Viscum album (VA) preparations have been used over the years to treat various diseases1. In homeopathy, VA mother tinctures and yours dynamized are described for cardiovascular diseases2. Nonetheless, the molecular mechanisms involved in the VA anti-hypertensive activity are poorly described. In this sense, the objective of this work was to investigate the cytotoxicity, the Na+/K+ ATPase activity and expression and also the generation of Reactive Oxygen Species (ROS) induced by winter and summer VA mother tinctures (TMVA) and their dynamized samples (6cH, 12cH and 30cH), in porcine proximal tubule renal cells (LLC-PK1). The cytotoxicity was performed by the MTT method after 24h of treatment with TMVA and yours respective dynamized samples (from Quercus petrea, Malus domestica, Ulmus carpinifolia, Pinus sylvestris and Abies alba), at 0.5% to 2.5% (V/V). Morphological alterations were evaluated by optic and transmission electronic microscopy. For the Na+/K+ ATPase and ROS assays, the cells were treated only with the concentration of 1.5% (V/V) of the samples. The MTT assay showed a dose-dependent cytotoxicity only for the summer Quercus robur TMVA (p<0.05). Considering the cytotoxic effect of this VAMT, it was not considered in the further experiments. Furthermore, no cellular alteration was detected in the LLC-PK1 cells after treatment with the selected TMVA and no significant effects were triggered neither by TMVA nor by the potentized samples (p>0.05) in the ROS generation (n=5). Only the TMVA of Abies alba summer reduced approximately 50% of the Na+/K+ ATPase activity (n=9). Also, the TMVA from Abies alba and Pinus sylvestris prepared with winter VA demonstrated capacity to reduce the expression of the enzyme Na+/K+ ATPase. The homeopathic potencies did not presented effect in the cytotoxicity and ROS generation and further analyses need to be performed to investigate its potential to reduce the Na+/K+ ATPase activity and expression.
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