Radial Bone Research Articles

FARKLI RADİUS BAŞI TİPLERİNİN MORFOMETRİK KARŞILAŞTIRILMASI

The aim of this study is to determine morphometric measurements difference of radial head (RH) types. 49 dry radial bones were included in the study. After determining the types of radius, morphometric measurements were made with digital calliper. The parameters were determined as the length of radius, height of the radius, transverse diameter of RH, anterior-posterior diameter of RH, radial tuberosity transverse diameter and height, length and transverse diameter of ulnar notch. As the result of RH classification, it has been determined that; 20 bones had irregular, 15 bones had oval and 14 bones had round type. In order to on all group average; radius length of RH, the height of RH, transverse diameter of RH, anteroposterior diameter of RH were calculated as 207.99±21.97 mm, 9.45±1.68 mm, 19.94±3.15 mm, 20.20±3.09 mm respectively. In order to on all group aver-age; transverse diameter of radial tuberosity, height of radial tuberosity, the height of ulnar notch, transverse diameter of ulnar notch were 12.07±1.3 mm, 25.36±2.78 mm, 13.86±1.56 mm, 5.27±0.8 mm respectively. Ac-cording to the results of One Way Anova analysis, no statistically significant difference was found between RH types (p>0.05). No statistically significant difference was found between RH types in all parameters.

Testing the Functional Model of Bone Development: Direct and Mediating Role of Muscle Strength on Bone Properties in Growing Youth.

This study examines the functional model of bone development in peri-pubertal boys and girls. Specifically, we implemented a mixed-longitudinal design and hierarchical structural models to provide experimental evidence in support of the conceptual functional model of bone development, postulating that the primary mechanical stimulus of bone strength development is muscle force. To this end, we measured radial and tibial bone properties (speed of sound, SOS), isometric grip and knee extensors strength, bone resorption (urinary NTX concentration), body mass index (BMI), somatic maturity (years from peak height velocity) and skeletal maturity (bone age) in 180 children aged 8–16 years. Measurements were repeated 2–4 times over a period of 3 years. The multilevel structural equation modeling of 406 participant-session observations revealed similar results for radial and tibial SOS. Muscle strength (i.e., grip strength for the radial and knee extension for tibial model) and NTX have a significant direct effect on bone SOS (β = 0.29 and −0.18, respectively). Somatic maturity had a direct impact on muscle strength (β = 0.24) and both a direct and indirect effect on bone SOS (total effect, β = 0.30). Physical activity and BMI also had a significant direct impact on bone properties, (β = 0.06 and −0.18, respectively), and an additional significant indirect effect through muscle strength (β = 0.01 and 0.05, respectively) with small differences per bone site and sex. Muscle strength fully mediated the impact of bone age (β = 0.14) while there was no significant effect of energy intake on either muscle strength or bone SOS. In conclusion, our results support the functional model of bone development in that muscle strength and bone metabolism directly affect bone development while the contribution of maturity, physical activity, and other modulators such as BMI, on bone development is additionally modulated through their effect on muscle strength.

Peak vertical jump power predicts radial bone strength better than hand grip strength in healthy individuals.

Osteoporosis is considered a pediatric disease with geriatric consequences. However, measuring bone strength in children is complex and creates a practical problem for health professionals, teachers and parents. A non-invasive measure of muscle fitness that correlates to bone strength may provide a means to monitor bone strength throughout the lifespan. Therefore, the purpose of this study was to investigate the relationship between common muscle function tests (relative grip strength (RGS), peak vertical jump power (PP)) and bone strength in the radial diaphysis and epiphysis of a healthy population. Healthy participants (n=147 (81 female)) performed a bilateral grip strength test using a hand dynamometer, and a maximal vertical jump test. Peak vertical jump power was calculated from maximal jump height using the Sayer’s equation. Moment of inertia (MoI), cortical area (CoA), cortical bone mineral density (cBMD), and polar strength-strain index (SSIp) were measured using peripheral Quantitative Computed Tomography (pQCT) to determine bone strength parameters at the 66% radial site (predominantly cortical bone). At the 4% site (trabecular bone site), bone mineral content (vBMC.tb), bone mineral density (vBMD.tb), total area (ToA.tb) and bone strength index (BSIc) were measured. Hierarchical multiple regression analyses determined the relationship of each muscle function test for each bone envelope (cortical and trabecular). For the cortical bone measurements: RGS, and PP were both significantly correlated with CoA, MoI, and SSIp. Peak vertical jump power predicted bone strength parameters to a greater extent compared to RGS. For the trabecular bone envelope, RGS was not a predictor of bone strength however peak power was a significant predictor of bone strength parameters. Peak vertical jump power was a significant predictor of bone strength at both trabecular and cortical radial sites. Interestingly PP, a lower limb measurement explained the most variance in the bone strength of the upper limb.

