Quinoa Genotypes Research Articles

Genotype by environment interaction across water regimes in relation to cropping season response of quinoa (Chenopodium quinoa).

Genotype × environment (GxE) interaction effects are one of the major challenges in identifying cultivars with stable performance across agri-environments. In this study we analysed GE interactions to identify quinoa (Chenopodium quinoa) cultivars with high and stable yields under different soil moisture regimes, representing control conditions, waterlogging and drought. Waterlogging and drought treatments were artificially induced using normoxia, a combination of hypoxia-normoxia, and 10% PEG (Polyethylene glycol) under hydroponic growth conditions, respectively. Both waterlogging and drought conditions significantly reduced the plant height (PH), number of leaves (NoL) and number of branches (NoB), stem diameter (SD), leaf area (LA) and dry weight (DW) of quinoa genotypes. The genotype, water regime, and genotype by water regime effects all significantly affected the measured quinoa traits. Based on the additive main effects and multiplicative interaction (AMMI) model for DW, the genotypes G18, Puno, Q4, 2-Want, Puno, Real1 x Ruy937 and Titicaca were found to exhibit tolerance and were stable across water regimes. A second-stage evaluation was conducted to test genotype × environment interaction effects in crop production field trials, selecting two contrasting seasons based on soil moisture conditions involving a diverse set of genotypes (58 varieties in total). Our results demonstrate significant variations in both growth and yield among the quinoa genotypes across the cropping seasons. The GGE analysis for grain yield indicate that field conditions matched to G × E under hydroponic experimental conditions and the cultivars G18, Q1, Q4, NL-3, G28, 42-Test, Atlas and 59-ALC were classified within a range of high productivity. Our findings provide a basis for understanding the mechanisms of wide adaptation, while identifying germplasm that enhances the water stress tolerance of quinoa cultivars at early growth stages.

Differential growth, morphological characters, and yield of quinoa (Chenopodium quinoa Willd.) genotypes grown on salt degraded soil

AbstractQuinoa (Chenopodium quinoa Willd.), a high‐value halophytic crop, is a promising candidate to ensure food security in the scenario of increasing soil salinization due to climate change. In a 2‐year field study (during 2018–2019 and 2019–2020), 18 quinoa genotypes of different origins (Q‐4, Q‐6, Q‐9, Q‐7, Q11, Q‐15, Q‐22, Q‐24, Q‐27, Q‐45, Q‐50, Q‐51, Q‐52, Q‐76, Q‐81, Q82, Q‐124, and Q‐126) were grown at two different locations (salt‐affected and normal soil having electrical conductivity (EC) of 16.24 and 1.76 dS m−1, respectively). Morphological, physiological, and yield parameters were recorded to assess the impact of salinity on different genotypes of quinoa. All the tested genotypes performed better in normal soil (37% more yield) than salt‐affected soils. Under salt‐affected conditions, differential salt tolerance responses of quinoa genotypes were observed. Among tested genotypes, Q‐7 achieved the highest chlorophyll content index, biological mass (7905 kg ha−1), and seed yield (1916 kg ha−1) under salt‐affected conditions, it was followed by Q‐81. Salt stress caused up to 94% reduction of seed yield in the salt‐sensitive genotype (Q‐11), while the salt‐tolerant genotype (Q‐81) showed only 15% reduction in seed yield. Morphological characteristics of quinoa genotypes were differently influenced by salt stress. The salt‐tolerant accessions Q‐7 and Q‐81 exhibited similar morphological characteristics. Based on the findings of this study, salt‐tolerant quinoa genotypes can be successfully grown in salt‐degraded soils (with EC ≤ 16.24 dS m−1) in extreme winter seasons with arid climatic conditions.

Pomegranate-Quinoa-Based Agroforestry System: An Innovative Strategy to Alleviate Salinity Effects and Enhance Land Use Efficiency in Salt-Affected Semiarid Regions.

