Excellent Range Research Articles

High-efficiency 5-watt wavelength-tunable UV output from an Alexandrite laser

We investigate the performance of continuous-wave wavelength-tunable ultraviolet output from a diode-pumped Alexandrite laser by intra-cavity second harmonic generation. At 385 nm, ultraviolet output power of 5.05 W is achieved which is the highest continuous-wave ultraviolet power to date for intra-cavity frequency doubled diode-pumped Alexandrite lasers and highest optical-to-optical efficiency of 16.2% is achieved with respect to absorbed red pump power. An excellent ultraviolet wavelength tunable range, 38 nm, is achieved from 364 nm to 402 nm. Cavity mode analysis is performed for design of the resonator including cavity analysis and the modelling of output power from this wavelength tunable ultraviolet laser.

Development and Validation of a Method for Detecting and Quantifying Mitragynine in Kratom Samples Using HPLC-PDA

Kratom (Mitragyna speciosa Korth) has been identified as a New Psychoactive Substance (NPS) by the United Nations Office on Drugs and Crime (UNODC) and included in the list of prohibited ingredients in food supplements and traditional medicine by Indonesian FDA. Therefore, a rapid, easy, and reliable analytical method is necessary to detect and quantify this plant and its products. This study developed a method for the detection and quantification of kratom products based on a unique compound, mitragynine, as a biomarker. A previous survey of determining mitragynine in Kratom using GC-MS, LC-MS/MS, UPLC, and HPLC-PDA. Previously, the HPLC-PDA method used a C8 column. Yet, for efficiency, it is also necessary to develop a test method using a C18 column. Analysis was performed using an HPLC - PDA system with Waters Atlantis T3-C18 (250 x 4.6 mm, 5 µm) column. The mobile phase comprises acetonitrile and formic acid 0.05%, pH 5.0 (75:25 v/v), delivered at a 1.0 mL/min flow rate. Detection was carried out at a wavelength of 225 nm. The analytical method was validated with test parameters of selectivity, system suitability, accuracy, precision, linearity, detection limit, and quantification limit. The validation study demonstrated an excellent linear concentration range of 1.96 - 6.01 µg/mL with a correlation coefficient of 0.9996; the detection limit is 0.14 µg/mL, while the limit of quantification is 0.45 µg/mL—accuracy method of 98.88 - 101.44% and a bias of 0.27%. The percent relative standard deviation for six independent assay determinations was 0.67%, and the intermediate precision was 1.56% on two days. The mitragynine amounts in these materials ranged from 6.01 to 6.31 mg/g of dried leaf material. Based on the research results, it can be concluded that the method developed provides quick, simple, reliable, accurate, and valid, and has an advantage over existing methods in terms of simplicity of sample preparation, short analysis time, and cost-effectiveness compared to GCMS and LCMS/MS and can be applied for future analysis in Kratom samples.

Sensitive and Selective Determination of Benzidine by Synthesized tragacanth-poly (Acrylic acid-co-acrylamide-GQD) Hydrogel Nanocomposite as a Highly Stable Fluorescent Probe.

Benzidine is known as a toxic and highly carcinogenic substance, so its determination is an essential issue. Until now, no effective and stable fluorescent probe based on hydrogel nanocomposite has been reported for the determination of this substance. In this work, for the first time, the synthesis and use of tragacanth-poly (acrylic acid-co-acrylamide-GQD) hydrogel nanocomposite (H-GQD) as a novel, high-stable, and selective fluorescence hydrogel nanocomposite for the identification of benzidine is reported. To achieve the maximum responsiveness of this hydrogel nanocomposite to determine benzidine, various parameters such as pH, ionic strength, hydrogel nanocomposite concentration, sensitivity, and selectivity were investigated. The results of the investigations showed that the synthesized H-GQD has excellent stability, selectivity, and linearity range of 0.3 - 12 ppm with a limit of detection of 0.098 ppm. The results of the investigation of real water samples showed that the H-GQD has excellent recovery in the range of 93.3 - 106.6%. Finally, we believe that this H-GQD as a new and highly stable fluorescent probe can be a starting point for its application in various fields and industries to identify benzidine in water samples.

