Randomized Group Design Research Articles

APLIKASI PUPUK VERMIKOMPOS DAN POC URIN SAPI TERHADAP PERTUMBUHAN DAN HASIL TANAMAN TOMAT (Solanum lycopersicum)

This study aimed to determine the effect of vermicompost fertilizer dose and concentration of cow urine Liquid Organic Fertilizer (LOF) on the growth and yield of tomato plants (Solanum lycopersicum). This research was an experimental study conducted from March to May 2024. The experimental design used in this study was a factorial Randomised Group Design (RGD) consisting of two factors. The first factor was the dose of vermicompost fertilizer, which consisted of 4 levels, including 0 g/plant, 35 g/plant, 45 g/plant, and 55 g/plant. The second factor was the cow urine concentration LOF, which consisted of four levels: 0 ml/l, 30 ml/l, 60 ml/l, and 90 ml/l. The results showed that the treatment with a vermicompost fertilizer dose of 45 g/plant had the best effect on the parameters of plant height at 4 weeks (79.16 cm), 5 weeks (94.58 cm), and 6 weeks (117.08 cm), and stem diameter at 4 weeks (6.00 mm), 5 weeks (7.01 mm), and 6 weeks (7.94 mm). The concentration of cow urine LOF at 60 ml/l had the best effect on the parameters of plant height at 4 weeks (78.50 cm), 5 weeks (95.66 cm), and 6 weeks (116.25 cm), the number of fruits per plant (12.83 fruits), and fruit weight per plant (474.75 g). There was an interaction between the treatment of vermicompost fertilizer dose and concentration of cow urine, with a combination of 45 g/plant of vermicompost and 60 ml/l cow urine having the best effect on the parameter of plant height 6 weeks after planting (123.33 cm).

Application of Bacillus sp Liquid Formula Using Root Infusion Technique To Control VSD (Vascular Streak Dieback) Disease In Cocoa

This study aims to explore the potential of liquid organic waste formula made from Bacillus sp to suppress the severity of vascular streak dieback (VSD) disease in cocoa plants. The research was conducted on smallholder cocoa plantations in Patimbe Village, Palolo Subdistrict, Sigi Regency, Central Sulawesi. The research was arranged in a non-factorial Randomised Group Design (RAK) with 5 treatments and 5 replications. The treatments consisted of control (P0=no formula); coconut water + Bacillus sp (P1); rice washing water + Bacillus sp (P2); tofu water + Bacillus sp (P3); and a combination of coconut water, rice washing water, and tofu water + Bacillus sp (P4). Each experimental unit consisted of 10 cocoa plants infected with VSD disease. The liquid organic waste formula was applied using the root infusion technique in months 1 and 3 at a dose of 250 ml/root. Observations of disease symptoms, disease development, disease severity, and disease suppression efficacy were conducted monthly. The results showed that the combination of organic waste of coconut water, tofu water, and rice washing water with Bacillus sp active ingredient was more effective in suppressing VSD disease than liquid organic waste alone with an effectiveness rate of 81.7%.

Potential Combination of Roselle Flower Water Honey and Dates on Blood Pressure in Hypertensive Patients

High blood pressure is a condition where systolic blood pressure is more than 140 mmHg and diastolic blood pressure is more than 90 mmHg. The aim of the study was to determine the effect of giving roselle flower decoction combined with honey and dates on blood pressure in hypertensive patients. The type of research was an experimental study with a randomized group design (RGD) divided into 4 treatment groups: the control group was given a leaflet, treatment group 1 was given 10 g of roselle, treatment group 2 was given 30 g of roselle, and treatment group 3 was given 50 g of roselle. Administered for 7 days with 1 time per day. The number of respondents was 28 people with a purposive sampling technique. The research instrument used a digital blood pressure monitor, 24-hour recall form, statistical analysis used ANOVA and Duncan's further test. The results showed that there were significant differences from the four treatments, with post-systolic p<0.000 and post-diastolic p<0.004, it can be concluded that each treatment group after the intervention had significant differences. The best result was treatment 3 with a roselle dose of 50 g. There is an effect of giving roselle flower decoction combined with honey and dates on blood pressure in hypertensive patients in the working area of the Telaga Dewa Community Health Center, Bengkulu City. Further research is needed on dose modification and duration of administration for maximum BP reduction.

