Raft System Research Articles

Biostimulatory Action of a Plant-Derived Protein Hydrolysate on Morphological Traits, Photosynthetic Parameters, and Mineral Composition of Two Basil Cultivars Grown Hydroponically under Variable Electrical Conductivity

Hydroponics is a viable alternative to open field cultivation for year-round vegetable production in urban areas. However, the total dependence on external chemical inputs (fertilizers) makes these systems often less environmentally sustainable. In this perspective, the use of biostimulants could represent a valuable and eco-friendly tool to limit the excessive use of fertilizers without a negative impact on the yield. To this end, our work aimed to evaluate the productive and physiological response of two cultivars of ‘Genovese’ basil (Eleonora and Italiano Classico) for the industrial production of “pesto” grown for 22 days in two nutrient solutions with different electrical conductivity (1 and 2 dS m−1) and the application of two doses of protein hydrolysates (0.15- and 0.30-mL L−1 of Trainer® in the nutrient solution). The mineral profile was evaluated by ion chromatography coupled with a conductivity detector, while pigments were evaluated by UV-Vis spectrophotometry. Generally, the nutrient solution concentration did not significantly affect the fresh yield of the two cultivars tested. On the contrary, the use of the maximum dose of biostimulant (BT2 = 0.30 mL L−1 of nutrient solution) increased fresh yield, leaf area, and ACO2 by 20.7, 27.5, and 17.6%, respectively, compared with the control. Using the lowest dose of biostimulant (BT1 = 0.15 mL L−1 of the nutrient solution) reduced nitrate by 6.6% compared with the control. The results obtained showed that basil cultivation in a floating raft system combined with biostimulant in the nutrient solution could be an excellent solution to improve productivity, reduce nitrate, and cut fertilizer costs.

Different effects of oxysterols on a model lipid raft – Langmuir monolayer study complemented with theoretical calculations

Three oxysterols (7β-hydroxycholesterol; 7β-OH, 7-ketocholesterol; 7-K and 25-hydroxycholesterol, 25-OH) differing in the site of oxidation (ring system versus chain) and kind of polar group (hydroxyl versus carbonyl) were studied in lipid raft environment using the Langmuir monolayer technique complemented with theoretical calculations. Experiments were performed for the unmodified raft system, composed of sphingomyelin (SM) and cholesterol (Chol), and in the next step the raft was modified by the incorporation of oxysterol in different proportions. In the examined three-component system (Chol:SM:oxysterol), apart from interactions between the lipid raft components, the affinity of Chol to its oxidized derivatives also plays an important role. 25-OH was found to enhance interactions between SM and Chol and thus stabilize the raft, contrary to 7β-OH and 7-K, which exerted the fluidizing effect as well as the destabilization of the raft. Different action of oxysterols on model raft was observed. 7β-OH and 7-K, which are highly potent inducers of cell death caused raft destabilization, while 25-OH, which is the least toxic of the investigated oxysterols, was found to stabilize the raft.

Gastric ulcer healing by chebulinic acid solid dispersion-loaded gastroretentive raft systems: preclinical evidence.

Background: Chebulinic acid (CA), a component in Terminalia chebula, exhibits antiulcer activity, but has poor aqueous solubility. Raft-forming systems incorporating solid dispersions (SDs) of CA, were developed to overcome its poor biopharmaceutical properties and to prolong thegastric residence time for maximum activity. Methods: SDs were formulated by a solvent evaporation method using Eudragit EPO. Raft formulations consisted of sodium alginate as apolymer. Results: Release of CA in thedissolution mediumwas 40%, whereas SDs showed 95.45% release. TheCA raft system (20mg/kg) showed curative efficacy in analcohol-induced gastric ulcer modeland increased protectionwhen compared with omeprazole (10mg/kg)and CA suspension (20mg/kg). Conclusion: These studies demonstrated SD raft systems to be a promising approach for antiulcer therapy by CA.

