Biogas Production Rate Research Articles

Efficient methane production from anaerobic co-digestion of poultry litter with wheat straw in sequencing batch reactor: Effects of carbon-to-nitrogen ratio, total solids, and hydraulic retention time

This study performed co-digestion of poultry litter (PL) with wheat straw in the daily anaerobic sequencing batch reactor considering operation parameters of carbon-to-nitrogen ratio (C/N, 11.6 to 28.4), total solids (TS, 2.6 to 9.4%), and hydraulic retention time (HRT, 7.6 to 24.4d). The inoculum with a diverse microbial community structure included 2% of methanogens (Methanosaeta) was chosen. Experimental performance by central composite design showed continuous methane production with the highest biogas production rate (BPR) obtained at C/N = 20, TS = 6%, and HRT = 7.6d, being (1.18 ± 0.14 L/LR/d). A significant modified quadratic model (p < 0.0001) for predicting BPR was built (R2 = 0.9724). The operation parameters and process stability both affected the release of nitrogen, phosphorus, and magnesium in the effluent. The results provided new support for the novel reactor operations for efficient bioenergy production from PL and agricultural wastes.

Kinetic modeling and interrelationship aspects of biogas production from waste activated sludge solubilized by enzymatic and thermal pre-treatment

Monte Carlo Simulation (MCS) and kinetic models were applied using data from the biogas production test of waste activated sludge (WAS) pre-treated samples. Indeed, eleven different pre-treatments were applied, combining enzymatic and thermals technologies of sludge solubilization. The main study was proposed to understand the kinetic dynamics of the anaerobic digestion (AD) process and its biogas production potential as a renewable energy source, optimized by distinct solubilization techniques that can be applied to enhance biogas production. As a result, most of the evaluated scenarios significantly improved the biogas production, when compared to the control scenario. Among those, the scenario with thermal solubilization and enzymatic solubilization associated in series, obtained the greatest biogas production potential, reaching 291 ± 3 NmL.gVSS-1, corresponding to a production increase of about 71%. The maximum rate of biogas production obtained was 33.5 ± 0.7 NmL.gVSS-1.day−1. The average lag phase duration (λ) was 0.56 days, and the hydrolysis coefficient (kh) varied between the minimum value of 0.08 ± 0.002 and the maximum of 0.15 ± 0.003 days−1. The two-way ANOVA indicated that there is an interrelationship between the solubilization techniques applied. The confidence interval obtained through MCS allowed us to conclude that the adjustments of the models and the obtention of its parameters to all the scenarios, with exception of the control one, do not have significant differences between them. Therefore, more than a single application of kinetic models to biogas production test results, the statistical approaches supported to elucidate and measure the influence of solubilization techniques in the bioconversion of WAS to biogas as renewable energy source.

Enhancement of biogas production rate from bioplastics by alkaline pretreatment

The effect of alkali-based pretreatment on the methanization of bioplastics was investigated. The tested bioplastics included PHB [poly(3-hydroxybutyrate)], PHBH [poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)], PHBV [poly(3-hydroxybutyrate-co-3-hydroxyvalerate], PLA (polylactic acid), and a PLA/PCL [poly(caprolactone)] 80/20 blend. Prior to methanization tests, the powdered polymers (500–1000 μm) at a concentration of 50 g/L were subjected to alkaline pretreatment using NaOH 1 M for PLA and PLA/PCL, and NaOH 2 M for PHB-based materials. Following 7 days of pretreatment, the amount of solubilized carbon for PLA and its blend accounted for 92–98% of the total initial carbon, while lower carbon recoveries were recorded for most PHB-based materials (80–93%), as revealed by dissolved total organic carbon analysis. The pretreated bioplastics were then tested for biogas production by means of mesophilic biochemical methane potential tests. Compared to unpretreated PHBs, methanization rates of pretreated PHBs were accelerated by a factor of 2.7 to 9.1 with comparable (430 NmL CH4/g material feed) or slightly lower (15% in the case of PHBH) methane yields, despite featuring a 1.4–2.3 times longer lag phases. Both materials, PLA and the PLA/PCL blend, were only extensively digested when pretreated, yielding about 360–380 NmL CH4 per gram of material fed. Unpretreated PLA-based materials showed nearly zero methanization under the timeframe and experimental conditions tested. Overall, the results suggested that alkaline pretreatment can help to enhance the methanization kinetics of bioplastics.

