Infestation Rates Research Articles

Recombinant Breeding in Barnyard Millet (Echinochloa frumentacea. L): Pioneering High-yield Varieties for Sustainable Agriculture

The development of new crop varieties, including barnyard millet, typically spans 6 to 9 years, a process that began with breeding efforts initiated in 2008. The breeding work involved initial crossings, followed by selection starting from the F2 generation, which achieved stabilization in the F5 generation with the identification of the best plants. The resulting high-yielding, medium-maturing barnyard millet variety, DHBM-93-3, was developed at the Agricultural Research Station in Hanumanamatti, under the University of Agricultural Sciences, Dharwad. This variety matures in 85 to 90 days, features an erect growth habit with a height ranging from 132 to 147.5 cm, and produces bold, oval-shaped grains of gray color. DHBM-93-3 has demonstrated an impressive grain yield of 23.20 q/ha and a fodder yield of 6.33 t/ha, along with remarkable tolerance to shoot fly infestation (0.0%). Notably, this variety outperformed national checks VL-172 and VL-207, with yield increases of 27.17% and 12.59%, respectively. In preliminary and station trials conducted between 2010 and 2013, DHBM-93-3 achieved an average seed yield of 39.24 q/ha, significantly exceeding RAU-11 and VL-207 by 42.17% and 16.82%. Over a three-year evaluation period, it consistently yielded 23.20 q/ha, surpassing VL-172 and VL-207 by 27.15% and 12.59%, respectively. The varieties exceptional adaptability was highlighted by significant yield increases across various states, particularly in Andhra Pradesh, where it exceeded VL-172 by 108.5%. DHBM-93-3 also exhibited superior disease resistance, with a mean grain smut incidence of 2.46%, compared to 10.06% for VL-172 and 15.93% for VL-207, as well as complete resistance to head smut. Furthermore, it showed outstanding resilience against shoot fly infestation, maintaining a consistent infestation rate of 0.0%. Nutritionally, DHBM-93-3 excelled in zinc (36.8 mg/kg), iron (12.5 mg/kg), and calcium (14 mg/kg) content, although its protein content was slightly lower than that of VL-172. DHBM-93-3 represents a highly productive and resilient variety, capable of enhancing food security and addressing micronutrient deficiencies, thus positioning it as an excellent choice for cultivation across diverse agro-climatic regions of India.

Effect of Two Biofertilisers Based on Endophytic Fungi on Fall Armyworm Infestations in Maize Production in the Kara Region of Togo, West Africa

Aims: The aim of the study was to assess the efficacy of two biofertilisers based on endophytic fungi on infestation rates, foliar damage and maize yield losses caused by the armyworm, Spodoptera frugiperda, a worst maize pest. Study Design: Completely Randomized Design (CRD) with three types of treatments. Place and Duration of Study: Agronomic Experimentation Station of Tchitchao, Kara (Togo), between October 2019 and March 2020. Methodology: A complete randomized block design was used with three types of treatments: (i) maize seeds inoculated with Glomus sp. strain 142, (ii) maize seeds inoculated with Mycotri, (iii) maize seedlings fertilized with NPK and urea and finally (iv) a control consisting of seeds and/or seedlings of maize neither fertilized nor inoculated. Results: The results show that the plots treated with endophytic fungi recorded significantly lower infestation rates compared with the fertiliser treatments and the control. The highest infestation rates were 54% at 38 days after sowing (DAS) and 45% at 31 DAS for treatments with 5.35 x 105 spores/ha Glomus sp. and 0.38 kg/ha Mycotri, respectively, compared with 78% at 31 DAS and 62% at 38 DAS for fertiliser treatments and the control. The highest level of leaf damage, 2.92, was observed at 33 DAS on the plots fertilised with fertiliser, compared with scores of 2.53 and 2.38 for the plots mycorrhised with Glomus sp. and Mycotri, respectively. Yields were significantly higher in the fertilised plots (3.87 t/ha), followed by the mycorrhised plots (2.62-3.01 t/ha) and the control (1.73 t/ha). Conclusion: The endophytic fungi enabled the plants to tolerate fall armyworms attacks better than the controls, offering an alternative to chemical fertilisers and an effective option for managing soil fertility and controlling Spodoptera frugiperda.

