIONS: The present research study examined the antitumor potential of white button mushroom. The mushroom effectively prevented the increase in tumor biomarkers, reduction in antioxidant enzymes, and increase in lipid peroxidation in rats with DMBA-induced breast cancer. The mushroom can be used as a potential source to prevent breast cancer and further research can be conducted to explore its anticancer mechanisms.

Silicon Nanoparticles Mitigate Hypoxia-Induced Oxidative Damage by Improving Antioxidants Activities and Concentration of Osmolytes in Southern Highbush Blueberry Plants

Climate change exacerbates flooding problems due to hurricanes followed by heavy rains, particularly in sub-tropical regions. Consequently, submerged plants experience hypoxia stress which limits agronomic and horticultural crop growth and production. Hypoxia causes oxidative damage by accelerating the lipid peroxidation associated with O2- and H2O2 levels. Additionally, hypoxia increases the accumulation of organic osmoprotectants and antioxidant activity, whereas it decreases the macronutrient (N, P, K, and Zn) uptake. This study aimed at investigating the effects of flooding-induced hypoxia stress on the growth and the physiological, biochemical, and nutritional characteristics of the hydroponically grown southern highbush blueberry (cv. Jewel) plants. In addition, the hypoxia-mitigating effects of conventional silicon (Si-C) and silicon nanoparticles (SiNPs) and their application methods (foliar vs. foliar and rootzone application) were also appraised. Both the Si-C and the SiNPs efficiently alleviated hypoxia-induced oxidative and osmotic damage to cells by enhancing the activities of the enzymatic antioxidants (ascorbate peroxidase, catalase, dehydroascorbate reductase, superoxide dismutase, peroxidase, guaiacol peroxidase, monodehydroascorbate, reductase); the non-enzymatic antioxidants (ascorbic acid and glutathione contents); and the accumulation of compatible solutes (proline and glycinebetaine) in leaves and roots. However, the SiNPs were more effective than Si-C at improving antioxidant activities and osmolytes formation. A strong negative correlation between the antioxidant activities and the lipid peroxidation rate was observed in the SiNP-treated plants under hypoxia stress. The SiNPs also regulated nutrient uptake by increasing the K, N, P, and Zn concentrations while decreasing Fe and Mn concentrations to a less toxic level. Blueberry plants treated with SiNPs responded more effectively to hypoxia stress by maintaining higher antioxidant and osmoprotectant concentrations than blueberry plants treated with Si-C. Additionally, the foliar and rootzone applications yielded better results than the foliar applications only.

Evaluation of Complete Fertilizer in the Aspect of the Antioxidant Enzyme System of Maize Hybrids

Studies on physiological and biochemical processes in crops are highly relevant for breeders to produce hybrids with high yield. Two different maturity groups of maize hybrids were tested in this study. The research site was located at the Látókép Experimental Station of the University of Debrecen and the experiment lasted for 2 years. The examined nitrogen ranges were separated into two parts. Firstly, the effects of nitrogen fertilizer ranging from 120–300 kg ha−1 were examined, supplemented with a constant, high-level P2O5 and K2O. Secondly, the optimal ratio of N:P:K was measured. In order to monitor the health status of maize hybrids, stress indicators including the activity of ascorbate peroxidase (APX), and superoxide dismutase (SOD), the rate of lipid peroxidation (LP), and grain yield were measured. The samples were taken in five phenological stages. Variance analysis based on nitrogen fertilizer showed variation in sampling times and fertilizers on APX, LP, and SOD. Variance analysis based on NPK indicated variation in sampling times, years, and fertilizer levels on APX, LP, and SOD. Correlation analysis showed that yield correlated negatively with SOD during the use of NPK fertilizer, as the use of nitrogen fertilizer cannot make corrections to yield with SOD but phosphorus and potassium can correlate with yield, and SOD. Principal component analysis showed that NPK5 and N5 had maximum stability and effect on yield. The activity of APX had the highest value during silking, and LP was in the V14 leaf stage. The correlation and principal component analysis showed that silking and the V14 leaf stage are the most important stages for yield, thus, higher attention must be paid to these stages in the LP and the activation of APX to achieve maximum yield.

