AbstractThe atmospheric circulation around the Tibetan Plateau (TP) exhibits a substantial 10–20‐day quasi–biweekly oscillation (QBWO), profoundly impacting weather and climate locally and remotely. Understanding the factors influencing the generation of QBWO over the TP (QBWOTP) and its physical mechanism is crucial. This study has investigated the influence of multi–timescale and land–atmosphere interactions on the generation of the QBWOTP in surface potential vorticity (SPV), a valuable tool for characterizing the mechanical and thermal variabilities in mountain forcing, based on a 2014 case study. Results indicate that in the free atmosphere, the summer monsoon onset over the Bay of Bengal induces a northward shift in the westerly jet toward the TP, manifested as an increase in low‐frequency zonal winds. This shift facilitates the propagation of wave trains, leading to atmospheric quasi–biweekly potential temperature anomalies (θa) over the TP through a multi‐timescale interaction. Additionally, the TP's surface thermal forcing and arrival of wave trains trigger anomalous upward motion and increase cloud cover. The resultant decrease in net short‐wave radiations and increase in net long‐wave radiations contribute to variations in surface potential temperature (θs) over the TP. As θa and θs evolve, the difference between them enlarges, resulting in the generation of the SPV QBWOTP. Given the relationship between the QBWOTP and downstream rainfall, this study could provide novel insights into understanding and predicting downstream rainfall QBWO.