Over 300 ejaculates from about 100 rams were exmained at the U. S. Sheep Experiment Station and Western Sheep Breeding Laboratory during annual Fall testing of rams. The relations between counts of sperm made with a Spencer Brightline hemacytometer and turbidity readings made with a Klett-Sommerson photoelectric colorimeter were analyzed by regression and covariance methods. Diluting fluids had significantly different effects on opacity of the diluted semen. Chlorazene was the diluter of choice, despite its change upon prolonged standing, because it was the only diluter capable of dissolving clumps which were prevalent in rams' semen. A dilution of 1:200 gave the optimum range of readings in the colorimeter scale, lower effect of debris, and lower error of estimate. Scores were established for estimating the amount of visible debris in the samples. Highly significant improvement in colorimetric estimation of sperm concentration was effected by scoring the amount of debris. Non-visible elements of debris, measured as residual turbidity after centrifugation of diluted samples, contributed appreciably to the colorimeter readings. This residual turbidity could not be used as an estimate of total debris. The colorimeter readings of semen with the debris removed gave a regression line with the intercept point not significantly different from zero. Two brands of diluting pipettes showed no differences in variability when rinsed into the diluted sample. They did show significant differences in volume and ability to drain without rinsing. The technique adopted for use (chlorazene at: 1200 with scoring of debris) gave a standard error of estimate of 38.1 (107 sperm per ml.). The error due to workers and instruments was negligible. The standard deviations for the two instruments were similar, 21.4 and 19.7, for sampling and dilution, but the hemacytometer was slightly less accurate, 26.7, because of its higher variance due to the Poisson distributional error of the smaller volume measured. The error of estimate attributable to scoring of samples for amount of debris was 22.7, while the error for debris and heterogeneity which could not be scored was 34.1.