Impact of Different Drip Emitter Types on Hydraulic Performance and Wetted Zone Characteristics in Okra Cultivation

Drip irrigation system is the most efficient and economical method for irrigation vegetable production. The study aimed to design and evaluate the hydraulic performance of three different types of emitter namely Regular gauge (RG), Compensating pressure (CP) and non-compensating pressure (NCP) using okra as a test of a crop. This study was conducted at the experimental farm of the Faculty of Agricultural Science, Zamzam University in 2023. The treatments were laid out in a randomized complete block design (RCBD) with three replications. Parameters of hydraulic performance of drip emitters were average discharge (Qavg %), discharge variation (Qvar), coefficient uniformity (CU %), coefficient of manufacture variation (CV), emission uniformity (EU %) and statistical uniformity (US %). The results showed that the values of (CU%), (CV), (EU%), (US%) and percentage emitters clogging (Pclog%) were 99.52%, 0.49, 68.01%, 74.14%, 2.8% and 99.32%, 0.36, 52.06%, 63.47%, 1.65% and 99.09%, 0.26, 40.07%, 51.05%, 0.85% for non-compensating pressure (NCP), compensating pressure (CP) and regular gauge (RG respectively. It is considered coefficient uniformity (CU %), was good and found to be within the excellent range while discharge variation (Qvar) was found to be within the desirable range, emission uniformity (EU %), coefficient of manufacture variation (CV), and statistical uniformity (Us %) were found to be within the range of poor, low, acceptable and unacceptable. Thus, the study recommended the best type of emitter was regular gauge (RG), because it has the highest crop yield and water productivity as compared to other emitters, non-compensating pressure emitters (NCP) and compensating pressure emitters (CP)

A validated high-performance liquid chromatography-ultraviolet method for the determination of valproic acid derivatives in pharmaceutical formulations with a microbiological suitability evaluation

Valproic acid and its derivatives are common drugs used in the treatment of epilepsy, a result of CNS disorders. Because the molecular structure of valproic acid, as a fatty acid, does not contain any chromophore groups, its peak response demanded special conditions to be detected in HPLC, ensuring the best precision results. Still, the current approach illustrates simple, validated procedures for conduction. The conducted chromatographic system consists of the BDS Hypersil C8, 150 × 4.6 mm, 5 µm column using a mobile phase of acetonitrile: phosphate buffer (4:6) at a detection wavelength of 215 nm at room temperature. A full method validation study was conducted and approved to ensure precise, repeatable, and accurate results by implementing system suitability parameters. In microbiological terms, a suitability test is used to validate microbiological testing methods, ensuring their efficacy despite potential interference from antimicrobial properties in the tested materials. The HPLC-UV developed and validated analytical method was evaluated, and it was found to be sensitive for use in the detection of low concentrations of methylparaben, propylparaben, and valproate with an optimum run time of six minutes. limit of detection (LODs) were statistically estimated and found to be 2.27 µg/mL, 39.77 ng/mL, and 1.84 µg/mL for methylparaben, propylparaben, and valproate, respectively. Additionally, the method demonstrated a high recovery of methylparaben, propylparaben, and valproate with an excellent closed-accuracy range (98.84% - 101.34%). An excellent regression coefficient (r) of 0.99924, 0.99998, and 0.99997 for methylparaben, valproate, and propylparaben, respectively, was achieved. Assay determination of various pharmaceutical dosage forms in the local market was implemented, yielding admirable results.

Novel electrochemical sensor based on poly([Cu(H2O)2P2]I2)/GCE for the determination of trimethoprim in clinical samples and cow's milk samples

A Novel bioanalytical detector based on Poly(Diaquabis(1,10-phenanthroline copper (II) Iodide) (poly([Cu(H2O)2P2]I2)/GCE) has been fabricated by potentiodynamic polymerization of [Cu(H2O)2P2]I2. The synthesized complex and the fabricated electrodes showed a promising electrocatalytic behavior towards the electrooxidation of Trimethoprim (TPM). Based on the regression coefficient value of scan rate with peak current and square root of peak current, the oxidation of TMP at poly([Cu(H2O)2P2]I2)/GCE was dominated by diffusion. Under the optimized experimental conditions and square wave voltammetric parameters, the poly([Cu(H2O)2P2]I2)/GCE revealed very wide responses in the range of concentration of 500 nM–160 μM with a limit of detection of 3.91 nM and a Limit of quantification of 13.02 nM with the relative standard deviation below 1.75 %. The valuable applicability of the fabricated sensor for the determination of TMP in real samples, including cow's milk, blood serum, and human urine, was successfully investigated. The electroanalysis results from the spike recovery and interference study provided an excellent range of recovery with an error percent of below 3.0 % in the combination effect with the potential interferents. The sensor exhibited excellent reproducibility, sensitivity, long-term stability, high accuracy, and fast response. The developed bioanalytical sensor revealed the best application prospect for biomedicine and environmental monitoring activities.

