Quantum fluctuations of charge in the single electron box are investigated. The rounding of the Coulomb staircase caused by virtual electron tunneling is determined by perturbation theory up to the third order in the tunneling conductance and compared with precise Monte Carlo data computed with a new algorithm. The remarkable agreement for large conductance indicates that presently available experimental data on Coulomb charging effects in metallic nanostructures can be well explained by finite order perturbative results.