Temperature and size-dependency of lumpfish (Cyclopterus lumpus) oxygen requirement and tolerance

Lumpfish are currently produced and utilized as cleaner fish, to control sea lice infestation rates in salmon net pens, but information on environmental requirements is still limited. This study aimed to determine the zone of environmental hypoxia for two relevant fish sizes (15 and 60 g) and temperatures (5 and 12 °C), using intermittent flow respirometry (referred to as 15:5, 15:12, 60:5, 60:12), and to investigate parameters of stress in response to acute changes in dissolved oxygen (DO, % air saturation) from normoxia to 47, 63, 98 (control), 148 and 194% O2 at 10 °C. The standard and maximal metabolic rates (SMR and MMR) were measured in normoxia (n = 8), and MMR was measured at 5–6 DO levels ranging from 20−160% O2 (n = 8 per DO) to define the upper and lower boundaries of the hypoxic zone (DOlim and DOcrit). SMR, MMR and the aerobic metabolic scope (AS) increased with temperature and decreased with fish size. Similar effects of temperature and size were found on DOcrit – DOlim ranges: 20–55 (15:5), 35–147 (15:12), 21–53 (60:5) and 22–89 (60:12) % O2 air saturation. Results from acute exposure tests resulted in elevated cortisol levels at 63 and 47% O2, although not statistically significant at 47% O2. Other parameters of hypoxic or hyperoxic stress (lactate, pH, osmolality, lipid peroxidation rates, catalase activity) were not affected. Results from the present study suggest that lumpfish may experience oxygen levels in sea cages that restricts metabolism, performance and induce hypoxic stress.

Effects of gamma radiation on the quality of Hass avocado fruits (Persea americana Mill.)

The Hass avocado is a crop with significant growth potential for Colombia as an export product; however, the country has reported quarantine-type pest insect species that limit its export. The objective of this research was to evaluate gamma radiation treatments of fruit in order to study its use as a post-harvest treatment. Gamma radiation levels were evaluated at 100, 200, 400 and 800 Gy and compared against a control without irradiation. The harvest maturity status was established as a blocking factor. The evaluation was carried out for 20 days after the application of the treatments, emulating conditions of refrigerated transport at 7 °C. The variables firmness, exocarp and mesocarp color, soluble solids, dry mass, respiration, and weight loss were analyzed longitudinally at five evaluation points, where time acted as an intrasubject factor in the analysis of longitudinal multivariate variance. The variables lipid peroxidation and ethylene production rate were evaluated only once at the end of the test. We found that the highest radiation treatment affected the color of the exocarp and the content of total soluble solids, while all treatments affected the respiratory intensity, increased lipid peroxidation, altering the color of the mesocarp, which is the tissue of greater commercial interest for fresh fruits. All the treatments affected the visual quality of the fruits independent of the point of harvest maturity.

THE EFFECT OF EGGPLANT JUICE TO THE THICKNESS OF AORTIC WALL IN WHITE RAT

Atherosclerosis is a complex chronic disease characterized by the accumulation of lipids within arterial walls. Delphinidin-3-(p-coumaroylrutinoside)-5-glucoside (nasunin), an anthocyanin, was isolated as purple-colored crystals from eggplant peels. Nasunin protection against induced lipid peroxidation in rat. The aim of study was to determine the effect of eggplant juice to the thickness of aortic wall of white rat with atherogenic diet. This true experimental laboratoric study using control group post test design performed in white rat that placed in pharmacologic laboratory of Brawijaya University. Sampling was carried out by completely random sampling with 25 rats for the total sample. Data were processed and analyzed using SPSS 16. Statistical test using one-way ANOVA and continuing with post hoc Tukey. The result showed there was a significant effect between positive control of diet atherogenic with the dosage I (1,3 gr/3 ml), dosage II (2,6 gr/3 ml), and dosage III (5,2 gr/3 ml) of eggplant juice to aorta wall thickness (p=0,000;p&lt;0,05) . We can conclude that the effective dosage of eggplant juice for reducing the progression of aortic wall thickening is dosage III (5,2 gr/3 ml).

Effect of Camellia sinensis on Fat Peroxidation and Ox-LDL in Rats.

Green tea (GT) is believed to have antioxidant properties and beneficial effects on the treatment of some diseases. However, few findings were found concerning the impact of GT on oxidative stress. In the present study, the protective influence of GT against the oxidative stress caused by hydrogen peroxide (H2O2) in rats was evaluated. The research groups included a control (Con) group and five groups supplemented with 10g GT(G1), 20g GT(G2), 1% H2O2(P), 1% H2O2and10g GT (GP1), as well as 1% H2O2 and 20g GT(GP2). The effects of GT and H2O2 administration on serum biochemical parameters, such as lipid profile, malondialdehyde (MDA), and oxidized low-density lipoprotein (Ox-LDL) were assessed. The findings of this research revealed that the usage of GT lowered the level of cholesterol, triglyceride, LDL, MDA, Ox-LDL and coronary risk index. Moreover, an increase in high-density lipoprotein and very-low-density lipoprotein (VLDL) was observed in subjects who received GT, compared to the rats of the P group. The baseline lipid profile and GT consumption with or without H2O2 were the same between the Con and GT-treated groups. Therefore, GT usage was found to be advantageous in reducing Ox-LDL and lipid peroxidation in rats. These results confirm the traditionally claimed benefits of GT for protection against lipid peroxidation and atherosclerosis.