Experimental Investigation of piled raft foundation on Cohesionless Soil

The combination of piles and raft foundation is known as piled raft foundation. Piled raft foundations have proven to be more cost-effective and capable of meeting safe bearing capacity and serviceability norms in the case of high-rise buildings on cohesionless soil. The behavior of a stacked raft foundation is influenced by the piles, raft, and soil. The stacked raft system’s bearing capacity is improved and settlement is minimized when the ground beneath the raft foundation bears the burden of supporting the applied loads. The piled raft foundation minimizes total settlement and improves bearing capacity more than the raft foundation. When isolated footings cover more than 70% of the building area under a superstructure, raft foundations are used, and the present study focuses on the vertical load bearing capability of piled raft foundation systems on cohesionless soil for concentric loading. The use of strategically positioned piles increases the load capacity of the raft while reducing differential settlement. The present study sheds some light on the use of piles as raft foundation settlement reducers, as well as the behavior of a piled raft in sand. A series of small-scale model experiments were carried out. The present investigation studies by varying pile length and alignment on the ultimate load of piled raft foundation. The results indicate that for a 10mm raft thickness, installing 4 piles, 6 piles, and 9 piles by varying L/D ratios of 5,10,15,20 carries significant load. In this present work for a 50mm length of pile, and the value of load improvement ratio increases by 36 percent, 60 percent, and 68 percent, respectively, when compared to plain raft.

Investigation on Isolation Improvement Performance of Floating Raft System with Particle Dampers

Investigation on Isolation Improvement Performance of Floating Raft System with Particle Dampers

Analysis and Design of Piled Raft Foundation Taking into Account Interaction Factors

In a combined piled raft foundation (CPRF) both raft and piles take their share of the total load applied. However, in practice, the contribution of a raft in taking load is usually ignored and the load is assumed to be supported on piles. This way of CPRF becomes excessively conservative and uneconomical. To economize the design, relative load sharing of raft and piles in CPRF has to be found. In this connection, different simplified methods have been developed, each one with some limitations. In this study, three simplified methods have been applied to two cases of pile‐raft systems. The methods include Randolph, Poulos‐Davis‐Randolph, and modified Poulos‐Davis‐Randolph. The first case is a hypothetical case consisting of a 12 m × 12 m raft supported on a square group of nine piles. The second case study is an actual eight‐story building to be constructed in Peshawar, Pakistan. The building is supported on a pile‐raft system, with raft resting on very soft clay underlain by dense sand. The two case studies are also modelled in the finite element program PLAXIS 3D for comparison. The results of all the simplified methods are comparable with PLAXIS 3D. However, the Randolph method is much closer to PLAXIS 3D for the two cases studied. Furthermore, it is also shown that piles in a piled raft system can be used as “stress reducers” as well as a “settlement reducers.” Additionally, the effect of interaction factors is also evaluated with the s/d ratio as well as with varying soil stiffness. It was concluded that ignoring these factors leads to a very unsafe design of the pile‐raft system.

The Impact of LED Light Spectrum on the Growth, Morphological Traits, and Nutritional Status of ‘Elizium’ Romaine Lettuce Grown in an Indoor Controlled Environment

The study examined the influence of light quality on the growth and nutritional status of romaine lettuce grown in deep water culture with a floating raft system using two different nutrient solutions. Four spectra of LED light were used with different ratios of R, G, and B lights (80:10:10, 70:10:20, 60:10:30, and 70:18:12). Two nutrient solutions with a low (A) and moderately high (B) nutrient content were used. Regardless of the nutrient solution, the RGB 70:18:12 light promoted the production of leaf biomass as well as inhibited the accumulation of K and Mg in the leaves. Moreover, those plants were characterized by a low Nitrogen Balance Index (NBI) and a high flavonol index. In the last week of cultivation, there was a strong decrease in K, P, and nitrates in the nutrient solution, and an increase in Ca. In the final stage of growth, symptoms of withering of the tips of young leaves (tipburn) were observed on the plants. The most damage was observed on the plants growing under 70:10:20, 70:18:12, and with the higher concentration of minerals in the solution (B).

Behavior of Piled Raft Foundation in Soft Clay Layer with Geo-Foam Application

Piled raft system proves to be more effective on such problematic soft soil conditions. It takes the high vertical load and used to bring the settlement, differential settlement and tilting of structure within the permissible limit. Piled raft system proves to be cost effective than the conventional pile foundation system. Piled raft foundation accounts for complex soil-structure interaction, which needs interaction between structural engineer and geotechnical engineer. Geo-foam application is introduced below the footing to enhance floating resistance. The performance behavior of piled raft foundation in clayey soil and layered soil is carried out using laboratory experimental and then verified by 2D PLAXIS. There is a significant relationship between the decreases in settlement of the piled raft due to hydrostatic uplift.