Salinity is a major problem, impeding soil productivity, agricultural sustainability, and food security, particularly in dry regions. This study integrates quinoa, a facultative halophyte, into a pomegranate-based agroforestry with saline irrigation in northeast Morocco. We aim to explore this agroforestry model's potential in mitigating salinity's effects on quinoa's agronomic and biochemical traits and evaluate the land equivalent ratio (LER). Field experiments in 2020 and 2021 used a randomized block design with three replicates, including monocropping and agroforestry systems, two salinity levels (1.12 and 10.5 dS m-1), four quinoa genotypes (Titicaca, Puno, ICBA-Q4, ICBA-Q5), and a pomegranate control. Salinity significantly decreased total dry matter (40.5%), root dry matter (50.7%), leaf dry matter (39.2%), and root-to-shoot ratio (7.7%). The impact was more severe in monoculture than in agroforestry, reducing dry matter (47.6% vs. 30.7%), grain yield (46.3% vs. 26.1%), water productivity (47.5% vs. 23.9%), and total sugar (19.2% vs. 5.6%). LER averaged 1.86 to 2.21, indicating 86-121% higher productivity in agroforestry. LER averaged 1.85 at 1.12 dS m-1 and 2.18 at 10.5 dS m-1, reaching 2.21 with pomegranate-ICBA-Q5 combination. Quinoa-pomegranate agroforestry emerges as an innovative strategy, leveraging quinoa's salt resistance and agroforestry's potential to mitigate salinity impacts while enhancing land use efficiency.

Phenotyping for Effects of Drought Levels in Quinoa Using Remote Sensing Tools

Drought is a principal limiting factor in the production of agricultural crops; however, quinoa possesses certain adaptive and tolerance factors that make it a potentially valuable crop under drought-stress conditions. Within this context, the objective of the present study was to evaluate morphological and physiological changes in ten quinoa genotypes under three irrigation treatments: normal irrigation, drought-stress followed by recovery irrigation, and terminal drought stress. The experiments were conducted at the UNSA Experimental Farm in Majes, Arequipa, Peru. A series of morphological, physiological, and remote measurements were taken, including plant height, dry biomass, leaf area, stomatal density, relative water content, selection indices, chlorophyll content via SPAD, multispectral imaging, and reflectance measurements via spectroradiometry. The results indicated that there were numerous changes under the conditions of terminal drought stress; the yield variables of total dry biomass, leaf area, and plant height were reduced by 69.86%, 62.69%, and 27.16%, respectively; however, under drought stress with recovery irrigation, these changes were less pronounced with a reduction of 21.10%, 27.43%, and 17.87%, respectively, indicating that some genotypes are adapted or tolerant of both water-limiting conditions (Accession 50, Salcedo INIA and Accession 49). Remote sensing tools such as drones and spectroradiometry generated reliable, rapid, and precise data for monitoring stress and phenotyping quinoa and the optimum timing for collecting these data and predicting yield impacts was from 79–89 days after sowing (NDRE and CREDG r Pearson 0.85).

Optimizing dyeing parameters for sustainable wool dyeing using quinoa plant components with antibacterial properties

This study focuses on the sustainable utilization of Quinoa plant components, particularly its leaves, as a waste agricultural material for natural dyeing applications. Two distinct Quinoa genotypes, Titicaca and Giza, were selected for their natural dyeing properties. UV-VIS absorption spectra of Quinoa colorant extracts provided insights into their chemical composition, revealing distinctive peaks indicative of betalains (400–450 nm), chlorophyll (600–650 nm), carotenoids (400–500 nm), and aromatic amino acids (250–280 nm). Wool samples dyed with different plant parts such as flowers, leaves, and stalks exhibited distinct yellow hue, with leaves demonstrating the highest color strength. Metal mordants such as iron (II) sulphate, copper sulphate, and tin (II) chloride influenced color outcomes, highlighting their role in tailoring final appearance. Fastness properties of dyed wool samples were evaluated, with leaves showing moderate staining resistance and good light fastness. Additionally, antibacterial properties of leaves of Giza Quinoa variety were assessed, showing promising activity against Staphylococcus aureus. The antibacterial properties of fabrics dyed with the leaf extract by mordanting method with different metal salt mordants, particularly Fe, Cr, and Cu, exhibited significant enhancement. These results highlight the multifaceted benefits of Quinoa plant components in sustainable natural dyeing and textile applications, emphasizing their potential for eco-friendly practices in the textile industry.