Methods established for the detection of mineral levels in whole blood by the dried blood spot technique

Mineral are intimately related to human health and disease, and detection of mineral content in the body is of great significance for the diagnosis and prevention of diseases. In this study, we validated the method developed to detect magnesium (Mg), copper (Cu), iron (Fe), zinc (Zn), and selenium (Se) levels in dried blood spots (DBS). In accordance with the requirements of the guidelines for the Bioanalytical Method Validation Guidance for Industry, we evaluate the linearity, sensitivity, precision, accuracy and selectivity of the developed methods. In addition, Mg, Cu, Fe, Zn and Se were quantified in 195 older adults using DBS technique, and its accuracy was assessed by comparing the results to those detected by inductively coupled plasma-mass spectrometry (ICP-MS). The method has excellent sensitivity and linear range to cover the concentration range of mineral elements in the general population with the required precision, accuracy and selectivity. The correlation coefficients of Mg, Cu, Fe, Zn and Se levels in blood detected by the DBS technique and ICP-MS were 0.638, 0.823, 0.463, 0.728 and 0.751, respectively (all P < 0.05), which indicated that there was a strong correlation between the detection results of the two methods. More than 95 % of the sample results in the Bland-Altman consistency test were within the acceptable limits of agreement (LOA) range, indicating that they had good consistency. DBS technique has good accuracy and reliability in detecting blood mineral levels in the elderly, suggesting potential in the quantification of mineral level in blood.

Microscale cephalosporin ion-selective amperometric/voltammetric sensors based on a micropipette-supported liquid/liquid interface: Multiple purposes of transmembrane studies and real-time determinations

The permeability of cephalosporins through cell membranes is crucial for their efficacy against gram-negative bacteria. To address this, a microsoft biphasic sensor utilizing the interface between two immiscible electrolyte solutions (ITIES) has been introduced for studying the ion transfer of cefotiam (CTM), ceftazidime (CAZ) and cefepime (CPM). The typical voltammograms of cephalosporin ion transfer can be further used to determine the formal ion transfer potentials (∆owφA′), ion transfer Gibbs free energies (∆owGA′), effective hydrophilicity (logPi), geometry of micro-ITIES, diffusion coefficient (Dw) and ion transfer rate constant (k0). In addition, the partitioning of CTM+, CAZ+, and CPM+ in two phases at different pH values was revealed by the corresponding ion partition diagrams (IPDs). The IPDs can further elucidate the mechanism of cephalosporin ion transfer across the ITIES at different pH values. Finally, the determination of CTM+, CAZ+ and CPM+ was achieved via real-time chronoamperometry. An excellent linear range for cephalosporin ions with a satisfactory limit of detection was obtained. The findings show the superb selectivity and anti-interferent ability of micro-ITIES sensors toward cephalosporins. The constructed micro-ITIES sensors for cephalosporin could contribute to medicinal design, single microorganism metabolism analysis, clinical diagnosis, and food safety inspection.

Instrumental activities of daily living in neuro-oncology: International validation of the EORTC IADL-BN32 questionnaire

