This study investigated the impact of tool-work interface temperature during the turning process of a cylindrical workpiece made of 6061 aluminum alloy. The experiment utilized four different cutting fluids: palm oil-based cutting fluid (POBCF), neem seed oil-based cutting fluid (NOBCF), orange seed oil-based cutting fluid (OSOBCF), and mineral oil-based cutting fluid (MOBCF) using uncoated carbide cutting tool of grade H13A. Various machining parameters were considered, including spindle speed (180, 250, 355, 500 rpm), feed rate (0.105, 0.116, 1.4, 1.6 mm/rev), and depth of cut (0.5, 1.0, 1.5, 2.0 mm). The experimental design followed the Taguchi specified L16 (43) orthogonal array and was conducted on a Lathe Machine XL 400. To measure the tool-work interface temperature, an infrared thermometer (KM 690) was employed during the aluminum alloy machining process. Subsequently, a mathematical model for the tool-work interface temperature values was developed through regression analysis using Minitab 16. The significance of the chosen machining parameters and their respective levels on the tool-work interface temperature was determined using analysis of variance (ANOVA) and F-test. The findings indicated that machining under orange seed oil-based cutting fluid (OSOBCF) conditions resulted in a 9.02%, 16.4%, and 21.7% lower temperature at the tool-work interface compared to palm oil-based cutting fluid (POBCF), neem seed oil-based cutting fluid (NOBCF), and conventional oil-based cutting fluid (MOBCF), respectively. This suggests a potential for producing higher-quality products under orange seed oil cutting fluid conditions compared to other wet conditions.