Physiological and biochemical responses of Lawsonia inermis L. to heavy metal pollution in arid environments

Trace metal pollution is increasing worldwide due to human activities. In this study, we investigated the physiological and biochemical effects of heavy metals on henna plants (Lawsonia inermis L.) grown in oases situated at various distances from an industrial complex in arid southern Tunisia. Our results showed that leaves had higher mean Zn and Cu concentrations than roots, while the reverse was true for Pb and Cd. Such metal immobilization in root cells is related to an exclusion strategy. The biological concentration factor (BCF) of Cu was > 1 at all studied sites, indicating the potential of henna plants to accumulate Cu from the soil. Heavy metals affected various physiological parameters of henna, including water status, macronutrient content, and photosynthetic activity, and increased the rate of hydrogen peroxide (H2O2) production and lipid peroxidation in leaves and roots, indicating oxidative stress. Deleterious heavy metal effects induced biochemical mechanisms in henna plants by accumulating proline and soluble sugars and activated the antioxidant system by enhancing the activities of superoxide dismutase, catalase, and glutathione peroxidase. The magnitude of accumulation and activation was proportional to the concentration of heavy metals in the plant tissues. The stimulation of enzymatic and non-enzymatic antioxidant systems reflects the ability of henna plants to survive in highly polluted environments.

Response of Two Crop Plants, Zea mays L. and Solanum lycopersicum L., to Diclofenac and Naproxen

Among numerous contaminants, the ubiquitous occurrence of nonsteroidal anti-inflammatory drugs (NSAIDs) in the environment and their plausible harmful impact on nontarget organisms have made them one of the most important areas of concern in recent years. Crop plants can also potentially be exposed to NSAIDs, since the concentration of these pharmaceuticals is constantly rising in the surface water and soil. Our goal was to evaluate the stress response of two crop plants, maize and tomato, to treatment with selected NSAIDs, naproxen and diclofenac. The focus of the research was on the growth response, photosynthetic efficiency, selected oxidative stress factors (such as the H2O2 level and the rate of lipid peroxidation) as well as the total phenolic content, which represents the non-enzymatic protectants against oxidative stress. The results indicate that susceptibility to the NSAIDs that were tested is dependent on the plant species. A higher sensitivity of tomato manifested in growth inhibition, a decrease in the content of the photosynthetic pigments and a reduction in the maximum quantum efficiency of PSII and the activity of PSII, which was estimated using the Fv/Fm and Fv/F0 ratios. Based on the growth results, it was also possible to reveal that diclofenac had a more toxic effect on tomato. In contrast to tomato, in maize, neither the content of the photosynthetic pigments nor growth appeared to be affected by DFC and NPX. However, both drugs significantly decreased in maize Fv and Fm, which are particularly sensitive to stress. A higher H2O2 concentration accompanied, in most cases, increasing lipid peroxidation, indicating that oxidative stress occurred in response to the selected NSAIDs in the plant species that were studied. The higher phenolic content of the plants after NSAIDs treatment may, in turn, indicate the activation of defense mechanisms in response to the oxidative stress that is triggered by these drugs.

Brain region specificity in reactive oxygen species production and maintenance of redox balance

The brain produces various reactive oxygen species in enzymatic and non-enzymatic reactions as a by-product of metabolism and/or for redox signaling. Effective antioxidant system in the brain cells maintains redox balance. However, neurons and glia from some brain regions are more vulnerable to oxidative stress in ischemia/reperfusion, epilepsy, and neurodegenerative disorders than the rest of the brain. Using fluorescent indicators in live cell imaging and confocal microscopy, we have measured the rate of cytosolic and mitochondrial reactive oxygen species production, lipid peroxidation, and glutathione levels in cortex, hippocampus, midbrain, brain stem and cerebellum in acute slices of rat brain. We have found that the basal rate of ROS production is at its highest in brain stem and cerebellum, and that it is mainly generated by glial cells. Activation of neurons and glia by glutamate and ATP led to maximal rates of ROS production in the midbrain compared to the rest of the brain. Mitochondrial ROS had only minor implication to the total ROS production with maximal values in the cortex and minimal in the midbrain. The basal rate of lipid peroxidation was higher in the midbrain and hippocampus, while the GSH level was similar in most brain regions with the lowest level in the midbrain. Thus, the rate of ROS production, lipid peroxidation and the level of GSH vary across brain regions.