Breeding for Pest and Disease Tolerance High Yielding Variety (DHLM-36-3-1) in Little Millet (Panicum sumetrense. L)

Developed from 2008 to 2013 and evaluated between 2010 and 2014, the high-yielding medium-maturing little millet variety DHLM-36-3-1 was officially released and notified in 2018, showing its potential as an excellent variety. This cultivar, developed at the Agricultural Research Station in Hanumanamatti, University of Agricultural Sciences, Dharwad, is well-suited to the state of Karnataka. It matures in 85 to 90 days and features an erect growth habit, reaching heights of 112 to 130 cm, with bold, oval-shaped grains of light black color. DHLM-36-3-1 demonstrated a grain yield of 14.29 q/ha and a fodder yield of 5.6 t/ha, showing good tolerance to shoot fly infestation (15.50%) compared with the national check JK-8, which recorded a 20.34% infestation rate. Furthermore, DHLM-36-3-1 outperformed both national checks OLM-203 and JK-8 by 9.59% and 11.21%, respectively, in terms of grain yield. Similarly, the newly developed variety DHLM-14-1 has yielded promising results across various trials conducted throughout India, demonstrating its potential as a high-yielding and nutritionally rich option for farmers. Over three growing seasons, DHLM-14-1 achieved an impressive average seed yield of 35.12 q/ha, exceeding the local check TNAU-63 by 18.56% and the national check OLM-203 by 12.06%. It has consistently performed well in All India Coordinated Trials, showing adaptability across diverse agro-climatic conditions, with notable yield improvements in states such as Andhra Pradesh, Jharkhand, and Tamil Nadu. Nutritional assessments highlight DHLM-14-1's superior mineral profile, boasting higher levels of zinc (4.06 mg/100 g) and calcium (3.45 mg/100 g) compared to national checks, while its iron content (16.2 mg/100 g) significantly surpasses that of OLM-203. Additionally, disease resistance evaluations revealed that DHLM-14-1 maintained lower incidence rates for grain smut and brown spots, with competitive resistance to shoot fly. Collectively, these findings emphasize DHLM-14-1's vital role in promoting sustainable millet production, enhancing food security and farmer livelihoods, supporting integrated pest management strategies, and improving the nutritional quality of staple diets in areas with micronutrient deficiencies.

Entomological surveillance of container-breeding mosquitoes focusing on Aedes (Stegomyia) (Diptera: Culicidae)vectors along altitudinal range in Nepal.

Mosquitoes are a significant public health concern due to their role in transmitting various diseases. This study aimed to investigate mosquitoes' diversity, abundance, and ecological aspects, mainly focusing on Aedes (Diptera: Culicidae)mosquitoes, in central Nepal. The research explored variations across regions, seasons, altitudes, and years. Fieldwork for mosquito collection was conducted between May 2022 and October 2023. Dipping and pipetting methods were employed to collect larvae and pupae, whereas Biogents-Mosquitaire trap captured adult mosquitoes. A total of 7,223 (3,640 larvae and 3,583 adults) mosquitoes, belonging to 8 genera and 18 species, were collected and analyzed. Additionally, a survey examined 5,941 wet containers of 20 different types to assess potential breeding sites. The study revealed Culex pipiens (Linnaeus, 1758)(34.13%) and Aedes albopictus (Skuse, 1895)(27.36%) as the most abundant species. Interestingly, larvae were predominantly Aedes spp. (66.13%), whereas only 13.76% of adults belonged to this genus. Mosquito abundance varied across locations and altitudes, with Siwalik region (331-700 m asl) exhibiting the highest numbers. The monsoon season showed the highest overall abundance (1,492). Used tires were identified as significant breeding sites for Aedes mosquitoes, and infestation rates were higher in shaded containers. Seasonal analysis showed the House Index (HI) reaching its peak (10.92%) and the Breteau Index (BI) reaching 23.08% during the monsoon. Conversely, the Container Index (CI) reached its highest point (37.67%) in the post-monsoon season. The results emphasize the need for comprehensive disease prevention strategies at local and national levels, including public awareness campaigns, to address mosquito-borne illnesses in this famous tourist region.

Population fluctuation of the whitefly Bemisia tabaci Gennadius, 1889 (Homoptera - Aleyrodidae) on vegetable crops and effect of two entomopathogenic fungi Verticillium sp. and Paecilomyces sp.