BackgroundNeurocognitive impairments are common in patients with a brain tumour, and may negatively impact on functioning in daily life, particularly on instrumental activities of daily living (IADL). The EORTC IADL-BN32 questionnaire was developed to measure IADL in this patient population. MethodsIn this international validation study, we evaluated the EORTC IADL-BN32 questionnaire on several psychometric properties in a large sample of patients with a primary or metastatic brain tumour. We administered the 32-item questionnaire three times: at ‘baseline’, after 2 weeks and after 3 months. Procedures were in accordance with EORTC Quality of Life Group module development guidelines. ResultsIn total, 326 patients participated in the study. A bifactor scale structure showed satisfactory model fit measures, with five multi-item scales and two single items, and an IADL sum score. The internal consistency of the multi-item scales ranged from good to excellent (range Cronbach’s α: 0.86–0.97). We found significant differences in scale scores between patients with and without neurocognitive impairments or complaints, supporting the construct validity. Initial cross-cultural validity analyses showed indications of item response biases for certain items. Analyses indicated moderate to good test-retest agreement (intraclass correlation coefficient > 0.70) between baseline and the 2-week follow-up assessment for all but one scale. Deterioration of EORTC IADL-BN32 scale scores were consistent with clinically relevant deterioration on other functional measures with small to large effect sizes, however, subgroup sample sizes were small. ConclusionOverall, the EORTC IADL-BN32 questionnaire exhibited adequate to excellent psychometric properties. Cross-cultural validity and responsiveness should be further explored.

Fe-doped WO3-Modified sensor for the improved electrochemical detection of curcumin

In this study, an iron-doped tungsten oxide (Fe-WO3) nanomaterialwas synthesizedand utilized to modify carbon paste electrodes for the identification of the bioactive ingredient curcumin (CUM). Turmeric contains a bioactive compound called curcumin, which is yellow and has various potential biological applications, such as being an anti-carcinogenic, anti-inflammatory, and antioxidant agent. The Fe-WO3 nanomaterial underwent characterization through SEM, XRD, and EDS techniques. The electrochemical performance of CUM was inquired using square wave and cyclic voltammetry methods. During catalytic oxidation, the Fe-WO3 working electrode assembly showed faster electron transport than the bare CPE. Investigations havebeen conductedinto the kinetics of oxidation, and the developed sensor demonstrated an excellent linear detection range (5 μM to 60 μM), a decreased limit of detection (6.2 × 10−8 M), and a limit of quantification under optimal conditions (20.7 × 10−8 M).The numbers of electrons transmitted, the heterogeneous rate constant, the electron transfer coefficient, and the electrochemical oxidation were all measured. The results from the examination of CUM in actual samples using the devised Fe-doped WO3 modified electrode were good. Additionally, it showed a considerable increase in the curcumin peak current concerning responsively, specificity, and reliability for curcumin analysis.

Carbon dots-based dual-mode sensor for highly selective detection of nitrite in food substrates through diazo coupling reaction

As an antioxidant and preservative agent, nitrite (NO2−) plays an essential role in the food industry to maintain freshness or inhibit microbial growth. However, excessive addition of NO2− is detrimental to health, so accurate and portable detection of NO2− is critical for food quality control. Notably, the selectivity of most carbon dots (CDs)-based fluorescence sensors was not enough due to the nonspecific interaction mechanism of hydrogen bond, electrostatic interaction and inner filter effect etc. Herein, a novel fluorescence/UV–vis absorption (FL/UV–vis) dual-mode sensor was developed on basis of mC-CDs, which were prepared by simple solvothermal treatment of m-Phenylenediamine (m-PDA) and cyanidin cation (CC). The fluorescence of these mC-CDs could be selectively responded by NO2− through the specific diazo coupling reaction between NO2− and amino groups on the surface of mC-CDs, thus effectively improving the selectivity of NO2− detection. The CDs-based fluorescence sensor possessed a low detection limit of 0.091 μM and 0.143 μM for FL and UV–vis methods and the excellent linear range of 0.0–60.0 μM. Furthermore, the mC-CDs sensor was employed to detect NO2− in real samples with a recovery rate of 97.11 %–104.15 % for quantitative addition. Moreover, the smartphone-assisted fluorescence sensing platform developed could identify the subtle color changes that could not be distinguished by the naked eye, and had the advantages of fast detection speed and intelligence. More importantly, the portable solid phase sensor based on mC-CDs had been successfully applied to the specific fluorescence identification and concentration monitoring of NO2−. Accordingly, the designed sensor provided a new strategy for the highly selective and convenient sensing of NO2− in food substrates, and paved the way for the wide application of CDs-based nanomaterials in the detection of food safety.

