Rare Specimens Research Articles

The bivalved arthropod Caudicaella aff. bispinata from the Heatherdale Shale (Cambrian Stage 3), South Australia

Sunellids are early Cambrian bivalved arthropods first recognized in South China that are important for understanding the early evolution of the group. However, their taxonomic affinity and distribution beyond South China is poorly known. Furthermore, the relationship between members of the Sunellidae and the globally dispersed Isoxys remains unclear. Recently, rare specimens of the sunellid Caudicaella aff. bispinata have been recovered from the lower Cambrian (Series 2, Stage 3) Heatherdale Shale, eastern Stansbury Basin, South Australia, representing the first record of the group from East Gondwana. Caudicaella aff. bispinata from Australia bears a semicircular carapace, a pair of anterodorsal sulci, anterior and posterior cardinal spines, as well as a distinct thick border or margin. It most closely resembles Caudicaella bispinata from the Shuijingtuo Formation in western Hubei Province, South China. The new discovery adds support for strong faunal similarity of the Konservat-Lagerstätten of South Australia and South China and extends the known geographical range of Cambrian sunellids to East Gondwana. The occurrence of Caudicaella bispinata in South China and Caudicaella aff. bispinata in East Gondwana strengthens the biostratigraphic correlation between those two palaeo-continents.

The Origin of the Chionoecetes Opilio Snow Crab Larvae in the Kara Sea

Most likely, the non-indigenous snow crab opilio, Chionoecetes opilio, entered the Kara Sea from the Barents Sea, both due to the migration of adults and with currents at the larval stage. At the moment, all bottom stages, including mature individuals and a large number of pelagic larvae are present in the Kara Sea. However, the origin of the larvae has not yet been clarified. The larvae that hatched in the Kara Sea should be at an earlier stage of development compared to the Barents Sea larvae that got here due, to later development of phytoplankton and, accordingly, later hatching. The larvae of the snow crab Chionoecetes opilio and the spider crab H. araneus were collected in the central and southwestern parts of the Kara Sea in July — early August 2019 by the Bongo zooplankton net 60 cm in diameter. It was established that the larvae were unevenly distributed across the Kara Sea. The main concentrations were found on the border with the Barents Sea in the St. Anna Trough (up to 860 ind./m2), and relatively high concentrations of larvae were noted in the southwestern part, where their abundance at the stations varied from 18 to 302 ind./m2. In the zone of the Ob-Yenisei plume, crab larvae were absent or their abundance was minimal. Using molecular genetic methods, the species identity of 361 larvae (344 C. opilio and 17 H. araneus) was reliably determined, and measurements of a number of morphological structures were made for 401 larvae. Significant differences in size at the zoea stage II between C. opilio and H. araneus were established. In most of the Kara Sea in July 2019, in zooplankton samples, crab larvae were represented by zoea I C. opilio with rare specimens of zoea I H. araneus of the Kara Sea origin. Only in the southwestern part, on the border with the Barents Sea, the presence of zoea II C. opilio and H. araneus was observed in samples with an increase in the proportion of the latter species in catches, which probably originate from the Barents Sea.

High-resolution three-dimensional atlas of congenital heart defects based on micro-CT images of human postmortem wax-infiltrated heart specimens

IntroductionPostmortem heart specimens are essential for education and research on the anatomy, morphology, and pathology of congenital heart defects. However, such specimens are rarely obtained these days, and the specimens stored in formalin are inexorably deteriorating. This study aimed to develop methods to archive three-dimensional data of rare human heart specimens and to publish the data. MethodsAll wax-infiltrated human postmortem heart specimens stored in the Cardiac Registry, Boston Children's Hospital were scanned using microfocus computed tomography (X-Tek HMXST225, Nikon Metrology, Inc.), and reproduced using a three-dimensional printer (Form 3B, Formlabs Inc.). The digital models were published as an interactive three-dimensional online atlas. The resolution of the three-dimensional data was evaluated. ResultsThe primary diagnoses in the 88 specimens included in the study include normal cardiac anatomy (11 cases), transposition of the great arteries {S,D,D} (11 cases), ventricular septal defect (10 cases), double-outlet right ventricle (9 cases), hypoplastic left heart syndrome (9 cases), and common atrioventricular canal (7 cases). Twenty-five cases (28%) underwent previous surgical or percutaneous interventions to the heart, including Mustard procedure (1 case), Senning procedure (2 cases, one was performed on a postmortem heart specimen). The median voxel size of the three-dimensional data was 40.5 um (IQR, 32.8–64.2). All intracardiac structures were precisely reproduced as digital and physical three-dimensional models. ConclusionsThe methods and resultant models were considered useful for archiving and furthering the utilization of these invaluable specimens. The atlas is available at https://www.sketchfab.com/heartmodels/collections.

