Rapeseed Oil Fatty Acids Research Articles

Biodegradable lubricants — Analysis of metalworking oils and hydraulic fluids by mass spectroscopy

AbstractBiodegradable metalworking oils and hydraulic fluids were screened for their base oil composition using a special mass spectrometric technique — direct exposure probe chemical ionisation mass spectrometry (CI(NH3)‐MS). All but one of the hydraulic fluid samples were rapeseed oil or trimethylolpropane trioleate based, the other sample being a neopentylglycol ester trimethylolpropane ester mixture. Ethylhexyl ester of rapeseed oil fatty acids (or similar) was present in some of the metalworking fluids in addition to rapeseed oil, whilst other samples were pure rapeseed oil. In several cases a trimethylolpropane/neopentylglycol ester base was found. Preliminary results for additive isolation/identification show the usefulness of preparative gel permeation chromatography.

Serum Levels, Absorption Efficiency, Faecal Elimination and Synthesis of Cholesterol during Increasing Doses of Dietary Sitostanol Esters in Hypercholesterolaemic Subjects

1. Serum cholesterol reduction and changes in cholesterol metabolism were studied during rapeseed oil feeding without and with increasing amounts of sitostanol trans-esterified with rapeseed oil fatty acids and dissolved in rapeseed oil mayonnaise. Fifteen mildly hypercholesterolaemic subjects replaced 50 g of their usual dietary fat by 50 g of rapeseed oil fat mayonnaise for 6 weeks followed by randomization so that eight subjects continued on rapeseed oil mayonnaise alone (control group) for 15 weeks and seven on rapeseed oil mayonnaise with a small dose of sitostanol ester (800 mg/day of sitostanol) for 9 weeks followed by 6 weeks with higher dose of sitostanol ester (2000 mg/day of sitostanol). 2. During the rapeseed oil period the reduction in serum low-density lipoprotein cholesterol was 14% from the home diet. The control-adjusted reduction by the low sitostanol ester dose was 7.4% (not significant) and by the higher dose it was 15.7%. 3. The low dose of sitostanol ester had no consistent effect on cholesterol precursors or cholestanol in serum, reduced serum levels of campesterol and sitosterol by 28.2% and 23.6%, respectively, and reduced cholesterol absorption efficiency significantly from 28.7% to 23.4%. In accordance, faecal excretion of neutral and particularly endogenous neutral sterols increased (16.7% and 19.7%, respectively), but faecal cholesterol elimination and cholesterol synthesis were only insignificantly increased. 4. During the high dose of sitostanol ester the high-density lipoprotein-to low-density lipoprotein-cholesterol ratio increased.(ABSTRACT TRUNCATED AT 250 WORDS)

Serum cholesterol, cholesterol precursors, and plant sterols in hypercholesterolemic subjects with different apoE phenotypes during dietary sitostanol ester treatment

A randomized double-blind study was made in 67 modestly hypercholesterolemic subjects by replacing 50 g of daily dietary fat by the same amount of a rapeseed oil preparation without and with fat-soluble sitostanol esters. The diet became relatively rich in dietary fat (37%) especially in subjects with a low basal calorie intake. The esters were prepared by transesterification of sitostanol with rapeseed oil fatty acids. The effects of sitostanol esters were studied on serum cholesterol and cholesterol synthesis (measuring cholesterol precursors in serum) and absorption (measuring serum plant sterols). The results were related to different apoE phenotypes. A 6-week regimen of about 3.4 g/day of sitostanol lowered total and low density lipoprotein (LDL) cholesterol levels by 7.5% and 10%, respectively, over that due to rapeseed oil alone. High density lipoprotein (HDL) cholesterol and triglyceride concentrations were unchanged. Thus, the HDL/LDL cholesterol ratio was significantly increased. The decrease in LDL cholesterol level was more consistent in subjects with the epsilon 4 allele than in those with homozygous epsilon 3 alleles. Sitostanol markedly decreased serum campesterol (-46%) and sitosterol (-30%), especially in subjects with the epsilon 4 alleles known to have high cholesterol absorption . The decreases of LDL cholesterol and plant sterols were interrelated, suggesting that reduced cholesterol absorption contributed to the lowering of LDL cholesterol. Serum sitostanol was unchanged, while the serum cholesterol precursors, delta 8-cholestenol, desmosterol, and lathosterol, were compensatorily increased by 10% (P < 0.05), most consistently in the subjects with epsilon 4 alleles, indicating an increase in cholesterol synthesis. The study demonstrates that sitostanol esters dissolved in dietary fat can be recommended for treatment of modest primary hypercholesterolemia and are apparently practical and suitable for cholesterol lowering in a general population.

Ruminal lipid balance and intestinal digestion by dairy cows fed calcium salts of rapeseed oil fatty acids or rapeseed oil.

