Radish Seeds Research Articles

Bait method to detect Pythium species that grow at high temperatures in hydroponic solutions

Pythium helicoides, P. aphanidermatum and P. myriotylum are important pathogens that cause root rot of several crops in hydroponic culture and in ebb-and-flow irrigation systems. These species belong to a group of Pythium species that can grow at temperatures higher than 40°C. We developed a method for baiting these high-temperature Pythium species and evaluated its practicality to monitor their presence in nutrient solutions. Seeds of cucumber, tomato, radish, hemp, perilla and millet and leaves of bent grass and rose were tested as baits in hydroponic systems. Hemp, perilla and radish seeds and bent grass and rose leaves were more effective than the other baits for Pythium zoospores, and bent grass leaves were the most effective. In a sensitivity test, bent grass leaf traps (BLTs) detected three Pythium species after only a 1 day exposure to suspensions of 40 zoospores per liter of water, and the frequency of detection increased with zoospore density and with baiting period. A temperature of 38°C was optimum for the selective reisolation of the high-temperature Pythium species from the BLTs. The BLT was also tested with inoculated and noninoculated miniature roses that shared a recirculating nutrient solution. The pathogen was detected in the nutrient solution 23 days before the disease spread to the noninoculated roses. In addition, P. helicoides was detected 30 days before the disease was evident in a commercial greenhouse. The baiting method described here will be useful for monitoring high-temperature Pythium species in recirculating hydroponic culture systems.

Isolation, characterization and cloning of a cDNA encoding a new antifungal defensin from Phaseolus vulgaris L. seeds

The PvD1 defensin was purified from Phaseolus vulgaris (cv. Pérola) seeds, basically as described by Terras et al. [Terras FRG, Schoofs HME, De Bolle MFC, Van Leuven F, Ress SB, Vanderleyden J, Cammue BPA, Broekaer TWF. Analysis of two novel classes of plant antifungal proteins from radish ( Raphanus sativus L.) seeds. J Biol Chem 1992;267(22):15301–9], with some modifications. A DEAE-Sepharose, equilibrated with 20 mM Tris–HCl, pH 8.0, was initially utilized for the separation of peptides after ammonium sulfate fractionation. The basic fraction (the non-retained peak) obtained showed the presence of one unique band in SDS–Tricine gel electrophoresis with a molecular mass of approximately 6 kDa. The purification of this peptide was confirmed after a reverse-phase chromatography in a C2/C18 column by HPLC, where once again only one peak was observed and denominated H1. H1 was submitted to N-terminal sequencing and the comparative analysis in databanks revealed high similarity with sequences of different defensins isolated from other plants species. The N-terminal sequence of the mature defensin isolated was used to produce a degenerated primer. This primer allowed the amplification of the defensin cDNA by RT-PCR from mRNA of P. vulgaris seeds. The sequence analysis of the cloned cDNA, named PVD1, demonstrated 314 bp encoding a polypeptide of 47 amino acids. The deduced peptide presented high similarity with plant defensins of Vigna unguiculata (93%), Cicer arietinum (95%) and Pachyrhizus erosus (87%). PvD1 inhibited the growth of the yeasts, Candida albicans, Candida parapsilosis, Candida tropicalis, Candida guilliermondii, Kluyveromyces marxiannus and Saccharomyces cerevisiae. PvD1 also presented an inhibitory activity against the growth of phytopathogenic fungi including Fusarium oxysporum, Fusarium solani, Fusarium lateritium and Rizoctonia solani.

The Induction for Adverse Temperature Tolerance During the Germination of Radish Seeds

The changes of adverse temperature tolerance during the germination of radish seeds pretreated by different temperatures were studied.Results showed that the vigor of germination remarkably decreased when the seeds were germinated under the temperatures of higher than 35℃ or lower than 15℃.The mild temperature stress could improve the germination ability of radish seed greatly in high temperature(35℃).The optimal pretreatment low and high temperature are 15℃ and 25℃ respectively.The optimal pretreatment time is 6 hours(at 15℃).The pretreatment of high temperature(at 30℃,12 hours) also could improve the seed vigor in low temperature subsequently,which shows that the seeds have cross adaptation for heat and chilling stresses.