Динаміка якості життя осіб похилого віку з старечою астенією та паркінсонізмом під впливом засобів фізичної терапії у постіммобілізаційному періоді після перелому променевої кістки у типовому місці

In old age, due to the spread of age-related diseases, there are changes in the functioning of many organs and systems due to and against the background of polymorbidity, which affects the quality of life and requires correction. The purpose of the study was to determine the dynamics of the quality of life of elderly people with frailty and parkinsonism under the influence of physical therapy in the post-immobilization period after fracture of the radial bone in a typical place. Material and methods. We examined 27 elderly people with frailty and Parkinson's disease in the early postimmobilization period after fracture of the distal metaepiphysis of the radial bone (in a typical place). Group 1 engaged in the principles of outpatient rehabilitation with a predominance of preformed physical factors and group 2 engaged in a developed program of physical therapy, created in terms of patient-centric model of rehabilitation taking into account the specifics of each polymorbid condition using functional body training, massage, nutrition correction, expansion of social activity. The effectiveness of the program was evaluated in the dynamics before and after a month of implementation based on the results of the comparison of quality of life on the questionnaire "Medical outcomes study short form" (SF-36). 36 points were grouped into eight scales: Physical health: Physical Functioning, Role-Physical, Bodily Pain, General Health and Mental Health, Vitality, Social Functioning, Role-Emotional. Results and discussion. At the initial examination the examined people revealed a significant decrease in the results of all scales of physical and mental components of the quality of life of the questionnaire SF-36. The parameters of both groups did not differ statistically significantly (p> 0.05). At retesting, the difference between the results of the physical component of health in the primary and re-study on the physical functioning scale for individuals in group 1 was 18.9%, group 2 – 29.0%, role-physical – 19.8% and 35.5%, respectively, bodily pain – 12.3% and 33.1%, general health – 25.8% and 30.1%. The improvement of the mental component compared to the initial result on the vitality scales in patients of group 1 was 14,0%, group 2 – 27,4%, social functioning – 17,5% and 41,3%, respectively, role-emotional – 15,0% and 23,8%, mental health – 20,5% and 30,2%. The results of testing on all scales were statistically significantly better in individuals of group 1 compared with the result of group 2 (p <0,05). Conclusion. Elderly patients with polymorbid pathology and traumatic bone injuries require the development of pathogenetically based physical therapy programs taking into account and correcting the specifics of each disease, increases the overall effectiveness of rehabilitation and, consequently, quality of life

The Effects of Honey, Curcuma, Ginger, Nigella sativa and Their Mixture on the Radial Bone Defect Repair in Rat Model: Histological and Biomechanical Study

Honey, curcumin, ginger, and Nigella sativa are known for their antioxidant, immune stimulation properties, and wound healing effects. This study was conducted to determine the effect of these compounds and their mixture on the bone healing of the radial bone defect model in rats. One hundred and ten radial bone defects in 55 healthy male rats were randomly divided into 11 groups (10 rats per group). Groups 1-5 received honey, curcumin, ginger, and Nigella sativa orally by gavage, and groups 6-10 received the same materials locally by rubbing them on the sutured skin. The bone repair effects of these materials were evaluated by histopathology and biomechanical examinations. Using honey, curcumin, ginger, and Nigella sativa exhibited a beneficial effect to promote bone healing in this study. The biomechanical and histopathologic evaluations showed that the honey (oral), the mixture of honey and curcumin (oral), and the mixture of honey and Nigella sativa (topical) groups exhibited significantly better results at 52nd postoperative day compared to the other groups.