Integration of mesophilic biogas plant in the animal slaughter process under real limitations: Techno-economic evaluation of a colombian bovine slaughterhouse.

Anaerobic digestion (AD) has been a widely tested alternative for the management and valorization of wastewater from the animal slaughter process. However, the integration of AD in slaughterhouses depends on technical and economic aspects. In Colombian slaughterhouses AD integration is limited by the availability of land. In the present study, a techno-economic evaluation of the AD of offal wastewater (OWW) stream in a laboratory scale mesophilic tubular digester was carried out. The digester was operated at organic loading rates (OLR) of 0.28, 0.50, 1.0, 1.5 and 2.0kg VS/m3 d. Boilers and a CHP (combined heat and power) system were considered for energy integration of biogas. For the economic study, the cost structure of a Colombian slaughterhouse was considered. The AD of OWW at 2.0kg VS/m3 d OLR was unstable with risk of inhibition. Increasing the OLR from 0.28 to 1.5kg VS/m3 d caused a reduction in the specific biogas production (SBP) from 0.474 to 0.069m3/kg VS However, the biogas production rate (BPR) remained constant at around 0.105m3/m3dig d for OLRs>0.28kg VSm3 d. Therefore, OWW anaerobic digestion in low-cost mesophilic biogas plants is technically feasible with OLRs between 0.28 and 1.5kg VS/m3 d. The implementation of boilers is economically favorable for OLR≥1.0kg VS/m3 d. Nevertheless, feasibility is very sensitive to variations in the cost structure. The implementation of CHP was feasible in the range of OLRs evaluated and its viability is not affected by changes in assumed costs.

Combinatorial Effect of Process Parameters on the Rate of Biogas Production and Rate of Substrate Degradation Following Anaerobic Digestion of Food Waste and Rumen Content

In this study, the combinatorial effect of process parameters on the rate of biogas production and rate of substrate degradation following anaerobic digestion of food waste and rumen content using mixture design (Combined I-optimal) within the Design Expert (version 13) environment. Results showed that the rate of biogas production, rate of bio-methane production and rate of substrate degradation inside the 100 bio-digesters ranged from 0 to 38.04 L/Kg VS, 0 to 23.14 L/Kg VS and 0 to 79.20 %, respectively. The highest rate of biogas production (38.04 L/Kg VS), highest rate of bio-methane production (23.14 L/Kg VS) and highest rate of substrate degradation (79.20 %) were observed in bio-digester 57 at food waste (0.30 kg), rumen content (0.30 kg), water content (0.40 kg), temperature (34.0oC), pH (9.0), number bio-digester of agitation per day (4 time/day) and retention time (32 days), respectively. The rate of biogas/bio-methane production and rate of substrate degradation can vary, with varying process factors/parameters in anaerobic digestion processes. Bio-digesters with anaerobic co-digestion of the food waste and rumen content appeared to be significantly more productive in terms of biogas/bio-methane production rate and substrate degradation rate compared to the bio-digesters with anaerobic mono-digestion of either food waste or rumen content regardless of the presence other process factors/parameters within the boundaries of this investigation.

Improvement of Asterarcys quadricellulare biomass solubilization and subsequent biogas production via pretreatment approaches: structural changes and kinetic modeling evaluation.