Nutritional potential, phytochemical analysis, and biological activities of quinoa (Chenopodium quinoa Willd.) seeds from arid zone culture

Quinoa, scientifically known as Chenopodium quinoa Willd., is well recognized for its exceptional nutritional composition and potential benefits for human health. This research aims to conduct a phytochemical analysis of nutraceutical properties and biologcal activities of seven different quinoa cultivars, namely, red, white, Amarilla marangani, kancolla, Giza 02, Amarilla sacaca, and black. The analysis of ground seeds revealed that Giza 02, white, and A. marangani had the most significant protein, fat, ash, and carbohydrates, respectively. Furthermore, the MeOH extracts of the various quinoa genotypes exhibited high levels of total phenolic, flavonoid, condensed tannin and reducing sugar contents in the seed extracts obtained using consecutive solvents. In addition, HPLC-DAD analysis revealed the presence of seven phenolic compounds, which may be classified into four families: phenolic acid, flavonoids, coumarins, and stilbenes. The IC50 values for the antioxidant activity ranged from 6.4 to 7.7 µg/mL. Furthermore, the variability in the antibacterial activity across different genotypes and gram-positive and gram-negative bacterial species was observed, and the efficacy of the CYHA-red extract against Micrococcus luteus was the highest. The findings of this study indicate that the incorporation of quinoa into the arid and Saharan cultivation system has the potential to enhance the nutritional and nutraceutical characteristics of the seeds.

Nutritional potential, phytochemical analysis, and biological activities of quinoa (Chenopodium quinoa Willd.) seeds from arid zone culture

Quinoa, scientifically known as Chenopodium quinoa Willd., is well recognized for its exceptional nutritional composition and potential benefits for human health. This research aims to conduct a phytochemical analysis of nutraceutical properties and biologcal activities of seven different quinoa cultivars, namely, red, white, Amarilla marangani, kancolla, Giza 02, Amarilla sacaca, and black. The analysis of ground seeds revealed that Giza 02, white, and A. marangani had the most significant protein, fat, ash, and carbohydrates, respectively. Furthermore, the MeOH extracts of the various quinoa genotypes exhibited high levels of total phenolic, flavonoid, condensed tannin and reducing sugar contents in the seed extracts obtained using consecutive solvents. In addition, HPLC-DAD analysis revealed the presence of seven phenolic compounds, which may be classified into four families: phenolic acid, flavonoids, coumarins, and stilbenes. The IC50 values for the antioxidant activity ranged from 6.4 to 7.7 µg/mL. Furthermore, the variability in the antibacterial activity across different genotypes and gram-positive and gram-negative bacterial species was observed, and the efficacy of the CYHA-red extract against Micrococcus luteus was the highest. The findings of this study indicate that the incorporation of quinoa into the arid and Saharan cultivation system has the potential to enhance the nutritional and nutraceutical characteristics of the seeds.

Heat Stress Resulting From Late Sowing Impairs Grain Yield and Quality of Quinoa Genotypes Facing Drought and Salt Stress Under Field Conditions

