Here we present experimental results of compressional wave velocity (Vp) of muscovite-biotite gneiss from Higher Himalayan Crystallines (HHC) at the temperature up to 950°C and the pressure of 0.1–400 MPa. At 400 MPa, when the temperature is lower than 600°C, Vp decreases linearly with increasing temperature at the rate of (∂Vp/∂T)p = −4.43×10−4 km/s °C. In the temperature range of 600–800°C, Vp drops significantly and the signal is degraded gradually due to the dehydration of muscovite and α-quartz softening. When the temperature rises from 800°C to 875°C, Vp increases and the signals become clear again as a result of the temperature going through the β-quartz range. The experiments indicate that the duration has great influence on the experimental results when temperature is above the dehydration point of biotite. During the first 30 h at 950°C, the Vp decreases substantially from 5.9 to 5.4 km/s and the signal amplitude is attenuated by more than 80%. After the 30-h transition, the Vp and the amplitude of ultrasonic wave signals become steady. The decrease of Vp and attenuation of the signals at 950°C are associated with the breakdown reactions of biotite. The experiments suggest that the breakdown of muscovite and/or quartz softening can contribute to the low seismic wave velocity in thickened quartz-rich felsic-crust such as what is beneath southern Tibet. Additionally, α-β quartz transition generates a measurable high seismic velocity zone, which provides a possibility of precisely constraining the temperature in the upper-middle continental crust. Our study also demonstrates that duration is a key factor to obtain credible experimental results.