Cultivated plants and their wild relatives of Central Russia and the North Caucasus (results of a collecting mission of 2023)

In 2023, field explorations of cultivated plants and their wild relatives were carried out in the regions of the Volga Upland, the western part of the Caspian Lowland and the Stavropol Upland with the aim of further replenishing seed and herbarium collections of VIR.A total of 525 samples of CWR and cultivated plants were collected, including 200 are herbarium sheets, 323 seed samples of cultivated plants and CWR, and a couple of cuttings. The collected material encompasses 145 species, including 91 cultivars. Seeds of the cultivars were purchased at markets, in supermarket chains and from local residents. The following areas were examined in detail: the left bank of the Lower Volga (Peschany anabranch, Kigach anabranch, as well as the “Novaya Roshcha” protected area). In the Republic of Ingushetia the team collected samples of cherry plum, Caucasian pear, barberry, viburnum, Lonicera orientalis, sea buckthorn, raspberry, and Malus orientalis, which local residents use in their diet. Also, samples of spicy herbs such as savory, catnip, oregano, thyme, etc. were collected. Hops were sampled in the Tambov and Volgograd provinces, the Republics of Ingushetia, North Ossetia and Adygea, from abandoned household plots in Botanika village in Gulkevichi District (Krasnodar Territory). The exploration in vicinities of Derbent and of the wall of Naryn-Kala fortress in Derbent resulted in collecting a rare specimen of Secale sylvestre Host., which previously used to be a fairly common plant in the North Caucasus, but is very rare now. Vegetable crops were collected along the entire route of the collecting mission. It was not always possible to collect seeds, so a part of the material enriched the herbarium collection. The collected material reflects the use of a vast range of vegetable and spicy plant species by the local population.Collecting missions help to understand which species are of primary importance for the conservation of natural processes, biodiversity and the maintenance of local ecosystems. The genetic diversity of a region is a key object of research for geneticists and plant breeders. Landraces of vegetable, cereal and other crops may have unique properties, such as adaptation to local climate conditions or disease resistance. Work on the mobilization and conservation of the plant genetic diversity is the basis for modern breeding and genetic research.

Two New Species of Elaphoidella (Copepoda, Harpacticoida) from Subterranean Waters in Northeast Thailand, with a Record of a Gynandromorphic Specimen and an Up-to-Date Key to Elaphoidella Species from Southeast Asia

Two new species of copepods of the genus Elaphoidella Chappuis, 1929 were discovered in a cave and a spring in northeastern Thailand. The first species, E. phuphamanensis sp. nov., belongs to species-group VII sensu Lang. It is most similar to E. turgisetosa Petkovski, 1980 in the armament of the male third exopod of the fourth swimming leg and the shape and armament of the fifth swimming leg in both sexes. However, it is easily distinguished from other congeners by the segmentation of the first swimming leg, the endopod of the fourth swimming leg, and the armature of the third exopod of swimming legs 2–4 in both sexes. The second species, E. propecabezasi sp. nov., is located in species-group I sensu Lang, where the male does not have a transformed seta on the third exopod of the fourth swimming leg and the female fifth swimming leg has four baseoendopodal robust setae, unequal in length. It is most similar to E. cabezasi Petkovski, 1982 and E. paraaffinis Watiroyram, Sanoamuang and Brancelj, 2017 in having the same armature formula as endopods 1–2 of female swimming legs 1–4. However, the ornamentation of the anal operculum, the shape of the caudal ramus, and the armature of the fifth swimming leg in both sexes distinguish them from each other. A rare gynandromorphic specimen of E. propecabezasi sp. nov. was recorded, and a revised key to Elaphoidella species in Southeast Asia is provided.

