Rabbits In Experimental Group Research Articles

Histomorphology of the ovaries of rabbits does during ovulation induced by the combined use of gonadotropins

Hormonal stimulation of folliculogenesis is a general method of increasing reproductive capacity in rabbits; however, studying the safety of the developed protocols remains an urgent scientific task. The use of hormonal agents is due to the physiological characteristics of rabbits, primarily coitus-dependent mechanisms of ovulation. Applying gonadotropin-releasing analogs depends on mature follicles ready for ovulation in the ovary. It, therefore, has limited effectiveness for rabbits of the “zero” cycle that have not yet given birth. Therefore, such females are recommended to use medications based on gonadotropins obtained from the serum of foal mares (eCG) or human chorionic gonadotropin (hCG). There is limited data on the combined use of gonadotropins, especially the long-term administration in different doses. Thus, the research aimed to conduct a morphological evaluation of rabbit's ovaries with combined serum and chorionic gonadotropins to stimulate folliculogenesis before artificial insemination to assess the safety of the proposed dosages and long-term administration. Stimulation of ovulation in rabbits of experimental groups was caused by the combined use of serum and chorionic gonadotropins (eCG – 400 IU; hCG – 200 IU) for experimental groups 1 and 2 at a dose of 40 IU and 24 IU, respectively. Females of the control group were induced to ovulate by subcutaneous injection of 0.2 ml of gonadotropin-releasing hormone analog. Ovaries were collected on the seventh day of gestation after the euthanasia of the rabbit with prior premedication. After dissection and macroscopic examination, the ovaries were fixed in a 10% aqueous solution of neutral formalin. Histological slides were made according to the general method by pouring in paraffin and subsequent staining with hematoxylin and eosin. The research has established that repeated (during five reproductive cycles) combined use of serum and chorionic gonadotropins has dose-dependent effectiveness and can cause dysfunction of ovaries and blood circulation. Pathological changes may acquire a systemic character with the manifestation of critical conditions for the health and life of rabbits. The stimulating effect of the combined use of eCG and hCG was detected at a dose of 40 IU, and the inhibition of folliculogenesis was observed at 24 IU. Since the premises for the hyperandrogenism were found microscopically, it was essential to investigate the level of androgens in the blood of rabbits after using a dose of 24 IU. At the same time, both doses of gonadotropins affect the reactivity of the endothelium, which is manifested by the presence of intravascular vesicles and changes in the rheological properties of blood.

Morphological assessment of ovaries during stimulation of ovulation in rab-bits with equine serum gonadotropin in different doses

Ovulatory stimulation of rabbits during artificial insemination requires the use of hormonal drugs to stimulate maturation of follicles, effective fertilization and full manifestation of female sexual function. For this purpose, gonadotropins or analogues of gonadotropin-releasing hormone are common in the practice of rabbit breeding. The latter have a selective effect on folliculogenesis and, under certain conditions, are not capable of inducing ovulation in rabbits of “zero” reproductive cycle, that is, those that have not given birth. Instead, gonadotropins, in particular serum gonadotropin obtained from foal mares (eCG), have a pronounced effect on folliculogenesis in animals. eCG is a glycoprotein hormone secreted by trophoblastic epithelial cells of fetal origin. In other species, except for horses, it is able to induce the release of both luteinizing and follicle-stimulating hormone. That is why eCG is widely used together with human chorionic gonadotropin to induce follicle growth and ovulation. However, there are data on complications and pathological conditions that may occur with long-term use of eCG, especially in genital tract. Therefore, the goal of our research was the morphological evaluation of the functional compartments of ovaries when using eCG to stimulate reproductive function of rabbits. Schemes of hormonal treatment of females in the conditions of a private rabbit farm, an experiment was conducted, which included the introduction of eCG in different doses (40 IU for animals of experimental group 1 and 25 IU for experimental group 2) during five reproductive cycles. Selection of material for histological studies, its fixation, production and analysis of tissue sections stained with hematoxylin and eosin were performed according to commonly used methods. The complex of morphofunctional changes in the ovaries of the rabbits of experimental groups corresponds to the pathomorphological picture of the syndrome of hyperstimulated ovaries. In our experiment, obtained changes were more pronounced at a dose of 40 IU, using which we additionally detected signs of increased immunoreactivity in body of rabbits, as evidenced by the presence of nodular lymphoid formations in outer theca of secondary and tertiary follicles. Thus, as a safe regimen for the use of eCG for ovulatory stimulation in rabbits, we recommend a dosage of 25 IU for 1–2 reproductive cycles.