Kinerja Produksi Ikan Lele (Clarias gariepinus sp) dengan Ukuran Tebar Berbeda Pada Sistem Akuaponik

Catfish (Clarias gariepinus Burchell, 1822) is a type of freshwater fish that is prospectively cultivated. This study aims to analyze the production performance and economic analysis of catfish on aquaponics technology with floating raft systems. This research was conducted descriptively with two treatments and four replications. The treatments included fish sizes, which were 6-9 cm (A) and 3-4 cm (B). The fish used were fish with an initial weight of 7.13±1.30 g and 4.11±0.43 g, the plant used was water spinach (Ipomoea reptans poir) with an initial height of 8-10 cm. The results showed that there was a trend of increasing fish weight during the study from 7.13±1.30 g and 4.11±0.43 g to 110.02±6.09 g and 86.44±5.00 g at the end of the study. . The survival rate value during the study was 100% for all treatments, the feed conversion value ranged from 1.48-1.59. The highest specific weight growth rate was 1.60%/day. Water spinach production during catfish rearing can be done for 3 harvests. The analysis of the aquaponics business of the floating raft system is very efficient and profitable. The value of R/C ratio > 1 in all treatments means that the cultivation business is said to be feasible. Payback period of 0.57 years (A) and 0.19 years (B), the fastest return on investment is in treatment B. Aquaponics floating raft system with catfish stocking size 3-4 cm for 60 days of maintenance period provides the best production performance with SR value was 60.78%, specific weight growth rate was 0.051%/day, feed conversion was 1.18 and the best kale production was 75 kg. Economically, this research is feasible to do.

Sargassum sp. Growth Cultivated with Different Seed Weights on Floating Raft System in Ekas Bay East Lombok

Sargassum sp. is seaweed from the group of brown algae belonging to the Phaecophyceae class that can be found in Indonesian. Sargassum sp. contains polysaccharides in the form of alginate which can be used as raw materials and additives in the food, drug, and cosmetic industries. The aim of this study was to analyze the growth of Sargassum sp. which are cultivated with different seed weights on the floating rafts. The research was conducted from November 2020 to March 2021 in Ekas Bay, East Lombok, West Nusa Tenggara. The research method used is an experimental method using a completely randomized design (CRD) consisting of five treatments with four replications. The treatments were differences in the weight of the initial seeds used, namely treatment A (25 g), B (50 g), C (100 g), D (150 g), and E (200 g). Sargassum sp. Cultivation was carried out for a month. The results were the highest absolute weight was in treatment E with 550 g and the lowest occurred in A with 262.5 g. The highest specific growth was in treatment A with 8.13%/day and the lowest was in E with 4.4%/day. This study concluded that different seedling weights had a significant effect on the growth of Sargassum sp. cultivated using the floating raft method

Dewatering Effect on Piles Behaviour in Piled Raft System Due to Use PLAXIS-3D

The study has aimed to investigate the piles behaviour in the piled raft system through the loading process with the nearby line drain influence utilized as a dewatering operation through the two soil layers below the piles group. The pile behaviour has included axial force, bending moment, lateral displacement, and vertical displacement at different piles locations within the group, the corner, the edge and the centre. The three-dimensional finite element PLAXIS 3D has been used to imitate the trouble. The soil has been supposed to be consisting of two layers deposit, the upper layer has been the clayey layer obeying the soft soil criterion, and the other layer has been the sandy layer obeying the Mohr-Coulomb failure principle. The results have indicated a significant influence of line drain on the group's corner and edge piles, while the line drain's insignificant effect at other piles has been observed. Furthermore, after dewatering, the axial forces and the bending moment have been highly increased for external piles, while a contrary behaviour for centre piles has been noticed. All examined piles have suffered lateral and vertical displacement after dewatering.

