ABSTRACT The forthcoming generation of radio telescope arrays promises significant advancements in sensitivity and resolution, enabling the identification and characterization of many new faint and diffuse radio sources. Conventional manual cataloguing methodologies are anticipated to be insufficient to exploit the capabilities of new radio surveys. Radio interferometric images of diffuse sources present a challenge for image segmentation tasks due to noise, artifacts, and embedded radio sources. In response to these challenges, we introduce Radio U-Net, a fully convolutional neural network based on the U-Net architecture. Radio U-Net is designed to detect faint and extended sources in radio surveys, such as radio haloes, relics, and cosmic web filaments. Radio U-Net was trained on synthetic radio observations built upon cosmological simulations and then tested on a sample of galaxy clusters, where the detection of cluster diffuse radio sources relied on customized data reduction and visual inspection of Low-Frequency Array Two metre Sky Survey (LoTSS) data. The 83 per cent of clusters exhibiting diffuse radio emission were accurately identified, and the segmentation successfully recovered the morphology of the sources even in low-quality images. In a test sample comprising 246 galaxy clusters, we achieved a 73 per cent accuracy rate in distinguishing between clusters with and without diffuse radio emission. Our results establish the applicability of Radio U-Net to extensive radio survey data sets, probing its efficiency on cutting-edge high-performance computing systems. This approach represents an advancement in optimizing the exploitation of forthcoming large radio surveys for scientific exploration.