Rates Of Imazethapyr Research Articles

Efficacy of glyphosate, glufosinate, and imazethapyr on selected weed species

Experiments were conducted to determine the efficacy, absorption, and translocation of glyphosate, glufosinate, and imazethapyr with selected weed species. In the greenhouse glyphosate, glufosinate, and imazethapyr were applied at 0.25, 0.5, and 1 times their label rates of 1,121, 410, and 70 g ha−1, respectively, on 10- to 15-cm black nightshade, common waterhemp, eastern black nightshade, field bindweed, giant ragweed, ivyleaf morningglory, prairie cupgrass, velvetleaf, and yellow nutsedge. Glyphosate applied at the 1-time rate caused injury greater than or similar to injury from the 1-time rate of glufosinate or imazethapyr on black nightshade, common waterhemp, eastern black nightshade, field bindweed, giant ragweed, prairie cupgrass, and velvetleaf. The 1-time rate of glufosinate injured ivyleaf morningglory and yellow nutsedge more than did the 1-time rate of glyphosate or imazethapyr. Under field conditions glyphosate caused the greatest injury to common waterhemp, prairie cupgrass, and velvetleaf across plant growth stages. Giant ragweed and ivyleaf morningglory injury was more dependent on growth stage, with the 15- and 30-cm growth stages more susceptible to glyphosate than to glufosinate or imazethapyr. Differential response of these weed species may be caused by differences in herbicide translocation. Glyphosate was translocated more in both giant ragweed and ivyleaf morningglory, and these species were injured more by glyphosate than by glufosinate or imazethapyr at the larger growth stages.

Ethylmethanesulfonate saturation mutagenesis in Arabidopsis to determine frequency of herbicide resistance.

Plant resistance to glyphosate has been reported far less frequently than resistance to sulfonylurea and imidazolinone herbicides. However, these studies tend to be anecdotal, without side by side comparisons for a single species or natural isolate. In this study, we tested the frequencies of resistance of three herbicides in a controlled ethylmethanesulfonate (EMS) saturation mutagenesis experiment, allowing a direct comparison of the frequencies at which resistant mutant plants arise. The 100% growth inhibition dose rates of glyphosate, chlorsulfuron (a sulfonylurea herbicide), and imazethapyr (an imidazolinone herbicide) were determined for Arabidopsis. Populations of EMS-mutagenized M(2) seedlings were sprayed with twice the 100% growth inhibition dose of glyphosate, chlorsulfuron, or imazethapyr, and herbicide-resistant mutants were identified. Although there were no glyphosate-resistant mutants among M(2) progeny of 125,000 Columbia and 125,000 Landsberg erecta M(1) lines, chlorsulfuron resistance and imazethapyr resistance each appeared at frequencies of 3.2 x 10(-5). Given the observed frequency of herbicide resistance mutations, we calculate that there are at least 700 mutations in each EMS-mutagenized Arabidopsis line and that fewer than 50,000 M(1) lines are needed to have a 95% chance of finding a mutation in any given G:C base pair in the genome. As part of this study, two previously unreported Arabidopsis mutations conferring resistance to imidazolinone herbicides, csr1-5 (Ala-122-Thr) and csr1-6 (Ala-205-Val), were discovered. Neither of these mutations caused enhanced resistance to chlorsulfuron in Arabidopsis.

Interactions between glyphosate and imazethapyr on four annual weeds

Greenhouse and field experiments were conducted to evaluate the efficacy of various combinations of imazethapyr (0, 23, 46, and 70 g ai/ha) with glyphosate (0, 210, 420, 630, and 840 g ae/ha) on Setaria faberi, Amaranthus rudis, Abutilon theophrasti, and Ipomoea hederacea control. Additivity was the most frequently observed interaction and no synergistic interaction occurred throughout this study. The combination of imazethapyr with glyphosate at 210 g/ha caused an antagonistic response on Setaria faberi. Glyphosate at 420 g/ha with or without imazethapyr provided at least 95% control of Setaria faberi. The interaction between glyphosate and imazethapyr was additive on Amaranthus rudis control. Eight of the twenty-one herbicide combinations were antagonistic on Abutilon theophrasti control. Antagonistic interactions occurred when 46 or 70 g/ha of imazethapyr was added to 420 or 630 g/ha of glyphosate; while no antagonistic interactions were noted when glyphosate rate was 840 g/ha. The interactions on Ipomoea hederacea control were additive when the glyphosate rate was at least 420 g/ha. Glyphosate at 210 g/ha plus imazethapyr at 46 or 70 g/ha caused antagonistic interactions on Ipomoea hederacea control. Weed control tended to be more variable when the glyphosate rate was 210 g/ha and the imazethapyr rate was 46 or 70 g/ha. In general, the addition of imazethapyr to low rates of glyphosate improved control of Amaranthus rudis and Ipomoea hederacea and did not improve control of Setaria faberi and Abutilon theophrasti.

