A 21-days experimental trial was conducted to examine the influence of feeding frequency on body composition of rainbow trout fingerlings. A number of 295 trout fingerlings (with mean initial weight ±SD of 5.53±0.25g), from Cislau fish farm, Buzau city, were equally stocked in four rearing units. The two experimental variants ( feeding frequency 2 meals/day -F1 and 4 meals/day -F2) were each applied for two tanks. At the end of the experiment, fish meat biochemical composition was significantly influenced (p<0.05) by feeding fr equency. Keywords: rainbow trout, meat biochemical composition, aquaculture recirculating systems Introduction. Optimum feeding frequency, for maximum fish growth may vary according to fish species, size and culture conditi ons, including feed quality, amount of feed administrated and water temperature values throughout the experimental trial (Lee et al., 2000). One of the factors that lead to choose an ap propriate feeding frequency consists in determining the time between meals, because feed consumption is closely related to stomach capacity, gastric emptying rate and digestion (Bret t, 1971; Riche et al, 2004). A feeding frequency between 1-3 meals/day is reported to be p roper for rainbow trout. Aims and Objectives. Knowing the fact that biochemical composition of meat is influenced by several factors such as fish size, ag e, environmental and feeding conditions, the present study was conducted to determine the influe nce of feeding frequency on growth and body composition of rainbow trout fingerlings, in c onditions of using different feeding frequencies. Materials and Methods. The experiment was carried out between October 25 and November 16, 2010 at the pilot recirculating system of Aquaculture, Environmental Science and Cadastre Department from Dunarea de Jos University of Galati. The experimental system consisted of 4 four growing units with a tot al volume of 0.336 m 3 (0.35◊0.80◊0.120m) each and water quality maintenance module, represented by water mechanical and biological filtration units, water sterilization unit (represe nted by a UV lamp) and water oxygenation unit and 295 trout fingerlings. The two treatments (feed ing frequency of 2 meals/day -F1 and 4 meals/day -F2) were each applied for two tanks. On the first six days of experiment, fish were fed with a ratio of 3%/BW/day and then, the ratio i ncreased up to 5% on the eighth day; after that the ratio remained constant till the end of th e trial. The rainbow trout meat biochemical determinations were performed on muscle tissue samples and later, from the mixture, homogeneous samples were taken. Proteins were determined with Gerhardt type equipment by using Kjeldahl method, fats were determined by Soxhlet solvent extraction method (petroleum ether) with Raypa extraction equipment, dry matter was determined by heating at temperature of 105 ±2oC using Sterilizer Esac and a sh was evaluated by calcification at temperatures of 550±20°C in a Nabertherm furnace. Results and Discussions. The results regarding the biochemical composition of rainbow trout meat, fed under different feeding fre quencies conditions are presented in Fig. 1 . After statistical analysis were made, significant d ifferences (p<0.05) were registered for all