The effect of cisplatin-low-level laser therapy on cell viability and death of LNCaP prostate cancer cell line.

Prostate cancer, as a common male cancer, is a serious threat to men's health. In spite of extreme developments for increasing survival rate, there are still limitations about common treatment options such as surgical procedures, radiotherapy, and chemotherapy. We hypothesized that combination of two treatments would bring better clinical outcomes. Therefore, the aim of this study was to determine the effect of conjugated cisplatin and low-level laser treatment (LLLT) on the viability of LNCaP prostate cancer cell line. LNCaP cells were harvested in DMEM containing 10% FBS and 1% antibiotic. Confluent cells were treated with different concentrations of cisplatin and different wavelengths of low-level laser (LLL) alone and in combination. The relative IC50 and cell viability was evaluated using MTT assay. Analysis of lipid peroxidation rate was performed using lipid peroxidation assay kit. LDH activity was also carried out on the treated and control cells using LDH cytotoxicity assay kit. Our results showed that combination of cisplatin and LLLT could effectively decrease cisplatin-induced cytotoxicity as well as LNCaP cell viability. Cisplatin-LLLT combination led to a significant increase in the MDA content as the product of membrane lipid peroxidation. Analyzing the LDH activity under the effect of cisplatin-LLL combined treatment showed a remarkable increase in the enzyme activity. We conclude that applying the cisplatin-LLL combination therapy is promising as an effective anti-cancer treatment. This novel combination has a potential to attenuate adverse side effects of earlier monotherapy strategies.

Towards Aldehydomics: Untargeted Trapping and Analysis of Reactive Diet-Related Carbonyl Compounds Formed in the Intestinal Lumen.

Lipid peroxidation and subsequent formation of toxic aldehydes, such as 4-hydroxynonenal, is known to be involved in numerous pathophysiological processes, possibly including the development of colorectal cancer. This work aimed at the development of an untargeted approach using high-performance liquid chromatography coupled with high-resolution mass spectrometry (HPLC–HRMS) for tracking aldehydes in both suspect screening and untargeted methods in fecal water, representing the aqueous environment of colon epithelial cells. This original approach is based on the introduction of a characteristic isotopic labeling by selective derivatization of the carbonyl function using a brominated reagent. Following a metabolomics workflow, the developed methodology was applied to the characterization of aldehyde compounds formed by lipid peroxidation in rats fed two different diets differentially prone to lipoperoxidation. Derivatized aldehydes were first selectively detected on the basis of their isotopic pattern, then annotated and finally identified by tandem mass spectrometry. This original approach allowed us to evidence the occurrence of expected aldehydes according to their fatty acid precursors in the diet, and to characterize other aldehydes differentiating the different diets.

Oxidative Stress Does Not Influence Subjective Pain Sensation in Inflammatory Bowel Disease Patients.

Oxidative stress (OS) has been proposed as a significant causative and propagating factor in inflammatory bowel diseases (IBDs). Modulation of OS is possible through antioxidants and inhibition of oxidizing enzymes. Thirty-one IBD patients and thirty-two controls were included in the study. The aim was to examine the levels of OS in colonic tissue of IBD requiring surgical intervention and control group, and their association with pain intensity. Total antioxidant capacity (TAC), superoxide dismutase (SOD) and catalase (CAT) activity, glutathione (GSH) and oxidized glutathione (GSSG) levels, and glutathione peroxidase (GPX) activity as markers of antioxidant defense were determined. Cyclooxygenases activities (Total COX, COX-1 and COX-2) were measured as prooxidant enzymes. Thiobarbituric acid reactive substances (TBARS) concentrations were measured to evaluate lipid peroxidation. Disease activity was assessed, and each subject filled out VAS and Laitinen’s pain assessment scales. Correlation between the OS, pain intensity, disease activity parameters, C-reactive protein (CRP), number of stools passed daily, disease duration, and dietary habits was investigated. No TAC differences were found between the groups. A significant decrease of SOD activity and GSH and GSSG levels was seen in IBD patients vs. controls, while GPX activity was diminished significantly only in CD patients. CAT and COX-1 activity was increased, and COX-2 significantly decreased in IBD. TBARS were significantly higher in CD patients compared to control group. No correlation was found between pain scores, inflammatory status, disease activity, disease duration, or dietary habits and OS markers. In our study, OS did not influence pain sensation reported by IBD patients.