Effects of long-term plate fixation with different fixation modes on the radial cortical bone in dogs.

The aim of this study was to examine the effect of long-term locking plate fixation on the cortical bone of the canine radius. Locking compression plates were fixed to the left and right radius in dogs (n = 3). The left radius was fixed with a locking head screw (Locking Plate group, LP). The locking compression plate was compressed periosteally in the right radius using a cortex screw (Compression Plate group, CP). Radial bones from dogs that were euthanized for other purposes were collected as an untreated control group (Control group). After euthanasia at 36 weeks following plate fixation, radial bones were evaluated for bone mineral density and underwent histological analysis. Bone metabolic markers were analyzed by quantitative polymerase chain reaction (qPCR). Statistical analyses were performed for comparisons between groups. The LP group showed no significant difference in bone mineral density after plate fixation, whereas the CP group showed significantly lower bone mineral density. Histological analysis indicated that the number of osteoclasts and rate of empty lacunae increased significantly in the CP group relative to the Control and LP groups. qPCR analysis indicated increased expression of inflammatory cytokines, such as tumor necrosis factor-alpha, interleukin-6, and tumor necrosis factor ligand superfamily member 11 in the CP group, whereas Runt-related transcription factor 2, an osteoblast marker, was similar in all groups. The expression of hypoxia-inducible factor-1α in the CP group was also increased relative to that in the Control and LP groups. Thus, locking plate fixation is a biologically superior fixation method that does not cause implant-induced osteoporosis in the bone in the long term.

Radial shortening, bone grafting and vascular pedicle implantation versus radial shortening alone in Kienböck's disease.

We prospectively compared outcomes of two surgical procedures for Stage II and IIIa Kienböck's disease with negative ulnar variance. Group I (13 wrists) was treated with radial shortening alone, and Group II (14 wrists) with combined radial shortening, bone grafting and implantation of a vascular pedicle. At follow-up 3 to 7 years (mean 4.5) after operation, scores by the Quick version of the Disability of Arm, Shoulder and Hand questionnaire and scores for pain and grip strength were significantly better in Group II, but the differences were rather small. On MRI all patients in Group II had revascularization, but 11 patients in Group I had not. We conclude that combining radial shortening with the revascularization procedure improved revascularization. Clinically, both treatments were efficient, and the additional bone grafting and vascular pedicle insertion did not greatly improve function, although statistically there were differences in several follow-up variables. This study also could not identify the independent role of the three procedures (drilling, grafting and artery insertion) in Group II, because decompression or bone grafting alone may be effective.Level of evidence: II.

Failed Trapeziectomy Treated by Thumb Metacarpal Base Arthrodesis: A Case Series.

Trapeziometacarpal joint arthritis is a prevalent condition with a preponderance to women. Most cases are asymptomatic, but typical symptoms are pain, reduced dexterity, and functional decline. Trapeziectomy is the most common surgical treatment in the United Kingdom for patients who remain significantly symptomatic despite nonoperative measures, and this generally produces acceptable outcomes; however, a proportion of patients remain significantly symptomatic. The authors present a case series of 4 patients who underwent successful thumb metacarpal base to index metacarpal base arthrodesis with either distal radial or iliac crest bone grafting. Three of these patients had persisting symptoms after a primary trapeziectomy and 1 patient had gross subluxation of the thumb related to rheumatoid arthritis. Technically, this is a simple procedure to perform. All patients had improved symptoms with satisfactory functional outcomes and an improved cosmetic appearance of the hand.