A photoelectrochemical sensor based on polydopamine membrane-coated CdS@C core-shell heterostructure for curcumin detection

Curcumin has been widely employed in various fields, but excessive intake may cause tissue necrosis and pose a serious threat to people’s lives and health. Hence, it is crucial to rapidly and precisely detect the content of curcumin. In present research, CdS@C heterostructure materials were successfully prepared through a one-pot solvothermal reaction strategy, utilizing glucose as the carbon source. Simultaneously, polydopamine thin films (ePDA) were successfully deposited on the surface of CdS@C-modified electrodes by the cyclic voltammetry (CV) method. A photoelectrochemical sensor based on the polydopamine membrane-coated CdS@C core–shell heterostructure (ePDA/CdS@C) was designed for curcumin detection. Furthermore, scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and ultraviolet-visible spectrophotometers (UV-vi) techniques were applied to characterize the structure and morphology of the synthesized photoelectric materials. The ePDA coating enhances the stability of the CdS@C material, and the CdS@C core-shell heterostructure accelerates electron transfer. Consequently, the ePDA/CdS@C material generates an outstanding and steady photocurrent response. Under ideal conditions, the novel sensor exhibits a satisfactory linear response to curcumin detection within the concentration range of 0.025–370 μM, with a detection limit as low as 0.0165 μM. Additionally, the ePDA/CdS@C-based photoelectrochemical aptasensor displays remarkable reproducibility, stability, and selectivity. This innovative sensing platform accurately detects curcumin in human serum and urine samples, with an excellent recovery range (99.47 %–103.60 %) and relatively low RSD values (1.49 %–3.56 %). In conclusion, this work offers a novel approach for curcumin detection.

Facile Fabrication of Bio-Nanohybrid Electrode with Guanine/Cytosine-Modified Electrochemically Reduced Graphene Oxide Electrode and Its Application in Doxorubicin Analysis

Graphene, known for its outstanding physical and chemical properties, is widely used in various fields, including electronics and biomedicine. Reduced graphene oxide (rGO) is preferred for electrochemical applications due to its enhanced water solubility and dispersion. Electrochemically reduced graphene oxide (ErGO) is particularly advantageous as it can be prepared under mild conditions and simplifies sensor fabrication; however, ErGO-based electrochemical sensors often lack specificity. Bioreceptors like proteins, enzymes, and DNA/RNA aptamers are incorporated to provide high specificity. This study introduces a guanine (G)/cytosine (C)-modified ErGO electrode (G/C@ErGO-GCE) for the sensitive electrochemical detection of doxorubicin (DOX) with good selectivity. The G/C mixture acts as a bioreceptor and is anchored on the ErGO-GCE surface via π-π interactions. The G/C@ErGO-GCE was characterized using scanning electron microscopy, contact angle measurement, Raman spectroscopy, and electrochemical methods. The sensor demonstrated excellent dynamic range (DPV: 10 nM to 1 µM, CA: 30 nM to 1.3 µM), sensitivity (DPV: 2.17 µA/µM, CA: 6.79 µA/µM), limit of detection (DPV: 84 nM, CA: 34 nM), and selectivity for DOX detection, highlighting its potential for biomedical applications and pharmacokinetic studies.

Communication quality predicts patients’ colorectal cancer screening behavior

The purpose of the present study was to investigate the quality of patient/clinician communication as one potential factor that impacts colorectal cancer screening behavior. As part of a larger randomized controlled trial conducted between 2011 and 2016 in the setting of community and academic family medicine or internal medicine practices in Michigan, USA, patients completed a pre-encounter survey, completed their regularly scheduled visit with their primary care clinician (which was audio-recorded), completed a post-encounter survey, and allowed 6-month follow-up chart audit. We trained 10 coders to rate 216 of the audio-recorded conversations between 216 patients and their primary care physicians for 6 specific features of communication quality (using 7-point scales), including the extent to which participants enacted attention to medical content, engagement, emotional expression, relationships, face, and accommodation. At least 3 coders rated each conversation, and intraclass correlations (i.e., reliability assessment) were in the good to excellent range. We found that patient and clinician attention to face (an identity goal) was a significant predictor of colorectal cancer screening at 6 months follow up. Measuring communication in terms of attention to multiple goals reveals unexpected findings about the aspects of communication that impact colorectal cancer screening behavior. The focus of many interventions to improve colorectal cancer screening rates is on the content (i.e., task goals) of clinicians' communication (such as presenting the different options for screening), yet the content of communication was not a significant predictor of screening in the present study. Rather, clinicians' and patients’ attention to identity goals predicted screening behavior, which suggests that interventions may not need to be overly complex and that simply improving the quality of attention to identity goals in clinician communication might be one of the most straightforward yet impactful ways to improve colorectal cancer screening uptake among patients.