Hygienic assessment of the probiotic Bioseven influence on the natural resistance of rabbits pre-clinical researches

Rabbit farming plays an important role in the increase of livestock products, one of the precocious branches of small animal husbandry. From a rabbit and its offspring, more than 100 kg of meat can be obtained per year. The body of rabbits, as well as other agricultural and rural animals. animals, during life is under the influence of abiotic and biotic factors that cause growth depression, changes in the morphological composition of blood, and immunological indicators. The use of biologically active drugs that increase immunological reactivity, stimulating growth, development and resistance deserve attention. The use of probiotic preparations of young animals in the early stages of breeding normalizes the microflora of the gastrointestinal tract, stimulates the synthesis of immunoglobulin IgA, increases its natural resistance, and activates homeostasis. The aim of the work was to carry out a hygienic assessment of the effect of the probiotic Bioseven on the natural resistance of the rabbit's body. The following methods of observation were used to obtain scientific information: biological, hematological, biochemical studies, statistical. The research was conducted on rabbits of the gray giant breed of both sexes. Healthy animals with the appropriate body weight were used in the experiments. Fluctuations in body weight in the respective groups did not exceed ±10.0%. Animals were kept in groups in cages in compliance with sanitary and hygienic requirements. The use of the new domestic biologically active drug (probiotic) Bioseven as a means of preventive action is substantiated in the research paper. The drug has been proven to have a positive effect on the morphological, biochemical and immunological parameters of the blood of animals, increase the natural resistance and growth intensity of rabbits. During morphological studies, it was established that the level of endogenous intoxication of the body of rabbits of experimental groups and controls during the observation period remained stable and amounted to 17.95-18.33% (experimental) and 18.42±0.55 in control, which indicates the absence of a suppressor effects of the probiotic bioseven on the antioxidant properties of the rabbit body in doses: 0.5-5.0 g/head. The economic efficiency of the use of Bioseven consists of: a reduction in the morbidity of animals, an increase in their preservation, an average daily increase in body weight, and therefore an additional increase of 0.300-1.200 kg (depending on the dose), a reduction in the cost of obtaining a unit of production (by 0.5 %) and a decrease in the cost of livestock products per head during the period of the experiment by 5.0–15.0 UAH. Thus, the hygienic evaluation of the effect of the probiotic Bioseven on the natural resistance of the rabbit's body indicates the absence of a suppressive effect of the drug on the metabolic processes of the body of laboratory animals. Key words: rabbit breeding, suppressive effect, probiotic, toxicological characteristics, biochemical, ecologically harmless, complete, food products, of animal origin, food safety, consumer.

Ginsenoside Rg1/ADSCs supplemented with hyaluronic acid as the matrix improves rabbit temporomandibular joint osteoarthrosis

ABSTRACT Objective: To investigate whether and how ginsenoside Rg1/ADSCs supplemented with hyaluronic acid as the matrix can improve rabbit temporomandibular joint osteoarthrosis. Method: Isolate and culture adipose stem cells, measure the activity of differentiated chondrocytes by MTT assay and expression of type II collagen in these cells by immunohistochemistry, in order to evaluate the effect of ginsenoside Rg1 on adipose stem cell proliferation and differentiation into chondrocytes.32 New Zealand white rabbits were randomly divided into four groups: blank group, model group, control group and experimental group, 8 in each group. Osteoarthritis model was established by intra-articular injection of papain. Two weeks after successful model building, medication was given for the rabbits in control group and experimental group. 0.6 mL ginsenoside Rg1/ ADSCs suspension was injected into superior joint space for the rabbits in control group, once a week; 0.6 mL ginsenoside Rg1/ ADSCs complex was injected for the rabbits in experimental group, once a week. Results: Ginsenoside Rg1 can promote ADSCs-derived chondrocytes’ activity and expression of type II collagen. Scanning electron microscopy histology images showed cartilage lesions of the experimental group was significantly improved in comparison with control group. Conclusion: Ginsenoside Rg1 can promote ADSCs differentiate into chondrocytes, and Ginsenoside Rg1/ADSCs supplemented with hyaluronic acid as the matrix can significantly improve rabbit temporomandibular joint osteoarthrosis.

VETERINARY AND SANITARY ASSESSMENT OF THE MEAT FROM ANIMALS AFTER ORAL ADMINISTRATION OF THE ANTIPARASITIC DRUG «DEGELM-16»

The paper presents veterinary and sanitary assessment of the meat from rabbits and pigs, who had taken a new anthelmintic drug «Degelm-16» in their diet. The meat from 6 rabbits and 6 gilts who were divided into experimental and control groups was studied, three samples per each group. The drug «Degelm-16» was given alimentary with foodstuff to the animals of the experimental group 5 days before the slaughter at a dose of 50 mg/kg. Rabbits and gilts of the control group did not receive the drug. It was discovered that meat samples taken in the control and experimental groups of rabbits and gilts contained a drying crust, elastic muscle fibers. The characteristic smell of test samples corresponded to the specimens species. Meat samples from both animal species were of pale pink colour. Boiling of the meat from rabbits and gilts resulted in cloudy-white broth with pleasant specific smell. The benzidine reaction revealed a peroxidase positive result in the meat extract. The amount of amino-ammonia nitrogen in both rabbit groups ranged from 0.54 to 0.60 mg, in gilt groups – from 0.76 to 0.79. The volume of volatile fatty acids in rabbit meat samples ranged from 1.82 to 1.89 mg KOH. In gilt meat – from 2.19 to 2.43 mg KOH of volatile fatty acids. The reaction for the protein primary decomposition products using copper sulphate in both groups resulted in getting the broth without clots and flakes confirming appropriateness of the meat. The touch smears bacterioscopy showed presence of single microorganisms in the surface layers and complete absence of the bacteria in the deep layers. The data of microbiological analysis did not reveal deviations from the reference values.