Drag reduction characteristics of the skin made of micro floating raft arrays based on immersed boundary method

To improve the drag reduction performance of underwater vehicles, a skin composed of micro floating raft arrays and a bionic compliant wall is proposed. The micro floating raft is a miniaturization of the floating raft system which is widely used in vibration and noise reduction. The drag reduction characteristics of the skin with various structural parameters are simulated with the IB2d software based on the immersed boundary method. A considerable reduction (up to 15%) in the viscous force is observed for the skin with a certain range of l*, i.e., the spacing between the micro floating raft arrays, which is resulted from the decrease of the velocity near the wall and the increase of the boundary layer thickness. Meanwhile, the drag reduction performance of the skin is also improved by increasing the porosity coefficient α* of the compliant wall, as well as the stiffness ratio γ* and the intermediate mass m* of the micro floating raft, within limits. This study provides a new idea for designing the coating of underwater vehicles and reveals the corresponding drag reduction mechanism to a certain extent.

Protein Hydrolysate Combined with Hydroponics Divergently Modifies Growth and Shuffles Pigments and Free Amino Acids of Carrot and Dill Microgreens

Microgreens are the new sophisticated commodity in horticulture that boost the human diet with bioactive metabolites and garnish it with colors and tastes. Microgreens thrive well when cultivated in soilless systems, of which closed-loop soilless systems combined with biostimulant application can provide a sustainable, innovative method of growing microgreens. Daucus carota L. and Anethum graveolens L. microgreens were grown in greenhouse conditions implementing a floating raft system combined with a protein hydrolysate of leguminous origin as root application (0.3 mL L−1 nutrient solution). Growth, colorimetric parameters, macronutrients, chlorophylls, carotenoids, carbohydrates, free amino acids, and soluble proteins were assessed. The use of a protein hydrolysate in the nutrient solution engendered an increase in anthocyanins (+461.7%) and total phenols (+12.4%) in carrot, while in dill, the fresh yield (+13.5%) and ascorbic acid (+17.2%) increased. In both species, soluble proteins and total free amino acids increased by 20.6% and 18.5%, respectively. The floating raft system proved to be promising for microgreens and can ease the application of biostimulants through root application. Future research should also investigate the yield and nutritional parameter responses of other species of microgreens with the aim of large-scale sustainable production.

The behaviour of piled raft foundation in loose sandy soil

The conducts of pile raft basis depend on piles, raft, as well as soil. When designing a pile group, it is expected that only the piles carry out the applied loading. No contribution is given to soils underneath the raft because of the complicated interacting amid the piles and their cap with soils. When the soil under the raft foundation shares carrying the applied loads, this interaction will increment the piled raft system’s bearing capability as well as decrease the settlements. To comprehend the piled raft system’s complicated conduct, testing for twenty-seven experimental models was done within loose sands with a load applied to the piled raft foundation via a compressing jack then the measurement was done using a loading cell. Wide parametrical examinations had been executed with the variables number of piles, pile length, raft dimension ratio. The soil under the raft contributed a certain percentage in carrying the applied weights in the piled raft foundation. Similarly, there is an increase in the loading taken via piles to the overall load executed on pile raft with the growth of the number of piles in the series, and there is a decrease in the share of the soil under the raft for the applied loads when there is increasing within the piles’ amounts. The increment within the piles’ lengths causes an increase in the soil’s share underneath the raft, and the maximum settlement of the piled raft system does not exceed 5% of the raft width in all cases.

Nutrient Solution Deprivation as a Tool to Improve Hydroponics Sustainability: Yield, Physiological, and Qualitative Response of Lettuce

Hydroponics growing systems often contain excessive nutrients (especially nitrates), which could lead to a quality loss in ready-to-eat leafy vegetables and posing a health risk to consumers, if managed inadequately. A floating raft system was adopted to assay the production and quality performance of lettuce (Lactuca sativa L. cv ‘Maravilla De Verano Canasta’) deprived of the nutrient solution by replacement with only water, three and six days before harvest. Yield and quality parameters, mineral composition, pigments, organic acids, amino acids profile, soluble proteins, and carbohydrate content were determined. Nutrient solution deprivation six days before harvest resulted in a significant reduction in leaf nitrate (−53.3%) concomitant with 13.8% of yield loss, while plants deprived of nutrient solution three days before harvest increased total phenols content (32.5%) and total ascorbic acid (102.1%), antioxidant activity (82.7%), anthocyanins (7.9%), sucrose (38.9%), starch (19.5%), and γ-aminobutyric acid (GABA; 28.2%), with a yield reduction of 4.7%, compared to the control. Our results suggest that nutrient solution deprivation three days before harvest is a successful strategy to reduce nitrate content and increase the nutritional quality of lettuce grown in floating raft systems with negligible impact on yield. These promising results warrant further investigation of the potential effect of nutrient solution deprivation on the quality attributes of other leafy vegetables cultivated in floating raft systems and in a “cascade” growing system.