Herança da resistência aos herbicidas inibidores da ALS em biótipos da planta daninha Euphorbia heterophylla

Os herbicidas inibidores da ALS são os principais produtos aplicados para o controle da planta daninha amendoim-bravo (Euphorbia herterophylla) em lavouras de soja; no entanto, já foram identificados biótipos desta planta daninha resistentes a estes herbicidas no Brasil. O objetivo desta pesquisa foi estudar a herança, o número de genes que conferem a resistência e o grau de resistência dos biótipos homozigotos e heterozigotos resistentes. Foram realizados cruzamentos recíprocos entre os genitores suscetíveis e resistentes para obtenção de sementes F1 e, posteriormente, realizaram-se os retrocruzamentos (RC) com os genitores resistente (R) e suscetível (S). Plantas F1 foram autofecundadas artificialmente para obtenção da geração F2. As plantas F1, F2, RCr e RCs e dos genitores foram tratadas com o herbicida imazethapyr (150 g ha-1). Para avaliar o grau de resistência, plantas F1 e os genitores resistente e suscetível foram tratados com as doses de imazethapyr de 0, 100, 200, 400, 800 e 1.600 g ha-1. As plantas F1 mostraram-se totalmente resistentes ao herbicida, demonstrando que a resistência é nuclear e dominante. As plantas F2 apresentaram alta probabilidade para segregação 3:1, indicando que a resistência é codificada por um gene dominante. A aplicação de doses de imazethapyr sobre as plantas F1 demonstrou que os biótipos homozigotos resistentes e os heterozigotos apresentam o mesmo grau de resistência para doses de até 1.600 g ha-1 deste herbicida. A resistência é codificada por um gene dominante nuclear com dominância completa.

Narrow Row Spacing Does Not Affect Lima Bean Yield or Management of Weeds and Other Pests

Potential increases in the yield of agronomic crops through enhanced light interception have led many growers to consider using narrow rows in lima bean (Phaseolus lunatus L.). However, no information is available on how narrow row spacing affects weed management or fits into an integrated pest management strategy. To address this, field studies were conducted in Delaware and Maryland in 1996 and 1997 to evaluate the effects of row spacing (38 vs. 76 cm) on weed control, and on yield and quality of lima bean. Weed management inputs were also evaluated with labeled or reduced pre-emergence rates of metolachlor plus imazethapyr applied broadcast or banded. Only 76-cm rows were cultivated according to the standard practice for this production system. In general, row spacing, herbicide rate, and herbicide application method had no effect on lima bean biomass or yield, on weed density, control, or biomass production, or on economic return. However, weed control consistency was improved when wide rows were used, even with reduced herbicide rates, possibly because of cultivation. Using reduced herbicide rates and band applications resulted in 84% less herbicide applied without affecting weed control. Chemical names used: 3-(1-methylethyl)-(1H)-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxide (bentazon); 2-[4,5-dihydro-4-methyl-4-(1-methylethyl-4-(1-methylethyl)-5-oxo-1Himidazol-2-yl]-5-ethyl-3-pyridinecarboxylic acid (imazethapyr); 2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide (metolachlor); 2-[1-(ethoxyimino)butyl]-5-[2-ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one (sethoxydim).

