Evaluation of genotoxicity related to nitrated polycyclic aromatic hydrocarbons (NO2-PAHs) was investigated on sediment contaminated with road runoff by using test system named umu-test which has highly sensitive potential with nitroarenes. The trace of genotoxicity on NO2-PAHs could be detected in the effluent from a sewage treatment plant where domestic sewage and rain water in urban area were introduced. And also, we obtained nitrated genotoxicity in the river water where the effluent was discharged. A source of the genotoxicity related nitro-derivatives would originate the sediment contaminated with road runoff, because mobile exhaust gas include many toxicants with nitro derivatives of PAHs. Road runoff water, especially, extracts of particulate matters showed strong genotoxicity on NO2-PAHs. Genotoxic potency on nitrated hydrocarbons could be obtained in the extract of particulate parts of water of a treatment plant of exhaust gas of motor cars with gasoline or diesel engines. It is possible to detect the trace of NO2-PAHs by this test system easily. The source and the potency for unit weight of each sample can be evaluated.
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