This study investigated the effect of enzymatic and hydrothermal pretreatment approaches on the solubilization of organic matter, structure, and biogas yield from microalgal biomass. The soluble chemical oxygen demand (sCOD) concentration increased by 1.21-3.30- and 5.54-6.60-fold compared to control by enzymatic and hydrothermal pretreatments respectively. The hydrothermal pretreatment affected the structural changes in the microalgal biomass markedly; nonetheless, increased enzymatic concentration also had a definite effect on it as determined by qualitative approaches like scanning electron microscopy and Fourier transform infrared spectroscopy. Also, the hydrothermal pretreatment (100°C for 30min) resulted in the highest biogas production potential (P) of 765.37 mLg-1 VS at a maximum biogas production rate (Rm) of 22.66 mLg-1day-1 with a very short lag phase (λ) of 0.07days. The biogas production of pretreated microalgal biomass particularly at higher enzyme dose (20%, 24h) and higher hydrothermal pretreatment temperature (120°C, 30min) showed a significant but weak correlation (R = 0.53) with sCOD, thus demonstrating that the less organic matter was used up for the biogas production. The modified Gompertz model explained the anaerobic digestion of microalgal biomass more accurately and had a better fit to the experimental data comparatively because of the low root mean square error (3.259-16.728), residual sum of squares (78.887-177.025), and Akaike's Information Criterion (38.605-62.853).

Comparison of thermal alkaline pretreatment and zinc acetate-catalyzed methanolysis (MtOH-ZnOAc) for anaerobic digestion of bioplastic waste

The aim of this work was to study the effect of thermal alkaline pretreatment and zinc acetate-catalyzed methanolysis (MtOH-ZnOAc) in biogas production from bioplastic in anaerobic digestion. The pretreated bioplastic with MtOH-ZnOAc performs efficient solubilization and produced 205.7 ± 6.9 mL/g CODadded, which is higher than thermal alkaline degradation. The mesophilic condition produces more than 79% higher biogas compared with the thermophilic condition with the diluted pretreated bioplastic by 30 times. The kinetic study was well fit the experimental data and showed the correlation between cumulative biogas, production rate, and lag phase with mono- and two-stage system in batch fermentation. The two-stage system produced 315.6 ± 7.7 mL/g CODadded which was higher 67.2 ± 2.02 than the mono-stage system. Methanosaetaceae predominates among the Archaea, which are primarily responsible for methanogenesis, showing a contribution to a higher biogas production rate.

Biogas yield using single and two stage anaerobic digestion: An experimental approach

Untreated sewage sludge is one of the biggest problems in India and around the globe. In the current study, we try to figure out an efficient technique that uses sewage sludge (SS) food waste (FW) to generate biogas. In this work, FW and SS are co-digested in single-stage anaerobic digestions (SSAD) and two-stage anaerobic digestions (TSAD) at various processing parameters, such as temperature, carbon-to-nitrogen (C/N) ratio, volatile solid (VS) percentage content, retention time (RT), and pH value. The high-solid digestion of FW and SS in the SSAD process reduces the biogas production rate compared to the TSAD process with 35 days of retention time at mesophilic temperature due to the oily substance from FW accumulating in the scum floating layer in the SSAD process. By altering the mass ratio between FW and SS (based on VS%), the TSAD process could be more effective. This ratio of 60:40 between FW and SS would produce more biogas with maximum efficiency in TSAD compared to the SSAD process. The results highlight an increase in biogas production and VS removal efficiency of the TSAD process 41.90% and 9.8%, respectively, compare to the SSAD process. The cumulative biogas yield in the TSAD process was 1914.8 ml and in the SSAD process was 1349.4 ml when the ratio of FW and SS was taken to be 60:40. In the TSAD process, Methane (CH4) concentrations increased on average from 51% to 75%, due to better substrate hydrolysis and more readily accessible volatile fatty acids in the second stage. The analysis shows the co-digestion approach and TSAD process might work together to overcome the limitations of the conventional SSAD procedure for FW or SS when used separately.