Learning Sciences from the Past: Recovery, Study, and Cataloging of a Historical Natural History School Museum

School–museum partnerships have gained considerable attention in the academic literature in recent decades. However, their role in enhancing science education is still an under-researched topic. This work reports on the outcomes of the recovery, study, cataloging, and valorization of the 18th century geo-mineralogical collection belonging to the Collegio Nazareno, now housed at the Istituto San Giuseppe Calasanzio in Rome. The project, which was participated in by the students enrolled in the fourth- and fifth-grade high school classes, revealed rare specimens such as the mineralogical collection donated by the Holy Roman Emperor Joseph II (1741–1740) in 1785. Furthermore, the laboratory activities organized from the stored samples highlighted educational approaches that helped students to cope with the complexities of authentic scientific work and cultural related issues. This work thus offers significant insights into the importance of natural history school museums as useful (and oft-forgotten) learning tools in science education.

Availability and issues of 3D-printed skull models for veterinary anatomy laboratories from students' perspective before and during the COVID-19 pandemic.

Three-dimensional (3D)-printed models of bones are a convenient and durable alternative to real bone specimens, and they have been used in anatomy laboratories. It is necessary to identify the precise advantages of 3D-printed models from all perspectives; not only the improvement in students' knowledge of anatomy but also the students' assessment of such models. Here, students of veterinary medicine and animal science evaluated the reproducibility and effectiveness of 3D-printed models as a learning tool by completing our questionnaires, with a focus on their understanding of the skull-morphological differences among dog breeds. With the COVID-19 pandemic having obliged veterinary universities to provide courses online, we also investigated how the pandemic affected the students' evaluation of the 3D-printed models. The questionnaire results revealed that the animal science students were satisfied with the reproducibility of the 3D-printed models, but the veterinary students were not (they preferred to use real specimens). The skull differences were well understood by both types of students, indicating that 3D-printed models are effective for learning about rare skeletal specimens. The veterinary students who experienced the COVID-19 pandemic tended to choose real specimens more often than those who did not have this experience. Our results suggest that the use of 3D-printed models as an introduction and the use of real specimens in anatomy laboratory courses can be adequate for veterinary students. Together our findings suggest ways to improve the educational performance of 3D-printed models for veterinary students who need to understand the anatomy of many species.

Evaluating methods for determining mercury concentrations in ancient marine fish and mammal bones as an approach to assessing millennial-scale fluctuations in marine ecosystems

Millennial-scale datasets of heavy metals in biota are difficult to obtain but are important for determining patterns and underlying drivers of toxicant concentrations. This is particularly important to better discriminate contemporary natural and anthropogenic sources. Globally mercury is a contaminant of concern. Post-industrial increases in mercury in arctic biota have been documented and monitoring of Steller sea lions (Eumetopias jubatus) in the Aleutian Islands, Alaska, has revealed a high proportion of pups with fur mercury concentrations above thresholds of concern in some regions. As bone is a tissue that is well preserved in archeological middens, it may prove useful for developing long-term mercury data sets under appropriate conditions. The goal of this study was to evaluate methodologies for measuring mercury concentration in Steller sea lion bone using a direct mercury analyzer, considering sample preparation methods and variability among bone tissue types (e.g., compact versus spongy bone). Finally, we directly compare sensitivity and precision of two different direct mercury analyzer models. Based on the methods presented here, direct mercury analysis using the Nippon MA-3000 can quantify small (ppb) quantities of Hg accurately and precisely in 20 to 60mg of bone with minimal specimen processing. The described method is efficient, relatively inexpensive, and requires minimal bone, conserving rare and valuable specimens. Hydrogen peroxide cleaning and collagen extraction were not required, and may be detrimental for optimal Hg quantification in bone. Further, while homogenization of distinct compact and spongy bone did not impact concentration determination, variance of technical replicates was lower improving quantitation precision. Most importantly, significant differences between compact and spongy bone exist within some individual specimen; however, the difference is not consistent and may indicate differential Hg exposure windows influenced by turnover rate of bone types. We conclude bone provides a natural archive for mercury ecosystem dynamics over millennial time scales in regions where appropriate samples are available. Compact bone has lower and less variable [THg] simplifying analysis and interpretation of data; however, the more dynamic concentrations observed in spongy bone should not be dismissed as invaluable due to their variability in [THg]. Comparisons of [THg] between bone type within individual may provide insight into more acute changes in mercury exposure within an individual’s lifetime.