Effects of Imazethapyr and Pendimethalin on Lentil (Lens culinaris), Pea (Pisum sativum), and a Subsequent Winter Wheat (Triticum aestivum) Crop1

Lentil and pea are two important crops grown in rotation with winter wheat in the Palouse region of Idaho and Washington. Imazethapyr plus pendimethalin often is used to control weeds in lentil and pea, but the effects of these herbicides on these crops and the subsequently planted winter wheat crop are not well known. The component and combined effects of several rates of imazethapyr and pendimethalin on growth and yield of lentil and pea and the subsequently planted winter wheat crop were measured in 1997 and 1998 field experiments. No herbicide treatment reduced lentil or pea biomass or seed yield compared with the untreated control. Wheat biomass was reduced 35 to 51%, and grain yield was reduced 11 to 17% in all plots treated with 2,240 g/ha pendimethalin at the lentil hilltop site. Imazethapyr at 106 g/ha plus 1,120 g/ha pendimethalin also reduced wheat biomass 24% at the lentil hilltop site. Wheat was not injured at other sites or by other treatments at the lentil hilltop site.Nomenclature: Imazethapyr; pendimethalin; lentil, Lens culinaris L.; pea, Pisum sativum L.; wheat, Triticum aestivum L.Additional index words: Herbicide persistence, rotational crop safety.Abbreviations: DAP, days after planting; DAT, days after treatment; PPI, preplant incorporated.

Integration of Cropping Practices and Herbicides Improves Weed Management in Dry Bean (Phaseolus vulgaris)1

A field study was conducted to determine the combined effects of row spacing, plant density, and herbicides on weed management and dry bean (Phaseolus vulgaris) yield. In weed-free dry bean, a reduction in row spacing from 69 to 23 cm increased yield by 19% and an increase in density from 20 to 50 plants/m2 increased yield by 17%. In the presence of weeds, narrow rows and high plant densities increased dry bean yield, but without herbicides, yields remained low. However, when combined with herbicides, narrow-row and high-density production practices resulted in better weed control and higher dry bean yield than that attained in a wide-row and low-density production system. Herbicide combinations, often at reduced rates, controlled weeds as well or better than the full rate of any individual herbicide. Ethalfluralin applied preplant incorporated followed by reduced rates of imazethapyr or bentazon postemergence (POST) consistently controlled weeds. Imazamox exhibited the potential to provide a total POST weed control option in dry bean production. Information gained in this study will be used to develop improved weed management programs appropriate for either wide- or narrow-row dry bean production systems.Nomenclature: Bentazon, 3-(1-methylethyl)-(1H)-2,1,3-benzothiadiazin-4(3H)-one 2,2- dioxide; ethalfluralin, N-ethyl-N-(2-methyl-2-propenyl)-2,6-dinitro-4-(trifluoromethyl)benzenamine; imazamox, 2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-(methoxymethyl)-3-pyridinecarboxylic acid; imazethapyr, 2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-ethyl-3-pyridinecarboxylic acid; small red dry bean, Phaseolus vulgaris L. ‘Ember.’Additional index words: Integrated weed management, plant density, row spacing, ECHCG, POLCO, POLSC, SETVI, SOLSA.Abbreviations: PAR, photosynthetically active radiation; POST, postemergence; PPI, preplant incorporated.

Maize cultivars differ in tolerance to imazethapyr

The low tolerance of maize (Zea mays L.) to imazethapyr ((±)-2-[4,5-dihydro-4 methyl-4-(1 -methylethyl)-5-oxo 1H-imidazol-2-yl]-5-ethyl-3-pyridinecarboxylic acid) resulted in extensive crop damage in 1992/93 due to residue carry-over from resistant soybean (Glycine max L.). Pot experiments were conducted to assess the role of cultivar differences in maize tolerance, as well as the potential for synergistic interaction between imazethapyr and a fungicide. A selection of white and yellow maize cultivars were tested. The fungicide captab (3a, 4, 7, 7a- tetrahydro-2-[(trichloromethyl)thio]-1H-isoindole-1, 2(2H)-dione) was investigated in terms of its potential to exacerbate damage caused by imazethapyr to various maize cultivars. White cultivars were significantly more sensitive to imazethapyr than the yellow cultivars, with damage in shoot dry mass ranging from 13 to 63% at the lowest herbicide rate of 5 g ai ha−1. The ED50-values derived from linear regression equations for the relationship between imazethapyr rate and maize damage confirmed the differential tolerance of white and yellow cultivars, and indicated that the sensitivity of all six cultivars of the former type increased in the presence of captab. Except for one of six yellow cultivars, the same tendency was found in this group. The mechanisms involved in these differential plant responses are not known. Their elucidation could introduce flexibility into the safety (waiting) period specified for maize, through rational cultivar selection and promote the breeding of cultivars that are tolerant to the broad spectrum of acetolactate synthase (ALS)-inhibiting herbicides.