Non-linear Regression Models for Predicting Biogas Yields from Selected Bio-wastes

The benefits of biogas as alternative energy to other fossil fuel sources, due to its renewability, environmentally friendly nature, health benefits, etc., cannot be overemphasized. There are numerous models for predicting biogas production rate from bio-materials, including the modified Gompertz equation. These models are primarily dependent on specific biomass parameters. When any of these parameters, like the slurry volume, changes, another round of experiments must be conducted and curve fitted before biogas yield predictions can be made. This could be time-consuming and costly. Using experimentally published data, simple empirical models can be developed for predicting biogas yields over a range of input parameters. This will eliminate the need for always performing experiments before biogas yield predictions can be made. In light of this, scarce literature provides explicit models for predicting biogas yield over a range of parameters based on published data. This study developed non-linear regression models using published data on parameters that affect biogas yields, like the slurry volume, carbon-to-nitrogen ratio, temperature, total solids, volatile solids, hydraulic retention time, and pH. The data covered seven readily available bio-wastes, including cow dung, cow dung with plant waste, cow dung with poultry dung, poultry dung with grass, pig dung, and plant wastes. On validation of the models, the results showed that the models had a relatively low standard error of estimates, Akaike information criterion, Schwarz criterion, and Hannan-Quinn information criterion. Furthermore, the coefficients of determination, R2, were between 94.62 and 98.93%. The percentage average absolute deviation (% AAD) for each model was less than 7 %. The non-linear models were found to adequately predict the biogas yields within the limits of the available data set.

Preliminary Observation of Biogas Production from a Mixture of Cattle Manure and Bagasse Residue in Different Composition Variations

The need of renewable energy is paramount important as it is expected to replace fossil energy. One of renewable energy commonly used for rural area is biomass-based energy. Biogas is a biomass-based energy where organic materials are converted to methane gas via anaerobic digestion process. The limitations of mono-feedstock biogas are instability digestion process, low yield biogas produced and require readjusting C/N ratio, therefore co-digestion process was proposed to overcome these problems. This study aims to investigate the feasibility of anaerobic co-digestion of a mixture of cattle manure and bagasse residue in different weight ratio combinations. Biogas was generated by anaerobic digestion using a mixed substrate composed of a combination of weight ratios of bagasse:cattle manure (1:5, 1:2, 1:1, and 3:1). The kinetic analysis was evaluated by fitting Gompertz and Logistic model to experimental data of cumulative biogas. The result showed that the combination of 1:5 ratio of bagasse waste to cattle manure obtained the best biogas yield with cumulative biogas at 31,000 mL. The kinetic model of Gompertz and Logistic were able to predict the maximum cumulative biogas at ratio of 1:5 (cattle: bagasse) at 31,157.66 mL and 30,112.12 mL, respectively. The other predictions of kinetic parameters were maximum biogas production rate (Rm)= 1,720.45 mL/day and 1,652.31 mL/day for Gompertz and Logistic model, respectively. Lag periods were obtained at 2.403 day and 2.612 day for Gompertz and Logistic model, respectively. The potential power generation of 338.71 Watt has been estimated from biogas. This research has proven a positive feasibility of co-digestion of two feed-stocks (cattle manure and bagasse) for biogas production.

Impact of municipal solid waste landfill leachate on biogas production rate

Landfills/open dump sites are the final disposal facilities for municipal solid waste (MSW). These sites undergo continuous process of biochemical reactions and anaerobic degradation, which make them prone to generation of landfill gas (LFG) and leachate. Worldwide, the quantitative and qualitative assessment for leachate treatment and management has been a growing concern. The present study investigated the physico-chemical characteristics and heavy metal parameters for fresh, 3-month, 6-month and 3-year old landfill leachate samples. The total dissolved solids (13280 mg/l), alkalinity (13000 mg/l), chemical oxygen demand (42000 mg/l) and total organic carbon (16500 mg/l) was found to be maximum in 3-year old leachate sample. While, the 3 and 6-month old leachate samples had maximum heavy metal concentration. The attempt was also made to identify the key parameters responsible to enhance biogas production yield from different ages of MSW. The substrate combinations of MSW and 3-year old leachate samples was prepared at varying proportion. The study was performed in three cycles and the volume of leachate diffused in each cycle was kept constant. The control samples with no leachate diffusion was also prepared to compare the percentage increase in biogas production rate. It was found that the cumulative methane (CH4) production from fresh (358 ml/g) and 3-month old MSW (273 ml/g) was maximum, and the overall percentage increase was 43% and 32%. It was also conclusive that the excess leachate diffusion of >15 ml results in low calcination behaviour and CH4 production rate. The response surface methodology was used to correlate and validate independent input variables (volatile solids, C/N ratio and leachate concentration) responsible for maximum CH4 yield.