‘Dawn’ hexapods in Cenozoic ambers (Diplura: Campodeoidea)

Abstract Diplura are an ancient group of basal (apterygote) hexapods that thrive in various cryptic terrestrial habitats. Despite an ancient origin that extends at least to the Devonian period, the dipluran fossil record is exceedingly sparse. Here, we document five very rare fossil specimens of the family Campodeidae in amber from the Miocene of the Dominican Republic and the Eocene of the Baltic region. Microscopic preservation in amber provides unique detail for taxonomic placement of small, delicate, soil- and leaf litter-dwelling organisms like these. New taxa include the following: in Lepidocampinae, Lepidocampa glaesi sp. nov. (in Dominican amber); and in Campodeinae, Litocampa eobaltica sp. nov. (in Baltic amber) and Rostricampa engeli gen. et sp. nov. (in Dominican amber). Rostricampa has an extraordinary rostrum formed by sclerotized extensions of the clypeus and, probably, the labium, unique among diplurans. These new taxa provide rare additional data on the fossil record of the earliest diverging lineages of the hexapods and shed light on their evolution and ecology.

Life in darkness: an overview of cave-adapted japygids (Hexapoda, Diplura)

Few species of Japygidae (Diplura) have been discovered in cave ecosystems despite their importance as large predators. A small collection of rare specimens of this hexapod group has allowed to explore the taxonomy of japygids from caves in New Zealand, Morocco and South Africa, and to describe one new genus: Imazighenjapyx Sendra & Sánchez-García gen. nov., as well as four new species: Austrjapyx wynbergensis Sendra & Sánchez-García sp. nov., Imazighenjapyx marocanus Sendra & Sánchez-García gen. et sp. nov., Opisthjapyx naledi Sendra & Sánchez-García sp. nov. and Teljapyx aotearoa Sendra & Sánchez-García sp. nov. For each of the new taxa we give a comprehensive description of their habitats. These new findings resulted in a revision of the distribution and allowed to re-evaluate the morphological traits of the fifteen cave-adapted japygids species already known worldwide. The functional morphology of the remarkable abdominal pincers of Japygidae and their adaptation to predation are discussed, as well as their potential role in mating behaviour.

Genetic Profiling and Genome-Scale Dropout Screening to Identify Therapeutic Targets in Mouse Models of Malignant Peripheral Nerve Sheath Tumor.

Malignant Peripheral Nerve Sheath Tumors (MPNSTs) are derived from Schwann cells or their precursors. In patients with the tumor susceptibility syndrome neurofibromatosis type 1 (NF1), MPNSTs are the most common malignancy and the leading cause of death. These rare and aggressive soft-tissue sarcomas offer a stark future, with 5-year disease-free survival rates of 34-60%. Treatment options for individuals with MPNSTs are disappointingly limited, with disfiguring surgery being the foremost treatment option. Many once-promising therapies such as tipifarnib, an inhibitor of Ras signaling, have failed clinically. Likewise, phase II clinical trials with erlotinib, which targets the epidermal growth factor (EFGR), and sorafenib, which targets the vascular endothelial growth factor receptor (VEGF), platelet-derived growth factor receptor (PDGF), and Raf, in combination with standard chemotherapy, have also failed to produce a response in patients. In recent years, functional genomic screening methods combined with genetic profiling of cancer cell lines have proven useful for identifying essential cytoplasmic signaling pathways and the development of target-specific therapies. In the case of rare tumor types, a variation of this approach known as cross-species comparative oncogenomics is increasingly being used to identify novel therapeutic targets. In cross-species comparative oncogenomics, genetic profiling and functional genomics are performed in genetically engineered mouse (GEM) models and the results are then validated in the rare human specimens and cell lines that are available. This paper describes how to identify candidate driver gene mutations in human and mouse MPNST cells using whole exome sequencing (WES). We then describe how to perform genome-scale shRNA screens to identify and compare critical signaling pathways in mouse and human MPNST cells and identify druggable targets in these pathways. These methodologies provide an effective approach to identifying new therapeutic targets in a variety of human cancer types.

Micro-CT imaging in species description: exploring beyond sclerotized structures in lichen moths (Lepidoptera: Erebidae, Arctiinae, Lithosiini).