Effectiveness Test of Indigenous Plant Growth Promoting Rhizobacteria (PGPR) Isolate on the Growth of Acehnese Patchouli (Pogostemon cablin Benth.) Sidikalang Variety

Plant Growth Promoting Rhizobacteria (PGPR) is a group of bacteria that live saprophytically in the root area (rhizosphere) acting as a plant growth promoter. The purpose of this study was to test the effectiveness of indigenous rhizobacterial isolates from Aceh Besar and Aceh Tengah in supporting the growth of Aceh patchouli Sidikalang variety. This research was conducted at the Laboratory of Seed Science and Technology, Faculty of Agriculture, Syiah Kuala University and patchouli planting was carried out at the Patchouli Innovation Park (Nino Park), Syiah Kuala University, which took place from December 2022 to June 2023. This study used a non-factorial Randomized Group Design (RAK) with 3 replications. The factor studied was the type of rhizobacterial isolate consisting of 15 treatment levels, namely 1 treatment without rhizobacteria, 7 treatments given indigenous rhizobacterial isolates from Lambada, Seulimeum District, Aceh Besar Regency and 7 treatments given rhizobacterial isolates from Paya Tampu village, Rusip Antara District, Central Aceh Regency. The observation parameters studied are of plant height, number of leaves, number of branches, stem diameter, area per leaf blade, root length, root volume and wet biomass weight of plants. The results showed that indigenous rhizobacterial isolates from Aceh Besar and Aceh Tengah effectively increased the average plant height of Sidikalang variety patchouli at 4 and 8 MSA (26%, and 24%), number of leaves (46%, and 58%), number of branches (78% and 50%), area per leaf blade (34%), root length (19%), root volume (60%) and plant wet biomass weight (50.61%).

A comparison of new cardiovascular endurance test using the 2-minute marching test vs. 6-minute walk test in healthy volunteers: A crossover randomized controlled trial.

This was a 2×2 randomized crossover control trial. To compare the cardiovascular endurance of healthy volunteers using a 2-minute marching test (2MMT) and a 6-minute walk test (6MWT). This study included 254 participants of both sexes, aged 20-50 years, with a height and body mass index (BMI) of ≥150 cm and ≤25 kg/m2, respectively. Participants were hospital staff who could perform activities independently and had normal annual chest radiographs and electrocardiograms. A group-randomized design was used to assign participants to Sequence 1 (AB) or 2 (BA). The tests were conducted over 2 consecutive days, with a 1-day washout period. On day 1, the participants randomly underwent either a 6MWT or 2MMT in a single-anonymized setup, and on day 2, the tests were performed in reverse order. We analyzed maximal oxygen consumption (VO2max) as the primary outcome and heart rate (HR), respiratory rate (RR), blood pressure (BP), oxygen saturation, dyspnea, and leg fatigue as secondary outcomes. Data were collected from 127 participants, categorized into two groups for different testing sequences. The first (AB) and second groups had 63 and 64 participants, respectively. The estimated VO2max was equivalent between both groups. The 2MMT and 6MWT estimated VO2max with a mean of 41.00 ± 3.95 mL/kg/min and 40.65 ± 3.98 mL/kg/min, respectively. The mean difference was -0.35 mL/kg/min (95% confidence interval: -1.09 to 0.38; p <0.001), and no treatment and carryover effect were observed. No significant changes were observed in HR, RR, and systolic BP (p = 0.295, p = 0.361 and p = 0.389, respectively). However, significant changes were found in the ratings of perceived exertion (p <0.001) and leg fatigue scale (p <0.001). The 2MMT is practical, simple, and equivalent to the 6MWT in estimating VO2max. TCTR20220528004 https://www.thaiclinicaltrials.org.