Dual Role of Caveolin-1 in β-Catenin Signaling During Fracture Healing Induced by Low-Intensity Pulsed Ultrasound in Rabbits

We did this research to observe the effect of LIPUS on long bone fracture repair and caveolin-1, β-catenin signaling expression in the radius defects of rabbits, to explore its possible molecular mechanisms. 24 male New Zealand rabbits with bilateral radial bone defects were divided into 4 groups randomly, n = 6. The right side had daily LIPUS exposure for 20 minutes, while the left received sham treatment. After 7, 14, 21, 28 days, respectively, fracture healing was observed by X-ray imaging and Dual Energy X-ray Absorptiometry (DXA) scan, specimens were harvested for histology, immunohistochemistry, and gene expression analysis. We found that LIPUS brought forward endochondral ossification, increased the bone callus size without changes in Bone Mineral Density (BMD). The caveolin-1 expression increased first then decreased, while the β-catenin kept growing during the process. These demonstrated that caveolin-1 participated in fracture healing accelerated by LIPUS, which was speculated to play a dual role in β-catenin signaling expression.

The effect of plate fixation on supination and pronation of the feline antebrachium: a model of pancarpal arthrodesis

BackgroundPancarpal arthrodesis is purported to limit supination and pronation of the feline antebrachium. The objective of this study was to investigate whether plate fixation of the radius to the carpus and metacarpus limits supination and pronation of the ulna relative to the radius as a model for pancarpal arthrodesis in the cat. Eight feline cadaveric forelimbs were rotated from supination to pronation in a testing jig and CT (computed tomography) was performed in the neutral, supinated and pronated positions. A locking plate was then secured dorsally to the radius, radial carpal bone and metacarpal III of each of the limbs. CT was repeated in each of the testing positions following plate application. The radius and ulna of the control specimens, and the radius, ulna and plate of the plated specimens were then segmented using software. Alignment of the bones to the radius in the control specimens, and to the plate in the plated specimens was used to compare the changes in degrees of movement of the ulna relative to the radius in dorsal, sagittal and transverse planes.ResultsBased on the results of the paired t test, there was no significant difference in degrees of movement, or total range of motion between control and plated specimens in supinated and pronated testing conditions.ConclusionThe results of this ex-vivo study indicate that under the testing conditions employed, plate fixation of the radius to the carpus and metacarpus does not limit supination and pronation of the feline antebrachium.

THE INFLUENCE OF THE PHYSICAL THERAPY PROGRAM ON THE UPPER LIMB FUNCTIONING INDICATORS IN ELDERLY ADULTS WITH SARCOPENIA AND EXTRAPYRAMIDAL DISORDERS IN THE POST-IMMOBILIZATION PERIOD AFTER THE DISTAL RADIUS METAPHYSEAL FRACTURE

Aim: to evaluate the impact of a comprehensive program of physical therapy on the functioning of the upper extremity in the elderly adults with sarcopenia and extrapyramidal disorders (due to Parkinson's disease) in the postimmobilization period after fracture of the distal metaphysis of the radial bone.
 Material and methods. Twenty seven elderly people with sarcopenia and extrapyramidal disorders in the early post-immobilization period after fracture of the distal metaphysis of the radial bone were examined. They were divided into two groups: 1 (practiced according to the principles of outpatient rehabilitation with a predominance of preformed physical factors) and 2 (engaged in the developed physical therapy program, which was created in terms of patient-centered model of rehabilitation taking into account the specifics of each polymorbid condition with functional training, massage, kinesio taping, nutrition correction). The effectiveness of the program was evaluated by comparing the condition of healthy and injured hands according to the results of goniometry, wrist dynamometry, standard tests and questionnaires Nine-hole peg test, Action Research Arm Test, French Arm Test, ABILIHAND, DASH.
 Results. Subjects found significant dysfunction of the upper extremity in all domains of the International Classification of Functioning, as determined by goniometry of the radial-carpal joint, wrist dynamometry, functional tests Nine-hole peg test, Action Research Arm Test, French Research Arm Test, questionnaires and DASH. The developed pathogenetically substantiated complex program of physical therapy showed a statistically significantly better effect on the indicators of functional capabilities of the forearm and wrist compared to the general outpatient rehabilitation program for all studied indicators (p <0.05).
 Conclusion. Elderly patients with complex pathology need to compile pathogenetically substantiated physical therapy programs taking into account and correcting the specifics of each disease, which increases the overall effectiveness of rehabilitation.

