Rat Suprachiasmatic Nucleus Neurons Research Articles

Membrane properties of rat suprachiasmatic nucleus neurons receiving optic nerve input.

1. The electrophysiological properties of suprachiasmatic nucleus (SCN) neurons (n = 33) receiving optic nerve input were studied with intracellular recordings in rat hypothalamic slices maintained in vitro. Our major goal was to provide baseline data concerning the intrinsic membrane properties of these neurons and to test the hypothesis that the neurons are homogeneous electrophysiologically. 2. Action potentials were short in duration and followed by a pronounced hyperpolarizing after-potential. Spike amplitude (58.2 +/- 1.1 mV, mean +/- S.E.M.; measured from threshold), spike duration (0.83 +/- 0.03 ms; measured at half amplitude) and hyperpolarizing after-potential amplitude (23.9 +/- 1.0 mV; measured from threshold) appeared unimodally distributed and did not co-vary. 3. Intracellular injection of depolarizing current pulses evoked spike trains, and spike inactivation, spike broadening and frequency accommodation were always present. An after-hyperpolarization followed the spike train in all but one neuron. 4. Membrane time constant ranged from 7.5 to 21.1 ms (11.4 +/- 0.7 ms, n = 27), and its distribution appeared to be unimodal with the peak at approximately 10 ms. Input resistance ranged from 105 to 626 M omega (301 +/- 23 M omega, n = 33); the distribution also appeared unimodal with its peak at approximately 250 M omega. 5. A subpopulation (16 of 33, 48%) of the neurons exhibited slight (6-29%) time-dependent inward rectification in their voltage responses to hyperpolarizing current injection. Of the neurons lacking the time-dependent rectification, some (n = 5) exhibited time-independent inward rectification of 6-20% and others showed no (or < 3%) such rectification. The degree of inward rectification was correlated with neuronal excitability (r = 0.60, P < 0.002; assessed by measuring the steepness of the primary slope of the frequency-current plot) and with the spontaneous firing rate (r = 0.49, P < 0.007). Furthermore, the neurons with > 6% inward rectification (neurons with time-dependent and -independent rectification were combined) were more excitable (362 +/- 43 Hz/nA (n = 15) vs. 221 +/- 37 Hz/nA (n = 9), P < 0.05) and had a higher spontaneous firing rate (11.1 +/- 1.9 Hz (n = 19) vs. 3.9 +/- 1.5 Hz (n = 11), P < 0.02) than the neurons with no or negligible (i.e. < 3%) inward rectification. The two groups, however, were not significantly different in membrane time constant and input resistance. 6. When adequately hyperpolarized, twelve of seventeen (71%) neurons generated small low-threshold spike (LTS) potentials in response to depolarizing current pulses.(ABSTRACT TRUNCATED AT 400 WORDS)

Role of NMDA and non-NMDA receptors in excitatory postsynaptic potentials in rat suprachiasmatic nucleus neurons.

Role of NMDA and non-NMDA receptors in excitatory postsynaptic potentials in rat suprachiasmatic nucleus neurons.

Inward rectification and low threshold calcium spike in rat suprachiasmatic nucleus neurons

Inward rectification and low threshold calcium spike in rat suprachiasmatic nucleus neurons

Effects of histamine on spontaneous electrical activity of neurons in rat suprachiasmatic nucleus

The hypothalamic suprachiasmatic nucleus (SCN) is thought to be a light-entrained pacemaker in mammals, inducing a wide range of endogenous circadian events. In rat brain, histaminergic (HAergic) fibres are particulary rich in the hypothalamus. This prompted an investigation of the influence of bath-applied HAergic compounds on the spontaneous electrical activity of SCN neurons, recorded extracellularly in the hypothalamic slice preparation. Cells activated by bath application of HA ( n = 28) outnumbered those inhibited by HA ( n = 6). 48% of cells tested ( n = 28) were unresponsive to HA application. HA-induced activation of SCN neurons' discharge rate could be suppressed by the H 1-antagonist mepyramine, but not by the H 2-antagonist cimetidine. HAergic effects were still present when synaptic transmission was blocked, indicating a postsynaptic site of action for HA within SCN. Due to the anatomical distribution of HA-responsive SCN neurons and the independence of HA-effects with respect to circadian time, HA seems not to endow a pivotal role within generation and maintenance of circadian rhythm. Although data were obtained from SCN deprived of neural input, results show clear evidence for a modulatory impact of HA on the spontaneous electrical activity of part of SCN neurons.

Dual effect of glycine on isolated rat suprachiasmatic neurons.

Pharmacological properties of strychnine-sensitive and -insensitive glycine receptors have been investigated in rat suprachiasmatic nucleus (SCN) neurons. Because the SCN neurons were too small for stable intracellular recordings by the glass-microelectrode technique, a conventional whole cell mode patch-clamp technique was employed on the acutely dissociated SCN neurons. Dissociated SCN neurons were morphologically heterogeneous and could be distinguished into several types. All cells responded to glycine in a concentration-dependent manner. The glycine-induced current was primarily Cl- sensitive and competitively blocked by strychnine. The SCN neurons also responded to excitatory amino acids: glutamate, quisqualate, kainate, and N-methyl-D-aspartate (NMDA). Responses to glutamate and aspartate, which are endogenous neurotransmitter candidates, were enhanced by adding glycine. Glycine especially augmented the maximum response to NMDA in a full concentration range. 6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX) did not suppress the strychnine-sensitive glycine response but did suppress the strychnine-insensitive NMDA response in a competitive manner for glycine. The results suggest that glycine influences neural activity in the SCN as a classical inhibitory neurotransmitter and an excitatory neuromodulator.

Internalization of ( 125I) alpha-bungarotoxin into rat suprachiasmatic nucleus neurons and dendrites

The distribution and rate of internalization of ( 125I) alpha-bungarotoxin (α-BuTX) was evaluated by quantitative electron microscopic autoradiography in rat suprachiasmatic nucleus. The ultrastructural distribution of silver grains was studied by both line source and 50% probability circle analyses. Line source analysis demonstrated that with increasing time following intracerebroventricular infusion, ligand is internalized from neuronal membranes. At all time points, real grain distribution was significantly different from randomly generated hypothetical grains. Quantification of silver grain localization by probability circle analysis indicated that membrane bound sources were mainly associated with axo-dendritic appositions, regardless of the length of time the tissue was exposed to radioligand. The compartment containing synaptic terminals was the most enriched when comparing real to hypothetical grains. By eight hours after intracerebroventricular infusion of specifically labelled α-BuTX binding sites were most likely to be within neurons and dendrites. These studies demonstrate that the majority of α-BuTX binding sites remain membrane bound with respect to time and may be associated with synaptic transmission; a significant proportion of silver grains are internalized within SCN neurons and dendrites.

Neural regulation of circadian rhythms.

Neural regulation of circadian rhythms.