Growth of Basil (Ocimum basilicum) in Aeroponics, DRF, and Raft Systems with Effluents of African Catfish (Clarias gariepinus) in Decoupled Aquaponics (s.s.)

Basil (Ocimum basilicum) was cultivated in three hydroponic subsystems (i) a modified commercial aeroponics, (ii) a dynamic root floating (DRF) system, and (iii) a floating raft system in a decoupled aquaponic system in Northern Germany, Mecklenburg–Western Pomerania. For plant nutrition, aquaculture process water from intensive rearing of African catfish (Clarias gariepinus) was used without fertilizer. After 39 days, 16 plant growth parameters were compared, with aeroponics performing significantly better in 11 parameters compared with the DRF, and better compared with the raft in 13 parameters. The economically important leaf wet and dry weight was over 40% higher in aeroponics (28.53 ± 8.74 g; 4.26 ± 1.23 g), but similar in the DRF (20.19 ± 6.57 g; 2.83 ± 0.90 g) and raft (20.35 ± 7.14 g; 2.84 ± 1.04 g). The roots in the DRF grew shorter and thicker; however, this resulted in a higher root dry weight in aeroponics (1.08 ± 0.38 g) compared with the DRF (0.82 ± 0.36 g) and raft (0.67 ± 0.27 g). With optimal fertilizer and system improvement, aquaponic aeroponics (s.s.) could become a productive and sustainable large-scale food production system in the future. Due to its simple construction, the raft is ideal for domestic or semi-commercial use and can be used in areas where water is neither scarce nor expensive. The DRF system is particularly suitable for basil cultivation under hot tropical conditions.

Foliar and Root Applications of Vegetal-Derived Protein Hydrolysates Differentially Enhance the Yield and Qualitative Attributes of Two Lettuce Cultivars Grown in Floating System

Lettuce (Lactuca sativa L.) is a leafy vegetable cultivated widely for its fast and year-round production and its beneficial phytochemical content, which may be boosted further by plant biostimulants that are considered eco-sustainable means for enhancing horticultural crop production. A greenhouse experiment was carried out to evaluate the yield and qualitative parameters of two differently pigmented lettuce cultivars grown in a floating raft system either untreated or treated (leaf, root or leaf/root application) with vegetal protein hydrolysates (PHs). For foliar application (F), lettuce plants were sprayed at a dose of 3 mL L−1, whereas for root application, 0.15 (T1) or 0.3 (T2) mL L−1 was applied to the nutrient solution alone or in combination with foliar spray (T1 + F and T2 + F) with the same foliar concentration. Bio-morphometric and production data were collected after harvest. Physiological and plant nutrition assays included leaf gas exchange, leaf fluorescence, SPAD index, mineral content, carotenoids, total phenols, total ascorbic acid content and antioxidant activities. Cultivar-specific reactions to biostimulant application were noted: whilst the green pigmented cultivar thrived under nutrient solution applications and recorded higher yield by 82.7% (T1) or (T1 + F) and 71.7% (T2), the red cultivar thrived under combined treatments, yielding 55.4% (T2 + F) higher than control and providing the most concentrated phytochemical content. These latter treatments also engendered the highest SPAD index, Fv/Fm ratio, CO2 assimilation, stomatal conductance and transpiration. In addition, the T2 + F treatment boosted ‘Canasta’ hydrophilic antioxidant activity (21.9%) and total ascorbic acid (5.6-fold). Nutrient solution treatments alone proved advantageous when compared to foliar treatments, while mixed treatments proved genotype-specific. New research on genotype specificity of biostimulant effects is warranted for future use, in order to rationalize biostimulant application modes and dosages.

