This study evaluates the effect of using four different starter culture combinations in yogurt samples used in Yayık butter production. Two of the combinations were isolated strains (27 St/27 Lb) and (27 St/A Lb) used in Yayık butter samples of A and B. The other two were commercial cultures (CHI) and (Y080 F) used for samples C and D, respectively. The Yayık butter samples were analyzed in serum pH, titratable acidity, fat, acid degree value, peroxide value, free fatty acids, volatile compounds, and sensory properties in the 1st, 30th, and 60th days of storage. The serum pH differed significantly among the samples; lower in A and B than in C and D (P < 0.05). Storage time was significantly effective on the titratable acidity (°SH) of all Yayık butter samples (P < 0.01). The peroxide values in the samples, indicating oxidative stability, were lower than the threshold value of 2.0 meq O2/kg fat. Although not perceived sensorially, Yayık butter samples A and B had higher lipolytic activity resulting in higher free fatty acid (FFAs) accumulation, compared to C and D. Contrarily, only a very mild rancid flavor defect was pronounced for C on day 60. Among the thirty-five volatile compounds detected in the samples, the ketones (2-butanone and 2-heptanone) were predominant during the storage period. The highest amount of butyric acid was detected in A followed by D on all storage days. Sample A got the highest scores in all sensory attributes followed by sample B.
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