Hoary Cress (Cardaria draba) Management with Imazethapyr

Currently, there are no effective herbicides registered for the control of hoary cress (Cardaria draba) in alfalfa (Medicago sativa) or alfalfa/grass mixtures. Due to this lack of control options, field experiments were conducted near St. Ignatius, MT, from 1995 through 1997 to determine the optimum imazethapyr rate and adjuvant combination for hoary cress control in alfalfa/orchardgrass (Dactylis glomerata) pastures. Imazethapyr was applied in the spring to rosette stage hoary cress plants at 33, 67, and 100 g ai/ha with either a nonionic surfactant or methylated seed oil alone or combined with 28% urea ammonium nitrate (UAN) liquid fertilizer. Hoary cress control was poorest when imazethapyr was applied at 33 g/ha, yet provided a minimum of 80% control. Imazethapyr applied at 67 and 100 g/ha provided greater than 90% control during the year of application regardless of surfactant or UAN additions. None of the treatments controlled hoary cress the year following application. Alfalfa yield was unaffected by imazethapyr applications. First-cutting orchardgrass yield decreased as imazethapyr rate increased, but total seasonal forage yield was unaffected.

Spray Adjuvant, Formulation, and Environmental Effects on Synergism from Post-Applied Tank Mixtures of SAN 582H with Fluazifop-P, Imazethapyr, and Sethoxydim

The effect of adding a spray adjuvant to SAN 582H tank mixtures with fluazifop-P, imazethapyr, and sethoxydim was evaluated. SAN 582H synergistically increased broadleaf signalgrass control with reduced rates of all three postemergence (POST) herbicides when no spray adjuvant was used and when crop oil concentrate was added. For example, broadleaf signalgrass control increased from 50% to 83% when SAN 582H was tank-mixed with 52 g ai/ha sethoxydim and crop oil concentrate. In another experiment, several formulations of SAN 582H, including blank solvent-only formulations (no SAN 582H), were evaluated in combination with a reduced rate of sethoxydim to determine the source of synergism from tank mixtures. The SAN 582H molecule, not the carrier solvents in formulated product, was determined to be the source of synergism. The synergistic properties of SAN 582H were compared to other chloroacetamides. Synergism from acetochlor was similar to SAN 582H when applied POST with a reduced rate of either fluazifop-P, imazethapyr, or sethoxydim for grass control. Metolachlor also synergistically increased the control of grasses with the POST herbicides; however, metolachlor caused considerable phytotoxicity when applied alone and synergistic interactions were detected less frequently. The efficacy of sethoxydim mixed with SAN 582H was evaluated under different soil moisture conditions. Broadleaf signalgrass control increased from 81% to 93% under dry, moisture-stressed conditions when 210 g/ha sethoxydim was tank- mixed with SAN 582H.

Synergism of Grass Weed Control with Postemergence Combinations of SAN 582 and Fluazifop-P, Imazethapyr, or Sethoxydim

Greenhouse and field experiments were conducted to evaluate early postemergence (POST) tank mixtures of SAN 582 with fluazifop-P, imazethapyr, or sethoxydim. In the greenhouse, SAN 582 synergistically improved barnyardgrass, broadleaf signalgrass, and johnsongrass control from imazethapyr and sethoxydim. Half-rates of imazethapyr and sethoxydim tank-mixed with SAN 582 controlled grass weeds as well as full rates of either herbicide applied alone. Grass weed control with imazethapyr increased up to 40% with the addition of SAN 582. In field experiments, SAN 582 increased grass control with imazethapyr to a lesser degree than observed in the greenhouse. In a multispecies study, grass weed control increased up to 15% when SAN 582 was tank-mixed with a reduced rate of imazethapyr, although the full rate of imazethapyr applied POST with or without SAN 582 controlled grass weeds 80% or less. The combination of SAN 582 with sethoxydim was synergistic for barnyardgrass and johnsongrass control in this experiment. When applied POST in soybean, SAN 582 plus fluazifop-P or sethoxydim controlled barnyardgrass throughout the season better than a single POST application of a graminicide.