The cotton or sweet potato whitefly, Bemisia tabaci, is a major pest in Algeria, affecting various greenhouse-grown crops. This study aimed to monitor B. tabaci population dynamics on tomatoes, peppers, zucchinis, and strawberries to understand its infestation patterns. The methodology involved regular monitoring of pest activity on these crops and a laboratory bioassay to assess the efficacy of two entomopathogenic fungi, Verticillium sp. and Paecilomyces sp., in controlling the whitefly. Results revealed that B. tabaci was most prevalent on tomatoes, with a 43.73% infestation rate, followed by peppers at 24%, zucchinis at 19.14%, and strawberries at 13.11%. In addition, laboratory trials were conducted to evaluate the efficacy of two entomopathogenic fungi, Verticillium sp. and Paecilomyces sp., in controlling the whitefly. Verticillium sp. showed higher efficacy, achieving a 73% mortality rate, while Paecilomyces sp. resulted in a significantly lower mortality of 23%. These findings suggest that Verticillium sp. holds promise as a biological control agent for managing B. tabaci in Algeria, particularly on tomato crops.

Ectoparasite infection levels differ between fish from upwelling-exposed and sheltered rocky reefs areas in Brazil

Ubiquitous and abundant within marine ecosystems, parasites play essential ecological roles such as shaping host population dynamics, altering competition between species, and influencing energy flows through communities. Their diversity and population dynamics are demonstrably shaped by both seasonal and geographic variations. These variations have been often explored at broad spatial scale. However, parasite communities can exhibit significant disparities even at small spatial scales, driven by factors such as wave exposure, temperature fluctuations, and benthic habitat composition. We investigated how crustacean parasites of fish – caligids and gnathiids - differed between two distinct habitats, which are separated solely by a few kilometres, at Arraial do Cabo, Brazil. These two habitats were characterised by a sheltered embayment (hereinafter referred to as “inside”) or an exposed upwelling habitat (hereinafter referred to as “outside”). Individual fish from four species were examined in both habitats. We found that the infestation rate of caligids varied among fish species and, gnathiids varied between the two sampling sites. Gnathiids were absent from fish outside, while they were present on fish inside the embayment. This disparity suggests a critical role of local environmental factors in shaping gnathiid distribution. Potential drivers include temperature fluctuations, substrate composition, and wave exposure, which differed markedly between the two sites. Conversely, caligid parasites infected fish in both locations. While environmental factors may also influence caligid abundance, they appear to exhibit greater tolerance compared to gnathiids. These findings emphasize the importance of incorporating fine-scale environmental heterogeneity when investigating parasite distribution patterns.

Assessing Cashew Orchards Management Practices and Infestation rates of Apate terebrans in Orchards in Burkina Faso, West Africa

Assessing Cashew Orchards Management Practices and Infestation rates of Apate terebrans in Orchards in Burkina Faso, West Africa

Agroecological importance of smooth brome in managing wheat stem sawfly (Hymenoptera: Cephidae) via associated braconid parasitoids.

Wheat stem sawfly (WSS), Cephus cinctus Norton (Hymenoptera: Cephidae), is a major pest of cereal crops throughout the Northern Great Plains of North America. Native parasitoids, Bracon cephi (Gahan) and B. lissogaster Muesebeck (Hymenoptera: Braconidae), play a key role in suppressing WSS populations and limiting associated damage. Smooth brome grass (Bromus inermis Leyss.) serves as a potential trap reservoir for WSS when grown in areas surrounding wheat (Triticum aestivum L.) fields in Montana. Its unique biology allows it to support high WSS infestation while promoting significant larval mortality throughout the growing season. Late-season WSS survivors can then serve as hosts for WSS parasitoids. Our study investigated smooth brome as a host refuge for WSS parasitoids. We measured WSS larval infestation and survival rate inside smooth brome grown within WSS-inclusion cages, finding a maximum infestation of 66.5% and an end-of-year WSS survival of 5.7%. In addition, we collected stems from sites in central and north-central Montana to measure the WSS infestation and parasitoid prevalence in wheat and adjacent smooth brome. WSS infestation was high in both Big Sandy (64.5% smooth brome, 65.7% adjacent wheat) and Moccasin, MT (50.6%, 38.6%). Year-end WSS larval mortality was 43.6% greater in smooth brome compared to adjacent wheat at both field sites, but both hosted similar numbers of WSS parasitoids. This research underscores the importance of smooth brome in providing a sustainable host refuge for WSS parasitoids and highlights its significant role in supporting the economics of wheat cultivation.