X-ray micro-computed tomography imaging (micro-CT) is valuable for systematic research since it permits the non-destructive scanning and imaging of internal structures of very rare species and/or type specimens. Additionally, micro-CT allows to view the morphology and the functional anatomy of structures in their natural anatomical position, without deformations that typically occur using classical dissection protocols. In this study we provide the description of two new species of lichen moths (Lepidoptera: Erebidae, Lithosiini) from the Atlantic Forest in eastern Brazil: Nodozana heliae Moraes sp. nov. from Rio de Janeiro state and Epeiromulona pataxo Moraes & Aguiar sp. nov. from Bahia state. The male and female genitalia as well as the wing morphology were examined by means of non-destructive micro-CT, subsequent 3D model reconstruction, 360 degree spinning animations, 2D images from different angles, and those were compared against classical genitalia dissections from the same specimens. We conclude that techniques complement each other, micro-CT being particularly useful to study wing venation, sclerotized internal structures and muscles, while classical dissection is useful to study membranous structures, particularly in the female genitalia, abdominal skin and specialised scales on the male 8th sternite.

A phosphatised fossil Lagerstätte from the middle Cambrian (Wuliuan Stage) of North Greenland (Laurentia)

The upper Henson Gletscher Formation (Cambrian, Miaolingian Series, Wuliuan Stage) of southern Lauge Koch Land and Løndal, south-western Peary Land, North Greenland (Laurentia) yields diverse assemblages of phosphatised Small Shelly Fossils after treatment with weak acetic acid. The occurrence merits description as a fossil Lagerstätte on account of the exceptional preservation of soft parts in some specimens, although the phosphatisation itself is generally rather coarse. Bradoriid and phosphatocopid arthropods are common and display substantial variation in their preservational history. Some specimens retain traces of internal morphology, although details are generally obscured by indifferent preservation. Rare specimens extend the range of stem-group pentastomid arthropods back by more than 10 million years. A unique hatching larva demonstrates the same early developmental stage of a stem-group priapulan worm to that described in some present-day priapulans. The preservation of an in place operculum has demonstrated that the widespread, supposed mollusc Protowenella is actually an unusual, strongly coiled hyolith. Abundant coprolites and cololites provide additional evidence of early phosphatisation. In terms of morphology, Cambrocoryne lagenamorpha gen. et sp. nov. superficially resembles wiwaxiid and some annelid sclerites, thelodont scales and the foraminiferan Lagena, but its true identity is obscure.

Stressing environmental conditions in the “petrified forest” from the Mata Sequence in the Triassic context of the Paraná Basin

This study deals with the Piscina Outcrop, a Rhaetic reddish conglomeratic sandstone containing silicified logs which corresponds to the Mata Sequence in the southernmost Brazilian Paraná Basin. We performed ground-penetrating radar (GPR) analysis and the results ratified previous sequence stratigraphy analysis indicating that these deposits correspond to architectural elements exclusively related with the filling processes of fluvial channels. The erosive unconformity identified at the boundary between the Mata Sequence and the underlying rock packages could represent a considerable depositional hiatus. The integration of the GPR results with sedimentological and paleovertebrate data from a nearby outcrop at slightly lower topographic level indicated that the layer with the fossil woods is superimposed through an erosive unconformity to the stratigraphic levels corresponding to the Hyperodapedon Assemblage Zone of Carnian age from the basal portion of the Candelária Sequence. The wood assemblage was composed exclusively of araucarian coniferous stems identified as Agathoxylon (cf. Agathoxylon). The wood growth patterns were mainly characterized by woods showing indistinct growth rings, with latewood mostly restricted to one cell in each ring, suggesting weak seasonality and short-term, periodic droughts. Rare specimens with absent growth rings were also present. Oscillations in growth ring width and the common occurrence of false rings were likely linked to plant exposure to climatic instability. Anatomical evidence pointed to active biochemical responses to intense and extensive biological attack of fungi and arthropods. The dominant presence of carbon in the elemental composition of a dark substance filling many tracheid lumens suggested a very likely affinity with polyphenol remnants in biochemical barriers. The set of wood signatures seems to be linked to regional stressing conditions rather than to the paleolatitudinal location when compared to other key South American basins during the Rhaetic. An allochthonous taphocoenosis is the most likely taphonomic interpretation for this wood assemblage. The logs were probably incorporated into the stream through the collapse of riparian areas and buried in the fluvial channel by high-energy flows after biotic and abiotic decay processes during lifetime and secondarily through exposition before entombment in a river channel.

