Rapid Sample Preparation Method Research Articles

Concise Affinity-Based Purification of Ligated mRNA for Structure-Activity Relationship Studies ofNucleosugar Modification Patterns.

Position-specific nucleoside sugar modifications have been shown to improve the translational activity and stability of chemically synthesized mRNA. For pharmaceutical applications of chemically modified mRNA, a rapid purification methodology is imperative to identify the optimal modification pattern. However, while the chemical synthesis of mRNAs can be accomplished by splint ligation of oligonucleotide fragments, the current purification method for ligated mRNAs based on denaturing polyacrylamide gel electrophoresis tends to be time consuming. In this study, we developed a two-step affinity purification method for rapid sample preparation. In this method, ligated mRNA is captured by oligo dT magnetic beads and streptavidin magnetic beads with 3'-biotinylated oligo DNA, which are complementary to the 3'-poly(A) and 5' terminal sequences of the target mRNA, respectively. Therefore, the target mRNA can be isolated from a complex mixture of splint ligations. Using this method, six sugar-modified mRNAs were simultaneously purified, and the translational activities of these mRNAs were evaluated immediately after purification. The results demonstrate that this methodology is suitable for the rapid preparation of various chemically synthesized mRNAs to identify their optimal modification patterns.

An eco-friendly kapok fiber-supported liquid microextraction using natural deep eutectic solvent as extractant for convenient biological samples preparations: A proof-of-concept study

The use of natural materials and reagents is crucial for developing environmentally friendly methods. This study proposed an excellent green, convenient, cost-effective and rapid sample preparation method called kapok fiber-supported liquid-phase microextraction using natural deep eutectic solvent (KF-SLME-NADES). Natural kapok fiber and NADES were used as greener alternatives to conventional support medium and extractants. Low-toxic ethanol was employed as the desorption solvent. The extraction process was streamlined in a syringe form, allowing for simple pull-push operations. As a proof of concept, the developed methodology was utilized for extracting antidepressants from 3 mL of diluted biological fluids. The optimized KF-SLME-NADES conditions were obtained by single-factor experiments and Box-Behnken design as follows: 2 mg of kapok fiber, 13 μL of NADES composed of menthol and thymol in an equal molar ratio as the extractant, a sampling solution with a pH of 8.0, a desorption solution of 100 μL of ethanol, and four pull-push cycles for both extraction and elution. The validated method provided good linearity (0.05–5.00 μg/mL, R2 > 0.999) and possessed limits of quantification spanning from 0.025 to 0.048 μg/mL. Satisfied accuracies and precisions were obtained with relative recoveries ranging in 93.3–107.2% and intra- and inter-day RSD(%) being below 7.7%. The method greenness was found to be excellent by assessing five greenness evaluation metrics. The devised KF-SLME-NADES strategy provides a sustainable and effective method for bioanalysis.

Application of a Green Deep Eutectic Solvent for Preconcentration and Determination of Copper and Cadmium in Food, Cosmetic, and Water Samples

Background:: Determination of environmental pollutants is important due to their harmful effects on the health of living organisms. However, direct measurement of many of these pollutants is not possible due to their low concentrations, which necessitates the need for sample preparation methods. One of the rapid and simple sample preparation methods is the Air-Agitated Liquid-Liquid Microextraction (AALLME) method, which requires toxic organic solvents that can lead to environmental pollution. Therefore, finding green solvents like Deep Eutectic Solvents (DESs) to replace them can be investigated. Methods:: A novel Deep Eutectic Solvent (DES) was synthesized from a combination of choline chloride and 2-phenylethanol with a molar ratio of 1:4, and it was examined using FT-IR analysis. This solvent was used in an AALLME method for measuring copper and cadmium heavy metal ions in complex real samples with Flame Atomic Absorption Spectroscopy (FAAS). Results:: The influential parameters of this method, such as solution pH (5.4), extraction cycles (12), and extraction solvent volume (440 μL), were optimized utilizing central composite design (CCD). Underneath the optimized circumstances, the detection limits for Cu2+ and Cd2+ were 0.14 and 0.09 ng mL-1, and the linear dynamic range was 0.47-50.0 and 0.32-22.5 ng mL-1, respectively. The preconcentration factors for these cations were 139.7 and 133.4, respectively. Conclusion:: Combining this novel green solvent and rapid sample preparation method for the preconcentration and determination of the studied heavy metal ions has shown promising results in terms of enrichment factors and detection limit values.