1H Chemical Shift Assignments and Interproton 3JNHA Coupling Constants of Raphanus sativus Antifungal Protein 1 (Rs-AFP1), a Plant Defensin Isolated from Seeds of Radish

1H Chemical Shift Assignments and Interproton 3JNHA Coupling Constants of Raphanus sativus Antifungal Protein 1 (Rs-AFP1), a Plant Defensin Isolated from Seeds of Radish

First Report of Stemphylium vesicarium as Causal Agent of Wilting and Root Rotting of Radish Sprouts in Italy.

A consistent contamination from a Stemphylium sp. was detected on radish (Raphanus sativus) seeds by a seed blotter test. Twenty-five percent of seed lots were contaminated. Stemphylium vesicarium (teleomorph Pleospora allii) was identified on the basis of morphological characters of conidia and conidiophores (4). Conidia were golden brown to dark drown, oblong to oval with one to four transverse and one to three longitudinal septa, constricted at one to three of the major transverse septa. Conidia dimensions ranged from 12 to 22 × 30 to 40 μm. Conidiophores were straight or occasionally one-branched with a swollen apex and one to four septate. Pseudothecia with asci and ascopores were observed on radish seeds. Asci were cylindrical to clavate with eight ascospores with up to six transverse septa and numerous longitudinal septa. Species identification was also confirmed after comparing the sequences of the internal transcribed spacer (ITS) region of rDNA and gpd (glyceraldehyde-3-phosphate dehydrogenase) (3) of four isolates with those of Stemphylium species already present in the NCBI database. Accessions Nos. AM 746020 to AM746023 and AM883174 to AM883177 were deposited for ITS and gpd, respectively. Artificial inoculations were carried out on radish seeds previously disinfected with 1% sodium hypochlorite for 10 min and then plated on S. vesicarium sporulating colonies grown on potato dextrose agar (PDA). The four sequenced isolates were tested for pathogenicity. Disinfected seeds were plated onto PDA only and used as a control. After 48 h of incubation, seeds were sown in sterilized soil in plastic plates. The emerging and the eventually dead plants were counted. Stem necrosis and root rotting developed on sprouts within the first week after sowing. On the surviving infected plantlets, wilting and death occurred on more than 70% of the plants within 4 weeks after sowing. Control plantlets obtained from disinfected seeds remained healthy. The fungus reisolated from wilted and dead plants was morphologically identical to the original isolates, thus confirming S. vesicarium as the causal agent. In Italy, this pathogen is common on asparagus (1), but it has also been reported on Allium spp., tomato, and pear. On European pear it is the causal agent of brown spot (2), a destructive disease in the Mediterranean area but also in the Netherlands and other continental European countries. On the basis of these results, seed contamination with S. vesicarium can represent a threat for the production of radish for sprout consumption. To our knowledge, this is the first report of S. vesicarium on radish plantlets in Italy.

Chemical Stress Induced by Heliotrope (Heliotropium europaeum L.) Allelochemicals and Increased Activity of Antioxidant Enzymes

The aims of this study were to evaluate the allelopathic potential of heliotrope on some biochemical processes of dodder. The preliminary experiments revealed that the effect of aqueous extract of leaves of heliotrope is higher than its seeds and roots. So, the aqueous extract of leaves was used in remaining experiments. Leaf extracts of 5 g powder per 100 mL H2O inhibited the germination of dodder seeds up to 95% and that of radish up to 100%. While, the aqueous extract of vine leaves which is a non-allelopathic plant did not have any inhibitory effect on these seeds. Vine leaf was used as a control to show that the inhibitory effect of heliotrope is due to an inhibitory compound but not due to the concentration. The leaf extract of heliotrope at 0.0, 0.1, 1.0, 2, 3, 4 and 5 g powder per 100 mL H2O reduced the radish seedling growth from 14 cm to about 0.5 cm and that of dodder from 7.5 cm to about 0.25 cm. The effects of heliotrope allelochemicals on some physiological and biochemical processes of radish was also Investigated. The activity of auxin oxidase increased in leaves and roots of radish. Suggesting that the reduced radish growth is due to the decreased active auxin levels in its leaves and roots. The activity of alpha-amylase was reduced, so reduction of starch degradation and lack of respiratory energy is the prime reason of germination inhibition in dodder and radish seeds. The level of soluble sugars increased. This is an indication of reduction of the activity of some respiratory enzymes and reduced consumption of these sugars. Proline levels were also increased, indicating that, the chemical stress is induced by leaf extract. Finally, the activities of GPX and CAT which are antioxidant enzymes were increased, along with increased extract concentration. These finding shows that the chemical stress induced by leaf extract produces super oxide (O2*) and H2O2, which is neutralized to H2O and O2 by these enzymes.