Weed Control with Reduced Rates of Chlorimuron Plus Metribuzin and Imazethapyr in No-Till Narrow-Row Soybean (Glycine max)

Field studies were conducted at three locations in 1993 and 1994 to evaluate weed control and crop response to combinations of glyphosate, metolachlor, 0.5 X and 1 X label rates of chlorimuron plus metribuzin applied prior to planting (PP), and 0.5 X and 1 X label rates of imazethapyr applied early postemergence (EPOST) or postemergence (POST) in no-till narrow-row soybean production. Giant foxtail densities were reduced with sequential PP followed by (fb) EPOST or POST treatments. Large crabgrass was reduced equivalently with all herbicide combinations involving chlorimuron plus metribuzin PP fb imazethapyr. Common cocklebur control was variable but was usually greater with treatments that included imazethapyr. Ivyleaf morningglory densities were not reduced with any herbicide combinations. Sequential PP fb EPOST or POST treatments tended to provide slightly better weed suppression than PP-only treatments, but the difference was rarely significant. Soybean yields with treatments utilizing 0.5 X rates were usually equal to 1 X rates.

Weed control with reduced rates of imazaquin and imazethapyr in no-till narrow-row soybean (Glycine max)

Field studies were conducted at three locations in 1993 and 1994 to evaluate weed control and crop response to metolachlor plus combinations of 0.5 × and 1 × label rates of imazaquin applied preplant and imazethapyr applied early postemergence or postemergence in no-till narrow-row soybean production. Giant foxtail, common ragweed, common cocklebur, and large crabgrass population reductions were greater with sequential preplant metolachlor plus imazaquin followed by early postemergence or postemergence imazethapyr than with preplant metolachlor plus imazaquin or early postemergence/postemergence imazethapyr alone. Ivyleaf morningglory was not effectively controlled by any herbicide program. Pennsylvania smartweed populations were reduced with all herbicide treatments. Soybean yields with treatments utilizing 0.5 × rates were usually equal to 1 × rates if imazethapyr was applied early postemergence or postemergence. Net income with reduced herbicide rates was equal to full-label rates and provided no greater risk to net income.

Soil Persistence of Imazethapyr and Detection Using a Sensitive Bioassay Technique

The effects of imazethapyr on root growth of vegetable crops were compared between a lab bioassay and vegetative crop growth in field plots. In the bioassay study, at 5 d after seeding, the lowest imazethapyr rates to cause significant root length reduction of table beets (Beta vulgaris L. 'Early Wonder'), cabbage (Brassica oleracea L. 'Bravo'), cucumber (Cucumis sativus L. 'Marketmore 76'), and sorghum [Sorghum bicolor (L.) Moench 'Corral'] were 0.5, 2.5, 10, and 25 ppb (w/w soil), respectively. In the field, where crops were planted 5 d after preplant incorporated (PPI) herbicide application, the lowest rates to cause dry shoot weight reduction of table beets, spinach (Spinacia oleracea L. 'Seven-R'), cabbage, cucumber, and sorghum were 0.005, 0.01, 0.025, 0.025, and 0.025 lb ai/acre, respectively. These rates are approximately equivalent to concentrations of 5 and 10 ppb respectively for table beets and spinach; and 25 ppb for cabbage, cucumber, or sorghum, based on a 4-in. topsoil weighing 1 × 10 6 lb. However, cabbage planted into treated plots 4 and 12 mo after herbicide application was not injured. In field soil samples collected at monthly intervals starting 45 d after herbicide application (DAHA), imazethapyr at 0.0025 to 0.05 lb ai/acre was not detected beyond 75 DAHA using beet root growth as herbicide indicator. However, at 0.10 lb ai/acre the herbicide persisted until 105 DAHA but was not detected 135 DAHA.