ABSTRACTClimate change is causing drastic reduction in crop yields around the globe due to increase in soil salinity, drought and heat stress. Quinoa (Chenopodium quinoa Willd) is regarded as a very significant food security crop considering the climate change scenario. Two quinoa genotypes (Puno and Titicaca) were cultivated on salt affected soil under drought stress with different sowing dates. Compared with early sowing, late sowing combined with salinity and drought stress caused drastic decline in plant growth and grain yield due to imposition of heat stress. Plant biomass and grain yield decreased by 26% and 39% in Puno, and by 34% and 49% in Titicaca under late sowing accompanied by salt and drought stress. Relative water contents and stomatal conductance of leaves declined in the same trend in both genotypes. Shoot Na+ concentration was the highest whereas K+ concentration was the lowest in both genotypes when drought and salt stress were combined under late sowing. Grain minerals (Ca, Mg, Fe, Zn, Cu, K, P, N and Mn) and dietary contents (protein, lipids, carbohydrates and fibre) were decreased more under the combination of salinity and drought for late sowing as compared to early sowing. When salinity and drought stress were combined under late sowing, the contents of H2O2 and TBARS were 1.9 and 2.2‐fold higher in Puno and 2.4 and 2.6‐fold higher in Titicaca, respectively. The oxidative stress was mitigated by enhanced activities of antioxidant enzymes (CAT, SOD and POD) more in Puno than Titicaca. Plant biomass and grain yield were higher in Puno with better grain quality than Titicaca. Hence, this genotype should be cultivated on salt affected soils facing drought and high temperatures.

Phenotypic Characterization and Yield Screening of Quinoa Germplasms in Diverse Low-Altitude Regions: A Preliminary Study

In this study, 300 quinoa accessions were systematically screened for adaptation to low-altitude areas using seventeen traits. The experiment was conducted in Taigu District, Jinzhong City, Shanxi Province, in 2021, at an altitude of 791 m. Out of the 300 genotypes, 107 were able to produce flowers and fruit, with yields ranging from 538 to 5132 kg ha−1 and with 1000-grain weights between 1.19 g and 2.37 g. These 107 quinoa genotypes were categorized into four groups based on grain yield: below 1500, 1500–2250, 2250–3000, and above 3000 kg ha−1. These groups consisted of 33, 33, 24, and 17 genotypes, respectively. This study found that the 1000-grain weight ranged from 1.19 g to 2.37 g, with an average of 1.72 g, 1.72 g, 1.83 g, and 1.92 g for the respective yield levels. Among the 107 genotypes, 25 had a 1000-grain weight exceeding 2 g, and 13 of these genotypes also had yields exceeding 2250 kg ha−1. The growth period of quinoa genotypes in the low-altitude area was approximately 138–142 days, with longer growth periods associated with higher yield levels. JQ-00084 is the only genotype with a yield > 3000 kg ha−1 and meets all the growth criteria, including a 1000-grain weight exceeding 2 g and a flowering-to-maturity period of over 80 days. Meanwhile, JQ-00315, JQ-00521, JQ-1029, and JQ-02405 fulfilled at least four criteria. These results highlight the importance of germplasm screening for low altitudes, and a large set of germplasms could be screened using the above criteria of phenology, growth, and yield traits.

The Performance of Agronomic and Quality Traits of Quinoa under Different Altitudes in Northwest of China

Quinoa is a resilient crop known for its adaptability to diverse environmental conditions. This study examined the agronomic performance and ecological adaptability of quinoa across four distinct altitudes in the northwestern regions of China. Six quinoa genotypes were assessed for agronomic traits, phenology and yield performances, and nutritional quality characteristics in Yining city, Nilka County, Tekes County and Zhaosu County under varied environmental conditions. Our findings demonstrate significant variations in all measured traits, including agronomic and nutritional quality traits, across the various altitudes and genotypes. In Yining city, a warmer and lower altitude area, genotypes exhibit superior traits for fodder production, including increased branches, longer panicles, robust stems, and high seed protein content. Genotypes Jinli 1 and Beijing 2 achieve yields exceeding 3 tons per hectare (t/ha), highlighting the region’s productivity. The Nilka and Tekes counties boasted the shortest quinoa crop cycles, with Jinli 1 yielding 4.05 t/ha seeds in Tekes County, exhibiting high protein and fat content alongside low saponins, making it a prime location for yield production. Zhaosu County, with its cooler climate and fertile chernozem soil, elicited high 1000 seed weight and more robust protein response than Nilka and Tekes counties, illustrating the impact of soil fertility on nutritional composition. Correlation analyses further elucidated that the plants characterized by shorter flowering times, crop cycles, compact inflorescence, and taller heights exhibited superior seed yields. Our study contributes significant insights into the ecological adaptability and nutritional dynamics of quinoa, with implications for sustainable crop production and food security in diverse agroecosystems.