Влияние витаминизированных кормовых добавок на гематологические показатели и мясную продуктивность кроликов

The article presents the results of studies whose purpose was to study the effect of fortified feed additives «Abiovit» and «Nitamin» on blood parameters (clinical, biochemical) and slaughter yield of rabbits. The studies were carried out on the territory of a personal subsidiary farm located in the Moscow region (Ramensky district) and at the Department of Parasitology and Veterinary and Sanitary Expertise of the Federal State Budgetary Educational Institution of Higher Education MGAVMiB - MVA named after K.I. Scriabin. The objects of the study were rabbits of the Californian breed. Rabbits of the experimental groups were grown using feed additives «Abiovit» and «Nitamin», which were received with water for drinking, at a dosage of 1 ml / 1 liter of water by the course method from 60 and 80 days of age for 7 days each day, the control group did not receive feed additives. When studying hematological blood parameters, it was found that the use of fortified feed additives «Abiovit» and «Nitamin» has a positive effect on the body of rabbits during the period of technological stress, as evidenced by the indicators of the number of erythrocytes and leukocytes in the blood of experimental rabbits, and we also noted a positive effect on synthesis of hemoglobin and stimulation of protein metabolism in the body of rabbits. It should be noted that the use of fortified feed additives does not adversely affect the biochemical parameters of rabbit blood in general. The slaughter yield increased in the experimental groups of rabbits that received fortified feed additives by 1.5–2% compared to the control.

EFFECT OF FEEDING DIFFERENT DOSES OF TRITICAL GRAIN ON THE DEVELOPMENT OF RABBITS INTERNAL ORGANS

The results of the study of the influence of Poliske 7 triticale grain for its addition into the standard granulated compound feed to replace cereal ingredients (barley and oats) at the rate of 100; 50; 25 and 12.5%, respectively are highlighted, in the I, II, III and IV research groups on the massometric indicators of the internal organs of young rabbits of the termon breed with the intensive technology of growing them for meat. It was established that feeding triticale grain as a part of a complete ration granulated compound feed in the period from 50 to 100 days of age to experimental groups of rabbits led to an improvement in the relative indicators of internal organs, compared to the control group.
 It was noted that the addition of triticale grain into the compound feed in the amount of 50 and 12.5% led to changes in the weight of internal organs, the weight of the stomach and the length of the small and large intestine sections compared to the control group, which may indicate a positive effect of triticale grain on young rabbits.
 The mass of the entire alimentary canal was 3.6% greater in the animals of the II experimental group compared to the control. The use of experimental compound feed in rations contributed to an increase in the weight of the empty stomach by 14.7% in rabbits of III and 11.2% of IV experimental groups, the weight of the small intestine with chyme in animals of II, III and IV experimental groups - by 19.6; 10.7 and 3.9% compared to the control, fat mass – by 7.4, respectively; 3.6 and 1.9%.
 Тriticale grain in the amount of 50 and 12.5% of the weight of the grain group contributed to the tendency to increase the edible body parts of young rabbits compared to the control. The weight of the cooled carcass of rabbits of all experimental groups increased by 9.6; 10.2; 3.4 and 14.7%. It should be noted that the mass of pulp in the carcasses of animals of the II and IV groups was higher by 11.1 and 1.5%, and the mass of internal fat was lower by 6.3 and 1.6% compared to the analogues, which indicates a positive the effect of feeding triticale grain as part of granulated compound feed.
 There was a tendency towards an increase in the weight ratio of the liver, lungs and heart of rabbits of all experimental groups compared to the control.

Productivity and slaughter rates of rabbits fed by probiotic supplement probi-ol

The productivity, slaughter parameters and weight of internal organs of fattening young rabbits of the White Giant breed additionally fed by probiotic supplement Probiol containing three types of lactic acid bacteria (Streptococcus faecium, Lactobacillus plantarum, Lactobacillus salius) have been researched. It was found out that additional feeding by probiotic supplement Probiol in the amount of 0.025 % to weight of feed has a positive effect on productivity, survival and slaughter indicators of young rabbits. The second experimental group rabbits have increased live weight by 14.4%, survival by 13%; feed consumption per 1 kg of growth decreased by 12 %. Slaughter rates in experimental group exceeded their counterparts in the control group, which confirms the feasibility of introducing a probiotic supplement to rabbits for fattening.