Regulated Salinity Eustress in a Floating Hydroponic Module of Sequentially Harvested Lettuce Modulates Phytochemical Constitution, Plant Resilience, and Post-Harvest Nutraceutical Quality

A mild salinity stress (eustress) may modulate the induction of the plant defense system in horticultural crops and the synthesis of phytochemical components able to enhance plant resilience, post-harvest performance, and the nutraceutical quality of produce. However, the choice of the correct eustress type and dose to induce the synthesis of these protective phytochemicals is pivotal to avoid potential interference with plant growth and productivity. In order to study how green and red lettuce (Lactuca sativa L.) plants equilibrate the nutritional and nutraceutical components of quality with yield components, we applied iso-osmotic concentrations of three different salts (20 mM NaCl, 20 mM KCl, and 13.3 mM CaCl2, with a final total ionic concentration of 40 mM) in combination with two successive harvests in a floating raft system. The biometric parameters, mineral composition, bioactive compounds, and antioxidant activity of both cultivars were analyzed. The green cultivar had a superior response concerning biometric traits and productivity compared to the red one during the first cut but lower phytochemical content (e.g., ascorbic acid). The effect of cut order, independently of cultivar and salinity treatments, demonstrated that at the first harvest plants could redirect metabolism by increasing the lipophilic antioxidant content (LAA) at the expense of plant yield, therefore increasing plant resilience and post-harvest nutraceutical quality; whereas, at the second harvest, plants reverted principally to tissue expansion. The treatments with iso-osmotic salt concentrations did not affect K and Mg ion contents but further increased LAA and resulted only in a moderate decrease of fresh yield. The lettuce nitrate content was reduced during the second cut only when lettuce plants were treated with NaCl and especially CaCl2.

A Parametric Study of Piled Raft Foundation in Clay Subjected to Concentrated Loading

The use of piled raft foundation in building and infrastructure constructions is increasingly popular because of its effectiveness in reducing overall and differential settlements. Parameters influencing the performance of the piled raft foundation need to be comprehended in order to optimize the design of the piled raft system. Most of the current available literature focused on the piled raft foundation subjected to a uniform distributed load in sandy material. This parametric study aims to provide insights into the performance of the piled raft foundations subjected to concentrated loading in clay. A series of 2D finite element analyses were performed to investigate the influencing parameters affecting the load distribution and settlement behaviour of the piled raft. The results suggested that increases in both pile length and raft thickness, as well as a decrease in pile spacing would reduce the differential settlement of the piled raft. Comparatively, raft thickness was the most significant controlling parameter affecting the differential settlement. The study also revealed the importance of placing the pile nearer to the location of concentrated load as it would yield a more uniform load distribution, and hence a lower differential settlement.

Morpho-Physiological Responses and Secondary Metabolites Modulation by Preharvest Factors of Three Hydroponically Grown Genovese Basil Cultivars.

Sweet basil (Ocimum basilicum L.) is an economically important leafy vegetable especially in Mediterranean countries. In Italian gastronomy, the large elliptical leaves of the Genovese type are mostly used for the well-known pesto sauce, and almost all (>90%) professional production is for the food industry. The growing demand for fresh leaves with standardized technological and sensory characteristics has prompted basil producers to adopt advanced cultivation methods such as the floating raft system (FRS). The aim of this study was to evaluate the productive, qualitative, and physiological performance of three Genovese basil cultivars (“Aroma 2,” “Eleonora,” and “Italiano Classico”) in two successive harvests and at two densities (159 and 317 plants m–2). Caffeic, chicoric, rosmarinic, and ferulic acid were determined through the high-performance liquid chromatography (HPLC) system, whereas the extraction and quantification of the volatile organic compounds (VOCs) were performed by solid-phase microextraction (SPME) and gas chromatography coupled to a mass spectrometer (GC/MS). “Aroma 2” showed the highest fresh yield and photosynthetic rate together with the lowest nitrate content. For all the tested cultivars, the higher density, while reducing the number of leaves per plant, resulted in higher fresh and dry production per unit area, without altering the aroma profile. Successive harvests resulted in a significant increase in both the yield (37.5%) and the total phenolic acids (75.1%) and favored Eucalyptol and 1-octen-3-ol accumulation (+25.9 and +15.1%, respectively). The here presented comprehensive and multifactorial assessment of the productive and qualitative response of basil provides evidence of the positive effects (from biomass to specialized metabolites) that can be obtained from the management of the pre-harvest factors in soilless cultivation. In addition, it also highlights the role and constraints of the genetic factor in the observed response. We also discuss the implications of our work considering the impact for the food processing industry. Future research may explore the phenolic acids accumulation as a possible fortification means to extend the pesto sauce shelf life, reducing the need of added antioxidants and thermal processing.