Quantification of saponins by GC/MS in quinoa genotypes, Chenopodium quinoa Willd, from the southern highlands of Bolivia

Se realizó la cuantificación del contenido de saponinas en granos de siete genotipos de Quinua Real del Altiplano Sur de Bolivia: Quinua Real Blanca (QRB), Quinua Real Amarilla (QRA), Quinua Real Pandela (QRP), Quinua Real Toledo (QRT), Quinua Real Luki (QRL), Quinua Real Negra (QRN) y Quinua Real Rojo Pasankalla (QRRP), mediante la implementación de un método por Cromatografía de gases acoplada a espectrometría de masas (GC/MS). Ácido oleanólico, ácido serjánico, hederagenina y ácido fitolacagénico, de residuos de escarificado de quinua, fueron aislados y empleados como estándares. Se obtuvo extractos de sapogeninas de los 7 genotipos de Quinua Real, que fueron sililados a los trimetilsilil derivados y cuantificados por GC/MS. En todos los casos los valores de saponinas determinados resultaron altos, entre 0,8 y 1,4 %, confirmando que todas quinuas analizadas son quinuas amargas que deben ser sometidas a un proceso de beneficiado.

Quinoa growth and yield performance under salinity stress in arid West Texas

AbstractCrops such as quinoa (Chenopodium quinoa Willd.) that are both salinity and drought‐tolerant and with high seed value are needed to sustain agriculture in arid Far West Texas facing dual threat of freshwater scarcity and soil salinization. However, quinoa's growth and yield performance under arid conditions of Far West Texas has not been studied previously. This study evaluated growth and yield of a salt‐tolerant quinoa genotype under greenhouse conditions using a completely randomized experimental design with irrigation water salinity as the main factor having five different levels (freshwater, 5, 10, 15, and 20 dS m−1). Plant parameters (plant height, leaf SPAD, leaf tissue carbon, and nitrogen concentrations) and seed yield were measured for two growing seasons. Soil quality (salinity and sodicity) changes were also determined for the same time. Seed yields ranged between 747 and 6065 kg ha−1 across 2 years, indicating significant effects of water salinity. However, these yields were comparable to those reported in the literature. Increasing water salinity significantly affected all growth parameters with leaf C and N decreasing by an average of 20%, whereas reductions in plant height reached a high of 60% at 20 dS m−1. Similar reductions in leaf chlorophyll content were found with increasing water salinity. Soil salinity and sodicity significantly increased over time with irrigation water salinity. Importantly, we observed that quinoa has a much higher soil salinity threshold (∼12 dS m−1) above which yields declined rapidly. Higher salt tolerance threshold of quinoa makes it an alternative economically viable crop for the Trans‐Pecos Texas region.

Molecular and physiological responses of two quinoa genotypes to drought stress.

Quinoa is an important economic food crop. However, quinoa seedlings are susceptible to drought stress, and the molecular mechanism of drought tolerance remains unclear. In this study, we compared transcriptomic and physiological analyses of drought-tolerant (L1) and susceptible (HZ1) genotypes exposed to 20% PEG for 3 and 9days at seedling stage. Compared with HZ1, drought stress had less damage to photosynthetic system, and the contents of SOD, POD and CAT were higher and the contents of H2O2 and O2 -were lower in L1 leaves. Based on the RNA-seq method, we identified 2423, 11856, 1138 and 3903 (HZ1-C3-VS-T3, HZ1-C9-vs-T9, L1-C3-vs-T3 and L1-C9-vs-T9) annotated DEGs. Go enrichment was shown in terms of Biological Process: DEGs involved in biological processes such as metabolic process, cellular process, and single-organism process were most abundant in all four comparison treatments. In Molecular Function: the molecular functions of catalytic activity, binding and transporter activity have the most DEGs in all four processes. Cellular Component: membrane, membrane part, and cell have the most DEGs in each of the four processes. These DEGs include AP2/ERF, MYB, bHLH, b-ZIP, WRKY, HD-ZIP, NAC, C3h and MADS, which encode transcription factors. In addition, the MAPK pathway, starch and sucrose metabolism, phenylpropanoid biosynthesis and plant hormone signal transduction were significantly induced under drought stress, among them, G-hydrolases-66, G-hydrolases-81, G-hydrolases-78, Su-synthase-02, Su-synthase-04, Su-synthase-06, BRI1-20 and bHLH17 were all downregulated at two drought stress points in two genotypes, PP2C01, PP2C03, PP2C05-PP2C07, PP2C10, F-box01 and F-box02 were upregulated at two drought stress points in two genotypes. These results agree with the physiological responses and RNA-seq results. Collectively, these findings may lead to a better understanding of drought tolerance, and some of the important DEGs detected in this study could be targeted for future research. And our results will provide a comprehensive basis for the molecular network that mediates drought tolerance in quinoa seedlings and promote the breeding of drought-resistant quinoa varieties.