The effectiveness of the use of the probiotic complex Subtilis-C in the field of industrial rabbit breeding

For the successful development of the livestock industry, including rabbit breeding, it is necessary to reveal the genetic potential of breeding facilities by balancing feed rations and including dietary supplements in their composition that help maintain their homeostasis and reduce the negative impact of various stress and feed factors on the body of farm animals. Currently, there are a number of feed components that stimulate the productivity and growth of animals, birds, and animals. One of them are probiotic drugs that have a positive effect on strengthening immunity, strengthening health and increasing productivity of various types of farm animals. The aim of the work is to substantiate the effectiveness of the use of the probiotic drug Subtilis - C as part of a full-fledged granular compound feed for young rabbits to increase their meat productivity. To study the effect of the probiotic drug Subtilis - C, 45 rabbits aged 30 days were selected. Rabbits of the control group were fed according to the scheme adopted by the farm with a full-grain granular feed PZK-92 UAH, experimental groups of rabbits received feed rations based on the feed PZK-92 (obtained on the basis of cereals, sunflower cake, wheat bran, herbal flour from alfalfa and premix KVP P90-1K), which included probiotic the additive "Subtilis-S" (2 · 109) in a dosage of 0.6 and 1.0 g / kg of compound feed and hydrolysate of oats in a dosage of 2% as a binder instead of molasses. It is proved that the use of the probiotic drug "Subtilis-S" in a dosage of 1.0 g / kg by weight of compound feed increases the average daily gains and, as a consequence, meat productivity and protein content in the muscle tissue of rabbit carcasses.)

Enterocin M in Interaction in Broiler Rabbits with Autochthonous, Biofilm-Forming Enterococcus hirae Kr8 Strain.

Young rabbits are susceptible to gastrointestinal diseases caused by bacteria. Enterococcus hirae can be associated with diseases. But enterocins produced by some enterococcal species can prevent/reduce this problem. Therefore, the interaction of enterocin M with a biofilm-forming, autochthonous E. hirae Kr8+ strain was tested in rabbits to assess enterocin potential in vivo. Rabbits (96), aged 35days, both sexes, meat line M91 breed were divided into four groups, control C and three experimental groups. The rabbits in C received the standard diet, rabbits in experimental group 1 (E1) received 108CFU/mL of Kr8+, a dose 500 µL/animal/day, E2 received Ent M (50 µL/animal/day), and E3 received both Kr8+ and Ent M in their drinking water over 21days. The experiment lasted 42days. Feces and blood were sampled at day 0/1 (at the start of the experiment, fecal mixture of 96 animals, n = 10), at day 21 (five fecal mixtures per group, n = 5), and at day 42 (21days after additives cessation, the same). At days 21 and 42, four rabbits from each group were slaughtered, and cecum and appendix were sampled for standard microbial analysis. Ent M showed decreased tendency of Kr8+. Using next-generation sequencing, the phyla detected with the highest abundance were Firmicutes, Verrucomicrobia, Bacteroidetes, Tenericutes, Proteobacteria, Cyanobacteria, Saccharibacteria, and Actinobacteria. Interaction of Ent M with some phyla resulted in reduced abundance percentage. At day 21, significantly increased phagocytic activity (PA) was found in E1 and E2 (p < 0.001). Kr8+ did not attack PA and did not stimulate oxidative stress. But Ent M supported PA. The prospective importance of this study lies in beneficial interaction of enterocin in host body.

Study on TiO2 Nanofilm That Reduces the Heat Production of Titanium Alloy Implant in Microwave Irradiation and Does Not Affect Fracture Healing.