The Effect of NASA Liquid Organic Fertilizer and Chicken Manure on the Growth of Sweet Corn Plants (Zea mays saccharata Sturt)

This research aims to determine the effect of a combination of NASA POC and chicken manure on the growth and production of sweet corn (Zea mays saccharata Sturt). The research used a Randomized Group Design (RAK) with two treatment factors. The first factor is the administration of NASA Liquid Organic Fertilizer (A) consisting of 4 treatment levels, namely: A0 = 0 ml/liter of water (control), A1 = 2 ml/liter of water, A2 = 4 ml/liter of water and A3 = 6 ml /liter of water. The second factor is the provision of chicken manure (B) consisting of 3 treatment levels, namely: B1 = 2.25 kg/plot (7.5 tons/ha), B2 = 4.50 kg/plot (15 tons/ha) and B3 = 6.75 kg/plot (22.5 tons/ha). The results showed that giving NASA POC up to 6 ml/l of water significantly increased plant height, ear length, ear weight per plot and total dissolved solids. Providing chicken manure up to 6.75 kg/plot significantly increased plant height, ear length, weight per plot and total soluble solids. The interaction between NASA POC and chicken manure did not significantly affect plant height, ear length, weight per plot, or total dissolved solids.

Sub-Chronic Toxicity Test of Purple Leaf Extract on the Kidney Histopathology of Male White Rats

Traditional herbal medicine has become increasingly popular as an alternative to conventional medical treatments. Although the plant offers numerous health benefits, caution should be exercised regarding its long-term use. Long-term use of traditional herbal remedies can have detrimental effects on the kidneys. This study was designed to assess the histological changes in the kidneys of male rats following 28 days of purple sage leaf extract administration and to identify potential harmful effects. The study utilized 20 male Wistar rats and utilized a randomized group design for the experimental methodology. Rats were categorized into four distinct groups, consisting of one control group and three treatment groups. The control group received solely sodium chloride, whereas the treatment group 1 (P1) received 500 mg/kg BB of purple sage ethanol extract. Treatment group 2 received up to 2000 mg/kg BB of purple sage ethanol extract, while treatment group 3 received up to 5000 mg/kg BB. The oral examination was conducted for a duration of 28 days. The treatment groups showed an increase in weight and organ index of the right kidney. The findings suggest a potential for toxicity resulting from the administration of purple sage leaf extract over a duration of 28 days.

Effect of Various Biochar Materials and Levels of Chicken Manure Fertilizer on Soil Chemical, Growth and Yield of Soybean (Glycine max L Merrill)

This study aims to determine the effect of various biochar materials and doses of chicken manure fertilizer on soil chemical properties, growth and yield of soybean. The research method used a 2 factorial Randomised Group Design. The first factor was the treatment of various biochar materials consisting of 4 levels, namely: B0 without treatment, B1 (corn cob biochar 1.5 kg/m2, B2 (jengkol bark biochar 1.5 kg/m2), B3 (rice husk biochar 1.5 kg/m2). Factor II was chicken manure (P) consisting of P0 (0 kg/m2), P1 (0.5 kg/m2), P2 (1 kg/m2 and P3 (1.5 kg/m2). The application of various biochar materials and chicken cohe fertilizer gave a real response to soil pH, organic C, total N, available P and K available. The results showed that the application of biochar to the growth and yield of soybean plants gave a significant effect on the parameters of stem diameter, number of productive branches, number of pods, dry weight of seeds/m2, seed production/ha. The application of jengkol skin biochar gave the highest effect on stem diameter (1.750 cm), number of productive branches (5.06 branches), number of pods (181.60 pods), dry weight of seeds/m2 (442.98 grams). The application of chicken manure fertilizer on the growth and yield of soybean plants gives a significant effect on stem diameter, number of productive branches, number of pods, dry weight of seeds/plot. The dose of chicken manure fertilizer 1.5 kg/m2 showed the highest effect on stem diameter (1.79 cm), number of productive branches (5.19 branches), number of pods (184.22 pods), dry weight of seeds/m2 (398.50 g). The interaction of biochar and chicken manure fertilizer did not give significant effect on all treatments. Keywords: Biomass, Biochar, Chicken Manure, Soybean

Persentase penambahan bagian telur ayam pada proses pengolahan amplang ikan lele dumbo (Clarias gariepinus)

