Arcuate Nucleus Of Rat Hypothalamus Research Articles

Electron-microscopic cytochemistry of the catecholaminergic innervation of ACTH-containing neurons in the rat hypothalamic arcuate nucleus.

The synaptic relationship between catecholamine terminals and adrenocorticotropic hormone (ACTH)-containing neurons in the arcuate nucleus (AN) of the rat hypothalamus was investigated by electron microscopy, using ACTH immunocytochemistry combined with autoradiography after 3H-dopamine (3H-DA) injection or 5-hydroxydopamine (5-OHDA) uptake in the same tissue section. ACTH-like (ACTH-LI) immunoreactive nerve cell bodies and fibers received synaptic inputs by axon terminals labeled with 3H-DA or 5-OHDA in the AN. This suggests that catecholaminergic neurons, at least DA- and 5-OHDA-containing neurons, may play an important role in the regulation of ACTH secretion or other functions of ACTH neurons via synapses in the AN of the rat hypothalamus.

Rapid effects of gonadal steroids upon hypothalamic neuronal membrane ultrastructure

Freeze-fracture methodology was used to study rat hypothalamic arcuate nucleus (AN) neuronal plasma membrane organization following in vitro perfusion of brain slices with 17-β-estradiol (17βE 2) or other test compounds. Physiological levels (10 −10 M) of 17βE 2 caused an increase in neuronal membrane exo-endocytotic pits within 1 min of perfusion. The increased density of pits was dose related, sustained at a constant rate during 10 min of perfusion, reverted to control values after perfusion with estradiol-free medium for 1 h, and was accompanied by an increased uptake of horseradish peroxidase by the arcuate nucleus in brain slices. The 17 βE 2-induced increase in exo-endocytotic pit density was blocked by tamoxifen (10 −8 M). Cholesterol (10 −10M), 17-α-estradiol (10 −6M) or dihydrotestosterone (10 −6 M) had no effect on exo-endocytotic pit density. Testosterone had about 50% the potency of 17 βE 2 in increasing exo-endocytotic pit density. These results indicate that physiological levels of 17βE 2 can have rapid effects upon arcuate nucleus neuronal membrane ultrastructure.

Neurotensin excites neurons in the arcuate nucleus of the rat hypothalamus in vitro

The effects of neurotensin (NT) on 41 spontaneously active neurons in the rat hypothalamic arcuate nucleus (ARC) were determined using an in vitro brain slice technique. Addition of NT (1 microM to 10 nM) to the perifusing medium allowed the action of known concentrations of NT to be examined. Concentrations of peptide greater than 10 nM evoked excessive depolarization of sensitive neurons resulting in long periods of postexcitatory inhibition. At 10 nM, NT evoked reversible and repeatable excitatory (20%) and inhibitory (25%) responses. When synaptic activity was blocked by lowering the Ca2+ and raising the Mg2+ concentration of the medium only excitatory responses (25% of units tested) were observed. These results demonstrate a potent excitatory action of NT within the hypothalamic ARC at the single cell level.

Neurons with whorl bodies have increased numbers of synapses

The endoplasmic reticulum in some neurons of the rat hypothalamic arcuate nucleus forms concentric sheets of smooth cisternae which are known as whorl bodies. It has been reported that the number and size of these structures change under varying endocrine conditions. For example, the number of rat arcuate nucleus neurons containing whorl bodies increases after gonadectomy. In studying their relationship to other components of the cell, we found that neuron profiles which contain whorl bodies receive a significantly increased number of axosomatic presynaptic terminals ( P < 0.001). Whorl bodies may mark a subpopulation of endocrine sensitive cells which are characterized by a different pattern of connections and whose response to stimuli includes changes in endoplasmic reticulum organization.

Coexistence of neuropeptide Y and somatostatin in rat and human cortical and rat hypothalamic neurons

The distribution and coexistence of neuropeptide Y (NPY) and somatostatin (SOM) were evaluated in rat and human cerebral cortex and in the rat hypothalamic arcuate nucleus (n) using double immunofluorescent staining in which primary antisera were raised in different species. The results of the study indicate extensive coexistence of NPY and SOM in both rat and human cortex but only occasional coexistence in the rat arcuate n.