Screening for Salt Tolerance in Chenopodium quinoa Genotype Seedlings through Germination in a Hydroponic System

Quinoa (Chenopodium quinoa Willd.) is poised to be a global life changer with its ability to adapt to a wide range of abiotic stresses and as a highly nutritious and sustainable food source. A trial on screening of salt tolerance was conducted at the germination and seedling stages of 69 quinoa genotypes in different concentrations of NaCl 0 (CK), 100, 200, 300, 400, and 500 mM for 21 days in the MS/2 mixture. This results in 16 genotypes with >50% germination at 400 mM NaCl. These were reassessed in germination indices and relative growth. Results indicated that Chadmo had the highest germinability of 97% and 32.76% relative height among the 16 genotypes. Considering the germination indices, Chadmo had significantly different values (3.05±0.19 day-1) in mean germination time, coefficient of variation of the germination time (38.76±1.97%), the velocity of germination (0.23±0.01 day-1), the uncertainty of germination (0.54±0.08 bit), synchrony of germination (0.42±0.05 and Timson’s index (48.89) with significant differences (P<0.05) among the genotypes. Moreover, Chadmo had the highest membrane stability index (MSI) (60.03±11.84) at 400 mM NaCl and the least relative change between the CK and 400 mM NaCl with 30.87±2.01%. Assessing the stress inhibitory effect of the 16 genotypes, Chadmo had the least relative difference between the CK and 400 mM NaCl with shoot length of 34.34%, root length of 25.57%, fresh weight of 22.05%, dry weight of 3.62% and moisture content of 1.99% with Tukey analyses identifying significant differences (p<0.05). To select the salt-sensitive genotype, an assessment was done on five genotypes that exhibited the least germination at 200 mM NaCl. Kankolla had the least germinability with 12 and 4% at 100 and 200 mM NaCl, respectively. Considering all these parameters, Chadmo and Kankolla were selected as salt-tolerant and salt-sensitive for further analyses.

SALINITY TOLERANCE OF QUINOA (CHENOPODIUM QUINOA WILLD.) GENOTYPES TO ELEVATED NACL CONCENTRATIONS AT GERMINATION AND SEEDLING STAGES