Background Metal implants can produce heat and damage adjacent tissues under microwave irradiation, which makes local metal implants in the body a contraindication for microwave therapy. However, with the wide application of titanium alloy implants which have low permeability and low conductivity, this concept has been challenged. Our team members have confirmed through previous research that continuous low-power microwave irradiation does not cause thermal damage to the surrounding tissues of the titanium alloy. Is there any other way to further increase the dose of microwave irradiation while reducing the heat production of titanium alloy implants? In this study, the effect of TiO2 nanofilm on reducing the heat production of titanium alloy implants in microwave field was verified by animal experiments, and the effect of TiO2 nanofilm on fracture healing was observed. Methods 30 rabbits were selected. In the experiment of temperature measurement, 10 rabbits were randomly divided into experimental group (n = 5) and control group (n = 5), and the contralateral lower limb of the rabbits in experimental group was set as the sham operation group. The right femurs in the experimental group were implanted with Ti6Al4V plates coated with TiO2 nanofilm, and the right femurs in the control group were implanted with common titanium alloy plates without TiO2 nanofilm. The same surgical procedure was used in the sham operation group, but no plate was implanted. The temperature of the deep tissue above the metal implant was measured with an anti-interference thermocouple thermometer during 20 minutes of microwave irradiation. The other 20 rabbits were randomly divided into two groups, experimental group (n = 10) and control group (n = 10). The femoral shaft fracture models were established again. Ti6Al4V plates coated with TiO2 nanofilm and common titanium alloy plates were implanted in the two groups, respectively, and both groups were exposed to continuous microwave irradiation with a power of 40 W or 60 W for 30 days after operation. The fracture healing was evaluated by X-ray at 0 day, 14 days, and 30 days after microwave irradiation, respectively. The animals were sacrificed at 30 days after operation for histopathological assessment. Results The temperature in the experimental group, control group, and sham operation group increased significantly after 40 W and 60 W microwave irradiation (2.18 ± 0.15°C~6.02 ± 0.38°C). When exposed to 40 W microwave, the temperature rise of the control group was 4.0 ± 0.34°C, which was significantly higher than that of the experimental group 2.82 ± 0.15°C (P < 0.01) and the sham operation group 2.18 ± 0.33°C (P < 0.01). There was no significant difference in temperature rise between the experimental group and the sham operation group (P = 0.21). When exposed to 60 W microwave, the temperature rise of the control group was 6.02 ± 0.38°C, which was significantly higher than that of the experimental group 3.66 ± 0.14°C (P < 0.01) and sham operation group 2.96 ± 0.22°C (P < 0.01), and there was no significant difference between the experimental group and the sham operation group (P = 0.32). X-ray evaluation showed that there was no significant difference in callus maturity between the experimental group and the control group at 14 days (P = 0.554), but there was significant difference in callus maturity between the two groups at 30 days (P = 0.041). The analysis of bone histologic and histomorphometric data at 30 days was also consistent with this. Conclusion Under the animal experimental condition, compared with the common titanium alloy implant, the TiO2 nanofilm can reduce the heat production of the titanium alloy implant in the 2450 MHz microwave field and has no adverse effect on fracture healing. This study opens up a promising new idea for the application of microwave therapy to metal implants in human body.

Study of the Effectiveness of Drug No. 1 on the Model of Alkaline Eye Burn in Rabbits.

Studies have shown that 0.05 mL/kg of drug No. 1 as 1:15 dilution with sterile saline solution has anti-inflammatory, wound-healing, and angioprotective effects if instilled in both eyes of rabbits twice a day for 30 days after an alkaline burn. A stimulation of reparative processes in the cornea was observed with the test dose of drug No. 1. This was manifested by accelerating the recovery of defects in the anterior epithelium and stroma, reducing the frequency of formation of deep defects and the severity of inflammatory reaction and vascularization, and inhibiting the formation of turbidity of its lower intensity and area. A tendency to restore laminarity of the stroma was determined by the action of drug No. 1 throughout the observation period. This contributed to a decrease in the degree of vascularization and prevented ulceration and perforation of the cornea. By the end of experiment, a restoration of strong epithelial-stromal relationships in the experimental group, compared to the control group, was observed due to formation of normal architectonics of fibrous components of intercellular substance. A more pronounced proliferative activity, with an increase in the layering of limbal epithelial cells, was noted in the limbal zone of the cornea in the experimental group rabbits compared to the control group.

Efficiency Of Using Domestic Feed Additive On Biological And Physiological Indicators Of Rabbits

Research has been carried out to study the influence of "ProBioKorm Uz", a biologically active feed additive, on the development and physiological characteristics of rabbits of ‘New Zealand’ breed. During the experiment, 2 experimental and a control groups were formed. Adding "ProBioKorm Uz" to the main feed at the rate of 1% and 2% had an increase in experimental group rabbits’ live weight. Average live weight of them was higher by 5,7 %-3.8% than in the control group. Feed units composed 1.59%, dry matter 0.56 kg (3.61%) and crude protein 0.11 kg (3.59%) compared with the control group. Rabbits of experimental groups had higher indicators of erythrocytes concentration by 0.34-1.00 1012/l (10.34-19.53%), leukocytes - by 0.370-0.46 109/l (5.66-8, 06%), as well as a high level of hemoglobin by 7.42-11.08 g/l (6.81-10.08%) and total protein - by 2.40-4.48 (3.64-6, 42%).