Characteristics of lower limb injuries in non-fatal road traffic accidents: a retrospective analysis of forensic medical examinations

Background. Fractures of the lower limbs are one of the most common injuries in road traffic accidents, however, the frequency and localization of fractures, as well as associated trauma, are not well understood. The purpose of the study was to conduct a retrospective analysis of forensic medical examinations of victims with the road traffic injuries of the lower limbs. Results. The research material was 100 reports of forensic medical examinations of victims with fractures of the femur and/or shin bones resulting from a non-fatal road traffic accidents. The study found that the average age of the victims was 41.5 ± 18.8 years (18 - 81 years). The main contingent of those injured was pedestrians (82%). In victims, the most common injury was polytrauma; closed limb fractures were more common. Hip fractures of various localization were found in 39%, shin fractures - in 59%, ankle fractures - in 12%. Closed head injury with a concussion of the brain of a mild degree (23%), closed fractures of the ribs (3%), self-healing dislocation of the humerus (1%), diaphyseal fractures of the forearm (3%), distal radial bone fractures (7%), and fractures of styloid process of the ulna (2%) were also noted. Conclusions. The main victims of road traffic accidents are pedestrians with polytrauma. The most common localization of fractures of the lower extremities is the diaphysis of the femur and the diaphysis of the shin bones.

Modest Changes in Sex Hormones During Early and Middle Adulthood Affect Bone Mass and Size in Healthy Men: A Prospective Cohort Study.

Bone metabolism in men is in part determined by sex steroid exposure. This is especially clear during puberty and senescence but it remains to be established whether declines in sex steroid levels during young and middle adulthood are associated with changes in bone mass and size. This study investigated changes in bone mineral content (BMC), areal bone mineral density (aBMD), volumetric BMD (vBMD), and bone size in relation to sex steroid levels in 999 young adult men (age 24-46 years) of whom 676 were re-evaluated after a mean period of 12 years. Sex hormone-binding globulin (SHBG) levels were measured using immunoassay, testosterone (T) and estradiol (E2) using liquid chromatography-tandem mass spectrometry (LC-MS/MS), and free fractions were calculated (cFT and cFE2, respectively). Areal bone parameters and BMC were measured at the hip and lumbar spine using dual-energy X-ray absorptiometry (DXA). Radial and tibial vBMD and bone size were determined using peripheral quantitative computed tomography (pQCT). Linear mixed models were used for statistical analyses. With aging, we observed decreases in almost all bone mass and density indices, whereas changes in bone geometry resulted in larger bones with thinner cortices. These changes in bone mass and size appeared related to sex steroid levels. Specifically, decreases in cFT (but not total T) levels were associated with larger decreases in lumbar spine BMC and especially with geometric changes in cortical bone at the tibia. Similarly, decreases in total E2 and cFE2 were associated with larger decreases in bone mass (all sites) and also with some geometric changes. Also increases in SHBG were independently associated with aging-related changes in bone mass and size in these men. In summary, even small changes in T, E2, and SHBG levels during young and middle adulthood in healthy men are associated with changes in bone mass and size. © 2022 American Society for Bone and Mineral Research (ASBMR).

Effectiveness of a biodegradable 3D polylactic acid/poly(ɛ-caprolactone)/hydroxyapatite scaffold loaded by differentiated osteogenic cells in a critical-sized radius bone defect in rat.

