Sound spectrographic analysis demonstrated that the two mating call types of the nominal species Hyla versicolor (fastpulsed and slow-pulsed) are distributed in a definite geographic pattern in the eastern half of the United States (Blair, 1958a). Crosses between the call types, and backcrosses of F1 progeny to the parental types, exhibit a high degree of mortality compared with within call type crosses, whether from sympatric populations, from populations in near allopatry, or from widely allopatric populations (Johnson, 1959, 1963; Ralin, 1976a). Females choose their own call type in discrimination tests (Littlejohn et al., 1960). There are statistically significant but overlapping differences between the two call types in some mensural characters (Johnson, 1961, 1966; Ralin, 1968). The name H. versicolor now represents the slow-call species, the subspecific name chrysoscelis has been elevated to species rank to represent the fast-call species, and the three formerly described subspecies are considered to be invalid (Johnson, 1966). Sound spectrograms and oscilloscope tracings of the mating calls of the two species were presented by Pierce and Ralin (1972). When uncorrected for temperature, the range of pulse rates in H. versicolor (17-35 pulses/s) and H. chrysoscelis (34-69 pulses/s) overlap slightly (Johnson, 1966). However, when corrected for the effects of temperature, there is no overlap in central Texas (Ralin, 1968) or Del-Mar-Va peninsula and New Jersey (Zweifel, 1970) populations of the two species. Karyotypic analysis demonstrated that H. versicolor from northern New Jersey is a sexually reproducing tetraploid population with 4N = 48 chromosomes (Wasserman, 1970). Electrophoretic comparisons of polymorphic loci in populations of H. versicolor from central Texas and populations of H. chrysoscelis from central Texas and east of the Mississippi River indicated that H. versicolor is also tetraploid in Texas (reported by Ralin et al., at Austin, Texas Meeting of Soc. Study of Evolution, 1970; Ralin and Selander, 1977). In every instance of cytological examination, individuals identified by call as H. versicolor proved to be tetraploid, and individuals identified by call as H. chrysoscelis proved to be diploid (Bogart and Wasserman, 1972; Bogart, pers. comm.). Electrophoretic analysis of the Ldb (lactate dehydrogenase-B or heart LDH) locus also indicated that central Texas (western) populations of H. chrysoscelis are genetically differentiated from eastern populations (South Carolina, Georgia, Ohio, and Mississippi), and that both groups of populations apparently have contributed alleles to the formation of Texas H. versi-color (Ralin, 1976b; Ralin and Selander,, 1977). Recent evidence indicates that. males of H. chrysoscelis from near Savan-nah, Georgia have significantly lowerpulse rates and significantly longer call durations than males of H. chrysoscelis from central Texas, and that females from the Savannah area prefer the call of a male from the same population to a central Texas call (Gerhardt, 1974b). An analysis of mating call along northern and southern transects through the ranges of the two call types led to the suggestion by Blair (1958a) that there might be more than two forms involved in this complex. However, correction for the effects of temperature