Evidence of circulating recombinants between deformed wing virus and Varroa destructor virus-1 in honey bee colonies in Türkiye.

Deformed wing virus (DWV), which is an important honey bee virus transmitted by Varroa destructor (V. destructor), causes colony losses in honey bee colonies. This study aimed to investigate the prevalence and genetic diversity of DWV in honey bees in Türkiye and to determine the role of V. destructor in the transmission of the genetic variants of DWV. Honey bee samples were collected from 62 apiaries, by simple random sampling, during March 2022 and April 2023. The presence of V. destructor in collected bee samples was examined using a stereo microscope. Real-time RT-PCR was used for the detection of DWV-A and DWV-B (Varroa destructor virus-1 (VDV-1)) viruses. Genetic characterisation of the positive samples was conducted by sequencing polyprotein genomic region. Considering the V. destructor infestation rate of 3% as relevant, out of the 62 apiaries examined, 17 (27.4%) were positive. However, DWV-A and VDV-1 specific RNA was not detected in V. destructor samples. VDV-1 specific RNA was detected in 6.5% (4/62) of the apiaries, whereas DWV-A was not detected in the sampled apiaries. Phylogenetic analysis showed that isolates detected in this study were located in a separate cluster from previously characterised DWV-A and VDV-1 isolates. According to RDP4 and GARD analyses, DWV-VDV-1 recombination breakpoints were detected in field isolates. To the best our knowledge, this is the first report of the presence of VDV-1-DWV recombinants in Türkiye. Further studies are needed to determine the impact of VDV-1-DWV recombinants and their virological and antigenic properties.

Managing the parasitic honey bee mite Tropilaelaps mercedesae through combined cultural and chemical control methods

The western honey bee (Apis mellifera) is severely impacted by the parasitic Tropilaelaps mercedesae mite, which has the capacity to outcompete Varroa destructor mites (the current leading cause of colony losses) and more rapidly overwhelm colonies. While T. mercedesae is native to Asia, it has recently expanded its geographic range and has the potential to devastate beekeeping worldwide if introduced to new regions. Our research exploited the dependence of T. mercedesae on developing honey bees (brood) by combining a cultural technique (brood break) with U.S. registered chemical products (oxalic acid or formic acid) to manage T. mercedesae infestation. To evaluate this approach, we compared four treatment groups: (1) Brood Break; (2) Brood Break + Formic Acid (FormicPro®); (3) Brood Break + Oxalic Acid dribble (Api-Bioxal®); and (4) untreated Control. We found that the mite infestation rate of worker brood in Control colonies rose from 0.4 to 15.25% over 60 days, whereas all other treatment groups had infestation rates under 0.11% on Day 60. Mite fall assessments showed similar results, whereby Control colonies had 15.48 mites fall per 24 h on day 60 compared to less than 0.2 mites for any other treatment group. Evaluation of colony strength revealed that Brood Break + Formic Acid colonies had slightly reduced adult honey bee populations. No treatment eliminated all mites, so additional measures may be needed to eradicate T. mercedesae if detected in countries that do not currently have T. mercedesae.

The Susceptibility of Malaysian Rice Brands To Sitophilus oryzae and The Potential Application of Zinc Oxide Nanoparticles For Control Purposes

The rice weevil, Sitophilus oryzae, is an insect pest that can pose severe harm to stored grains. Rice weevils, both adults and larvae, cause significant economic losses because they can infest a wide range of plants and stored goods. Nanoparticle insecticides are created and studied to replace the overly-used synthetic chemical insecticides. The purpose of this study is to investigate the susceptibility of several commercialized rice brands towards S. oryzae infestation and to assess the toxicity of different types of zinc oxide (ZnO) against S. oryzae. The study involved twelve rice brands, and S. oryzae infestation rates were observed in the laboratory for 17 weeks. The infestation rates were obtained at the end of the observation period. To obtain the required dosages of 0.1, 0.2, 0.4, and 0.8% (w/w), four different concentrations of each type of ZnO nanoparticle that had undergone various reactivity modifications were mixed with rice for bioassays test. S. oryzae has been proven to be susceptible to almost all commercialized rice brands studied in this research. Based on the data, it can be concluded that the ZnO nanoparticle modified with gamma-ray is the most effective at controlling S. oryzae, as 100% mortality was seen after three days of treatment at concentrations between 0.4 to 0.08% (w/w). The slow emergence of pests in monitored rice brands is attributed to the protectants and their residue, which work by eradicating eggs and adults that have consumed the grain. Zinc oxide type 7A is the most toxic towards S. oryzae because of its exposure to gamma-ray which has the highest energy compared to neutron and beta rays. This results in the release of more active molecules to act on the pest.