The effects of a scaffold made of polylactic acid, poly (ɛ-caprolactone) and hydroxyapatite by indirect 3D printing method with and without differentiated bone cells was tested on the regeneration of a critical radial bone defect in rat. The scaffold characterization and mechanical performance were determined by the rheology, scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, and Fourier transform infrared spectrometry. The defects were created in forty Wistar rats which were randomly divided into the untreated, autograft, scaffold cell-free, and differentiated bone cell-seeded scaffold groups (n = 10 in each group). The expression level of angiogenic and osteogenic markers, analyzed by quantitative real time-polymerase chain reaction (in vitro), significantly improved (p < 0.05) in the scaffold group compared to the untreated one. Radiology and computed tomography scan demonstrated a significant improvement in the cell-seeded scaffold group compared to the untreated one (p < 0.001). Biomechanical, histopathological, histomorphometric, and immunohistochemical investigations showed significantly better regeneration scores in the cell-seeded scaffold and autograft groups compared to the untreated group (p < 0.05). The cell-seeded scaffold and autograft groups did show comparable results on the 80th day post-treatment (p > 0.05), however, most results in the scaffold group were significantly higher than the untreated group (p < 0.05). Differentiated bone cells can enhance bone regeneration potential of the scaffold.

Kinematics of the Feline Antebrachiocarpal Joint from Supination to Pronation.

Cats rely on their forelimb mobility for everyday activities including climbing and grooming. Supination and pronation of the forelimb in cats are considered to primarily involve the antebrachium, rather than the carpus. Therefore, our null hypothesis was that there would be no movement of the carpal bones (radial carpal bone, ulnar carpal bone and accessory carpal bone) relative to the ulna during supination and pronation. Eight feline cadaveric forelimbs were rotated from supination to pronation in a jig and computed tomography was performed in the neutral, supinated and pronated positions. The individual carpal bones were segmented from computed tomography images of the supinated and pronated scans in each of the eight specimens. A feline ulna coordinate system was established and used to quantify the translations and rotations between bones of the proximal carpal row and antebrachium. After the carpus was rotated from the initial supinated position into pronation, there was significant translation (x, y and z axes) and rotation (x and y axes) of the proximal row of carpal bones based on absolute magnitude values. Given the differences in translations and rotations of the proximal row of carpal bones, our null hypothesis was rejected. The proximal row of carpal bones translate and rotate independently from the ulna in the cat during pronation of the antebrachium. This may have future implications in the diagnosis and management of feline carpal injuries involving the antebrachiocarpal joint.

Regional differences in the three-dimensional bone microstructure of the radial head: implications for observed fracture patterns.

A characterization of the internal bone microstructure of the radial head could provide a better understanding of commonly occurring fracture patterns frequently involving the (antero)lateral quadrant, for which a clear explanation is still lacking. The aim of this study is to describe the radial head bone microstructure using micro-computed tomography (micro-CT) and to relate it to gross morphology, function and possible fracture patterns. Dry cadaveric human radii were scanned by micro-CT (17μm/pixel, isotropic). The trabecular bone microstructure was quantified on axial image stacks in four quadrants: the anterolateral (AL), posterolateral (PL), posteromedial (PM) and anteromedial (AM) quadrant. The AL and PL quadrants displayed the significantly lowest bone volume fraction and trabecular number (BV/TV range 12.3-25.1%, Tb.N range 0.73-1.16mm-1) and highest trabecular separation (Tb.Sp range 0.59-0.82mm), compared to the PM and AM quadrants (BV/TV range 19.9-36.9%, Tb.N range 0.96-1.61mm-1, Tb.Sp range 0.45-0.74mm) (p = 0.03). Our microstructural results suggest that the lateral side is the "weaker side", exhibiting lower bone volume faction, less trabeculae and higher trabecular separation, compared to the medial side. As the forearm is pronated during most falls, the underlying bone microstructure could explain commonly observed fracture patterns of the radial head, particularly more often involving the AL quadrant. If screw fixation in radial head fractures is considered, surgeons should take advantage of the "stronger" bone microstructure of the medial side of the radial head, should the fracture line allow this.

Effect of vascularized periosteum on revitalization of massive bone isografts: An experimental study in a rabbit model.