Determination of pesticide residues and heavy metals in Adhatoda Vasaka Linn.

Heavy metals and pesticide residue analysis plays an important role in the quality control of medicinal plants like Vasaka (Adhatoda vasica). Hence a study was conducted to determine heavy metals and pesticide residues in this medicinal herb, which is a highly useful commodity in the health system. The reliable, rapid and nontoxic sample preparation method like QuEChERS and analytical methods like GC-MS were proposed for the simultaneous determination of pesticide residues and Heavy metal detection was carried out by ICP-MS. In this study, the presence of organophosphorus and organochlorine pesticides like alachlor, heptachlor, heptachlor epoxide, aldrin, dieldrin, endrin, etc was checked but not detected. Heavy metals like Arsenic (As), Cadmium (Cd), Mercury (Hg) and Lead (Pb) are traced in samples about 2.3005 ppb, 0.799 ppb, 2.290 ppb and 10.204 ppb respectively in the present study.

Study of Bitespiramycin Distribution in Rats and Cerebrospinal Fluid of Patients by a Sensitive LC-MS/MS Method with Rapid Sample Preparation.

Bitespiramycin, has been shown to have a therapeutic effect against respiratory tract inflammation, including a potential effect against COVID-19. A current clinical trial in China showed that bitespiramycin was an effective treatment for severe pneumonia and intracranial infection. However, there is lack of an analytical method to elucidate the distribution of bitespiramycin. In this study, a highly sensitive, rapid and reliable UPLC-MS/MS method was developed to comprehensively characterize the bitespiramycin distribution in various bio-samples, which is significantly improved upon the published work. A rapid sample preparation method was developed by using n-butanol as the solvent to extract bitespiramycin from different bio-samples. The extract was then directly analyzed by UPLC-MS/MS coupled with an alkaline-resistant column after centrifugation which avoids the time-consuming concentration process under nitrogen and redissolution. The method was employed to accurately quantify bitespiramycin and its metabolites in rat plasma, tissues, and human cerebrospinal fluid. Notably, the presence of bitespiramycin and its metabolites was identified for the first time in various rat organs including brain, testis, bladder and prostate as well as in human cerebrospinal fluid. This newly developed approach shows great promise for drug distribution assays including other antibiotics and can help elucidate the ADME of bitespiramycin.

Development and validation of a microwave-assisted digestion technique as a rapid sample preparation method for the estimation of selenium in pharmaceutical dosage forms by ICP-OES.

Selenium is a class 2B element according to the International Council for Harmonization Q3D guidelines. Selenium sulfide is an anti-infective agent with antifungal and antibacterial properties used to treat dandruff and seborrheic dermatitis. The literature survey revealed that most of the analytical techniques to estimate selenium were time-consuming and/or required high skill levels. The process involved identifying the isotopes, selecting the measurement approach, and optimizing a typical microwave-aided digesting procedure. Ammonium hydrogen difluoride, water, and concentrated nitric acid were added to the samples. The confirmed microwave digestion program was a two-step program where in the initial step, the samples were ramped at 200°C for 20 min and held for 5 min. Later, samples were cooled and neutralized by boric acid, then ramped for 20 min to a temperature of 180°C and held for 10 min. Selenium was estimated at 196.090 nm by inductively coupled plasma optical emission spectroscopy (ICP-OES). System suitability was run before initiating analysis to ensure that system performance was consistent. Analytical validation parameters, such as the specificity of the method, were demonstrated at 196.090 nm, linearity was proven from 10 ppm to 150 ppm of selenium concentration, the detection limit was 1.28 ppm, and the limit of quantification was 3.89 ppm. Robustness was confirmed for small changes to ICP-OES operating conditions. The precision of the method demonstrated by analyzing the percentage relative standard deviation for six injections was found to be less than 2.0%. Accuracy was confirmed from 10 ppm to 150 ppm, and all the samples were observed to be within the range of 95%-105%. A common microwave-assisted digestion technique was developed and validated as well. The precision, specificity, linearity, accuracy, and robustness of the method for estimating selenium in selenium sulfide drug substances and various pharmaceutical dosage forms were demonstrated. This newly developed microwave-assisted digestion technique has optimum sensitivity and is highly reproducible and time-saving than the existing methods This method can be applied to numerous matrices for a finished dosage of selenium sulfide formulations.