A Novel Method Using Two-stage Anaerobic Digestion Integrated with Greenhouse Solar Dryer for Increased Biogas Production

Solar energy is infinite and environmental-friendly energy that is widely used in many fields such as solar cells, solar roofs, solar dryers and so on. However, solar energy has never been used in anaerobic digestion processes for biogas production. The use of solar energy may accelerate the biogas reaction to achieve higher gas production rates. Hence, the purpose of this research was to study a two-stage anaerobic digestion system in combination with a greenhouse solar dryer to increase the rate of biogas production. The study consisted of two parts: the first was to study the temperature variations in greenhouse solar dryers using computational fluid dynamic techniques, and the second was to conduct two-stage anaerobic digestion integrated with the greenhouse solar dryer system for biogas production. The results showed that the average temperature and biogas production rate in the integrated two-stage anaerobic digestion system compared to a conventional system increased by 16.2% and 19.69 %, respectively

Evaluating the Potential of Renewable Energy Sources in a Full-Scale Upflow Anaerobic Sludge Blanket Reactor Treating Municipal Wastewater in Ghana

Wastewater management remains a major challenge in developing countries due to the lack of adequate infrastructure, making the need for economically viable and efficient technologies that can be sustained by emerging economies imperative. The upflow anaerobic sludge blanket (UASB) reactor represents an efficient and low-cost technology that produces by-products from which valuable resources can be recovered. This study assessed the energy recovery potential in the form of electricity from biogas and sludge by-products produced by a full-scale UASB reactor. Biogas production rate and composition were monitored to determine the biogas energy recovery potential. Dehydrated sludge from sludge drying beds was likewise quantified and characterised for its elemental composition, immediate composition, gross calorific value and net calorific value to estimate sludge energy recovery potential. The average daily biogas production was found to be 611 ± 275 Nm3/d, with 65% methane in the biogas output. Average sludge dry matter production was determined to be 358.24 TS kg/d. The net energy recovery potential was estimated to be 534.1 MWh/yr, 36% more than the yearly energy demand (392.7 MWh/yr) of the entire plant. Conservative energy recovery at a UASB-based municipal wastewater treatment facility could serve as a self-supply energy option to support its operations.

The Synergistic Effect of Simultaneous Ultrasound Heating and Disintegration on the Technological Efficiency and Energetic Balance of Anaerobic Digestion of High-Load Slaughter Poultry Sewage

Regulations in force urge for thermal pre-treatment of post-slaughter waste prior to its anaerobic digestion. Increased interest in biomethane as a fuel in gas networks or vehicles of road transport forces the need to look for heating methods that are alternative to heat recovery from cogeneration. The goal of this study was to determine the synergistic effect of simultaneous ultrasound heating and disintegration on the technological efficiency and energetic balance of the anaerobic digestion of high-load slaughter poultry wastewater. The highest efficiency of anaerobic digestion was obtained for the ultrasound thermal pre-treatment (60 min, 90 °C, OLR = 2.0 gCOD/dm3). In this experimental variant, the biogas production rate reached 9.0 ± 0.2 cm3/gCOD·h, biogas yield was 492 ± 10 cm3/gCOD, and the biogas produced contained 69.8 ± 1.4% CH4. Given the incurred energy outputs, the highest net energetic efficiencies, i.e., 5.92 ± 0.43 Wh and 5.80 ± 0.42 Wh, were obtained in the variants with the conventional thermal pre-treatment (60 min, 70 °C, OLR = 6.0 gCOD/dm3) and ultrasound thermal pre-treatment (60 min, 70 °C, OLR = 6.0 gCOD/dm3), respectively.