Amplang is a traditional food/cracker of East Kalimantan that is made by mixing mackerel, tapioca flour, eggs, and some seasonings. Innovation is needed to improve the local product of african catfish (Clarias gariepinus). The portion of egg has a different functions in the dough of amplang. This study aimed to determine the effect of the portion and percentage of eggs on the chemical, physical, and organoleptic characteristics of amplang made from catfish (Clarias gariepinus). The study used randomized group design (RGD) in factorial with two factors. The first factor (A) was the eggs that were added consisted of egg white, yolk, and mixed egg white and yolk (1:1). The second factor (B) was the percentage of eggs that were added to the weight of african catfish (20%, 30%, 40%). The result showed that the best characteristics of amplang were amplang A1B3 (40% of white egg): water content was 2.59% (bb); fat content was 20.39% (bb); protein content was 9.11% (bk); brightness value was 64.95; texture value was 526 gf/mm 2; blooming capacity was 440%; hygroscopicity was 1.22%; oil absorption 18.85%; color sensory value was 4.6 (like to very like); taste sensory value was 3.88 (rather like to like); crispness sensory value was 4.24 (like to very like); the overall sensory value was 4.32 (the favorable to the most favorable).

Tempe Gembus Intervention Decreased Total Cholesterol And Triglyceride In Obese Women

Obesity increases cardiovascular risk through dyslipidaemia. High fibre diet reduces risk factors of dyslipidaemia by up to 50%. Tempe gembus is a solid fermented food made from tofu waste product with rhyzopus-oligosporus, that contained high fibre. The purpose of this study is to determine the effect of the administration processed tempe gembus on lipid profiles in obese women. This study was pre-post randomized control group design. Forty-four pre-menopausal women with obesity, dyslipidaemia took part in this study, divided into 2 groups, includes the intervention group and the control group. The Intervention group received 150 gram of processed tempe gembus for 28 days. Statistical analysis independent sample test or Mann Witney were used to analyse differences pre and post-lipid profile between intervention and control group. Consumption of 150 g/day of processed tempe gembus for 28 days reduced total cholesterol levels by 13.4 mg/dl (p = 0.012), HDL cholesterol by 2.8 mg/dl (p = 0.082), LDL cholesterol by 17.6 mg/dl (p = 0.52) and triglyceride levels of 5.3 mg/dl (p = 0.05), respectively. There was significant effect of processed tempe gembus consumption to reduce total cholesterol, and triglycerides. Unfortunately, there were no effect on LDL and HDL cholesterol.

Efek Pengaplikasian Sitokinin Dan Paclobutrazol Terhadap Ukuran Rimpang Tanaman Kunyit (Curcuma Domestica Val.)

Since COVID-19, the demand for turmeric has continued to increase both domestically and for export opportunities. This encourages the continued development of turmeric cultivation. The use of growth regulators can be done to regulate plant growth patterns. This study aims to determine the effect of the application of cytokinin and paclobutrazole on the size of the rhizomes of turmeric plants (Curcuma domestica Val.). This study used a Randomized Group Design (RAK) with 2 treatment factors, which are : Factor I Cytokinin (S) consists of 3 kinds, namely S1: 0 mg/l water, S2: 10 mg/l water and S3: 20 mg/l water while, Factor II: Paclobutrazole (K) consists of 4 kinds, namely: P1: 0 g/l water, P2: 0.50 g/l water, P3: 0.75 g/l water, P4: 1 g/l water. Based on the results of the experiment, it shows that the application of ZPT Cytokinin (BAP) and Paclobutrazole on turmeric plants gives a real interaction effect on the observation of vegetative growth of plant height, while on the harvest observation variable only gives a real interaction effect on the observation of plant biomass and not real on the number of tubers and tuber weight. The concentration and time of application of cytokinin (BAP) and paclobutrazole will affect the nature of stimulation and inhibition of growth, the application of BAP at a concentration of 10-20 mg / l water can increase growth but if combined with paclobutrazole above 0.75 g / l water will actually have an inhibiting effect.

Macam Varietas Tanaman Selada Perlakuan Media Organik

This research seeks to assess how organic and varietal planting media impact the growth and yield of lettuce plants (Lactuca sativa. L). The study was conducted using the Complete Group Random Design (CGRD) method with two treatment factors. The first factor involved three types of organic planting media: (M1) a blend of soil, manure, and burnt husks; (M2) a blend of soil, manure, and cocopeat; (M3) a blend of soil, manure, burnt husks, and cocopeat. The second factor comprised three varieties: (V1) Grand Rapid green lettuce, (V2) Red Rapid red lettuce, and (V3) Lollo Rossa red lettuce. The findings indicate that treatment (M3), consisting of soil, manure, burnt husks, and cocopeat, influences leaf width, plant weight, and dry stash weight. The best yield was observed in treatment (V1) with the Grand Rapid green lettuce variety showing significant effects across all parameters. The conclusions are as follows: 1. Organic growing media does not affect all observation parameters related to the growth and yield of lettuce plants. 2. Varietal differences have a noticeable impact on all observation parameters related to lettuce growth and yield. 3. The interaction between organic growing media and plant varieties significantly affects the observation parameters of plant height and plant weight, while it does not affect leaf width and dry stash weight.