A unique Silandos medallion of Faustina II from Blaundos in Lydia

AbstractDuring the 2019 archaeological excavation season in Blaundos, a Roman-period bronze medallion was found within a wall of a Byzantine-period structure on the main street. It is a medallion struck by the Lydian city of Silandos, bearing the bust of Faustina II on the obverse and Marcus Aurelius clasping hands with Lucius Verus on the reverse. A literature search revealed that it is a rare and unpublished specimen. NeitherRPC, the largest database of Roman Provincial Coinage, nor auction databases record any example of it. The iconography, combining the portrait of the empress with a depiction of the co-emperors of the period, does not point to any specific event or incident. The reverse die was, however, used for another medallion struck later by Silandos. This paper aims to introduce, interpret, and discuss this unique Lydian medallion from the 2nd c. CE.

Simple Universal Whole-Organ Resin-Embedding Protocol for Display of Anatomical Structures.

Whole-organ plastic resin casting is a very useful method for preserving rare pathological specimens for forensic/anatomical studies and for teaching/research purposes. Many techniques have been proposed over time, but most of them use special non-commercially available resin mixtures, lengthy protocols, and are overall not easily implemented in any anatomy/pathology department that might need such a procedure for rapid organ preservation. Here, we utilized anatomical sections of the human brain, heart, kidneys, spleen, large intestine, and lungs from on-display organs that were fixed for more than 1 year in 10% neutral-buffered formalin and from a freshly processed cadaver for teaching purposes in our Human Anatomy Department, and we optimized a fast-processing protocol without the use of any clearing agents, which yields solid, clear, cylindrical resin casting blocks. The resulting protocol, which takes no longer than 4 days, proves that at least three commonly used epoxy resins from hobby shops can be utilized without any restrictions, and the use of resin or glycerin vacuum-forced impregnation even offers two choices of intrinsic contrast, depending on the nature of the preparation. A number of innovations have been included here and compared to existing publications, such as the use of a system of permanent fixation plexiglas rods that maintain the organ in the desired position and become invisible in the final block, the use of UVC sterilization of the tissue to ensure a long shelf life of the block, and the utilization of cheap cylindrical polypropylene food containers as casting molds. Altogether, we present a simple resin-embedding protocol that can be made available to any department/institution without the need for expensive materials and specially trained personnel.

Ancient DNA Contradicts the Presence of Social Voles (Genus Microtus, Subgenus Sumeriomys) in the Late Pleistocene of Western Europe

Taxonomic decisions made by palaeontologists are often based on a few morphological features preserved in the fossil material. This practice may sometimes lead to the description of new species based on single specimens, which are, in fact, extreme or aberrant morphological variants of known taxa. Ancient DNA (aDNA) analysis of the Late Pleistocene specimens from the archaeological site Petits Guinards (Creuzier-la-Vieux, Allier, France), described as a new vole Microtus (Sumeriomys) bifrons, did not confirm the species distinctness of the studied population. The genetically examined specimens belonged to Stenocranius anglicus and/or Microtus arvalis, the dominant species at the site. Our findings show that it is risky to describe new fossil taxa on the basis of phenotypic outliers or morphologically aberrant, rare specimens that do not fall within the previously known population variability. We also highlight the importance of ancient DNA in resolving taxonomic and nomenclature problems and classifying fossil mammals of the Late Pleistocene age.

Abstract 5637: Path to the holy grail of spatial biology: Spatial single-cell whole transcriptomes using 6000-plex spatial molecular imaging on FFPE tissue