In the last years, limb salvage has become the gold standard treatment over amputation. Today, 90% of extremity osteogenic sarcomas can be treated with limb salvage surgery. However, these reconstructions are not exempt from complications. Massive allografts have been associated to high risk of nonunion (12-57%), fracture (7-30%) and infection (5-21%). Association of vascularized periosteum flap to a massive bone allograft (MBA) has shown to halve the average time of allograft union in clinical series, even compared to vascularized fibular flap. Creeping substitution process has been reported in massive allograft when periosteum flap was associated. However, we have little data about whether it results into allograft revitalization. We hypothesize that the association of a periosteum flap to a bone isograft promotes isograft revitalization, defined as the colonization of the devitalized bone by new-form vessels and viable osteocytes, turning it vital. Forty-four New Zealand white male rabbits underwent a 10 mm segmental radial bone defect. In 24 rabbits the bone excision included the periosteum (controls); in 20 rabbits (periosteum group) bone excision was performed carefully detaching periosteum in order to preserve it. Cryopreserved bone isograft from another rabbit was trimmed and placed to the defect gap and was fixed with a retrograde intramedullar 0.6 mm Kirschner wire. Rabbits were randomized and distributed in 3 subgroups depending on the follow-up (control group: 5 rabbits in 5-week follow up group, 8 rabbits in 10-week follow-up group, 7 rabbits in 20-week follow-up group; periosteum group: 5 rabbits in 5-week follow up group, 7 rabbits in 10-week follow-up group, 7 rabbits in 20-week follow-up group). Fluoroscopic images of rabbit forelimb were taken after sacrifice to address union. Each specimen was blindly evaluated in optical microscope (magnification, ×4) after hematoxylin and eosin staining to qualitative record: presence of new vessels and osteocytes in bone graft lacunae (yes/no) to address revitalization, presence of callus (yes/no) and woven bone and cartilage tissue area (mm2 ) to address remodeling (osteoclast resorption of old bone and substitution by osteoblastic new bone formation). No isograft revitalization occurred in any group, but it was observed bone graft resorption and substitution by new-formed bone in periosteum group. This phenomenon was accelerated in 5-week periosteum group (control group: 49.5 ± 9.6 mm2 vs. periosteum group: 34.9 ± 10.4 mm2 ; p = .07). Remodeled lamellar bone was observed in both 20-week groups (control group: 6.1 ± 6.3 mm2 vs. periosteum group: 5.8 ± 3.0 mm2 , p = .67). Periosteum group showed complete integration and graft substitution, whereas devitalized osteons were still observed in 20-week controls. All periosteum group samples showed radiographic union through a bone callus, whereas controls showed nonunion in eight specimens (Union rate: control group 60% vs. periosteum group 100%, p = .003). Association of vascularized periosteum to a massive bone isograft has shown to accelerate bone graft substitution into a newly formed bone, thus, no bone graft revitalization occurs.

Role of bone 1stem cell-seeded 3D polylactic acid/polycaprolactone/hydroxyapatite scaffold on a critical-sized radial bone defect in rat.

Osteoconductive biomaterials were used to find the most reliable materials in bone healing. Our focus was on the bone healing capacity of the stem cell-loaded and unloaded PLA/PCL/HA scaffolds. The 3D scaffold of PLA/PCL/HA was characterized by scanning electron microscopy (SEM), rheology, X-ray diffraction (XRD), and Fourier transform-infrared (FT-IR) spectroscopy. Bone marrow stem cells (BMSCs) have multipotential differentiation into osteoblasts. Forty Wistar male rats were used to organize four experimental groups: control, autograft, scaffold, and BMSCs-loaded scaffold groups. qRT-PCR showed that the BMSCs-loaded scaffold had a higher expression level of CD31 and osteogenic markers compared with the control group (P < 0.05). Radiology and computed tomography (CT) scan evaluations showed significant improvement in the BMSCs-loaded scaffold compared with the control group (P < 0.001). Biomechanical estimation demonstrated significantly higher stress (P < 0.01), stiffness (P < 0.001), and ultimate load (P < 0.01) in the autograft and BMSCs-loaded scaffold groups compared with the untreated group and higher strain was seen in the control group than the other groups (P < 0.01). Histomorphometric and immunohistochemical (IHC) investigations showed significantly improved regeneration scores in the autograft and BMSCs-loaded scaffold groups compared with the control group (P < 0.05). Also, there was a significant difference between the scaffold and control groups in all tests (P < 0.05). The results depicted that our novel approach will allow to develop PLA/PCL/HA 3D scaffold in bone healing via BMSC loading.