Susceptibility of apple varieties to infestation by aphids (Hemiptera: Aphididae) in Southern Bulgaria

Aphids regularly infest apple orchards and are considered as one of the most damaging species, especially in humid conditions. In 2021-2023, the collection of different species and the rate of infestation by aphids on different apple varieties were studied in the region of Plovdiv, Bulgaria. Among the four aphid species identified in the apple orchard, Aphis spiraecola and Dysaphis plantaginea were predominant, followed by Aphis pomi and Dysaphis devecta. The rate of infestation varied significantly among the apple varieties. The Gala Resistant was the most infested by the aphids with a population coefficient (K) 302.5, followed by the Crimson Crisp – 124, the Pinova – 91, and the Red del Mestar – 52.5. The least infested variety was the Super Chief – 0.9, followed by the Modi – 2.2, the Fujion – 2.8, the Enterprise – 7.2 and the Rosella – 19. Keywords: apples, varietal susceptibility, aphids

Exploring the Genetic and Morphological Diversity of Argulus Ectoparasite Infecting the Aquaculture and Ornamental Fish in Tripura

Background: The present study screened for isolation of Argulus spp. infecting fishes based on the report of causing substantial economic losses both in aquaculture ponds and ornamental shops across several locations in Tripura, India. Methods: Identification of Argulus spp. was performed based on morphological, and molecular techniques. The molecular identification of Argulus spp. was performed using universal eukaryotic 18S rRNA genetic markers. Prevalence analysis was performed to determine the infestation rate of Argulus spp. in different hosts. Result: Seven A. japonicus, one A. coregoni and one A. foliaceus isolates were identified based on molecular and morphological features. In the present study it was found that A. japonicus infected a wide range of host species, including Goldfish, Milky Koi carp, Mrigal, Rohu, Catla and Subunkin. It was the first instance of A. japonicus infecting milky koi carp both in India and globally. On the other hand, A. foliaceus and A. coregoni infected only tiger oscar and goldfish respectively, indicating a rather narrow range of host diversity.

Susceptibility of Aromatic and Non- aromatic Rice to Sitophilus oryzae L. and their Influence on the Insect and Infestation Rate under Sub-Himalayan Plains of West Bengal

Relative susceptibility of local aromatic and non-aromatic rice grains to rice weevil, Sitophilus oryzae L. (Curculionidae, Coleoptera) was studied under laboratory conditions in the sub-Himalayan plains of West Bengal in two seasons (viz. winter and spring-summer) during 2020-2021. Results revealed that after 9 weeks of storage, the highest mean grain weight loss due to S. oryzae varied from 14.30% to 20.89% in the two types of rice grains in two seasons. The corresponding highest mean grain damage % ranged from 36.89 % to 41.56%. The grain weight loss and grain infestation % were always higher in the aromatic rice (Uttar Sugandhi) compared to non-aromatic rice (Jaldhepa) and also in the spring-summer season than the winter months. Carbohydrate and protein content of grains were reduced to the extent of 9.08% to 15.28% and 18.62% to 32.58% in aromatic and non-aromatic rice during winter and spring-summer seasons respectively as a result of infestation of S. oryzae. During winter and spring-summer seasons, from 5 pairs of adult weevils, a total of 188.60 and 142.20 and 264 and 237 number of adults were produced in aromatic and non-aromatic rice cultivars respectively at 9 weeks after their release. The adult population was always female biased.

