Ramphastos Toco Research Articles

Toco toucan documented as prey for the black‐and‐chestnut eagle in Argentina

Toco toucan documented as prey for the black‐and‐chestnut eagle in Argentina

Behavioural changes in aposematic Heliconius melpomene butterflies in response to their predatory bird calls

Prey-predator interactions have resulted in the evolution of many anti-predatory traits. One of them is the ability for prey to listen to predators and avoid them. Although prey anti-predatory behavioural responses to predator auditory cues are well described in a wide range of taxa, studies on whether butterflies change their behaviours in response to their predatory calls are lacking. Heliconius butterflies are unpalatable and form Müllerian mimicry rings as morphological defence strategies against their avian predators. Like many other butterflies in the Nymphalidae family, some Heliconius butterflies possess auditory organs, which are hypothesized to assist with predator detection. Here we test whether Heliconius melpomene change their behaviour in response to their predatory bird calls by observing the behaviour of male and female H. m. plessini exposed to calls of Heliconius avian predators: rufous-tailed jacamar, migratory Eastern kingbird, and resident tropical kingbird. We also exposed them to the calls of the toco toucan, a frugivorous bird as a control bird call, and an amplified greenhouse background noise as a noise control. We found that individuals changed their behaviour in response to jacamar calls only. Males increased their walking and fluttering behaviour, while females did not change their behaviour during the playback of the jacamar call. Intersexual behaviours like courtship, copulation, and abdomen lifting did not change in response to bird calls. Our findings suggest that despite having primary predatory defences like toxicity and being in a mimicry ring, H. m. plessini butterflies changed their behaviour in response to predator calls. Furthermore, this response was predator specific, as H. m. plesseni did not respond to either the Eastern kingbird or the tropical kingbird calls. This suggests that Heliconius butterflies may be able to differentiate predatory calls, and potentially the birds associated with those calls.

Radiologic and computed tomography evaluation of the beaks from three bird species.

This study aimed to evaluate the beaks of three species of birds using radiography and computed tomography (CT). The mean lengths of maxillary and mandibular rostra on radiographs were highest for toco toucan, followed by buff-necked ibis, and least for red-legged seriema birds. The height and width of maxillary and mandibular rostra measured on CT had mean values highest for toco toucans, followed by red-legged seriema, and least for buff-necked ibis. Except for the proximal region of the maxillary rostrum, the HU values were positive for other regions of the maxillary and mandibular rostra in the buff-necked ibis and red-legged seriema and negative in all for the toco toucan.

Radiographic and sonographic features, and histogram parameters of the liver and spleen in healthy Toco toucans (Ramphastos toco, Müller 1976).

This study aimed to evaluate radiographic and sonographic features, and histogram parameters based on grayscale ultrasound of the liver and spleen in healthy toco toucans. Fifteen adult toco toucans (Ramphastos toco), seven females and eight males, weighing approximately 650 g, were enrolled in the study. On the right lateral radiographic view, the liver was visualized in the midventral region of the coelomic cavity; ultrasonographically, the liver was located in the middle portion of the coelomic cavity in close relationship to the heart, and thoracic and abdominal air sacs. Two hepatic lobes were identified; the right lobe was larger than the left one. The spleen was visualized in 10 toco toucans on radiographs and only in eight toucans on ultrasound exams. The gallbladder was identified only on ultrasound. On the right lateral radiographic view, the spleen was visualized dorsal to the proventriculus and ventral to the lungs/air sacs as an oval shape. Ultrasonographically, the spleen was observed caudal to the liver, cranial to the proventriculus and craniodorsal to the ventriculus. There was no significant difference in spleen length for either radiographic or ultrasound measurements. The brightness intensity (BI) for mean graylevel (G) and standard deviation of gray levels (SG ) in the liver were 101.315 (± 16.170) and 12.453 (± 2.616), respectively. Mean G and SG levels in the spleen were 63.940 (± 18.321) and 7.494 (± 3.595), respectively. In conclusion, the sonographic features and histogram parameters indicated that the liver is more echogenic and heterogeneous than the spleen, which must be considered for diagnosing alterations in these organs.