The effects of sweet potato (Ipomoea batatas L.) extract and stevia extract on the physicochemical characteristics of effervescent granules

This study aims to determine the effects of the ratio of sweet potato extract and stevia on the physicochemical characteristics of effervescent granules. This study uses the Factorial Randomized Group Design with three repetitions. The treatment design carried out in this study consists of one factor, namely, the comparison of sweet potato extract consisting of six levels, namely, (37.5:7.5), (35:9), (32.5:11.5), (30:14), (27.5:16.5), and (25:19). Responses in this study include chemical responses, namely, water content, pH value test, total sugar content, and anthocyanin content. Physical responses include dissolving time, hygroscopicity, color intensity, and scanning electron microscope. The comparison of sweet potato extract with stevia results showed an effect on effervescent granule characteristics, namely, total sugar content, dissolving time test, hygroscopicity level, and color intensity. This includes a moisture content of 2.56%, pH value of 3.45, total sugar content of 1.61%, and anthocyanin content of 21.19 mg/g. The best physical response results are dissolving time of 39.96 s, hygroscopicity level of 0.33%, and color intensity L* 49.46, color a* 16.55, and color b* -4.21.

Respons Agronomi dan Fisiologi Genotipe Padi (Oryza sativa L.) pada Budi Daya Berkelanjutan

Efforts to maintain rice production in the future face many challenges, including the availability of increasingly narrow land, attacks by plant pest organisms, and declining land fertility due to improper cultivation systems. More than 70% of rice fields are in a disturbed ecological status due to cultivation technology that could be more environmentally friendly, which can interfere with the sustainability of rice self-sufficiency. An environmentally friendly rice cultivation system needs to be developed. This study aims to measure the agronomic and physiological responses of 3 rice genotypes in sustainable cultivation systems. The experiment used a Group Random Design on 3 rice genotypes (black, red, and white) in both conventional and sustainable cultivation systems. The research was carried out in the experimental garden of Polinela Organic Farm, Lampung State Polytechnic, from September 2020 to September 2021. The results showed that the plant, leaf greenery, number of tillers, number of panicles, grain weight per panicle, and biomass weight were not significantly different between conventional and sustainable cultivation. At the same time, the harvest and production indices per hectare were significantly different. The physiological responses to the photosynthesis and transpiration rates differed markedly in conventional and continuous cultivation. In contrast, the conductance of stomata did not differ significantly in both cultivation systems. The red rice genotype is the best, and it has a broader adaptation to conventional and sustainable cultivation systems. Keywords: agronomy, conventional sysem, physiology, rice genotype, sustainable system

Yield, milling quality, rice quality and preferences of superior and specific location rice varieties

Abstract Grain and rice characteristics are a description of the post-harvest quality of rice which is a consideration for farmers, traders, and consumers in choosing rice to be developed and consumed. This study aims to determine the yield, milling quality, rice quality and preference of 5 rice varieties in Indonesia, they were Inpari 32 HDB, Inpari 42 Agritan GSR, Inpari 45 Dirgahayu, Pamelen and Inpari IR Nutri Zinc. The study used a non-factorial Randomized Group Design, rice varieties as treatments, 5 replications. Rice was harvested using 2 methods, namely sickle (manual harvesting) and combine harvester. Determination of Milling Quality, rough rice was milled using a mini electric grain whitener LTJM-2008 then measured the percentage of hulled rice, unhulled rice, bran, and husk. Determination of rice quality, 25 grams of rice samples were sorted manually and measured the percentage of head rice, broken, groats and damaged then determined the grade of rice based on SNI 6128: 2015. The results showed that the rice yield manually and using a combine harvester was different from the potential yield. The milling quality and rice quality of each variety were significantly different (p=0.05%). Rice quality classification based on SNI 6128:2015 shows that Inpari 42 Agritan GSR and Inpari IR Nutri Zinc was included in Medium I, pamelen and Inpari 32 HDB were included in Medium II, and Inpari 45 Dirgahayu was under Medium III. Inpari 32 HDB was the rice variety with the highest preference.