Parasitic contamination of environmental objects with nematode eggs of Trichuris genus in the city of Poltava

The spreading of dog digestive tract helminthoses, including trichurosis, still remains a topical problem, where one of the factors of significant infestations’ spreading is the contamination of the environment with parasites’ eggs, which can be preserved in the environment for a long time and cause infestation of susceptible animals. Therefore, one of the important factors in maintaining veterinary well-being in relation to dog trichurosis is establishing the contamination level of environmental objects by propagative stages of nematode development. The purpose of the research was to determine contamination indicators of sand and soil in the city of Poltava with nematode eggs of Trichuris genus. The studies were conducted on the basis of the laboratory of parasitology of Poltava State Agrarian University. Sand samples from sandboxes located on the territory of the city of Poltava, as well as soil from the territories adjacent to them were studied. The main contamination indicators were the extensive contamination index and intensive contamination index. It was revealed by the conducted research that 61 out of 90 sandboxes in the city of Poltava were contaminated with nematode eggs of Trichuris genus, where the extensive contamination index made 67.78 %, and the intensive contamination index made 195.79±18.41 eggs/kg. The territory of Kyiv district was the most contaminated with nematode eggs, where 25 out of 30 sandboxes were contaminated with propagative stages of trichurises development, and the level of parasites’ contamination was 83.33 % and 218.91±17.27 eggs/kg. It was found that the most infected sand was taken from the surface at the edges of the sandbox, near its walls, where the extensive and intensive contamination indices made 41.11 % and 320.27±35.43 eggs/kg, respectively. Also, high rates of parasitic infestation were found during studying the soil, taken from the surface at a distance of 1 m from the sandbox, where, on the average, the extensive contamination index was 46.67 %, and the intensive contamination index was 263.90±28.87 eggs/kg. With an increase in the sampling depth, the indicators of contamination with trichurises’ eggs decreased and amounted to: on the surface – 36.67–46.67 % and 120.37–320.7 eggs/kg, at a depth of 5 cm – 14.44–30.00 % and 80.77–274.07 eggs/kg, and at a depth of 10 cm – 8.89–20.00 % and 62.50–125.00 eggs/kg. The obtained results of parasitological studies prove that the territory of sandboxes is a real factor in the transmission of invasive agents, which must be taken into account in assessing the risks of infecting dogs with trichurosis causative agent.

Epidemiological Study on Three Hundred Patients of Scabies in the Industrial Area of Dhaka, Bangladesh

Background: Scabies is a common and highly contagious skin disease caused by a mite called sarcoptes scabiei. Objective: The aim of this study was to analyze the epidemiological factors associated with scabies infection in an industrial area. Methodology: Write the study design, study place, study period and study population here. It was considering age, sex, occupation, economical condition, educational status, family history of scabies of the selected patient. Results: The age group of 1 to 10 years (28.66%) and low socioeconomic condition (58.66%), illiterate (Primary 44.00%), overcrowding living (Madrasah 37.33%) showed the highest infestation rates. Write more findings here Conclusion: In conclusion an improvement of socio-economic conditions, improve living facilities, education, social awareness may contribute to a reduction in the number of scabies infections. Journal of Monno Medical College June, 2024; 10 (1):13-16

Infestation Rates, Seasonal Abundance of Aphids and Relationships with Some Environmental Factors in Different Forest Habitats in Southwestern Türkiye

Aim of study: It was to determine the infestation rate and seasonal abundance of aphids and their relationships with some environmental factors in forests. Area of study: The study was conducted in Pinus nigra, P. brutia, Cedrus libani, Abies cilicica, Juniperus spp., Quercus spp. and Robinia pseudoacacia forests (34 sampling areas) in Isparta. Materials and methods: Systematic sampling was carried out monthly throughout April-October in 2019-2020. As aphid density and infestation rates were determined, 30 cm shoots were taken from 10 trees. The slope, aspect, elevation, forest characteristics, daily minimum, average, and maximum temperature and total precipitation were recorded. Main results: Abundance were highest in P. nigra, Quercus and Robinia pseudoacacia forests in both years. There was a significant correlation between abundance and development stages (p

New Methods and Challenges for IPM in Archives, Modern and Historic Libraries

ABSTRACT In this article the most important aspects of IPM, new challenges like climate change, new pests and updated methods of detection are presented. The grey silverfish has already resulted in many problems in archives and libraries across Europe. To set priorities and evaluate a large storage building with many rooms, we present a pest index for detecting high infestation rates and hotspots in the building. For the biscuit beetle we present two new detection methods: The use of a scent detecting (or sniffing) dog trained to detect the larvae of the beetle and the Insect Activity Detection System (IADS), a new technology that uses the acoustical emissions of the larvae feeding on the material. The last aspect of this paper is climate change: in the future higher temperatures, extreme weather events and introduced species will be new challenges for libraries and archives.