Food safety evaluation of broccoli and radish sprouts

Three cultivars of broccoli seeds (Brassica oleracea var. italica), cv. Tiburon, cv. Belstar and cv. Lucky, and two cultivars of radish seeds (Raphanus sativus), cv. Rebel and cv. Bolide, were germinated for three and five days and safety aspects such as microbiological counts and biogenic amines were investigated. Cytotoxicity evaluation was also carried out. Broccoli and radish sprouts contained numbers of mesophilic, psychrotrophic, total and faecal coliform bacteria which are the usual counts for minimally processed germinated seeds. Putrescine, cadaverine, histamine, tyramine, spermidine and spermine increased during sprout production although these levels were below those permitted by legislation (5mg/100g of edible food). Broccoli and radish sprouts demonstrated no toxic effects on proliferation and viability of HL-60 cells and should be included in our diets as healthy and safe fresh foods.

Quality Characteristics of Fresh Plant Sprouts and After Their Short-term Storage

Quality Characteristics of Fresh Plant Sprouts and After Their Short-term Storage Sensory characteristics, carotenoids content and antioxidant activity of sprouted seeds of eight plant species were investigated. Seeds of lentil, broccoli, clover, amaranth, wheat, radish, pea, and alfalfa were germinated for 3 days in the dark, at the temperature of 25°C and then sprouted seeds were stored for the next 2 days in a refrigerator, in the dark, at the temperature of 4-5°C. Sensory quality was evaluated in the expert panel, with the quantitative descriptive analysis method (QDA), using 15 sensory attributes, concerning odour, texture and flavour. Radish, alfalfa and lentil sprouts showed the highest overall sensory quality. Sensory attributes of sprouts were strongly differentiated among the species and the biggest differences were found for spicy flavour and sweet taste attributes. 2-day storage of sprouts affected some taste/flavour descriptors. Total carotenoids and β-carotene content were the highest in broccoli and radish sprouts. During the storage of sprouts carotenoids content decreased. Antioxidant activity was differentiated between species, and both radish and broccoli sprouts showed the highest antioxidant activity.

채소 종자별 온탕침지 종자소독 효과검정

본 연구는 유기농 채소종자의 소독방법으로 가장 적합한 온탕침지법을 개발하고자 수행하였다. 엽채류 중 열에 비교적 민감한 상추 종자의 최적 온탕침지 조건은 <TEX>$45^{\circ}C$</TEX> 25분이었으며 배추와 무 종자 <TEX>$50^{\circ}C$</TEX> 25분이었다. 온탕침지로 이들 종자의 발아율과 생장은 무처리와 비슷하거나 촉진되었고, Alternaria, Aspergillus, Penicillium, Mucor 등의 종자감염 곰팡이는 90% 이상 혹은 100% 억제되었으며 상추 종자에 감염된 세균 억제효과는 98.5%였다 오이, 고추, 호박 등 과채류 종자 중에는 호박이 가장 온수에 감수성이었는데 <TEX>$50^{\circ}C$</TEX> 15분 침지가 적합하였고 오이와 고추 종자는 <TEX>$50^{\circ}C$</TEX> 25분 처리가 가장 효과적이었다. 온탕 침지한 오이 및 호박 종자의 발아율 및 생장은 무처리와 비슷하거나 약간 높았는데, 고추의 경우에는 발아율은 50% 이상 증가되었으며 뿌리생장은 5배 이상 신장되었다. 이들 종자에 감염된 곰팡이 중 Rhizopus, Aspergillus, Penicillium, Mucor등의 억제율은 <TEX>$72%{\sim}95.4%$</TEX>였고 감염세균의 억제율은 <TEX>$65.5%{\sim}86.0%$</TEX>였다. 본 시험의 결과로 볼 때 온탕침지의 최적 조건은 종자에 따라 다소 다르며, 온탕침지는 모든 종자전염성 병원균을 방제할 수는 없으나 이들을 효과적으로 억제할 수 있는 기술로 유기농업에서 유용하게 활용할 수 있을 것으로 기대된다. Hot water treatment that is the most appropriate seed disinfection method for organic vegetable farming was evaluated in this study. Among the leafy vegetable seeds lettuce that was the most sensitive to hot water was suitable to treat at <TEX>$45^{\circ}C$</TEX> for 25 min, while Chinese cabbage and radish seeds were optimally treated at <TEX>$50^{\circ}C$</TEX> for 25 min. The treatments resulted in similar or higher seed germination rate than non-treated seeds and promoted plant growth. In addition, fungi such as Alternaria, Aspergillus, Penicillium, or Mucor grown on the seeds were suppressed over 90% and the bacterial growth on lettuce seeds reduced 98.5% by the treatment. Among the fruit vegetable seeds pumpkin that was vulnerable to hot water was suitable to treat at <TEX>$50^{\circ}C$</TEX> for 15 min, while cucumber and hot pepper seeds revealed optimum treatment at <TEX>$50^{\circ}C$</TEX> for 25 min as chinese cabbage and radish. The treatment also showed similar or higher seed germination rate and growth than non-treated seeds. Furthermore, fungi such as Rhizopus, Aspergillus, Penicillium or Mucor grown on the seeds reduced from 72.0% to 95.4%. The bacterial growth on cucumber and red pepper seeds was suppressed from 65.5% to 86.0% by the treatment. Results indicated that the hot water treatment is practical for disinfection of organic vegetable seeds and the optimum temperature and soaking time varied among the seeds.