Acetolactate Synthase-Resistant and -Susceptible Corn (Zea mays) Response to Imazethapyr, Imazaquin, Chlorimuron, and CGA-152005

Field experiments were conducted from 1993 to 1995 to evaluate the response of ALS-resistant and ALS-susceptible corn to imazethapyr, imazaquin, chlorimuron, and CGA-152005 at label and twice label rates applied preplant incorporated (PPI) and postemergence (POST). The susceptible hybrid (P3245) and the resistant hybrid (P3245IR) grain yield were not different where weeds were removed by hand-weeding alone. Alachlor plus atrazine alone plus hand-weeding reduced grain yield by 5% compared to hand-weeding only. Imazethapyr applied PPI and POST reduced height of the susceptible hybrid 11 and 6%, respectively, averaged across imazethapyr rates. Imazaquin reduced the final height of the susceptible hybrid by 30 to 75% and reduced corn density by 62 to 78%. Chlorimuron applied PPI reduced final corn height of the susceptible hybrid by 26 to 36% and density by 27%. Chlorimuron applied PPI and imazaquin applied PPI and POST reduced grain yield of the susceptible hybrid. Imazethapyr did not reduce grain yield of the susceptible hybrid, regardless of application timing or rate. Imazethapyr, imazaquin, and chlorimuron did not reduce height, density, or grain yield of the resistant hybrid, regardless of application timing or rate. CGA-152005 applied PPI reduced grain yield of the susceptible and resistant hybrid. CGA-152005 applied POST did not reduce grain yield, regardless of hybrid or rate.

Imazethapyr, Alone or with Other Herbicides for Weed Control During Alfalfa (Medicago sativa) Establishment

During the establishment of alfalfa, POST applications of imazethapyr at 50 g/ha, following PPI applications of trifluralin at 1,100 g/ha, consistently provided ≥ 80% control of wild oat, volunteer canola, and shepherd's-purse with no crop injury. POST applications of the same rate of imazethapyr without PPI applications of trifluralin provided similar volunteer canola and shepherd's-purse control, but ≥ 80% wild oat control was achieved only 25% of the time. Imazethapyr, alone or following trifluralin, did not control common groundsel. Broadleaf weed control from imazethapyr at 50 g/ha tank mixed with fluazifop-P at 125 g/ha or sethoxydim at 200 g/ha was not reduced compared to imazethapyr alone. However, the probability of controlling wild oat by ≥ 90% was reduced from 83% to 0 when the same rate of imazethapyr was tank mixed with fluazifop-P, compared to fluazifop-P alone. Tank mixtures of imazethapyr at 50 g/ha and sethoxydim controlled wild oat only slightly less than sethoxydim alone. First cut and total herbage yields, obtained 1 yr after alfalfa establishment, increased where treatments effectively controlled broadleaf weeds and wild oat, compared to check plots. Second cut herbage and seed yields, also obtained 1 yr after alfalfa establishment, were not affected by any of the treatments.

Economics and Efficacy of Postemergence Spurred Anoda (Anoda cristata) Control in Pinto Beans (Phaseolus vulgaris)

Spurred anoda is an increasing weed problem in Colorado pinto bean production. This research was designed to evaluate reduced rates of bentazon and imazethapyr applied at various times for spurred anoda control and profitability. In 1993, spurred anoda control with early applications of either bentazon or imazethapyr was superior to late applications. Bentazon provided better spurred anoda control than imazethapyr in June. Spurred anoda control in 1993 was better than in 1994 due to late-emerging weeds and herbicide treatments not providing residual control. In 1994, bentazon controlled spurred anoda better than imazethapyr 1 and 3 wk after late POST, but no treatment provided commercially acceptable full-season control. The number of treatments with gross margins greater than the untreated check increased as pinto bean prices increased, but ranking of gross margin did not change due to pinto bean prices.

Weed Control by Spring Cover Crops and Imazethapyr in No-till Southern Pea (Vigna unguiculata)