The effect of silage additive on the kinetics of biogas production from lignocellulosic perennial crops

The aim of the study was to assess the effect of silage additive containing heterofermentative lactic acid bacteria (LAB) strain of Lactobacillus buchneri species on ensiling quality, as well as methane yield and the kinetics of biogas production from ensiled perennial energy grasses: Miscanthus × giganteus (miscanthus), Spartina pectinata (cordgrass), Panicum virgatum (switchgrass) and Andropogon gerardii (big bluestem). The listed plants are not commonly used for biogas production, their susceptibility to ensiling is also little known, hence the need to investigate their suitability for these processes. Effective methods for increasing the biogas yield from biomass are still demand, hence the research on the use of LAB for this purpose. After harvesting the grasses were cut and ensiled in barrels with and without (controls) the usage of commercial silage inoculant containing Lactobacillus buchneri LN40177. After 90 days of ensiling obtained silages were analysed in order to compare their chemical composition: organic acids content, the loss of dry matter, the differences in particular fibres composition. The silages were then subjected to methane fermentation using OxiTop® sensors and exposed to air in order to check their aerobic stability. The silages prepared with LAB additive had higher concentration of acetic acid than the control silages prepared without LAB addition, which contributed to increased aerobic stability but had no effect on the methane yield of miscanthus, switchgrass and big bluestem. Using the microbial inoculant during ensiling had beneficial effect in terms of reducing the duration of biogas production process from obtained silages: lag phase was shortened, daily biogas production rate was increased and 90% of biogas was produced in a shorter period of time compared to the control silages from investigated grasses. The modified Gompertz model well reflected the kinetics of biogas production process.

Machine learning for quantile regression of biogas production rates in anaerobic digesters

Anaerobic digestion is a well-established tool at wastewater treatment plants for processing raw sludge; it can also be used to generate renewable energy by harvesting biogas in anaerobic digesters. Operational parameters, such as temperature, are usually set by plant operators according to expert knowledge. To completely utilize the potential of operational management, in this study, we calibrated a novel Temporal Fusion Transformer based on six years of life-scale time series data together with categorical features such as public holidays. The model design allows for the interpretability of the output in contrast to traditional data-driven techniques, using multi-head attention. In addition to forecasting the median biogas production rates for the following seven days, our model also yields quantiles, making it less prone to strong fluctuations. We used three well-known statistical techniques as benchmarks. The mean absolute percentage error of our forecasting approach is below 8 %.

Biogas production from sewage sludge by anaerobic digestion

Some plant residues with digested sewage sludge was used for biogas production at 35o C, and a retention time of 30 days; has been shown that possibility of producing biogas from primary sludge, with daily production rate about 77.41 - 95.52 cm3 biogas . day-1, and the average yield production between 89.22 - 113.88 cm3 biogas per g volatile solids added. And that the results of the gas chromatography analysis, show the presence of a acceptance percentage of methane within the contents of biogas between 52.6 - 59.2%. with surpass of wheat straw media over other plant residues media in biogas production and methane content rate. The study reached to that anaerobic digestion process with batch culture efficient in organic treatment presence in swage sludge with plant residues with good quantity of biogas (methane) production.

Promoting the Circular Economy on an Island: Anaerobic Co-Digestion of Local Organic Substrates as a Possible Renewable Energy Source