Evaluating quinoa genotypes for salinity tolerance at germination and seedling stages is a prerequisite for plant breeders. Thus, the scrutiny of 19 quinoa genotypes at different salinity levels under controlled laboratory and greenhouse conditions occurred at the germination and seedling stages. This study aimed to identify the most tolerant genotypes to elevated salinity levels at germination and seedling stages and to determine the traits of a robust association with salinity tolerance using a factorial experiment based on a randomized complete block design in three replications. The four salinity solutions used were zero (control), 3000, 6000, and 9000 ppm NaCl. Increasing concentrations of NaCl caused a gradual and significant decrease for all studied traits except mean germination time, which significantly increased. At all salinity-stress levels (3000, 6000, and 9000 ppm NaCl), the studied 19 genotypes underwent classification based on their salinity tolerance index (STI) into three categories, i.e., tolerant, moderately tolerant, and sensitive. The four most salinitytolerant quinoa genotypes under all studied salinity-stress conditions were Rainbow-2, Ql3, RH, and KvlSRA2. The strongest correlations were between STI and each of seedling length, root length, seedling fresh weight, seedling vigor index I, and seedling vigor index II under 3000 ppm; germination percentage, speed germination index, seedling extent, root length, seedling fresh weight, and seedling vigor index II under 6000 ppm; and shoot length and seedling vigor index I under 9000 ppm salinity concentration level. Traits showing sturdy correlations with STI, high heritability estimates, high expected genetic advance, and wide phenotypic and genotypic variability were seedling dry weight, seedling fresh weight, seedling vigor index II, and speed germination index at all salinity stress concentrations; they are recommendable as selection criteria for salinity tolerance in quinoa at germination and seedling stages.

Non-targeted metabolomics analysis of metabolite changes in two quinoa genotypes under drought stress

BackgroundQuinoa is an important economic crop, drought is one of the key factors affecting quinoa yield. Clarifying the adaptation strategy of quinoa to drought is conducive to cultivating drought-tolerant varieties. At present, the study of quinoa on drought stress-related metabolism and the identification of related metabolites are still unknown. As a direct feature of biochemical functions, metabolites can reveal the biochemical pathways involved in drought response.ResultHere, we studied the physiological and metabolic responses of drought-tolerant genotype L1 and sensitive genotype HZ1. Under drought conditions, L1 had higher osmotic adjustment ability and stronger root activity than HZ1, and the relative water content of L1 was also higher than that of HZ1. In addition, the barrier-to- sea ratio of L1 is significantly higher than that of HZ1. Using untargeted metabolic analysis, a total of 523, 406, 301 and 272 differential metabolites were identified in L1 and HZ1 on day 3 and day 9 of drought stress. The key metabolites (amino acids, nucleotides, peptides, organic acids, lipids and carbohydrates) accumulated differently in quinoa leaves. and HZ1 had the most DEMs in Glycerophospholipid metabolism (ko00564) and ABC transporters (ko02010) pathways.ConclusionThese results provide a reference for characterizing the response mechanism of quinoa to drought and improving the drought tolerance of quinoa.

Evaluación agronómica de quinua intercalada con café a una altitud de 1.800 msnm

Quinoa (Chenopodium quinoa) exhibits great potential for adaptation to various agroecological conditions, making it a diversification option for different production systems. In this regard, the objective of this study was to evaluate and compare the agronomic characteristics of different progenies intercropped with coffee (Coffea arabica) cultivation under Popayan (Colombia) conditions, in order to determine their adaptation potential in coffee regions of Colombia. The research was conducted at the Experimental Farm "La Prosperidad", located in the municipality of Popayan, Colombia, at an altitude of 1,800 m. Ten genotypes were evaluated: five selected from BRS Syetetuba (Brazil), three from Colombia (San José, Aurora, and Blanca Dulce de Jericó), and two from Ecuador (Piartal and Tunkahuan). The planting was done between the coffee rows in the zoca stage, in an experimental area of 406 m2, following a completely randomized block design with four replications. Characteristics such as plant height, panicle size, central panicle perimeter, weight of 1,000 grains, grain yield, dry mass yield, harvest index, and phenological stages were evaluated. All quinoa materials showed an early cycle with a maximum value of 118 days, with ‘San José’, ‘Aurora’, ‘BCX1’, and ‘BCX4’ standing out with averages below 110 days. Grain yields ranged from 1,120 to 2,900 kg ha-1, with the genotype BCX6 standing out with 2,900 kg ha-1 and ‘Piartal’ with 2,883 kg ha-1. The weight of 1,000 grains averaged 2.62 g, and the harvest index was 27.96%. Meanwhile, the contribution of dry matter to the intercropped system was 7,799.50 kg ha-1. The genotypes showed adaptation potential in the intercropping system with coffee. A high variability of quinoa genotypes was observed, which is an interesting characteristic for specific selection processes in diversified production arrangements.