Effect of implants of hydroxyapatite with tricalcium phosphates alloyed with Si on histomorphological and biochemical parameters in cases of bone defects of rabbits

Complex comminuted fractures are accompanied by development of bone defects and loss of reparative potential of the bone tissue in the region of the trauma. This brings the necessity of using implants with optimum osteoconductive and osteointegration properties. The objective of the study was determining the condition of biochemical bone markers and peculiarities of histomorphological changes under the influence of ceramic hydroxyapatite (HA) implants with various physical-chemical properties in the conditions of diaphyseal bone defects in rabbits. We composed control and experimental groups of rabbits with 10 individuals in each with diaphyseal bone defects (3 mm) of the radial bones formed under general anesthesia. In one experimental group, they were filled with granules of hydroxyapatite with α-tricalcium phosphate, and in the second group – with β-tricalcium phosphate, alloyed with Si. In the control rabbits, the defects healed under a blood clot. Blood was analyzed on the 3rd, 7th, 14th, 21st and 42nd days, and as reference we used biochemical parameters of blood of clinically healthy rabbits (n = 10). Bone biopsied materials were taken on days 21–42 under general anesthesia. When using hydroxyapatite with β-tricalcium phosphate, alloyed with Si, we determined early intensification of the levels of nitrogen oxide, angiogenesis and development of bone regenerate in conditions of shortening of inflammatory resorption phase, which was verified according to the level of tartrate-resistant acid phosphatase. According to the level of bone isoenzyme of alkaline phosphatase in the blood serum of animals of the control group, the reparative osteogenesis developed slowly and peaked on day 42, whereas in animals implanted with α-tricalcium phosphate, its development peaked peaked on days 14–42, and when using Si-alloy – on days 7–14. Histomorphologically, on the 21st day, in the case of replacement of bone defect with hydroxyapatite with α-tricalcium phosphate, coarse-fibered type of bone regenerate developed with no dense contact with the elements of the regenerate, while spongy bone trabeculae occurred when hydroxyapatite was applied with β-tricalcium phosphate alloyed with Si, and the control rabbits were observed to be in the stage of cartilaginous callus. On the 42nd day, under the influence of implants of hydroxyapatite with α-tricalcium phosphate, the spongy bone tissue transformed into compact tissue with further mineralization. With implants alloyed with Si, there occurred compact bone tissue, and bone regenerates of the control animals were regions of coarse-fibered and spongy bone tissue without dense contact with the parent bone. This study revealed that hydroxyapatite with β-tricalcium phosphate alloyed with Si had notable osteoinductive and osteointegrating properties, as indicated by early angiogenesis and osteoblast reaction, positive dynamics of the marker biochemical parameters with faster and better development of bone regenerate as spongy bone trabeculae.

Productivity of Young Rabbits at Different Sources of Cuprum in the Mixed Fodder

The impact of different levels and sources of coppermixedligand on productivity and its metabolism in young rabbits of California breed in combination with sulfates was investigated in experiment. For rabbits of the 1st control group 7.81 g/t of copper sulfate was injected into the mixed fodder, of the 2nd experimental group - 7.81 g/t of coppermixedligand, of the 3rd, 4th and 5th experimental groups, respectively, g/t: 5.86; 3.91 and 1.95. As a result, the rabbits of the 1st control group and the 2nd experimental group deficit of copper was eliminated by 100% to the existing norm, and the rabbits of the 3rd, 4th and 5th experimental groups - by 75, 50 and 25%. Studies have shown that during the main period of the experiment, the increase in live weight in all experimental groups exceeded the indicator of the control group. In particular, in rabbits of the 2nd experimental group this exceeding was 9.0% (p < 0.05), 3rd - 13.2% (p < 0.05), 4th -14.0% (p < 0.001) and 5th - by 4.9%. Among all the experimental groups, the best indicators of nutrient digestibility of feed were the rabbits of the 4th experimental group, which in the composition of the mixed fodder fed coppermixedligand, which covered a deficiency of this element by 50% in metal chelate from the amount of Copper of the 2nd experimental group. Compared to control group the digestibility rate of organic feed matter in these animals increased by 3.7%. This increase was due to an increase in the digestibility of crude protein - by 4.5%, crude fat - 0.5, crude fiber - 4.6 and nitrogen-free extractives - 3.7%. According to the results of the experiment, the rabbits of the experimental group 4 ate 5.0% less feed than the counterparts of the 1st control group, and the feed costs were 3.9% lower. The use of the organic form of copper in the form of mixed-ligand complex in mixfeed for rabbits of breeding on meat in the amount of 3.91 g/t or 50% of metalchelate promoted an increase in the growth of animals, nutrient digestibility of feed and reduced feed conversion.