Light Quality-Dependent Regulation of Non-Photochemical Quenching in Tomato Plants.

Simple SummaryPhotosynthetic organisms, such as land plants, evolved to utilize available light and to use its energy to assimilate carbon dioxide and produce carbohydrates. However, the light intensity often exceeds the ability of plants to successfully utilize absorbed energy, thus inducing stress, manifested by an increased radical concentration inside plant cells and disruption of the inner structures, and consequently decreased plant yield. Plants solve this problem by using a mechanism termed non-photochemical quenching, by which they can dissipate the energy not used in photosynthesis. Modern agriculture, however, also involves indoor plant farming. For indoor plant farming LED-based lighting systems, with non-saturating light intensities, are suitable based on their restricted energy consumption. However, the composition of applied light should first be optimized to maximize its utilization. Our study examined the influence of monochromatic LEDs (red, green, and blue) on the photoprotective and photosynthetic properties of tomato plants. We indicate that monochromatic green light could be considered an important component of lighting systems to alleviate energy dissipation, while blue light enhances photosynthetic efficiency. Our study not only proves the crucial importance of spectrum optimization but also provides evidence that different light wavelengths modify photosynthetic and photoprotective properties.Photosynthetic pigments of plants capture light as a source of energy for photosynthesis. However, the amount of energy absorbed often exceeds its utilization, thus causing damage to the photosynthetic apparatus. Plants possess several mechanisms to minimize such risks, including non-photochemical quenching (NPQ), which allows them to dissipate excess excitation energy in the form of harmless heat. However, under non-stressful conditions in indoor farming, it would be favorable to restrict the NPQ activity and increase plant photosynthetic performance by optimizing the light spectrum. Towards this goal, we investigated the dynamics of NPQ, photosynthetic properties, and antioxidant activity in the leaves of tomato plants grown under different light qualities: monochromatic red (R), green (G), or blue (B) light (L) at 80 µmol m−2 s−1 and R:G:B = 1:1:1 (referred to as the white light (WL)) at 120 µmol m−2 s−1. The results confirm that monochromatic BL increased the quantum efficiency of PSII and photosynthetic pigments accumulation. The RL and BL treatments enhanced the NPQ amplitude and showed negative effects on antioxidant enzyme activity. In contrast, plants grown solely under GL or WL presented a lower amplitude of NPQ due to the reduced accumulation of NPQ-related proteins, photosystem II (PSII) subunit S (PsbS), PROTON GRADIENT REGULATION-LIKE1 (PGRL1), cytochrome b6f subunit f (cytf) and violaxanthin de-epoxidase (VDE). Additionally, we noticed that plants grown under GL or RL presented an increased rate of lipid peroxidation. Overall, our results indicate the potential role of GL in lowering the NPQ amplitude, while the role of BL in the RGB spectrum is to ensure photosynthetic performance and photoprotective properties.

Combination of cisplatin treatment and photodynamic therapy attenuates cisplatin-induced cell toxicity in A2780 and A2780-CP cervical cancer cell lines.

Cervical cancer is recognized as a serious worldwide health problem. Despite various achievements for cervical cancer treatment, there are still shortcomings that lead to severe side effects. Combination therapy is fast becoming a key and promising treatment strategy, diminishing chemotherapy-mediated side effects. The objective of this study was to determine the effect of combined cisplatin treatment and photodynamic therapy (PDT) on the cervical cancer recovery. In this study, A2780 and A2780-CP cell lines were cultured in the Dulbecco's modified eagle medium (DMEM) enriched with 10% FBS and 1% antibiotic. Both cell lines were treated with cisplatin, photodynamic light (laser with methylene blue as a photosensitizer agent), and the combination of cisplatin treatment and PDT. Half maximum inhibitory concentration (IC50) was calculated for each treatment by the use of tetrazolium salt assay. Both cell lines were examined for cell membrane lipid peroxidation rate. Our findings showed that combination of cisplatin treatment and photodynamic therapy leads to two-fold decreased cisplatin IC50. Results showed that cisplatin and photodynamic light combination could effectively reduce A2780 and A2780-CP cell viability (p-value < 0.0001). Moreover, combined cisplatin and photodynamic therapy results revealed significantly increased cancer cell membrane destruction through increased lipid peroxidation, resulting in surged MDA content. Our conclusion is that combination of cisplatin and photodynamic therapy can be used as an effective and convenient treatment strategy without considerable side effects.