Comparison of test strip, conductivity, and novel smartphone digital image colorimetry methods for field assessment of soil chloride and salinity.

Understanding the extent of salt-affected soils is important in agriculture for irrigation, crop selection and land management, and affordable smartphone-based techniques can broaden access to environmental analysis in resource-limited settings. A novel smartphone-based Digital Image Colorimetry (DImC) technique using colorimetric chloride test strips has been developed and compared to 3 other field techniques (direct soil conductivity (Csoil), soil extract solution conductivity (Cext), titration test strips (TS)) and 1 lab-based method (chloride analyser (CA)) for the assessment of soil salinity. A survey of soils from a coastal saltmarsh and neighbouring farmland was conducted in Southwest Scotland and a rapid sample preparation method suitable for in-field use was evaluated. Comparison of each method with the reference method (CA) using correlation plots, Bland-Altman plots, and concordance correlation coefficients, showed that the accuracy and precision of the methods decreased in the order Cext > DImC ≃ TS > Csoil. The novel DImC method had good accuracy, although the limit of detection (LOD = 20.30 mg L-1) and precision was poorer than Cext. The analysis of field-wet soils enabled a rapid estimate of soil Cl- in approximately 6 minutes and spatially interpolated maps of soil Cl- concentration, prepared using smartphone location data, identified areas of elevated soil salinity. Each field analysis method had specific benefits, and the low cost, portability and lack of specialist equipment required for the DImC method demonstrated suitability for use in agriculture, citizen science or conservation settings where resources may be limited. The example presented demonstrates how smartphones may be used to broaden access to environmental analysis techniques.

Optimized Suspension Trapping Method for Phosphoproteomics Sample Preparation.

A successful mass spectrometry-based phosphoproteomics analysis relies on effective sample preparation strategies. Suspension trapping (S-Trap) is a novel, rapid, and universal method of sample preparation that is increasingly applied in bottom-up proteomics studies. However, the performance of the S-Trap protocol for phosphoproteomics studies is unclear. In the existing S-Trap protocol, the addition of phosphoric acid (PA) and methanol buffer creates a fine protein suspension to capture proteins on a filter and is a critical step for subsequent protein digestion. Herein, we demonstrate that this addition of PA is detrimental to downstream phosphopeptide enrichment, rendering the standard S-Trap protocol suboptimal for phosphoproteomics. In this study, the performance of the S-Trap digestion for proteomics and phosphoproteomics is systematically evaluated in large-scale and small-scale samples. The results of this comparative analysis show that an optimized S-Trap approach, where trifluoroacetic acid is substituted for PA, is a simple and effective method to prepare samples for phosphoproteomics. Our optimized S-Trap protocol is applied to extracellular vesicles to demonstrate superior sample preparation workflow for low-abundance, membrane-rich samples.

Using mandelic acid as an extraction solvent in the extraction of Cu(II) and Cd(II) from soil samples.

In this study, an efficient, green, and rapid sample preparation method based on mandelic acid dimer was proposed for the extraction of Cu(II) and Cd(II) from soil samples followed by flame atomic absorption spectrometry. In this research, for the first time, the liquid dimer was prepared by heating solid mandelic acid. Then the mixture of soil and a complexing agent was added into it. The mixture was transferred into a microwave oven. Diluted nitric acid solution as a dilution solvent was added. After centrifugation, two aliquots of the collected phase were removed and injected into the instrument. The relevant optimization parameters such as dimer volume, microwave exposure time, amount of complexing agent, and the type and volume of dilution solvent were investigated and optimized. Under the optimum conditions, detection limits were obtained 0.17 and 0.16mgKg-1 for Cu(II) and Cd(II), respectively. The linear ranges were 0.50-50mgKg-1 with coefficient of determination ≥ 0.9981. The developed method along with a reference method was applied for the analysis of the selected heavy metal ions in different soil samples and comparable results were obtained. Also, the method was performed on a certified reference material and the obtained concentrations compared with the certificated concentrations to assess accuracy of the proposed method.