The local waste co-digestion is an interesting option to tackle in reduced and isolated areas like the islands. The islands have limited territory and scarce fuel production. Moreover, organic waste can create serious environmental problems in soil, water and air. Anaerobic co-digestion (AcoD) is a technology fulfilling the concept of waste-to-energy (WtE) based on local resources. The valorisation of organic waste through AcoD on an island would prevent environmental impacts, while being a source of renewable energy. In this study, cow manure (outdoor and indoor), pig slurry, bird manure, kitchen waste, sewage sludge and oily lacteous waste produced on Island Terceira (Portugal) were tested in mesophilic -35 °C- Biochemical Methane Potential (BMP) co-digestion assays. The goals were to analyse the recalcitrant and high potential produced waste and to estimate the energetic supply source on the island. The cow manure and pig slurry were used as inocula and specific methanogenic activities (SMAs) were carried out. The results showed that both substrates have a significant methanogenic activity-SMA 0.11 g-COD/(g-VSS.d) and 0.085 g-COD/(g-VSS.d), respectively. All the studied combinations were feasible in AcoD, showing TS removals in the range of 19-37%; COD removals in the range 67-78% and specific methane yields from 0.14 to 0.22 L/gCOD removed, but some differences were found. The modified Gompertz model fitted the AcoD assays (R2 0.982-0.998). The maximum biogas production rate, Rmax. was highest in the AcoD of Cow+Pig+Oily and in the Cow+Pig+Sludge with 0.017 and 0.014 L/g-VSadded.day, respectively, and the lowest in Cow+Pig+Bird with 0.010 L/g-VSadded. In our AcoD studies, the bird manure limited the performance of the process, since it was recalcitrant to anaerobic degradation. On the other hand, the oily lacteous waste showed a great potential in the anaerobic digestion. The estimated biogas production, from the best-studied condition, could cover the 11.4% of the energy supply of the inhabitants. These preliminary results would prevent the environmental impact of organic waste on the island and promote the use of local waste in a circular economy scenario.

Performance evaluation and energy potential analysis of anaerobic membrane bioreactor (AnMBR) in the treatment of simulated milk wastewater

An anaerobic membrane bioreactor (AnMBR) was employed as primary treatment unit for anaerobic treatment of simulated wastewater to produce high effluent quality. A lab scale hollow fiber membrane was used to scrutinize the performance of AnMBR as a potential treatment system for simulated milk wastewater and analyze its energy recovery potential. The 15 L bioreactor was operated continuously at mesophilic conditions (35 °C) with a pH constant of 7.0. The membrane flux was in the range of 9.6–12.6 L/m2. h. The different organic loading rates (OLRs) of 1.61, 3.28, 5.01, and 8.38 g-COD/L/d, of simulated milk wastewater, were fed to the reactor and the biogas production rate was analyzed, respectively. The results revealed that the COD removal efficiencies of 99.54 ± 0.001% were achieved at the OLR of 5.01 gCOD/L/d. The highest methane yield was found to be at OLR of 1.61 gCOD/L/d at HRT of 30 d with the value of 0.33 ± 0.01 L-CH4/gCOD. Moreover, based on the analysis of energy balance in the AnMBR system, it was found that energy is positive at all the given HRTs. The net energy production (NEP) ranged from 2.594 to 3.268 kJ/gCOD, with a maximum NEP value of 3.268 kJ/gCOD at HRT 10 d HRT. Bioenergy recovery with the maximum energy ratio, of 4.237, was achieved with an HRT of 5 d. The study suggests a sizable energy saving with the anaerobic membrane process.

EFFECT OF CO-DIGESTING PRETREATED CHICKEN - GOAT AND UNTREATED COW MANURE ON BIOGAS PRODUCTION IN FIXED DOME LABORATORY DIGESTER

The effect of hydro, mechanical and thermal pretreatment of chicken and goat manure on biogas production wasdone using a 0.15m3 laboratory scale batch digester. The feedstocks were subjected to 6, 12 and 18 hours ofsoaking, 2 mm, 3 mm and 4 mm of mechanical mincing, and 60 ̊C, 80 ̊C and 100 ̊C of heating. Pretreated feedstocks were co-digested with untreated cow manure from an extensive dairy rearing system. Experiments weredone at 8 % substrate total solids and a constant temperature of 35°C. Averagely, mechanical pretreatmentresulted in the highest increase in mean biogas production rate with 11.11%, over the co-digestion control (0.54m3/m3d), followed by thermal and hydro by 5.56% and1.85%. Maximal increase in production for eachpretreatment was at 6 hour soaking time (9.30%), 3 mm effective feed stock particle sizes (18.52%) and 80 ̊C ofheating (14.81%). Co-digestion increased mean biogas production rate over mono-digestion by 68.97%(chicken), 81.84% (goat) and 8.8% (cow manure). Superior outer cell wall and cover disruption of feed stocksfor easy hydrolysis advantaged mechanical pretreatment.