Morphometric analysis of the intercarpal ligaments of the equine proximal carpal bones during simulated flexion and extension of cadaver limbs

Despite many reported cases of carpal lameness associated with intercarpal ligament injuries in horses, the morphometry, movement pattern and general intrinsic biomechanics of the carpus are largely unknown. Using osteoligamentous preparation of the carpus prepared from 14 equine cadaver forelimbs (aged 9.62±4.25years), locomotory simulations of flexion and extension movements of the carpal joint were carried out to observed carpal biomechanics and, thereafter, the limbs were further dissected to obtain morphometric measurements of the medial and lateral collateral ligaments (MLC and LCL); medial and lateral palmar intercarpal ligaments (MPICL and LPICL); intercarpal ligaments between radial (Cr) and intermediate (Ci) carpal bones (Cr-Ci ICL); and intercarpal ligaments between Ci and ulnar (Cu) carpal bones (Ci-Cu ICL). The Cr, Ci, Cu and Ca are held together by a series of intercarpal ligaments and move in unison lateropalmarly during flexion, and mediodorsally during extension with a distinguishable proximo-distal sliding movement (gliding) of Cr and Ci against each other during movement. The mean length of MCL (108.82±9.64 mm) was significantly longer (p=0.042) than LCL (104.43±7.65 mm). The Cr-Ci ICL has a dorsopalmar depth of 37.58±4.14mm and a midpoint width of 12.05±3.09mm and its fibres ran diagonally from the medial side of the Ci in a proximo-palmar disto-dorsal direction (i.e. palmarodistally) to the lateral side of the Cr. The specialized movement of the Cr-Ci ICL, which appeared to be further facilitated by a longer MCL suggest a biomechanical function by which carpal damage may be minimized in the equine carpus.

Rare congenital anomaly among population of the Migration Period (based on excavations in the Eastern Aral region)

This paper aims to introduce into scientific discourse the information on unique pathological features ob-served in the individuals of the Jetyasar archaeological Culture buried in the necropolis of Altyn-Asar 4. In the course of examining the extensive paleoanthropological collection of the human remains (more than 600 individu-als) of the Jetyasar Culture from the excavations of the Kwarism Expedition of the USSR Academy of Sciences in the Eastern Aral region, three cases of a rare skeletal anomaly have been discovered, which is manifested by forearm synostosis. In the modern medical literature, slightly more than 350 of such cases have been reported. Radioulnar synostosis severely restricts the movements of pronation and supination (ulnar adduction and devia-tion) by fixing the radial and ulnar bones in a single possible position. Methods of differential diagnostics have been used in description of the skeletal features, alongside the digital micro-focal radiography and microtomogra-phy. There is evidence to suggest congenital form of the above maldevelopment in the Early Medieval Eastern Aral region. Although this pathology may lead to partial disability, its bearers, attributed in the context of the Je-tyasar Culture, were fully socially adapted and each of them had sufficiently long life by the expectancy of the time. Radioulnar stenosis has been identified in a 30–34-year-old male from the grave no. 326.1 of the burial ground of Altyn-Asar 4l and in two 25–29-year-old females from the burial ground of Altyn-Asar 4r (graves nos. 345 and 454.2). The former case is the earlier one, no later than the 4th c. AD according to the archaeological data. By the context of this multiple-body burial, this individual was married and might have carried the congenital pathology onto his descendants. The burial of the married woman with the same pathology in the burial ground of Altyn-Asar 4r (no. 454.2) was made later in the last third of the AD 6th c., according to the planigraphy and AMS radiocarbon date for a neighbouring kurgan. The latest grave no. 345 of the same burial ground, according to the results of the direct dating (UGAMS#43733 1450  20 years BP), is distant in time from the previous case by one generation and corresponds to the beginning of the 7th c.