Comparing different citizen science platforms for collecting urban ecological data from Toco toucan (Ramphastos toco) in Argentina

AbstractCitizen science projects are valuable tools since they provide substantial amounts of data on different animal species. Currently, there are a wide variety of platforms and objectives for diverse citizen science projects. Despite these advances, the approaches and contributions of citizen science platforms for collecting data on a particular species, and in a specific site or region, have been poorly explored. Thus, in this article, we compare the number of participants and the amount of data collected by three platforms on the Toco toucan (Ramphastos toco) in San Salvador de Jujuy city, located in northwestern Argentina. This particular bird species is very charismatic and frequents urban areas all year round; so that, it is interesting to know what contributions (e.g. participant numbers, ecological and distribution data) are being made about this species on citizen science platforms. Thus, we examined data reported by two global citizen science platforms, iNaturalist and eBird, and one local platform, Tucanes en mi Jujuy (TEMJ). We found that the local, focal TEMJ project had a greater number of participants (n = 193), greater presence records (n = 614) and a wide spatial distribution of data in the city. In the iNaturalist and eBird platforms, the number of participants was smaller (n = 9 and 36, respectively), with fewer presence records (n = 12 and 178, respectively), and even fewer reports of species‐specific ecological data (feeding, behaviour and age). This reflected clear differences between the three platforms, as TEMJ not only reported more presence data but also more detailed information on age and behaviour. Based on this research, we highlight the commitment of citizens to this particular project and the study of species. Therefore, we recommend the implementation of new local and focal citizen science platforms for more detailed ecological data on other charismatic and focal species.

Seasonal variation in the predation on avian resources by the Toco Toucan (Ramphastos toco) in Brazil, based on community science data

La familia Ramphastidae (Piciformes) es endémica al Neotrópico e incluye 36 especies. Aunque son consideradas principalmente aves frugívoras, también se alimentan de invertebrados y pequeños vertebrados. El objetivo de este estudio fue examinar el consumo de recursos aviares por parte del Tucán toco (Ramphastos toco), el Ramphastidae más común en el Brasil no amazónico. En abril de 2022 se realizó una búsqueda de registros fotográficos de esta especie en WikiAves, la mayor base de datos de ciencia ciudadana sobre aves brasileñas. Un total de 271 fotografías tenían evidencia de actividades de alimentación. La mayoría involucraba recursos no aviares (85%), como frutas, mientras que una porción menor se refería a recursos aviares (15%). Estos 42 registros que involucran recursos aviares fueron más numerosos en octubre-diciembre que, en otros períodos del año, demostrando el consumo de huevos (50%), polluelos (45%) y aves juveniles o adultas (5%). Estos registros de alimentación incluyeron especies de las familias Tyrannidae, Columbidae e Icteridae, teniendo al Bichofué (Pitangus sulphuratus) como la especie de presa más común. Por otro lado, los registros que involucraban recursos no aviares ocurrieron a lo largo del año. Como resultado, la proporción de fotografías con recursos aviares fue mayor en octubre-diciembre que en otros períodos. Esta frecuencia relativamente alta de recursos aviares en la dieta del tucán toco coincide con su período de anidación en Brasil. Con nuestro estudio reconfirmamos la utilidad de la ciencia participativa para mejorar nuestro conocimiento sobre la dieta y la ecología alimentaria de las aves neotropicales.

Energetic costs of bill heat exchange demonstrate contributions to thermoregulation at high temperatures in toco toucans (Ramphastos toco).

Body temperature regulation under changes in ambient temperature involves adjustments in heat production and heat exchange rates between the animal and the environment. One mechanism involves the modulation of the surface temperature of specific areas of the body through vasomotor adjustment. In homeotherms, this thermoregulatory adjustment is essential for the maintenance of body temperature over a moderate temperature range, known as the thermal neutral zone (TNZ). The bill of the toco toucan (Ramphastos toco) has been described as a highly efficient thermal window and hypothesized to assist in the thermal homeostasis of this bird. Herein, we directly evaluated the contribution of heat exchange through the bill of the toco toucan and role of the bill in the delimitation of the TNZ. To do this, we measured metabolic rate (MR), via oxygen consumption, over a range of ambient temperatures from 0 to 35°C. MR measurements were made in birds with the bill intact and with the bill insulated. The limits of the TNZ did not differ between treatments, ranging from 10.8 to 25.0°C. The MR differed among treatments only at elevated temperatures (30 and 35°C), reaching 0.92±0.11ml O2g-1h-1 (mean±s.d.) for the intact group and 1.13±0.13ml O2g-1h-1 for the insulated group. These results indicate that although heat dissipation through the bill does not contribute significantly to widening of the TNZ, it may well be critically important in assisting body temperature regulation at higher temperatures extending above the upper limit of the TNZ.