A rapid and convenient sample preparation method for the analysis of cannabinoids in oral fluid samples.

Cannabis is the world's most used illegal drug. The main psychoactive component of cannabis is Δ9-tetrahydrocannabinol (THC). To aid the identification of cannabis-impaired individuals, a simple but effective workflow for reliable quantification of THC and its metabolites in oral fluid samples collected with the Greiner Bio-One Saliva Collection System is presented. Sampling involves rinsing the oral cavity with an extraction solution containing a citrate buffer stimulating salivary flow. Sample processing targeted the cannabinoid fraction interacting with proteins and other insoluble constituents that can be separated by centrifugation. Approximately 50% of the total amount of cannabinoids included in the oral fluid was recovered from the obtained pellet by extraction with acetonitrile. Liquid chromatography-tandem mass spectrometry was used for cannabinoid quantification. Fitness of the developed workflow for application in forensic and clinical cannabis testing was demonstrated by evaluating multiple performance parameters, including selectivity, linearity, limits of quantification (LOQs), accuracy, precision, matrix effects, extraction recoveries, process efficiencies and stability. Furthermore, sensitivity and specificity of the developed oral fluid-based cannabis test was demonstrated by analysing 195 samples collected either from opioid addicts or persons suspected of driving under the influence of drugs. The accuracy of identifying a person with the presence of THC in blood was found to be 97.9%.

Sample Preparation Methods for Targeted Single-Cell Proteomics.

We compared three cell isolation and two proteomic sample preparation methods for single-cell and near-single-cell analysis. Whole blood was used to quantify hemoglobin (Hb) and glycated-Hb (gly-Hb) in erythrocytes using targeted mass spectrometry and stable isotope-labeled standard peptides. Each method differed in cell isolation and sample preparation as follows: 1) FACS and automated preparation in one-pot for trace samples (autoPOTS); 2) limited dilution via microscopy and a novel rapid one-pot sample preparation method that circumvented the need for the solid-phase extraction, low-volume liquid handling instrumentation and humidified incubation chamber; and 3) CellenONE-based cell isolation and the same one-pot sample preparation method used for limited dilution. Only the CellenONE device routinely isolated single-cells from which Hb was measured to be 540-660 amol per red blood cell (RBC), which was comparable to the calculated SI reference range for mean corpuscular hemoglobin (390-540 amol/RBC). FACSAria sorter and limited dilution could routinely isolate single-digit cell numbers, to reliably quantify CMV-Hb heterogeneity. Finally, we observed that repeated measures, using 5-25 RBCs obtained from N = 10 blood donors, could be used as an alternative and more efficient strategy than single RBC analysis to measure protein heterogeneity, which revealed multimodal distribution, unique for each individual.

Development of an In-Field Real-Time LAMP Assay for Rapid Detection of Tomato Leaf Curl New Delhi Virus

Tomato leaf curl New Delhi virus (ToLCNDV) represents a threat to economically important horticultural crops. A real-time loop-mediated isothermal amplification (LAMP) assay for in-field ToLCNDV detection was developed, coupled to a rapid sample preparation method, and tested both in field and laboratory conditions on zucchini squash, tomato, and pepper samples. A set of six LAMP primers was designed for specific ToCLNDV detection, targeting a 218-nucleotide sequence within the AV1 gene. The sensitivity, specificity and accuracy of the real-time LAMP assay and comparison with canonical PCR were evaluated. The real-time LAMP assay developed was about one-thousand times more sensitive than the conventional PCR method, detecting a total of 4.41 × 102 genome copies as minimum target; no cross-reactivity was detected with the other geminiviruses used as the outgroup. The rapid sample preparation method allows for a reliable detection with a low reaction delay (≈2-3 min) compared to canonical DNA extraction, providing results in less than 45 min. Lastly, an increase in ToLCNDV-positive sample detection was observed compared to PCR, in particular for asymptomatic plants (85% and 71.6%, respectively). The real-time LAMP assay developed is a rapid, simple, specific, and sensitive technique for ToLCNDV detection, and it can be adopted as a routine test, for both in-field and laboratory conditions.

