Abstract
Lipid peroxidation in vitro has been examined in the ciliated protozoan Paramecium tetraurelia. A method is described which measures the susceptibility of Paramecium tetraurelia to lipid peroxidation induced by ascorbic acid and ferrous ion, as determined by production of thlobarblturic acid-reactive substances (TBARS). The paramecium model has been optimized with regard to pH, incubation time, incubation temperature, culture density, and other parameters. The method was observed to be sensitive and reliable, and yielded results comparable to various mammalian peroxidation models. The value of this technique lies in the growing use of Paramecium tetraurelia as models of diseases or conditions in which oxidative damage has been strongly implicated, including radiation toxicity, environmental toxicology, and aging, in addition, this method allows for the rapid screening of antioxidants both in vivo and in vitro.
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