Toucans’ ostentatious beaks do not expand their thermoneutral zone

Some birds are just instantly recognisable. From elegant flamingos with graceful necks to toco toucans (Ramphastos toco) with their jet-black plumage and distinctive beaks, some birds just stand out from the dowdy crowd. And the toucan's outsized beak comes in handy for retrieving fruit and cracking nuts. But Glenn Tattersall from Brock University, Canada, explains that the toucan's outrageous bill also doubles as a radiator; he recalls how, in the 2000s, Augusto Abe (Sao Paulo State University, Brazil) put him on to the possibility that the birds may use their beaks to regulate their body temperature. However, warm-blooded creatures mainly prefer living at temperatures where they expend little energy to remain warm or keep cool, known as their thermoneutral zone. Denis Andrade, Jussara Chaves (both from Sao Paulo State University) and Tattersall wondered whether the toucans’ ostentatious beaks could expand the range of temperatures over which the birds live at relatively little cost to maintain their body temperature.Collecting six toucans, Chaves settled the birds in Andrade's lab at the Universidade Estadual Paulista, Brazil. Then each night, she placed individual birds into a temperature-controlled chamber – set at temperatures from 0 to 35°C, spanning the 10–25°C temperature range where they expend little energy to remain warm – and measured their oxygen consumption to find out how much energy they used to regulate their temperature when hot and chilly. Then, Chaves repeated the oxygen consumption measurements, but this time she prevented the birds from using their beaks like a radiator by placing a snuggly fitting felt jacket over each beak, which allowed the birds to open their beaks if they got warm and needed to pant, while preventing their beaks from absorbing or losing heat to regulate their temperature. If the beaks were allowing the birds to expand the range of temperatures over which they could live a relatively low-cost life, then the felt beak-jacket would alter the temperatures at which the birds began using more energy to keep warm or cool.But the beak-jacket didn't extend or reduce the temperature range over which the birds live comfortably. Their thermoneutral zone is not extended by their radiator beaks.The team also reviewed how the birds slept in cold conditions and realised that the chilliest birds with unjacketed beaks tucked their beaks beneath their wing feathers, in addition to shutting off the blood supply to the beak, to keep warm. However, as the temperatures rose above 30°C, the birds’ naked beaks came into their own. The toucans with felt jacketed beaks used 20% more energy than those without beak-jackets, probably because they were panting to keep cool. ‘Our estimate is that the beneficial consequence of being able to access the normal heat exchange through the bill at 35°C corresponds to ∼32% of basal metabolic rate’, Tattersall explains.So, the toucan's beak can work both ways. ‘It promotes heat dissipation quite efficiently, but under conditions of high solar radiation it may also be a window to undesired heat gain’, says Andrade. And the team suspects that the toucans’ ability to absorb heat through their impressive beaks as they go about their daily business could limit how long they spend foraging for food, forcing them to seek shade at the peak of the day to avoid overheating.

A Review of the Trade in Toucans (Ramphastidae): Levels of Trade in Species, Source and Sink Countries, Effects from Governance Actions and Conservation Concerns

Utilising wildlife as natural resources has a long history and wide appeal for many nations, while seeking international wildlife that is sustainably managed is the primary responsibility of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). However, data-driven trade reviews are required, utilising CITES data to increase our understanding of the trade and facilitate evidence-based conservation planning. This study presents the first trade review for Toucans utilising CITES import reported data. The total number of Toucans exported was over 22,000, which subsequently generated a retail ‘real price’ value of nearly US $72 million. The countries accounting for the majority of Toucan exports were Guyana (39%), Suriname (33%) and Nicaragua (14%), while the main importing country was The Netherlands (nearly 25%). Toucan species traded were Ramphastos vitellinus (accounting for 21.5%), Ramphastos toco (19%) and Ramphastos tucanus (17%), making the top three while trade was recorded in 10 species. However, successfully identifying economic values for 15 species highlights that trade exists within non-CITES listed Toucan species too. Therefore, the levels of trade in non-CITES-listed Toucan species need urgent attention, as do the non-detrimental findings that underpin the CITES quotas set for each species, given the species’ importance ecologically.