High-throughput quantitation of serological dimethylarginines by LC/MS/MS: Potential cardiovascular biomarkers for rheumatoid arthritis

Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by systemic inflammation of the joints and extra-articular tissues. The incidence of cardiovascular disease (CVD) remains the main cause of morbidity and mortality in patients with RA. Despite the development of new therapeutics targeting the articular manifestations, the relief of the cardiovascular burden is still an unmet medical need during the management of RA. So, the early prognosis of RA-associated CVD plays a crucial role in improving the clinical outcomes of RA patients. Recently, circulating dimethylarginines have gained attention as potential biomarkers for CVDs. Here, we present the development and validation of a high-throughput liquid chromatography-tandem mass spectrometric (LC/MS/MS) method for simultaneous quantification of creatinine, arginine, and dimethylarginines in human serum within 2 mins by isotope dilution mass spectrometry. This method employed a protein precipitation method for rapid sample preparation, trichloroacetic acid (TCA)-based ion pairing chromatography for fast analyte separation, and multiple reaction monitoring (MRM) with stable isotope-labeled internal standards (ISs) for simultaneous quantitation. To assure the quality, our method was validated against the FDA guidelines for lower limit of quantitation (0.2 µM), linearity (square of coefficient correlation>0.99), precision (intra-&inter-assay imprecision < 10 %), accuracy (intra-&inter-assay inaccuracy < 10 %), sample preparation recovery (recovery ≥ 90 %), stability (instability < 10 %), matrix effect (signal suppression < 55 %), and carryover ( < 0.01 %). Afterward, we applied the validated method to a retrospective cross-sectional study. We aimed to evaluate the utility of serological dimethylarginines as potential cardiovascular biomarkers in the development of RA-associated CVD. Our results revealed that the serological ratio of asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA), an indicator of physiological arginine methylation status, was significantly elevated in patients with RA. This finding might provide value in detecting CVD to improve clinical outcomes in RA management.

Rapid culture-independent loop-mediated isothermal amplification detection of antimicrobial resistance markers from environmental water samples.

Environmental water is considered one of the main vehicles for the transmission of antimicrobial resistance (AMR), posing an increasing threat to humans and animals health. Continuous efforts are being made to eliminate AMR; however, the detection of AMR pathogens from water samples often requires at least one culture step, which is time-consuming and can limit sensitivity. In this study, we employed comparative genomics to identify the prevalence of AMR genes within among: Escherichia coli, Klebsiella, Salmonella enterica and Acinetobacter, using publicly available genomes. The mcr-1, blaKPC (KPC-1 to KPC-4 alleles), blaOXA-48, blaOXA-23 and blaVIM (VIM-1 and VIM-2 alleles) genes are of great medical and veterinary significance, thus were selected as targets for the development of isothermal loop-mediated amplification (LAMP) detection assays. We also developed a rapid and sensitive sample preparation method for an integrated culture-independent LAMP-based detection from water samples. The developed assays successfully detected the five AMR gene markers from pond water within 1h and were 100% sensitive and specific with a detection limit of 0.0625 μg/mL and 10cfu/mL for genomic DNA and spiked bacterial cells, respectively. The integrated detection can be easily implemented in resource-limited areas to enhance One Health AMR surveillances and improve diagnostics.