Enhancing yield and economic benefits through sustainable pest management in Okra cultivation.

Okra (Abelmoschus esculentus) is a prominent vegetable crop in Asia, confronting persistent threats from pests such as leafhoppers, whiteflies, and shoot and fruit borers. Conventional chemical control methods, despite their adverse ecological effects, remain the primary approach for pest management. Indiscriminate chemical use has led to reduced biodiversity among natural predators and the disruption of food webs in ecosystems. To address these challenges, this study assessed the efficacy of integrated (IM) and biointensive (BM) pest management modules in comparison to conventional chemical methods (CM) for mitigating insect damage to okra leaves and fruits, and subsequently, their impact on okra yield. Our result revealed that the BM exhibited the least effectiveness but outperformed untreated control plots significantly. In contrast, both IM and CM significantly reduced damage from sap-sucking insects and borer pests. Notably, plots treated with the chemical module found decreased populations of natural enemies. The IM demonstrated the lowest fruit infestation rate (5.06%), yielding the highest crop production (8.97 t ha-1), along with the maximum net return (Indian Rupees: 44,245) and incremental cost-benefit ratio (3.31). Thus, the study suggested that the implementation of integrated pest management practices can result in higher okra yields and greater economic benefits. These findings shed light on the potential of sustainable agricultural practices as a safer and more economically viable alternative to chemical-intensive pest control in okra cultivation.

Protozoan intestinal invasions in military personnel of the Northern region of Ukraine

Objective — to study the prevalence and spectrum of intestinal parasitic infestations (IPI) agents in groups of military personnel of the Northern region of Ukraine, which differed according to the criteria of having symptoms of gastrointestinal disorders and passing the serving in a combat zone. Materials and methods. From February to November 2023, the study covered 302 servicemen during inpatient medical care at the Military Medical Clinical Center of the Northern Region of Ukraine. Detection and identification of intestinal parasites in samples of feces (SF) was carried out by classical methods of microscopy using the enrichment procedure (formalin‑ethyl acetate sedimentation) and the preparation (from each enriched sediment) of four smears: two thick wet with 50% aqueous solution of glycerol and D’ Anthony iodine solution; two thin, fixed, permanently stained with Whitley’s modified trichrome (mWT) or Heidenhain’s iron hematoxylin (HIH) and the modified Ziehl‑Neelsen acid method (mZN). Smear microscopy results were evaluated according to the criteria of the US Clinical and Laboratory Standards Institute. Results. It was established that the level of prevalence of IPI among the examined servicemen reached 10.6% with variation in the value of this indicator in different groups of soldiers from 2.2% to 12.6%. A specific share of protozoan monoinvasions in soldiers reached 65.6%, and mixed invasions (with two parasites at the same time) — 34.4%. Soldiers with symptoms of gastrointestinal diseases had significantly higher rates of IPI than did asymptomatic. In addition, the infestation rates among soldiers were higher in the cold season (October‑February) with a seasonality index of 1.9. All cases of IPI in servicemen (n=32) were caused by protozoan parasites (n=39), in the etiological spectrum of which the vast majority (84.6%) were pathogenic species (Blastocystis sp., Dientamoeba fragilis, Giardia lamblia), and a smaller proportion (15.4%) were commensal species (Chilomastix mesnili, Iodamoeba butschliі). Among pathogenic protozoa (n=33), Blastocystis sp. (51.5%) and D. fragilis had the highest proportion (42.4%), G. lamblia (6.1%) had significantly less one. Conclusions. Optimizing the management tactics of military medicine to reduce the negative impact of IPI on the health and combat capacity of servicemen requires knowledge about the prevalence and spectrum of pathogens of invasions among specific groups of soldiers. A necessary condition for increasing the efficiency of microscopic detection/identification of protozoan parasites (primarily D. fragilis) in the SF is the production of permanently stained (mWT or HIH) smears. Microscopic examination of such smears at high resolution (100ґ objective, oil immersion) provides an increased incidence of detection/identification of Blastocystis sp. by 47.1% and D. fragilis by 100%.