Effect of consumption of I, Se, S and nanoaquacitrates on hematological and biochemical parameters of the organism of rabbits

Using nanotechnologies to obtain compounds of mineral substances is promising because of the importance of the amount of mineral substances, but the limiting factor is particularly their bioavailability in the digestive tract. The conducted studies determined the stimulating effect of nanoaquachelates of separate biogene microelements on the course of biochemical processes of the organisms of animals, their productivity and quality of the obtained products. However, the mechanisms of impact of carboxylates of separate elements and their combinations on functioning of physiological systems of various species of animals, level of transformation of these elements into the products and their biological value remain unstudied. Therefore, the objectives of the study were changes in hematological biochemical and productive parameters of the organisms of rabbits consuming I, Se, S, the impacts of these elements on their organism from the 53 to 85th days of life. Studies were performed on young Termonde rabbits. Animals of experimental groups I, II, III, and IV were fed with feeds of the diet of the control group for 24 h with water containing solution of І, Se, S calculated respectively as 2.5, 5.0, 10.0 and 20.0 µg of І/L of water. The experiment lasted 45 days, including a 12 day preparation period and 33 day experimental. On the 53rd day of life of animals in the preparation period, and 68th and 85th days of life in the experimental periods (15th and 33rd days of watering with supplements), we collected samples of blood from the marginal ear vein of 6 animals (3 males and 3 females) of the group for hematological and biochemical studies. We determined that watering with the solution of microelements І, Se, S led to changes in the overall amount of white blood cells, making it 17.1% lower in the blood of animals of the experimental group II, 26.2% higher in group ІІІ on the 15th day, and 12.0% higher in group IV on the 31st day of the experiment compared with the control. Absolute amounts of lymphocytes, monocytes and granulocytes by the periods of the study were observed to have no significant changes throughout the study, though their parameters were within the physiological values compared with the control. The amount of red blood cells in the blood of rabbits of experimental groups І, ІІ and ІІІ was higher respectively by 8.1%, 5.7% and 12.0% at the first stage of the study and 18.1%, 13.3%, 24.5% and 24.9% higher in animals of experimental groups І, ІІ; ІІІ and ІV at the final stage of the study compared with the control group. Hemoglobin concentration in blood of rabbits of experimental groups II and III during the study was significantly higher. Hematocrit value in blood of rabbits of experimental groups I–III was significantly higher on the 33rd day of the experiment compared with the control. Consumption of solution of microelements in the amount of 10.0 µg of I/L by the animals of experimental group III led to 15.1% higher content of total protein in the blood on the 33rd day of the study compared with the control group of animals. The level of creatinine was no higher than the physiological parameters and was 9.2%, 15.0% and 15.4% higher in the blood of rabbits of experimental groups II, III, IV on the 33rd day of the experiment compared with the control group. No significant changes in the content of triacylglycerols in the blood of rabbits of the experimental groups were seen throughout the study. However, the content of cholesterol in the blood of rabbits of experimental groups III and IV was significantly higher at the first stage compared with the control group. Further, it is practical to study physiologically substantiated amounts of solution of microelements І, Se, S in the diet of mother rabbits to determine their effect on their ability to become pregnant, reproductive function, milk production and survival of offspring.

Influence of Streptomyces levoris CNMN-Ac-01 on meat productivity and chemical parameters of rabbit meat

The purpose of the work was to evaluate the influence of biomass of Streptomyces levoris CNMN-Ac-01 added to fodder on meat productivity and chemical composition of rabbit meat. The experimental and control groups of animals (each one consisting of 5 rabbits) were formed according to analogous principles of age, sex (only females), body weight, mode of maintenance and feeding. The age of rabbits used for the experiment was 45 days. The control rabbits were fed with granulated fodder produced according to the recipe developed in the laboratory of Nutrition and Forage Technologies. The rabbits in experimental group were fed with the same granulated fodder supplemented with 0.1% biomass of Streptomyces levoris CNMN-Ac-01. The results of the chemical analyzes of the used fodder indicated that the elaborated fodder composition provide rabbits with all necessaries for their vital activity, where as added biomass of Streptomyces levoris CNMN-Ac-01 do not significantly affect its chemical composition. The increase of the protein content by 0.20% and water by 1.15% were observed in meat of experimental rabbits where the content of fat was decreased for 0.71% in comparison to those of the experimental group. The supplementing of streptomycete biomass in granulated fodder had an essential effect on the weight gain of rabbits and improves the bone and meat ratio in the carcass.

Influence of vitamin-mineral supplement on protein metabolism in rabbits’ organisms

The purpose of the study is to study the influence of different vitamin-mineral supplement “Tekro” doses on biochemical parameters in blood plasma and liver of rabbits of New Zealand breed. The research methodology is based on research according to generally accepted research methods. Biochemical parameters were studied in rabbits’ organisms at 45, 60, 75 and 90 days of rearing with feeding of different doses of vitamin-mineral supplement “Tekro”, namely 3 %, 3.5 %, 4 %. The academic novelty is that nowadays the problem of the effect of different vitamin-mineral supplement “Tekro” doses on biochemical parameters in the blood and liver of rabbits has not been studied. Analyzing the indicators of the total protein content in the blood plasma of rabbits of the experimental groups and the control one for 90 days, it was noted that there was no significant difference in the indicators. In the liver of rabbits of the age from 45 to 90 days there was a tendency to increase the protein content due to the effects on the body of various doses of vitamin and mineral supplement “Tekro”. It has been established the positive effect of vitamin-mineral supplement “Tekro” on ALT activity of rabbits in experimental groups. At the age of 75 days, significantly higher ALT activity was recorded in rabbits of the 3rd experimental group by 35.8 % compared to animals in the control group.