Lafora’s disease in a free-ranging toco toucan (Ramphastos toco) with neurologic disease

Lafora’s disease is a genetic disease associated to mutations in genes that encodes laforin and malin, which results in intracellular polyglucan storage. The present report describes a case of Lafora’s disease in a toco toucan with episodes of incoordination and myoclonus that resulted in traumatic lesions and fracture of the left hindlimb. The bird was euthanized and submitted to necropsy. Microscopically there were abundant PAS-positive and diastasis-resistant Lafora’s inclusion bodies in neurons in the cerebellum, supporting the diagnosis of Lafora’s disease.

Will Brazilian City Dwellers Actively Engage in Urban Conservation? A Case Study with the Charismatic Neotropical Blue-and-Yellow Macaw (Ara ararauna)

The Blue-and-yellow macaw (Ara ararauna) is a charismatic and easily recognized species. While they are classified as being of “least concern” in the International Union for Conservation of Nature (IUCN) Red List, their populations are declining. In Brazil, the accelerated destruction of one of its key habitats, the Cerrado biome, is a principal cause of their decreasing abundance. As with other species affected by loss of the Cerrado, active conservation measures are required. While usually rare in urban ecosystems, Ara ararauna occurs and breeds in small numbers in the city of Rondonópolis, a medium-sized city located in Mato Grosso, Central-West Region of Brazil. Blue-and-yellow macaw pairs nest in the tops of dead palm trees, but as the macaws are nest-site limited and suitable dead trees are scarce in urban areas, the installation of artificial nest sites in domestic backyards could help that urban population to thrive. To investigate whether local people would be willing to engage with conservation efforts and in particular to support macaw conservation, we surveyed the attitudes of Rondonópolis residents to measure: (1) citizens’ preferences among the bird species that occur in the city; (2) the average area of their yards, and (3) the willingness of residents to: (a) plant fruit trees to attract macaws to their yards and (b) to install artificial nests in their yards. Larger and more colorful birds (Ara ararauna, the Red-and-green macaw Ara chloropterus and Toco Toucan Ramphastos toco) were the bird species most valued across all socio-economic groups, suggesting that the charismatic species would be more likely to be supported by urbanites. Overall, people had good biodiversity knowledge, with respondents being able to identify half of our sample of local bird species, on average, and almost half had seen a nest site. The great majority were willing to plant fruit trees (78%) and provide nest sites (70%) for macaws. This willingness to engage was not affected by socioeconomic differences. These data indicate that a municipal macaw conservation program could be successful in Rondonópolis, and we argue that macaws could be a flagship species which would benefit wider efforts to engage Brazilian urbanites in active engagement with nature.

Outbreaks of Acute Necrotic Enteritis and Haemolytic Crisis and Renal Disease Associated with Clostridium perfringens in Captive Toucans (Ramphastos spp)

Four toco toucans (Ramphastos toco), one channel-billed toucan (Ramphastos vitellinus) and one white-throated toucan (Ramphastos tucanus) died in two disease outbreaks in the same aviary in 2011 and 2016. Post-mortem examination revealed diffuse necrotic enteritis (NE) as the cause of death of five of these six birds. Clostridium perfringens was identified by culture and real-time multiplex PCR for C. perfringens α-, β-, ε- and ι-toxin genes in ligated intestine of one toucan from each outbreak. At another aviary, two keel-billed toucans (Ramphastos sulfuratus) died peracutely from severe haemolytic crisis with haemoglobinaemic nephrosis and cholestasis and acute tubulointerstitial nephritis. Mild NE was present in these birds and C. perfringens was demonstrated in liver by bacterial culture and real-time multiplex PCR for C. perfringens α-, β-, ε- and ι-toxin genes. To the authors' knowledge, this is the first description of outbreaks of NE associated with C. perfringens in captive toucans. Although haemolytic crisis has been reported in humans with C. perfringens type A septicaemia and hepatic abscesses, this presentation appears not to have been described in C. perfringens infections in toucans or other avian species. The factors causing C. perfringens proliferation and disease in the toucans were not identified. PCR for C. perfringens NetB toxin and enterotoxin genes performed retrospectively on one of the C. perfringens isolates from the second outbreak and on paraffin-embedded tissues from one dead toucan from the first outbreak was negative. With the current C. perfringens toxin typing scheme, C. perfringens type A was identified in the first two outbreaks.