Fabrication of iron manganese oxide-reduced graphene oxide nanocomposite: A highly effective synergistic electrocatalyst for sensitive metronidazole detection

Efficient 2D-inorganic spinel perovskites nanostructure materials are used as potential candidates for highly sensitive detection of harmful substances present in the environment. In this regard, a facile one-pot hydrothermal synthesis was employed to fabricate nanocomposite material containing iron manganese oxide (FeMn2O4) decorated with reduced graphene oxide (rGO) which is used as an electrochemical detection of metronidazole (MTZ). The crystalline nature and structural insights of the as-synthesized FeMn2O4-rGO nanocomposite were established using various spectroscopic and electrochemical techniques. Distinctively FeMn2O4-rGO nanocomposite fashioned glassy carbon electrode (GCE) showed excellent linear range (0.01–512.2 μM), sensitivity (71.4 μA mM−1 cm−2), limit of detection (0.0129 μM), reproducibility, stability and resist-interference capability towards metronidazole (MTZ) antibiotic drug. In addition, the fabricated FeMn2O4-rGO/GCE electrochemical sensor exhibited a remarkable MTZ detection in spiked water as well as urine samples with better recovery percentages supporting its facile and feasible nature for environmental applications. The present work established the fabrication of FeMn2O4-rGO/GCE nanocomposite for effective determination of antibiotic drugs in real sample analysis.

A novel urease-assisted ratiometric fluorescence sensing platform based on pH-modulated copper-quenched near-infrared carbon dots and methyl red-quenched red carbon dots for selective urea monitoring.

A novel and sensitive fluorescence ratiometric method is developed for urea detection based on the pH-sensitive response of two fluorescent carbon dot (CD) systems: R-CDs/methyl red (MR) and NIR-CDs/Cu2+. The sensing mechanism involves breaking down urea using the enzyme urease, releasing ammonia and increasing pH. At higher pH, the fluorescence of NIR-CDs is quenched due to the enhanced interaction with Cu2+, while the fluorescence of R-CDs is restored as the acidic MR converts to its basic form, removing the inner filter effect. The ratiometric signal (F608/F750) of the R-CDs/MR and NIR-CDs/Cu2+ intensities changed in response to the pH induced by urea hydrolysis, enabling selective and sensitive urea detection. Detailed spectroscopic and morphological investigations confirmed the fluorescence probe design and elucidated the sensing mechanism. The method exhibited excellent sensitivity (0.00028 mMLOD) and linearity range (0.001 - 8.0 mM) for urea detection, with successful application in milk samples for monitoring adulteration, demonstrating negligible interference and high recovery levels (96.5% to 101.0%). This ratiometric fluorescence approach offers a robust strategy for selective urea sensing in complicated matrices.

Reconstruction of patella fractures with the tension band technique: A review on clinical results and tips and tricks

IntroductionThe goals of surgical treatment of patellar fractures are a biomechanically stable joint and congruent restoration of the retro patellar joint surface. Surgical treatment strategies for patellar fractures have evolved from tension band in combination with wire cerclages to new devices. MethodsThe modified anterior tension band (MATB) technique for fixation of patellar fractures consists of two longitudinal 1.8 mm Kirschner wires (K-wires) and an 18-gauge stainless steel wire looped in a figure-of-8 pattern over the anterior aspect of the patella. The K-wires should be inserted 5 mm from the anterior cortical surface of the patella, parallel in the coronal and sagittal planes. For mechanical reasons, the wire should be the closest to the anterior aspect of the bone. This construct converts the anterior tensile forces generated by the extensor mechanism and knee flexion into compressive forces on the anterior aspect of the fracture site. The MATB is the most widely accepted method of internal fixation for transverse and comminuted patellar fractures.Only a careful implementation of the MATB in all its phases will provide the best mechanical construct and the least aggressive construct for the soft tissues, allowing early re-education without complications. ResultsGood to excellent clinical results (64–100 %) have been reported with MATB for fixation of patellar fractures. Good to excellent range of knee motion and satisfactory results have been reported despite a high percentage (up to 60 %) of secondary procedures, mainly for removal of symptomatic hardware. ConclusionThis article provides an overview of the use and results of the MATB technique for patellar fractures and the means to improve results with this technique.