Do plants ever compete for space?

The term “competition for space” occurs often in ecological literature, but there has never been a direct demonstration of this competition. In fact it has been shown that plant canopies are mainly empty of plant organs. F.E. Clements recognized this. He suggested that one of the few situations in which competition for space would occur was in an unthinned row of radishes. Even this has never been demonstrated. We sowed radishes (Raphanus sativa) in a dense row and let them grow to maximum size in favourable conditions. For comparison, the same number of radish seeds were sown in a double, offset row. Squeezing the radish plants into one row was largely compensated by sideways movement of their hypocotyl/taproots, but total plant mass was reduced by 7.5%. Even this cannot be definitely attributed to competition for space. We conclude that competition for space, if it exists at all, is only a small effect even in conditions very favourable for it. It can, as Clements said, be ignored for natural communities.

항 곰팡이 단백질 유전자 분석에 의한 국내 무 품종간 유연성에 관한 연구

국내 시판 무 (Baekwoon) 의 씨앗으로부터 항 진균 단백질들을 (RAP-l,2)분리 하였으며[12] 이들 항 진균 단백질을 MALDI-TOF실험결과, 2S storage albumin, Rs-AFP등 지하부 식물의 defensin protein과[15] 일치함을 확인하였고 이에 시판되는 7종의 각각의 무 씨앗으로부터 조 단백질과 Total RNA를 분리 하여 항 효모 (Saccharomyces cerevisiae, Candida albicans.) 및 항 곰팡이 (Botrytis cenma)에 대한 항 진균성을 실험한 결과 항 곰팡이 활성은 모든 품종에서 보였으나 항 효모 활성은2 종 (Myungsan, Baekwoon) 의 무에서만 보였 다 . 또한 기존에 알려진 항 진균 단백질 (Rs-AFP)의 유전자를 Gene Bank/EMBL 에서 획득하여 씨앗으로부터 분리한 Total RNA 에 RT-PCR 한결과, 7종 중 2종은 0.2kb 의 산물이 보이지 않았다. 이들 Ks-AFP 유전자산물을 염기서열을 분석하였으며 이 염기서열에서 얻어진 아미노산 서열을 Clustal W를 이용한 pairwise alignment 분석에 의해 국내 시판 무 의 품종간 각clone의 계통수를 분석한 결과를 보고한다. To define diversity of domestic radish, we analysis genetic relationship of anti-fungal protein genes from several domestic radish (Raphanus sativus L.) seeds. We have isolated from domestic radish (Baekwoon) anti-fungal protein named RAP[12]. In this report, we isolate RNAs and raw protein from radish seeds then, RT-PCR analysis was done with another known anti-fungal sequences of radish from Gene Bank/EMBL and anti-fun- gal, anti-yeast activity were done against Bot교tis cenerea, Saccharomyces cerevisiaeι Candida albicans with it's raw proteins. The anti-fungal activity was shown used all seeds but anti-yeast activity was shown only two seeds (Myungsan, Baekwoon). RT-PCR products (about 0.2 Kb) were not shown only two seeds. To identify the sequencing relationship of the domestic radish, we have cloned and sequenced RAP genes of the radish and analysis the sequence relationship with clustalw program. Thus we report the result that there are some different relationship between domestic radish and known other radish's anti- fungal protein[15].