Effect of refugia plant (Zinnia sp.) population on the presence of stem borer (Scirpophaga innotata Walker) and natural enemies in rice

Abstract. Olsiviana, Yassi A, Melina. 2024. Effect of refugia plant (Zinnia sp.) population on the presence of stem borer (Scirpophaga innotata Walker) and natural enemies in rice. Biodiversitas 25: 2652-2660. This study supports biological control by preserving natural enemies in rice cultivation through habitat modification. The research aimed to analyze the effects of different population levels of refugia (Zinnia sp.) on pest attack rates, the presence and diversity of natural enemies of rice stem borer (Scirpophaga innotata Walker 1863), and rice (Oryza sativa L.) production. The study was designed using a Randomized Group Design (RGD) with four population treatment levels: control (no Zinnia sp.), sparse population (32 polybags of Zinnia sp.), medium population (96 polybags of Zinnia sp.), and dense population (160 polybags of Zinnia sp.). The results showed that the dense population (160 polybags of Zinnia sp.) produced the lowest stem borer infestation rate in the eighth week of observation (1.67%). Pests in all treatments belonged to four orders: Lepidoptera, Hemiptera, Coleoptera, and Diptera, with Hemiptera being the most prevalent. Natural enemies of rice pests belonged to three orders: Coleoptera, Hymenoptera, and Orthoptera. The dense population treatment (160 polybags of Zinnia sp.) yielded the highest values for diversity index (H') at 2.1785, species richness index (R) at 2.1837, and evenness index (E) at 0.9915. This treatment also achieved the highest average rice production at 8.64 tons/ha. Meanwhile, the highest dominance value (C) of 0.2489 was found in the control treatment. Thus, this study supports the idea that increasing the population of Zinnia sp. as a refugia plant can increase the diversity of natural enemies, reduce rice stem borer infestation, and increase rice plant production.

Aplikasi Streptomyces sp. ASR 67 dan Pupuk Organik Cair dalam Mengendalikan Penyakit Layu Fusarium oxysporum dan Pemacu Pertumbuhan Tanaman Kentang (Solanum tuberosum L.) Varietas Granola

Demand for the Granola variety of potatoes continues to increase, but its productivity is not yet sufficient for market needs. This is due to the wilt disease attack on potatoes caused by the fungus Fusarium sp., thereby reducing potato productivity. The aim of this research was to determine the use of liquid culture of Streptomyces sp. ASR 67 and liquid organic fertilizer as an organic anti-fungal in controlling wilt disease caused by Fusarium oxysporum on potato plants (Solanum tuberosum L.). The research was carried out using a Randomized Group Design (RAK) method using five treatments. Each treatment was repeated five times and each repetition used one potato plant. Treatment was given by watering the potato growing medium for 30 days, which was done every three days. The results showed that administration of liquid culture of Streptomyces sp. ASR 67 10% combined with liquid organic fertilizer (1:9) was able to increase plant growth with the parameters of height, number of leaves, number of stems, weight of tubers significantly, while POC 2% and Streptomyces sp. ASR67 (1:1) is able to reduce the incidence of wilt disease best.

Pengaruh Pupuk ‘Mutiara’ dan Giberelin Terhadap Pertumbuhan dan Produksi Tanaman Semangka Partenocarpi

