Rapid Screening Of Antioxidants Research Articles

Rapid screening of antioxidant from natural products by AAPH-Incubating HPLC-DAD-HR MS/MS method: A case study of Gardenia jasminoides fruit

A new AAPH-Incubating HPLC-DAD-HR MS/MS method was developed for the rapid and high-throughput screening of antioxidants directly in natural products and applied to Gardenia jasminoides fruit. This method was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be significantly reduced or disappeared after incubating with the AAPH which can release ROO at physiological conditions (37 °C, pH 7.4). Additionally, the activity of antioxidants can be evaluated by comparing the peak reduction rates and the screened components can be further identified by HRMS/MS. Then, 17 potential natural antioxidants from the crude extract of GJF was screened. Among them, three major components including crocin I, crocin II and crocetin showed excellent ROO scavenging activity, which were further validated by the ORAC assay. In conclusion, our study provided a simple and effective strategy to rapidly screen antioxidants in natural products.

A method of lipid peroxidation in Paramecium tetraurelia

Lipid peroxidation in vitro has been examined in the ciliated protozoan Paramecium tetraurelia. A method is described which measures the susceptibility of Paramecium tetraurelia to lipid peroxidation induced by ascorbic acid and ferrous ion, as determined by production of thlobarblturic acid-reactive substances (TBARS). The paramecium model has been optimized with regard to pH, incubation time, incubation temperature, culture density, and other parameters. The method was observed to be sensitive and reliable, and yielded results comparable to various mammalian peroxidation models. The value of this technique lies in the growing use of Paramecium tetraurelia as models of diseases or conditions in which oxidative damage has been strongly implicated, including radiation toxicity, environmental toxicology, and aging, in addition, this method allows for the rapid screening of antioxidants both in vivo and in vitro.