Comparative study between using natural and synthetic zeolites for the improvement of soil salinity and crop yield

The effects of natural zeolite (clinoptilolite, NZ) and synthetic zeolite (Y, SZ) on salinity and the presence of the harmful salts in the soil were compared on Raphanus sativus L. Nine soil treatments were studied: 1 (control), 2 (NaCl), 3 (Na2SO4), 4 (NZ), 5 (SZ), 6 (NZ + NaCl), 7 (SZ + NaCl), 8 (NZ + Na2SO4), and 9 (SZ + Na2SO4). Five radish seeds were planted in each pot. Each treatment was repeated 10 times. After 50 days under equal conditions some parameters consisting of the number of leaves (NL), total leaf area (TLA), total fresh weight (TFW), total dry weight (TDW), root fresh weight (RFW), air fresh weight (AFW), root dry weight (RDW) and air dry weight (ADW) were determined. Results showed that adding either NZ or SZ to salinic soil (treatments NZNaCl, NZNa2SO4, SZNaCl and SZNa2SO4) increased AFW, TFW and TDW. Using both NZ and SZ in normal soil increased the weight of plants by developing AFW. Further NZ was more effective on soil with NaCl than SZ.

Nutritional and sensory quality of selected sprouted seeds

The aim of this study was to examine changes of nutritional and sensory quality of sprouted alfalfa (Medicago sativa, L.) and radish (Raphanus sativus L.) seeds treated by high pressure during storage. Along with this, microbiological safety was also observed. Sprouted alfalfa and radish seeds in citric acid pickle, packed in transparent laminated bags PA/PE 80, were treated with 500 MPa pressure for 10 min. The processed seeds in bags were stored in a refrigerator for 21 days. The bags were sampled in regular intervals to perform analyses. Changes in the content of vitamin C, riboflavin, niacin and pantothenic acid were observed during storage in sprouted seeds. The content of carotenoids was also observed in sprouted radish seeds.

ÍNDICES DE FITOTOXICIDAD EN RESIDUOS ORGÁNICOS DURANTE EL COMPOSTAJE

Key words: Bioassays, germination index, compost.ABSTRACTThe maturity of compost can be determined using sensitive species to phytotoxic metabolites by germination bioassays. In order to obtain a stabilized and non-phytotoxic product, the phytotoxic compounds must be biodegraded or immobilized during the maturity phase of composting. In this study the sensitivity of lettuce (Lactuca sativa, var. Four Seasons) and radish (Raphanus sativus, var. Cherry Bell) seeds to extracts from composted agroindustrial residues was evaluated.E The extracts were obtained by mixing agroindustrial residues with distilled water in a ratio of 1:5. The agroindustrial residues evaluated were residues of instantaneous coffee (C), a mixture of residues of instantaneous coffee and fresh fruit residues (C+F) and wine residues with broiler manure residues (V+G). The relative germination rates (PGR), relative growth of radicle (CRR) and germination index (IG) were determined for both species. The results showed that radish was more sensitive to phytotoxic compounds than lettuce. V+G showed the highest phytotoxicity for both species according to the germination index. The Chilean Regulation for Compost (INN, 2004) establishes the phytotoxicity level based on the relative germination rate of radish seeds. According to this Regulation only V+G was classified as “phytotoxics free” compost. The germination index was the most sensitive parameter for evaluating the chemical maturity level of composted residues for agricultural use. This index was especially useful for evaluating the phytotoxicity of compost or any organic substrate used for pots. This is due because when plants are grown in pots the effects of phytotoxic compounds are more important than when plants are cultivated in the field.Palabras claves: Bioensayos, indice de germinacion, compost

Novel Anthracene Derivatives Isolated from Rice Hulls of Oryza sativa and Their Growth Inhibitory Activity of Radish Seed.

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.