Evaluation of Different Genotypes of Quinoa (Chenopodium Quinoa Willd.) for Yield and Yield Contributing Parameters

The present investigation, "Evaluation of different genotypes of quinoa (Chenopodium quinoa Willd.) for yield and yield contributing parameters", was conducted to characterize the quinoa genotypes for different quantitative parameters. The experiment comprised thirteen genotypes, and accordingly, a trial was conducted. The experiment was conducted during Rabi, 2018, using Randomized Block Design with three replications under field conditions at All India Coordinated Research Network on Potential Crops, Department of Agricultural Botany, Mahatma Phule Krishi Vidyapeeth, Rahuri. Observations were recorded on five randomly selected plants in each treatment for branches per plant, inflorescence length (cm), inflorescence width (cm), seed yield/ plant (g), seed yield/plot (kg) and seed yield/ha (qtl). Among the genotypes, EC – 507749 (28.43 qtl/ha) recorded the significantly highest seed yield per hectare, which was followed by EC – 507747 (27.59 qtl/ha) & EC – 507743 (26.20 qtl/ha). The increase in seed yield per hectare was due to the increase in inflorescence length (27.06 cm), inflorescence width (22.63 cm) and branches per plant EC – 507749 (42.26).

Traits associated with higher productivity and resilience to drought-prone Mediterranean environments of coastal-lowland quinoa (Chenopodium quinoa Willd.)

Quinoa (Chenopodium quinoa Willd.) is an Andean crop that has been cultivated for over 7000 years in Andean South America. Among the five quinoa ecotypes identified, the coastal-lowland ecotype is distributed in central-southern Chile. Little is known about the phenotypic variability of this germplasm and its productive potential. In this work, we evaluated a panel of 99 self-pollinating genotypes of quinoa under rainfed and irrigated conditions in the Mediterranean area of central Chile. The aim was i) to evaluate the phenotypic variability of agronomic and leaf photosynthetic traits; ii) to assess trait responses to environmental conditions (rainfed and irrigated) and their heritability; and iii) to identify traits and genotypes associated with high yield potential and resilience in drought-prone environments. The selected genotypes were evaluated under rainfed (Hidango; 34° 06' S, 71°47' W) and irrigated (Santa Rosa; 36°31' S, 71°54' W) conditions, in two growing seasons (2016/17 and 2017/18). In the most favorable environment (Santa Rosa 2016), the shoot dry weight (shoot DW) and grain yield (GY) of the different genotypes, ranged from 655 and 2787 g m-2 (average 1440 g m-2) and from 45 and 452 g m-2 (average 244 g m-2), respectively. Under rainfed conditions (at Hidango), the shoot DW and GY were reduced by 75 % and 74 %, respectively, compared to Santa Rosa 2016, but still, some genotypes produced more than 100 g m-2, with < 70 mm of precipitation, evidencing a high drought tolerance. The harvest index (HI) and grain weight (TKW) were less affected by drought; compared to Santa Rosa 2016, the reduction was 8–18 % and 25–29 %, respectively, in 2016/17 and 2017/18. Days from sowing to flowering (DSF) and from flowering to maturity (DFM) varied among genotypes and were negatively correlated with GY and HI. Under rainfed conditions, the fluorescence parameters F0 and Fm were reduced by 30 % during flowering and by 50 % during grain-filling, compared to irrigated conditions. The HI and TKW showed the highest heritability (0.69) and were highly correlated with GY in all environments. This study showed that traits associated with higher productivity and resilience of quinoa genotypes in rainfed Mediterranean environments were early maturating and with high HI, and TKW. Finally, a set of genotypes presenting a high stress tolerance index (STI), and therefore higher tolerance to drought conditions, were identified and are candidates for further research focused on the selection of parentals for more advanced breeding stages.

Morphological characterization of introduced quinoa genotypes under Saudi Arabia conditions

Morphological characterization of introduced quinoa genotypes under Saudi Arabia conditions