Analysis of the Correlation Between the Tissue Metabolism of Sodium Alginate Microspheres and the Interventional Therapy of Liver Cancer

To explore the tissue metabolism of sodium alginate microspheres in treating liver cancer using alginate microspheres. Rabbit liver cancer model was established by direct inoculation of liver cancer tissues. Rabbits of experimental group were treated with hepatic arterial embolization (HAE) as follows: sodium alginate microspheres was injected through femoral artery to three targeting sites which was 0.5 cm (proximal group), 2 cm (middle group), and 3.5 cm (distal group) far from the lesion, respectively, to block the tumor’s nutrient supply. Short-term clinical efficacy, 3 month survival rate, 6-month mortality rate, 1-year survival rate, and adverse reactions were used as parameters to analyze the prognosis. The complete remission rates of proximal group, middle group, and distal group was 80%, 40%, and 20%, respectively. The complete remission rate of proximal group was significantly higher than other two groups, and the complete remission rate of middle group was significantly higher than distal group (P &lt; 0.05). In terms of progress, a significant difference was observed between proximal group and distal group (P &lt; 0.01). 3-month and 1-year survival rates were increased along with decrease of distance from injection position to the lesion with a correlation equation of y = −15.567x + 104.47 (R2 = 0.9429) and y = −13.333x + 91.133 (R2 =0.9639). Six-month mortality rate decreased along with the decrease of distance from injection position to the lesion with the correlation equation of y = 15.343x−5.9967 (R2 = 0.9943). The prognosis is correlated with the injection position of alginate microspheres. The closer the injection position to the lesion, the better prognosis.

SLAUGHTER QUALITIES AND BIOLOGICAL VALUE OF RABBIT MEAT FOR FEEDING VITAMIN-MINERAL SUPPLEMENT «TEKRO»

The problem of complete mineral and vitamin nutrition in rabbit breeding is relevant today, despite new discoveries, and research in this direction continues both in Ukraine and around the world. The paper presents experimental data from the results of research to study the effect of different doses of vitamin and mineral supplement "Tekro" in the diets of young rabbits on their productivity, slaughter quality and biological value of rabbit meat. The obtained data of body growth, massometric indicators of carcasses and internal organs of rabbits of experimental groups after slaughter indicate a positive effect of vitamin-mineral supplement "Tekro" on the intensity of development of the body and individual internal organs, which contributes to increased metabolic processes and weight gain in animals research groups. Feeding vitamin and mineral supplements to rabbits of experimental groups of the New Zealand breed for 45 days contributed to a better transformation of feed nutrients into products. In rabbits of the 3rd experimental group, which together with basic ration were fed 3.5 % of the premix "Tekro" at 90 days of age, the highest rates of body weight were recorded, which differed by 8.89 % compared to the control group. In a set of indicators that characterize the nutritional value of rabbit meat, we studied sensory indicators that characterize the quality of rabbit meat: taste, color, pH, juiciness, moisture content, elasticity, aroma, "marbling", etc. It was found that in the appearance of carcasses and muscles in the control group, the samples scored 8.4 points. In the 3-rd and 4-th experimental groups, the number of points on this indicator was higher by 1.2 %. The difference was a trend. Examining the aroma of meat, it was found that in the control of this indicator, the samples scored 7.8 points. In meat samples from experimental groups 3 and 4, the aroma was more identical to natural, so the samples received 2.6 % higher scores. Samples from 3 experimental groups scored the most points in terms of taste. Compared with the control, the figure was higher by 3.6 %. Examining the juiciness of rabbit meat, it was found that samples obtained from carcasses 2 and 3 of the experimental group tended to increase this indicator. Summing up the data, it was found that the highest overall score was found in meat samples from 3 experimental groups. The difference with the control was within the trend and amounted to 2.4 %. Feeding rabbits vitamin and mineral supplements to rabbits helps to obtain good quality meat products with high culinary properties. No data were available to support the effect of vitamin and mineral supplements on compound feed on the synthesis of toxic substances in muscle tissue or the deterioration of rabbit meat. The biological value of muscle samples from rabbits of the 2-nd experimental group did not differ from the control data. The increase was only 0.11 %. It was found that the biological value of muscle tissue from animals of the 4th experimental group was greater than in the control. The difference was 2.6 % Meat obtained from rabbits of the 3rd experimental group, which were fed compound feed with a content of mineral-vitamin supplement "Tekro" 3.5 %, in biological value outweighed the control indicators. The difference was not significant and amounted to 4.1 %. The use of different doses of vitamin and mineral supplements in the feed of young rabbits did not lead to significant changes in the quality of raw hides and furs.