Osteosynthesis and Reconstruction in Neglected Neck of Femur Fracture: A single-center Study and literature review.

Purpose : Our study evaluates the effectiveness of our innovative treatment for neglected femoral neck fractures. Methods : This retrospective study included 23 patients from January 2016 to March 2023, treated with our new innovative technique. Data has been extracted from the respective department, and informed consent has been obtained prior to any intervention. Radiographs, including AP and lateral radiographs and range of motion at the hip joint, were evaluated in follow-ups at different intervals. Post-operative complications and total hip arthroplasty conversions were also noted. The SPSS software version 26 was used to analyze data for this study. Results : With a mean follow-up of 4.5 ± 1 years, the participants’ ages ranged from 14 to 60 years old, with a mean age of 29.74 ± 11.47 years. The database record indicates that 20 subjects had the radiological and clinical union observed. The non-union was reported in 3 (14.2%) patients; however, only one was symptomatic. Almost 41.7% of the patients reported feeling pain occasionally after exertion. At the operated hip, all patients had excellent range of motion (ROM), primarily in the areas of hip flexion, abduction, adduction, and external rotation. Following reconstruction, the average hip Haris score was 83.46 ± 10.40 at one year. As a post-operative complication, 4 individuals (16.7%) had an infection. Conclusion : Our unique technique has dramatically improved neglected femur neck fractures, even in young patients. It involves ORIF using three cancellous screws, non-vascularized fibular and iliac crest cancellous grafts for reconstruction, and vascularized Tensor fascia lata pedicle graft osteosynthesis.

Laser-Induced Electrochemical Biosensor Modified with Graphene-Based Ink for Label-Free Detection of Alpha-Fetoprotein and 17β-Estradiol.

In this research, a novel electrochemical biosensor is proposed based on inducing graphene formation on polyimide substrate via laser engraving. Graphene polyaniline (G-PANI) conductive ink was synthesized by planetary mixing and applied to the working zone of the developed sensor to effectively enhance the electrical signals. The laser-induced graphene (LIG) sensor was used to detect alpha-fetoprotein (AFP) and 17β-Estradiol (E2) in the phosphate buffer saline (PBS) buffer and human serum. The electrochemical performance of the biosensor in determining these biomarkers was investigated by differential pulse voltammetry (DPV) and chronoamperometry (CA). In a buffer environment, alpha-fetoprotein (AFP) and 17β-Estradiol detection range were 4-400 ng/mL and 20-400 pg/mL respectively. The experimental results showed a limit of detection (LOD) of 1.15 ng/mL and 0.96 pg/mL for AFP and estrogen, respectively, with an excellent linear range (R2 = 0.98 and 0.99). In addition, the designed sensor was able to detect these two types of biomarkers in human serum successfully. The proposed sensor exhibited excellent reproducibility, repeatability, and good stability (relative standard deviation, RSD = 0.96%, 1.12%, 2.92%, respectively). The electrochemical biosensor proposed herein is easy to prepare and can be successfully used for low-cost, rapid detection of AFP and E2. This approach provides a promising platform for clinical detection and is advantageous to healthcare applications.

Cerium vanadate/functionalized carbon nanofiber composite for the electrochemical detection of nitrite

This study presents a facial and quick electrochemical sensor platform that offers remarkable water and food safety applications. The present work represents a study of the synthesis and characterization for efficient cerium vanadate (CeVO4) with a functionalized carbon nanofiber (f-CNF) decorated electrode, which is a highly effective electrode modifier for sensitive nitrite detection. The CeVO4 nanoparticles were synthesized using the facial hydrothermal technique, and a composite (CeVO4@f-CNF) was prepared using the sonication method. Afterward, the produced materials were confirmed with spectroscopic and microscopic analysis. The electrochemical behavior of nitrite was studied through cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The DPV analysis depicts an excellent linear range of 0.1˗1033 μM and a promising detection limit of 0.004 μM for the proposed electrode. The CeVO4@f-CNF electrode was applied to detect nitrite in water and meat samples. The proposed electrochemical sensor attributes the significant results towards the detection of nitrite.