Breeding Aspects of the Toco Toucan and the Chestnut-eared Aracari (Aves, Ramphastidae) in the Brazilian Cerrado

Toucans are among the most conspicuous and famous birds in the Neotropics, but numerous aspects of their biology remain poorly known. The Toco Toucan (Ramphastos toco) and the Chestnut-eared Aracari (Pteroglossus castanotis) are commonly found in central Brazil, where studies on their breeding are scarce. The objective of this study was to examine aspects of their breeding in the Brazilian Cerrado. Searches for photographs with evidence of breeding activities were done in the WikiAves database. Citizens produced 126 records of copulations, nests, eggs and/or young. Records of the Toco Toucan (n = 92) tended to be concentrated in central and southeastern Cerrado, while those of the Chestnut-eared Aracari (n = 34) occurred mainly in its central and southwestern regions. Nests of the Toco Toucan were in hollows of palm and non-palm trees, termite nests and a pole. They were in native or human-modified habitats, and had 2–3 eggs or young in the early rainy period (OctoberDecember). On the other hand, nests of the Chestnut-eared Aracari were in hollows of non-palm trees in unknown habitats; regarding these records, a single nestling was found in the nest entrance in November, and clutch and brood sizes were unknown. Records of copulations, eggs and young in the nest indicate that the onset of breeding season of both species occurs from the late dry season to the early rainy season. Records of young indicate that the breeding seasons might reach the dry season in the Cerrado. Citizen science can improve our knowledge about toucans.

Ultrasonography of the gastrointestinal tract of toco toucans (Ramphastos toco)

Baseline data for wall thickness in healthy toucans can be used to diagnose disease in sick individuals. This study aimed to evaluate the use of ultrasonography for the examination of the gastrointestinal tract of healthy toco toucans (Ramphastos toco). Ten non-anaesthetized healthy adult toco toucans were evaluated. Food was withheld for 3h prior to the ultrasound examination. Longitudinal and transverse images of the gastrointestinal tract were obtained using ventromedian cranial and ventromedian caudal approaches to evaluate the morphology and stratification. The wall thickness of the sections was measured: proventriculus and ventriculus, duodenum, jejunum/ileum, and cloaca. Additionally, the thickness of the mucosal layer of the duodenum and jejunum/ileum was also measured. Subsequently, the ratios of the duodenal wall thickness to the duodenal mucosa layer and the total wall thickness of the jejunum/ileum to the jejunum/ileum layer mucosa were stablished. The wall thickness of the proventriculus was significantly higher than the wall thickness of ventriculus. The wall thickness of the duodenum was significantly higher than the wall thickness of jejunum/ileum. The mean ratios of the duodenal wall thickness to the duodenal mucosa layer and the wall thickness of the jejunum/ileum to the jejunum/ileum layer were 83.15% and 80.07%, respectively. In conclusion, it was possible to assess the stomach and intestines using ultrasound examination in healthy toco toucans, due to their medium body size and no interference by surrounding air sacs.

Rediscovery of Plasmodium (Huffia) huffi (Apicomplexa, Haemosporida): a lost lineage from toucans.

