Aim & Objective-This study has been undertaken to assess the knowledge and awareness about basics of radiation and safety measures in diagnostic radiology among allied health professionals including, Radiographers, and students of Radiology and imaging technology. Materials and Methods: A questionnaire based survey was performed to collect data of knowledge about the radiation awareness and safety measures using in diagnostic radiology, among allied health professionals including radiographers, and students of Radiology and imaging technology. Result- Knowledge about principle of radiation protection was very disappointing, because 30.60% of the participants know the basic principle of radiation protection (Justification, Optimization, and Dose Limit). 43.40% participants said that, ALARA is the basic principle of radiation protection while 26% participants said that, TDS (Time, Distance and Shielding) is the basic principle of radiation protection. Discussion-Majority of participants needs to improve their knowledge about the radiation awareness and safety measure. Keywords: Radiation, Safety, Protection, Radiation awareness.