Abstract Cancer research across drug development, molecular biomarkers, and patient response depends on understanding biology that is dependent on complex interactions between malignant, immune, and stromal cells. To survive clearance mechanisms, a tumor can rely on a myriad of escape strategies, and the microenvironment is architected around the current path of escape. To enable a more comprehensive picture of tumor biology, we have developed the CosMx™ Spatial Molecular Imager (SMI) technology to capture a snapshot of thousands of RNA species resolved subcellularly from a single, standard histopathology slide. Building upon the previously released panels, this study tests a new 6,000-plex panel, the highest RNA plex measured in situ within human tissue, allowing the imputation of a spatial whole transcriptome in the tissue. We performed an ultra-high-plex RNA assay to detect 6,000 targets simultaneously in situ on an FFPE human liver cancer tissue (~1 cm2 area) using the CosMx SMI. This RNA panel covers broad biological areas with special emphasis on oncology, immunology, and signal transduction, such that all cancer researchers can benefit from the direct detection of targets of interest (sans imputation) in intact tissue. Analysis algorithms were developed to allow robust assessments of cell types, cell states, cell-cell interactions, and pathway activation. Imputation based on reference profiles from HCA, TCGA, and other public repositories allows estimation of non-measured transcripts at a ratio of approximately 1:3, compared to the approximate 1:20-1:70 imputations performed previously for spatial data.Thousands of transcripts were simultaneously detected with high sensitivity and specificity on the FFPE liver cancer tissue section at single-cell subcellular resolution. We were able to accurately map known reference profiles from scRNA-seq into this sample while identifying cancer-specific malignant, immune, and stromal cells in this tissue sample using this ultra-high plex RNA panel. In addition, we constructed sample-specific spatial neighborhoods, defined by cell types, cell states, and nearly unlimited sets of biological pathways through the imputed whole transcriptome. Finally, we measured >1,000 ligand-receptor interactions between key cell types of adjacent cells in the tissue, identifying mechanisms for tumor-mediated escape as well as reactive re-architecting of the native stroma which defines the trajectory of cancer’s evolution. Single-cell spatial measurements of gene expression at 6,000 plex from a single FFPE slide has the potential to transform our understanding of tumor biology and facilitate the next advances in cancer research by extracting the highest data density possible from rare specimens collected during patient treatment. Citation Format: Shanshan He, Michael Patrick, Jason W. Reeves, Patrick Danaher, Julian Preciado, Joseph Phan, Erin Piazza, Zachary Reitz, Lidan Wu, Rustem Khafizov, Haiyan Zhai, Michael Rhodes, David Ruff, Joseph Beechem. Path to the holy grail of spatial biology: Spatial single-cell whole transcriptomes using 6000-plex spatial molecular imaging on FFPE tissue. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 5637.

Rhinodrilus priscilae and Andiorrhinus (Amazonidrilus) caxiuana (Crassiclitellata, Rcrilidae) two new species from Caxiuanã National Forest

The amazon forest is the habitat of a high species diversity of invertebrates and unfortunately the real richness had been underestimated. Earthworms' inventories had been poor because difficult to describe new species. This study adds new records to eastern amazon of two new species belong Rhinodrilus and Andiorrhinus genera. R. priscilae n. sp. is another rare specimen of the genre having two pair of prostatoid glands. A. (Amazonidrilus) caxiuana differ of other species in size, the intestinal origin is in XXVIII and also has only one glandular mass associated to spermatheca.

Fossil diversity in ‘dawn’ hexapods (Diplura: Projapygoidea), with direct evidence for being chemically predaceous in the Cretaceous

Abstract Diplurans are among the earliest hexapods in the geological record. These primitively wingless relatives of insects are infrequently encountered despite being pervasive in soil habitats. Two groups have disparate adaptations for hunting, one mechanical and the other chemical, in Japygoidea and Projapygoidea, respectively. Here, we report three genera (two new) and four species of fossil Projapygidae preserved in Mesozoic and Cenozoic ambers: Electroprojapyx alchemicus gen. et sp. nov. in mid-Cretaceous Myanmar amber, and Symphylurinopsis punctatus gen. et sp. nov. and two species (unnamed) of Symphylurinus in Miocene Dominican amber. The exceptionally preserved specimens possess cerci that are morphologically specialized for expelling a glandular substance on prey. The new Cretaceous E. alchemicus is a stem group to all living species of the family and provides conclusive Mesozoic evidence for a rare type of predation involving offensive (vs. defensive) secretions. The specimen was fossilized in a preying-and-spraying position with its presumptive meal, a Symphypleona springtail (Collembola), reflecting the behavioural predatory repertoire of modern relatives. Apart from one Cenozoic species, these rare specimens represent the only fossil record of the family Projapygidae. Our findings demonstrate that these basal hexapods were predators of small arthropods of ancient forest soils and enrich our understanding of palaeoecological associations and behavioural strategies paramount for the survival of species.