An alternative method of dispersive solid‐phase extracting Hg (II) from environmental aqueous solutions using carboxylic functionalized carbon nanotubes

This study is a simple method for rapid sample preparation before instrumental experiment, which is an important tool and the main step in an analytical proceeding. Till now, different sample preparation procedures based on solid-phase extraction manner were developed to isolate different types of analytes from different matrices. In this method, the preconcentration and removal of trace amounts of Hg (II) ion in aqueous samples with nanotubes functionalized carboxyl are displayed. The analysis of Hg2+ ions concentration in water solutions was accomplished by flame atomic absorption spectroscopy. Some parameters including the pH, value, and type of desorption eluent solvent, extraction time, ions effect etc. were optimized. The Freundlich, Langmuir, Temkin, and Dubinin–Radushkevich models were employed to estimate Hg (II) ions adsorption. The Freundlich model was obtained from the study of isotherm studies. The kinetic studies demonstrate the adsorption of Hg (II) ions by adsorbent fitted with the pseudo-second-order type and resulting from that chemisorption is the rate-limiting process in the Hg (II) adsorption. The concentration parameter such as level of detection of proposed method, and relative standard deviation test were obtained as 20.83, 6 µg L−1, and 1.16%, respectively.

Determination of in situ hydrocarbon contents in shale oil plays. Part 2: Two-dimensional nuclear magnetic resonance (2D NMR) as a potential approach to characterize preserved cores

Determination of the in situ hydrocarbon content and occurrence characteristics of shale oil reservoirs is crucial for reserve prediction and efficient development. Although many scholars have studied the fluid saturation of conventional coring shale using solvent and thermal extraction methods, with time consuming and only volumes or mass of oil and water were given, which could not reveal the occurrence characteristics of oil and water in samples. This study proposes a two-dimensional (2D) nuclear magnetic resonance (NMR) T1–T2 mapping method to systematically assess the in situ water and oil content, distribution, and their evaporative loss laws of preserved shales. Sixty preserved shales with different maturities were selected from the first member of the Qingshankou Formation in the northern Songliao Basin, China. Each preserved sample was divided into two parts and analyzed using a high-frequency (22 MHz) NMR and Dean–Stark extraction respectively. Furthermore, a time-elapsed NMR T1–T2 mapping was performed on a preserved shale core sample after varying the exposure times in an open environment. Results show that T1–T2 mapping can be used to determine the porosity, water and oil contents, and saturations of preserved shales. Oil content measured using T1–T2 mapping was consistent with that obtained by Dean–Stark extraction, with a correlation coefficient (R2) as high as 90% when crude oils of varying maturities were used for NMR calibration.The T1/T2 of oil inpreserved shale showed a negative trend with maturity, and the T2 of oil was often higher than that of water, indicating oil is enriched in the relative macropores. After exposing the preserved shale to open air for 150–200 h, 1) the total loss ratio of oil and water was approximately 80%; 2)the fluid evaporation loss rate follows the orders: oil > water and fluids in larger pores > smaller pores; 3)the free oil content decreased by approximately 92%, whereas the absorbed oil content varied slightly. As a nondestructive, rapid, and minimal sample preparation method, 2D NMR can efficiently assess both fluids content and distribution of a core sample in only 5 min, thus displaying its great advantages and applications for preserved shales with fast fluid evaporation to determine the in situ fluid content.

A Fast Method for the Simultaneous Analysis of 26 Beta-Agonists in Swine Muscle with a Multi-Functional Filter by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

A rapid and simplified sample preparation method was developed for the simultaneous determination of 26 beta-agonists in swine muscle using a multi-functional filter (MFF) based on quick, easy, cheap, effective, rugged, and safe methods (QuEChERS). MFF integrated the cleanup and filter procedures, thereby significantly improving the efficiency of sample preparation compared with traditional solid-phase extraction. The sample was processed via enzymatic hydrolysis, purified with the optimized MFF containing 150 mg magnesium sulfate, 50 mg PSA, and 50 mg C18, and then analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry. All procedures can be completed in 6.5 h. Good linearity (R2 &gt; 0.99) was detected in all analytes. The recoveries ranged from 71.2% to 118.6%, with relative standard deviations (RSDs) of less than 18.37% in all spiked concentrations. The limits of detection (LOD) and the limits of quantitation (LOQ) were 0.01–0.10 and 0.10–0.50 μg/kg, respectively. The decision limit (CCα) and detection capacity (CCβ) values fluctuated in the range of 3.44–25.71 and 6.38–51.21 μg/kg, respectively. This method is a good alternative for detecting beta-agonist residues in swine muscle and can be successfully applied to the national risk monitoring of agro-product quality and safety in China.