Literature data on toucans haemosporidians are scarce and all reports come from investigations in Brazil. Muniz et al. (Rev Bras Malariol 3: 339-356, Muniz et al., Rev Bras Malariol 3:339-356, 1951) and Muniz and Soares (Rev Bras Malar 611-617, Muniz J, Soares R de RL (1954) Nota sôbre um parasita do gênero Plasmodium encontrado no Ramphastos toco Müller, 1776, "Tucano-Açu", e diferente do Plasmodium huffi: Plasmodium pinottii n. sp. Rev Bras Malar 611 - 617.) described two Plasmodium species, P. huffi and P. pinottii, in Ramphastos toco. Later, Manwell and Sessler (J Protozol 18: 570-574, Manwell and Sessler, Malaria Parasites of Toucans J Protozol 18:570-574, 1971) established a new subspecies, P. nucleophilum toucani. In the last review on avian haemosporidians, Valkiūnas (Valkiūnas, Avian malaria parasites and other haemosporidia, CRC Press, New York, 2005) highlighted that P. huffi was insufficiently characterized, considering it a lost lineage. Also, the original description of P. huffi was considered insufficiently clear, due to a possible co-infection of the toucan hosts with a Novyella-like species. Here, we redescribed the species Plasmodium (Huffia) huffi based on morphological and molecular data, which were found in two toucan species, Ramphastos toco and Pteroglossus aracari from Brazil. Morphological features of the specimens are markedly the same as the original description. In R. toco, we observe two individuals infected, one infected only with P. huffi and one co-infected with P. huffi and the Novyella-like species, as observed in the original description. Also, we observe one R. toco infected only with the Novyella-like species, identified by morphological and molecular data as Plasmodium (Novyella) nucleophilum nucleophilum. In this way, it was possible to redescribe Plasmodium huffi in detail, without the doubt characters observed in the original description. Moreover, by applying species delimitation algorithms to a large Plasmodium phylogeny, we were able to identify new possible hosts for P. huffi and extend its geographic distribution to include North America.

CLINICAL OBSERVATIONS OF BEE ENVENOMATION IN TOUCANS

There are numerous reports on envenomation, even fatal, secondary to bee attacks in humans and other mammals. In birds, reports on those incidents are scarce and there are none regarding honeybee (Apis mellifera) stings in toucans. In the first case presented, an adult female red-breasted toucan (Ramphastos dicolorus) received at least five bee stings in the periophthalmic area. Within 5 h the bird was lethargic and dehydrated. The urates were yellowish. Three days later the bird showed a moderate anemia, but no changes in the leukocyte count, beyond an elevated heterophil: lymphocyte ratio. Blood chemistry showed hyperglycemia, hypoalbuminemia and elevated aspartate aminotransferase and creatine kinase. Alterations in electrolyte values were also noted. Fourteen days later bile acid elevation was observed. Hematocrit levels normalized after 2 wk. A second incident involved a breeding pair of toco toucans (Ramphastos toco). While the female toco toucan received 10 stings and showed mild clinical manifestations, the male toco toucan was more severely attacked, receiving 40 stings, and died overnight. Despite the relative gravity of the attack (in terms of number of stingers in relation to body weight) both surviving birds recovered in less than 2 wk. To the authors' knowledge, fatal bee envenomation in birds has been reported only in pigeons and macaws. The findings described in this report suggest that toucans are less sensitive to bee venom when compared with pigeons and may have higher tolerance to bee venom compared with mammals. Honeybee envenomation must be considered a potential threat when considering toucan husbandry in zoos and collections.

Nivel de lactato sanguíneo en tucanes (Ramphastos toco)

Blood lactate concentration is often used in critically ill patients to identify tissue hypoxia, and its values have been referred to as prognostic markers in several settings, with blood lactate in Toco toucan never being described before. Hyperlactatemia is related as a sign of systemic and local hypoperfusion and as a prognostic indicator, being associated with the death of sick patients. The objective of this work was to describe the serum lactate levels in Toco toucan (Ramphastos toco) aiming to start a line of research using this analysis as a biological prognostic marker in this species. 17 free-living Toco toucan (Ramphastos toco) were used, temporarily kept in captivity at the Laboratory of Teaching and Research of Wild Animals (LAPAS) of the Federal University of Uberlândia (UFU). All were captured by the Environmental Police or IBAMA and sent to LAPAS for treatment, rehabilitation and subsequent release. The collection was performed for clinical monitoring of animals and, in the case of lactate, authorized by Opinion No. 365-2016 of the Ethics, Bioethics and Animal Welfare Commission of the University of Vila Velha (CEUA-UVV). In Toco toucan (Ramphastos toco) blood lactate values are between 4.2 and 6.7 mMol/L; however, further studies are needed comparing these results with others obtained in animals with specific pathologies, to characterize its use as a prognostic indicator and also moment of release.