Teste de envelhecimento acelerado para avaliação do potencial fisiológico de sementes de rabanete

O presente trabalho teve o objetivo de estudar procedimentos para condução do teste de envelhecimento acelerado para determinar o potencial fisiológico de sementes de rabanete. Foram utilizados seis lotes de sementes de rabanete, cultivar Vip Crimson. A avaliação inicial desses lotes consistiu na determinação do teor de água e nos testes de germinação, primeira contagem da germinação, germinação a baixa temperatura, emergência de plântulas (sob diferentes temperaturas). O experimento foi conduzido em duas etapas: na primeira, as sementes foram submetidas ao teste de envelhecimento acelerado tradicional e com uso de solução saturada de NaCl e, na segunda etapa, com uso de solução não saturada de NaCl, todos durante 48, 72 e 96h a 41ºC. O delineamento experimental foi inteiramente casualizado, utilizando-se na primeira etapa seis tratamentos (lotes) e na segunda etapa quatro tratamentos (lotes) com quatro repetições. Dentre os procedimentos adotados no teste de envelhecimento acelerado, os períodos de exposição de 72h a 41ºC com uso de solução saturada de NaCl e de 48h a 41ºC com uso de solução não saturada de NaCl são adequados para avaliação do potencial fisiológico de sementes de rabanete.

An -L-arabinofuranosidase/ -D-xylosidase from immature seeds of radish (Raphanus sativus L.)

The carbohydrate moieties of arabinogalactan proteins (AGPs) are essential for their physiological functions and undergo rapid turnover in vivo. Degradation of the carbohydrate moieties of AGPs seems to occur by concerted action of several glycosidases, among them alpha-L-arabinofuranosidase, beta-D-galactosidase, and beta-D-glucuronidase. Here, a bifunctional alpha-L-arabinofuranosidase/beta-D-xylosidase from immature seeds of radish (Raphanus sativus L.), which hydrolyses alpha-L-arabinofuranosyl residues of the carbohydrate moieties of AGPs, has been cloned by reverse transcriptase-PCR. The gene, designated RsAraf1, contained an open reading frame of 2343 bp (780 amino acids), including a putative signal sequence (33 amino acids) at the N-terminus. RsAraf1 is highly similar to barley alpha-L-arabinofuranosidase/beta-D-xylosidases and belongs to family 3 of the glycosyl hydrolases based on sequence homology. Southern blot analysis revealed that several related genes exist in the radish genome. RsAraf1 is expressed throughout seed development and weakly expressed in young seedlings. It was found that alpha-L-arabinofuranosidase activity in a cell-wall protein fraction prepared from transgenic Arabidopsis plants with enhanced expression of RsAraf1 was significantly higher than that in a wild-type protein fraction; the crude enzyme preparation released L-arabinose from radish AGPs as well as alpha-(1-->5)-arabinan and arabinoxylan. Accordingly, the amount of L-arabinosyl residues in the cell walls of transgenic plants was significantly decreased. These results indicate that RsAraf1 encodes a bifunctional alpha-L-arabinofuranosidase/beta-D-xylosidase and suggest that RsAraf1 is involved in the hydrolysis of the carbohydrate moieties of AGPs in immature radish seeds.

New Stigmastane Steroids Constituents from Rice Hulls of Oryza sativa and Inhibitory Activity on Radish Seed

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.

Effect of Accelerated Ageing on Seeds of Radish (Raphanus sativus L.)

Effect of Accelerated Ageing on Seeds of Radish (Raphanus sativus L.)

Plants grow better if seeds see green

We report on the response of dry plant seeds to their irradiation with intense green light applied at biostimulatory doses. Red and near-infrared light delivered by lasers or arrays of light emitting diodes applied at such doses have been shown previously by us to have effects on mammalian cells. Effects include cell proliferation and elevation of cell vitality, and have practical applications in various biomedical fields. Growth processes induced by photoreceptor stimulation (phytochromes and cryptochromes) in plant seeds with green light were described so far only for imbibed seeds. In this paper, we show that irradiation of dry cress, radish and carrot seeds with intense green light (laser or arrays of light emitting diodes), applied at biostimulatory doses, resulted in a significant increase in biomass--14, 26, and 71 days after seeding, respectively. In the case of radish and carrot, the irradiation led to important changes in the root-to-foliage surface ratio. Seeds with a potential to grant growth acceleration could be of special interest in agricultural applications, and could even compensate for shorter growth seasons caused by climate change.