Microwave-Assisted Acid Digestion of Malaysian Monazite for Determination of REEs Using ICP-MS

The objective of this study to investigate microwave-assisted digestion as a rapid sample preparation method for the determination of REEs in Malaysian monazite using Inductively Coupled plasma-mass spectrometry (ICP-MS). Finely powdered monazite (D90 &lt; 75μm) was the raw material for the digestion and fusion procedures. In the Li-borate fusion method, the digestion was achieved by lithium tetraborate: metaborate flux (Li2B4O7: LiBO2) flux fusion followed by acid attack, using nitric acid (HNO3). The second method, the microwave-assisted digestion method, involved digestion of the monazite in a mixture of H2SO4, HNO3, and HF, followed by neutralization of the insoluble fluorides and complexation of residual HF. The concentrations of REEs, measured by both the methods, were in agreement with each other, except for the values of P and Si, which were slightly apart. Both the sample dissolution methods offer feasible means of quantifying REEs in the monazite sample, but only a combined microwave digestion-fusion technique yields complete quantitative data for monazite-type samples.

Rapid sample preparation workflow based on enzymatic nanoreactors for potential serum biomarker discovery in pancreatic cancer

Mass spectrometry (MS)-based proteomics have been extensively applied in clinical practice to discover potential protein and peptide biomarkers. However, the traditional sample pretreatment workflow remains labor-intensive and time-consuming, which limits the application of MS-based proteomic biomarker discovery studies in a high throughput manner. In the current work, we improved the previously reported procedure of the simple and rapid sample preparation methods (RSP) by introducing macroporous ordered siliceous foams (MOSF), namely RSP-MOSF. With the aid of MOSF, we further reduced the digestion time to 10 min, facilitating the whole sample handling process within 30 min. Combining with 30 min direct data independent acquisition (DIA) of LC-MS/MS, we accomplished a serum sample analysis in 1 h. Comparing with the RSP method, the performance of protein and peptide identification, quantitation, as well as the reproducibility of RSP-MOSF is comparable or even outperformed the RSP method. We further applied this workflow to analyze serum samples for potential candidate biomarker discovery of pancreatic cancer. Overall, 576 serum proteins were detected with 41 proteins significantly changed, which could serve as potential biomarkers for pancreatic cancer. Additionally, we evaluated the performance of RSP-MOSF method in a 96-well plate format which demonstrated an excellent reproducibility of the analysis. These results indicated that RSP-MOSF method had the potential to be applied on an automatic platform for further scaled analysis.

Withdrawal Interval Estimation of Doxycycline in Yellow Catfish (Pelteobagrus fulvidraco) Using an LC-MS/MS Method Based upon QuEChERS Sampling Preparation.

Limited information on residue depletion of doxycycline (DC) is available in yellow catfish (Pelteobagrus fulvidraco) using the determination of LC-MS/MS based upon a rapid and simple method of sample preparation. This study collected plasma and tissue samples of yellow catfish at pre-determined time points following 3-day consecutive oral administration of DC at 20 mg/kg. The samples were prepared using a QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) approach. The limit of detection and the limit of quantitation were 2.5 and 5 µg/kg or µg/L, respectively, for DC in plasma and tissues (e.g., muscle + skin, liver, kidney, and gill). The recoveries of DC ranged from 67.2% to 86.2%. The decision limit (CCα) and the detection capability (CCβ) were from 106.2 to 127.8 μg/kg or μg/L. The withdrawal times of DC in muscle + skin were estimated to be 22 d based on the guidelines in China and Europe and 27 d based on Japan’s standard. Overall, this study not only provides an efficient method to rapidly determine the DC concentrations in fish-derived tissues but also provides important information on the safety assessment of DC in aquatic animal-derived food products.