The effects of supplemental calcium salts of rapeseed oil fatty acids (FA) and rapeseed oil on ruminal metabolism and apparent digestibility of lipids in the small intestine were studied using three multiparous Holstein x Friesian cows in a 3 x 3 Latin square design. Cows fitted with ruminal, duodenal, and ileal cannulas were fed restricted amounts of a control diet (C) containing 65% corn silage and 35% concentrate mix or diet C with supplemental calcium salts of rapeseed oil FA (S) or diet C supplemented with rapeseed oil (O). Fatty acid contents were 1.9, 8.4, and 7.6% for diets C, S, and O, respectively. The average flow of total FA to the duodenum was lower than the intake for supplemented treatments, suggesting catabolism of FA by ruminal microbes. Fatty acid flows at the duodenum were higher (P < .10) for diets supplemented with fat than for diet C, except for C18: 3(n-3) and straight-chain and branched-chain C15 and C17. Fat treatment affected total and individual FA flow to the ileum, except C14:0 and C18:2(n-6) and excreted amount of individual FA, except C14:0, C16:0, and total C18:1. In our trial, the addition of fat, regardless of origin, affected small intestinal digestibilities of C14:0 and C18:2(n-6) and did not affect changes in the amount of FA in the hindgut.

Organic matter and nitrogen digestion by dairy cows fed calcium salts of rapeseed oil fatty acids or rapeseed oil1

The aim of this experiment was to study the effects on digestion of a supply of Ca salts of vegetable oil as a fat source for ruminants. Total tract digestibility and ruminal digestion were studied using three ruminally and duodenally fistulated dairy cows in a Latin square design. Diets compared were a control diet (C) based on corn silage and concentrate, and the C diet supplemented with calcium salts of rapeseed oil (S) or supplemented with rapeseed oil (O). Organic matter digestibility was lower (P < .05) for diet O than for diet C; diet S was intermediate and not different (P > .05) from diet C. Treatment did not affect (P > .05) the proportion of OM digested in the rumen, total N and microbial N flow at the duodenum, or efficiency of N microbial synthesis. In situ degradation of DM and NDF was higher for diet S than for diet C. The molar concentration of ruminal acetate was lower (P < .05) for O than for C and S. Under the conditions of this trial, supplemental calcium salts rich in polyunsaturated fatty acids did not decrease ruminal digestion.

Kinetics and balance of a fermentation free from product inhibition: sophorose lipid production by Candida bombicola

The production of sophorose lipids from ethyl esters of rapeseed oil fatty acids and glucose by Candida bombicola CBS 6009 was studied. A kinetic description of the fermentation is presented, showing in particular two distinct phases of growth and, following nitrogen limitation, subsequent production of sophorose lipids, which were excluded from the aqueous phase. With the fed-batch fermentation technology used, a high production performance (320 g·l−1 sophorose lipids with a weight yield of 65% with respect to the carbon sources) was obtained. Detailed fermentation balances were established and their implications for energy metabolism during glycolipid production are discussed. The variations in the structure of the sophorose lipids during fermentation were also studied. Sophorolipid 1′, 4″-lactone 6′, 6″-diacetate was the major class compound produced (about 50% of the glycolipids). A correlation between the lipidic composition of the products and the nature of the fatty acids of the rapeseed esters was observed.

Intestinal absorption of menhaden and rapeseed oils and their fatty acid methyl and ethyl esters in the rat

The relative cellular uptake and incorporation into prechylomicrons and chylomicrons was investigated for the menhaden and rapeseed oil fatty acids, when given by stomach tube as the original oils or the corresponding methyl and ethyl esters. The intermediates and final products of cellular acylation were determined by chromatographic methods at various times over a period of 1-24 h. There was little selectivity in the uptake among the oligo- and poly-unsaturated fatty acids of menhaden oil, when either oil or esters were fed. In contrast, the long-chain saturated and monounsaturated fatty acids of rapeseed oil were discriminated against during both cellular uptake and reacylation (60% overall reduction in utilization). Also, there was detectable discrimination against the long-chain polyunsaturated monoacylglycerols of menhaden oil and against the long-chain saturated and monounsatured monoacylglycerols of rapeseed oil during both cellular uptake and reacylation (30% overall reduction in utilization). Evidence was obtained for an indiscriminate cellular uptake of variable amounts (4-22%) of intact dietary methyl and ethyl esters of fatty acids, which, however, appeared in the chylomicrons only to a very limited extent (0.1-1.0% of total lipid). During peak absorption the cellular and lymphatic appearance of fatty acids from the digestion and absorption of the alkyl esters was nearly 50% lower than that from the corresponding triacylglycerols. The slower absorption of the fatty acids from the alkyl ester feeding is hypothetically attributed to a lower efficiency of the phosphatidic acid pathway, which is required in the absence of dietary 2-monoacylglycerols, but other mechanisms cannot be excluded.

Performance Test of Water Base Lubricants

We have reported that the soap aqueous solution had least frictional coefficient (μ) and the highest-seizure load (ρ) among the various surfactants (Anionics, Cationics, Nonionics & Amphoterics) in J. Jpn. Soc. Lubrication Eng. 29, 839 (1984). The μ and ρ were measured by Soda's 4-b all testing machine under the following condition. (200 r.p.m., step loading velocity 0.5kg/cm2/min, at room temp.) 1% soap solution had less and higher ρ than standard hydrocarbon oil, (100SN, 150SN, 350SN & 600SN) The standard hydrocarbon oil plus 1% of rapeseed oil fatty acids or castor oil fatty acids had the same μ and ρ of 1% soap aqueous solution.