Studies were conducted at the Vegetable Substation in Kibler, AR, in 1992 and 1993, in the same plots, to evaluate weed suppression by spring-seeded cover crops and to determine the effects of cover crop and imazethapyr on no-till southern pea. A plot without cover, conventionally tilled before planting southern pea, served as control. Weed control treatments, applied as subplots in each cover crop, included a weedy check, handweeded check, and half and full rates of imazethapyr (0.035 and 0.07 kg/ha) followed by sethoxydim (0.22 kg/ha). Biomass of Palmer amaranth 6 WAR without herbicides, was less in Italian ryegrass and sorghum-sudangrass residues than in oat residue and no cover crop. Over the years, Palmer amaranth density increased 333% without cover crops and 28% with cover crops. Rice flatsedge density increased four to five times in oat and sorghum-sudangrass residues but remained the same in Italian ryegrass residue. In general, Italian ryegrass residue suppressed the most weeds. Oat residue was least suppressive. Italian ryegrass and sorghum-sudangrass also reduced southern pea stand. Regardless of cover crop and year, half and full rates of imazethapyr followed by sethoxydim equally reduced density of Palmer amaranth, goosegrass, large crabgrass, southwestern cupgrass, and rice flatsedge compared with the untreated check. Residual control of Palmer amaranth by imazethapyr was higher at the full rate than the reduced rate, regardless of cover crop. Half rate of imazethapyr followed by sethoxydim controlled 94 to 100% of Palmer amaranth, rice flatsedge, large crabgrass, and southwestern cupgrass late in the season, regardless of cover crop in 1992 and 1993. Southern pea yield in untilled plots with cover crops was two to three times lower than yield in plots with preplant tillage and no cover crops mostly because of reduction in crop stand in the presence of cover crops.

Thifensulfuron and Imazethapyr Interaction at the ALS Enzyme in Sulfonylurea-Tolerant Soybean (Glycine max)

Greenhouse and laboratory experiments were conducted to determine if the synergistic injury to sulfonylurea-tolerant soybean (STS soybean) caused by applications of thifensulfuron plus imazethapyr involves an interaction between the herbicides at the ALS enzyme. In vitro ALS from STS soybean (Asgrow 3200) was 10 times more tolerant to thifensulfuron than ALS from non-STS soybean (Williams 82). In vivo ALS was 30% of maximum activity 6 HAT in STS soybean treated with 4.4 g ha−1thifensulfuron, but 0% of maximum in similarly treated non-STS soybean. Increasing thifensulfuron from 4.4 to 141 g ha−1did not increase in vivo ALS inhibition in STS soybean, indicating a highly resistant ALS isozyme. Both cultivars responded similarly to imazethapyr in terms of visual injury, in vitro ALS inhibition, and in vivo ALS inhibition. Soybean injury and in vivo ALS inhibition increased with imazethapyr rate in both cultivars. The relationship between imazethapyr inhibition of ALS in vivo and soybean injury was curvilinear. In vitro ALS assays revealed no synergistic interaction between thifensulfuron and imazethapyr at the enzyme level. In vivo ALS activity was reduced to 7 and 10% of controls 24 and 48 HAT by the tank mixture compared to 56 and 84% for thifensulfuron alone and 20 and 28% for imazethapyr alone. These data show that the synergistic increase in soybean injury caused by tank mixing thifensulfuron with imazethapyr results from the cumulative inhibition of ALS, not a synergistic inhibition of the enzyme.

Response of Eastern Black Nightshade (Solanum ptycanthum) to Low Rates of Imazethapyr and Metolachlor

Eastern black nightshade is a problem weed in soybeans primarily due to interference with harvesting operations and reductions in crop quality. Field and greenhouse studies were conducted in 1990 and 1991 at Harrow, Ontario, to determine whether sublethal rates of imazethapyr and metolachlor could suppress growth and berry production by eastern black nightshade. In the greenhouse, plants treated with metolachlor PRE at 0.8 kg ai ha−1or imazethapyr POST at 30 g ai ha−1(1/3 to 1/2 the labelled rates) produced significantly fewer berries in the first flower cluster, with fewer seeds per berry, than untreated plants. When a surfactant was added to the imazethapyr, almost all flower buds in the first cluster were aborted. Treated plants recovered from the damage, however, and did not differ significantly from control plants in dry weight or berry production 110 DAP. In the field, application of 50 g ai ha−1imazethapyr POST (2/3 of the lowest labelled rate), with or without a surfactant, resulted in delayed flowering, abortion of most of the flower buds in the first cluster, and greatly reduced growth and total berry production between soybean leaf abscision and harvest. Application of metolachlor + metribuzin PPI in the field at 2/3 of the labelled rate (1.6 + 0.25 kg ai ha−1) was not sufficient to reduce nightshade growth and berry production after soybean leaf abscision. Sublethal rates of imazethapyr and metolachlor did not reduce seed viability in either the greenhouse or the field.