Morphological evidence for synaptic junctions between substance P-containing neurons in the arcuate nucleus of the rat

The presence of synaptic junctions between substance P-containing neurons (SP neurons) was shown in the hypothalamic arcuate nucleus of rats by pre-embedding electron microscopic immunohistochemistry. The immunoreactive perikarya were synapsed by immunoreactive fiber terminals as well as immunonegative fiber terminals. The functional implication of the synapses between SP neurons is discussed in relation to autoregulatory mechanisms of the neurons.

Demonstration of contacts between proopiomelanocortin neurons in the rat hypothalamus

The relationships between proopiocortin neurons have been studied in the rat hypothalamic arcuate nucleus by immunocytochemistry. At the light microscopic level, contacts between immunostained endings and cell bodies or dendrites were frequently observed. At the electron microscopic level, stained nerve terminals were seen making symmetrical synaptic contacts with labeled dendrites and cell bodies. These observations are the first indication that neurons producing the same peptides can have contacts between themselves. This provides morphological evidence for autoregulation of neuropeptide systems.

Electronmicroscopic study of somatostatin-containing neurons in rat arcuate nucleus with special reference to neuronal regulation

After an intraventricular administration of colchicine, the arcuate nucleus of rat hypothalamus was examined light and electron microscopically by pre-embedding immunohistochemistry for somatostatin. The arcuate nucleus exhibited numerous immunoreactive cell bodies and dense networks of immunoreactive fibers. The fibers appeared to surround immunonegative cell bodies. The immunoreactive cell bodies were multipolar in shape and projected immunoreactive processes to some extent. The immunoreactive cell bodies and fibers received synaptic contacts by immunonegative fiber terminals containing a large number of synaptic clear vesicles. Similarly, immunoreactive somatostatin fibers appeared to terminate upon other immunonegative cell bodies and fibers. The immunoreactive presynaptic terminals contain several labeled granules and numerous synaptic vesicles. In close proximity to these immunolabeled terminals, non-labeled presynaptic fibers. This suggests that in the arcuate nucleus neurons regulated by somatostatin neurons are also under the control of other types of neurons.

ACTH-immunoreactive buotons form synaptic contacts in the hypothalamic arcuate nucleus of rat: evidence for local opiocortin connections

ACTH-like immunoreactive structures were localized in rat hypothalamic arcuate nucleus using the unlabeled antibody, peroxidase-antiperoxidase method. At the ultrastructural level, immunoreactive presynaptic nerve terminals were observed forming symmetrical synaptic contacts with unlabeled dendrites and with ACTH-like immunoreactive perikarya. The results obtained are consistent with the hypothesis that ACTH in the brain acts as a synaptic regulator or transmitter, contributing not only to long projection pathways but also to a local circuit in the arcuate nucleus where ACTH cell bodies are localized.

Hemigonadectomy-Induced Unilateral Changes in the Protein-Synthesizing Activity of the Rat Hypothalamic Arcuate Nucleus

The effect of unilateral ovariectomy on the protein-synthesizing activity of the hypothalamic arcuate and dorsomedial nucleus on both sides was studied in vivo as well as in vitro. Four weeks after the removal of 1 ovary, there was a significant increase in labelled amino acid incorporated into the arcuate neurons contralateral to the removed ovary as compared to those incorporated into the nerve cells of the nucleus on the ipsilateral side. In the dorsomedial nucleus, there was no difference between the 2 sides. On the basis of the present findings, the existence of a neural pathway between the ovary and the hypothalamic arcuate nucleus is assumed.

Thyroidectomy stimulates incorporation of [ 3H]cytidine into RNA in the arcuate nucleus of rat hypothalamus

Thyroidectomy stimulates incorporation of [ 3H]cytidine into RNA in the arcuate nucleus of rat hypothalamus

Data on protein synthesis of deafferented hypothalamic arcuate neurons.

Protein synthesizing activity of the rat hypothalamic arcuate nucleus following partial or total deafferentation of the medial basal hypothalamus was studied by light and electron microscopic autoradiography when administering tritiated leucine into the lateral ventricle. There were significantly more grains over the arcuate nucleus 21 days after disconnection of this hypothalamic region than over the intact nucleus. Isolation of a temporal cortical region induced similar changes in the isolated area, although this effect was not so pronounced as in the arcuate region. Data suggest that the protein synthesizing activity of arcuate neurons increases significantly after interruption of neural connections of the medial basal hypothalamus. It is assumed that the effect is primarily due to transneuronal alteration and/or interruption of inhibitory afferents.