Radiography and computed tomography of the heart and lower respiratory tract in toco toucans (Ramphastos toco).

This study aimed to evaluate normal features of the heart and lower respiratory tract in toco toucans by means of radiography and helical computed tomography (CT) scanner. Fifteen healthy adult toco toucans (Ramphastos toco), 10 females and 5 males, average body mass of 650g were studied. CT examination as well as right lateral and ventrodorsal radiographic examinations of the coelomic cavity were performed under chemical restraint. Heart, lungs, air sacs, trachea and syrinx were analysed. The mean values of heart length, heart width and thoracic cavity in radiographs were, respectively, 23.76mm, 25.94mm and 48.87mm. In both X-rays and CT scans, the lung parenchyma had honeycomb-like pattern. The topographic areas of the anterior and posterior air sacs were visualized as dark and air-filled spaces in X-rays. On CT evaluation, the air sacs occupied a larger area in the coelomic cavity compared to X-ray. In the lateral radiographic view, the cervical part of the trachea was positioned more ventrally in the transition from cervical to thoracic regions showing a V-shaped appearance. In all CT planes was visible division of the trachea into the right and left main bronchi at the level of 3rd thoracic vertebra. The syrinx was difficult to visualize in X-rays, but on CT it was easily identified in axial slice. In conclusion, the normal features of toco toucan's heart and lower respiratory tract that were determined on X-rays and CT scans are useful to compare with sick toco toucans, as well as other bird species.

Characterization of Newcastle disease virus obtained from toco toucan.

Given that the current Newcastle disease virus (NDV) infection in wild birds poses the threat to poultry, surveillance of Newcastle disease in captive wild birds was carried out in Jilin, China in 2018. Here, an NDV strain obtained from toco toucan was firstly characterized. The results showed that the F gene of the NDV isolate Toucan/China/3/2018 is classified as genotype II in class II. Sequence analysis of the F0 cleavage site was 113RQGR/L117, which supports the result of the intracerebral pathogenicity index assay indicating classification of the isolate as low-pathogenicity. Experimental infection demonstrated that Toucan/China/3/2018 can effectively replicate and transmit among chickens. To our knowledge, this is the first report on genetically and pathogenically characterizing NDV strain isolated from toucan, which enriches the epidemiological information of NDV in wild birds.

Yeasts and filamentous fungi in psittacidae and birds of prey droppings in midwest region of Brazil: a potential hazard to human health.

Birds of prey and from Psittacidae family are host to fungal microbiota and play an important role in the epidemiology of zoonoses. Few studies in the literature have characterized mycelial and yeast fungi in the droppings of these birds and correlated the isolates with the zoonotic potential of the microorganisms. Droppings from 149 birds were evaluated and divided into two groups: captive: Rhea americana araneipes, Primolius maracana, Ara ararauna, Ara chloropterus, Anodorhynchus hyacinthinus, Amazona aestiva, Ara macao macao, Ramphastos toco, Sarcoramphus papa, Busarellus nigricollis, Bubo virginianus nacurutu, Buteogallus coronatus, Buteogallus urubitinga urubitinga, Spizaetus melanoleucus, Spizaetus ornatus ornatus, Buteo albonotatus, Geranoaetus albicaudatus albicaudatus, Rupornis magnirostris magnirostris and Harpia harpyja, and quarantined birds: Amazona aestiva and Eupsitulla aurea. The fungal isolates were identified according to macroscopic (gross colony appearance), micromorphological and biochemical characteristics. Among birds displayed in enclosures, Aspergillus niger (41.1%) and Candida kefyr (63.8%) were the fungi most frequently isolated in Harpia harpyja and Ramphastos toco, respectively. For quarantined birds, the following percentages were observed in Eupsittula aurea , (76.6%) C. krusei, (84.4%) C. kefyr and (15.2%) C. famata, while in Amazona aestiva, (76.2%) C. krusei was observed. These findings indicate potentially pathogenic species in the bird droppings assessed, which constitute a risk of exposure for keepers and individuals who visit the zoo. Birds of the Cerrado and Pantanal of Mato Grosso (Central Western region of Brazil) could act in the epidemiological chain of important zoonoses.