Watermelon production is influential in supporting the increase of income of the farm. Watermelon production tends to decline at present, so that efforts need to be carried out to optimize the growth and yield of watermelon plants. One of the important nutrients source for watermelon plants is ‘Mutiara’ fertilizer and the plant growth regulator (PGR) gibberellin. This study aimed to determine the right combination of ‘Mutiara’ fertilizer dosage and gibberellin concentration on the growth and yield of parthenocarpic watermelon plants. This research was conducted from September to December 2023 at Pattimura Mutiara Farm of Pattimura University, in Rumahtiga Village, Teluk Ambon Subdistrict, Ambon City. This research was an experimental study that used a Factorial Randomized Group Design (RAK) consisting of two treatment factors, i.e.: ‘Mutiara’ fertilizer with 4 levels and the dose of gibberellin with 4 levels, with replications, so that there was a total of 48 treatment combinations. The observed variables included plant height, number of leaves, number of female flowers formed, fresh fruit weight, fruit diameter, and number of seeds. The results showed that the interaction between the dose of ‘Mutiara’ fertilizer 6 g/plant and the concentration of gibberellin 6 mL/L was the best treatment combination and could increase plant length and number of leaves. The combination of 2 g of ‘Mutiara’ fertilizer and 2 mL/L gibberellin concentration was the best combination for fresh fruit weight

Pertumbuhan dan Hasil Kedelai Edamame (Glycine max (L) Merill) Terhadap Pemberian Kombinasi Legin dan Kompos di Media Tailing Pasir Pasca Tambang Timah

Post tin mining land in Bangka Belitung can still be utilized as agricultural land with the application of various soil amandment including the combination of compost and legin. The compost provide organic matter for the soil and legin provide rhizobium to maximize nitrogen fixation in the soil. Post tin mining land improved with compost and legin combination can be used potentially for the development of edamame soybean commodities which are widely known as adaptive plant on suboptimal land. The purpose of this study was to determine the type of compost, legin dose and the best combination of both compost and legin on the growth and the yield of edamame soybeans in sand tailing media. This research had been conducted in February-August 2023 at the Experimental and Research Station, Faculty of Agriculture, Fisheries and Marine, Universitas Bangka Belitung. This study used a field experimental method with a Factorial Randomized Group Design with the first factor was the dose of legin, consists of 3 treatment levels, namely: L0: No treatment (Control), L1: 10 g legin, L2: 12 g legin. The second factor was the type of compost consisting of 3 treatment levels, namely: P1: Cow dung compost, P2: rice husk compost, P3: Commercial compost. There were 9 treatment combinations that were replicated 4 times. Each experimental unit had 6 plants so that 216 plant populations were obtained and 144 plants were obtained as samples. The results showed that the dose of 12 g legin had the highest effect on edamame growth and 10 g legin treatment had the highest effect on edamame yield. Commercial compost treatment had the best effect to edamame growth, while rice husk compost had the best effect to edamame yield. The combination of rice husk compost and 10 g legin was the treatment combination that showed the best growth and yield of edamame soybean plants in sand tailing media of post tin mining. Keywords: Edamame, rice husk, compost, legin, sand tailings

Growth Response and Antibacterial Activity Test of Sintrong Plant (Crassocephalum crepidioides (Benth.) S. Moore) on different Growth Media and Shade

This study investigated the growth response and antibacterial activity of sintrong plants (Crassocephalum crepidioides (Benth.) S. Moore) on different growth media and shade to support organic functional food. The research was conducted at the Field Laboratory of the Faculty of Agriculture, Halu Oleo University using a two-factorial Randomized Group Design (RAK). Variations of planting media used were soil without fertilizer, a mixture of soil and cow manure in a ratio of 1:1, and a mixture of soil and cow manure in a ratio of 1:2. The variations of shade used were no shade, 25% paranet shade, and 50% paranet shade. Antibacterial activity was assessed using the Delusion method. Growth data and antibacterial activity were analyzed through Analysis of Variance (ANOVA) with SPSS application and continued with Duncan test at 95% confidence level (α = 0.05). The results showed that planting media and shade had a significant effect on the growth of sintrong plant height. The greatest growth occurred in a mixture of soil and manure 1:1 in 25% shade (P1N1), while the lowest growth occurred in a mixture of manure-soil 1:2 without shade (P2N0). Antibacterial activity testing showed a significant effect of various growing media and shade treatments. The highest antibacterial activity was found in sintrong plant extract grown without manure at 50% shade (P0N2), with an inhibition zone of 16.6 mm for Escherichia coli bacteria (gram negative) and 13.2 mm for Staphylococcus bacteria (gram positive). The lowest inhibition zone occurred in sintrong plants grown on 1:2 manure-soil mixture without shade (P2N0) with an inhibition zone of 8.0 mm for Staphylococcus aureus bacteria