Quantitative Determination Of Organic Acids Research Articles

Rohova N. V. DEVELOPMENT OF TECHNOLOGY OF BUNDED JUICE ON THE BASIS OF FERTILIZED BIRCH JUICE

The purpose of the article is to develop the technology of blended juices based on fermented birch sap. Objectives of the article. The aim of the work was to develop biotechnology of production of fermented birch sap on the basis of preliminary selection of the most effective strains of microorganisms for fermentation, study of therapeutic and prophylactic properties of developed fermented birch sap by establishing the impact of a new product on metabolic processes and metabolic syndrome. determination of biological activity of the developed product against opportunistic pathogens. Research methodology. Different variants of blending ingredient ratios were developed and studied according to a set of organoleptic and physicochemical parameters. The degree of preservation of L-ascorbic acid after heat treatment was determined on model solutions. For this purpose, solutions of lactic acid in concentrations of 0.05 %, 0.1 %, 0.3 %, 0.5 % were prepared in distilled water, and ascorbic acid in concentrations of 0.005 % and 0.010 % was added to them. Organic acids were determined by high performance liquid chromatography in the reverse phase variant by photometric detection on a Waters instrument (USA). Quantitative determination of organic acids was performed according to standard solutions. Results. Freshly harvested birch juice is biologically unstable In the process of collection, it is contaminated with spontaneous microbiota and its shelf life before processing, although dependent on storage temperature, but is usually limited to 2 days. Therefore, it is important to prolong its shelf life by developing biotechnological methods to preserve the therapeutic and prophylactic properties of the original juice. Conclusions.So, modeling the loss of L-ascorbic acid during heat treatment allows us to draw an important conclusion for the technology, namely synthetic ascorbic acid should be added to blended juices based on fermented birch, that is after the fermentation process to establish an acidic environment. In terms of biochemical and microbiological composition, the most valuable can be considered fermented beverages – products of cultivation of mono or associated cultures of microorganisms. This is due to the ability of microorganisms to produce biologically active substances necessary for the normal functioning of the human body. The main difference and advantage of such beverages over conventional blending products is that bioactive substances are not introduced artificially, but are formed naturally during fermentation. If the source product is limited relative to a number of BAS or has a need to improve organoleptic characteristics, it is advisable to blend it with carriers of the desired properties.

Gas chromatography-mass spectrometric determination of organic acids in fruit juices by multiwalled carbon nanotube-based ion-pair dispersive solid-phase extraction and in situ butylation.

Fruit juices are naturally acidic, and the acidity is due to the formation of various organic acids formed in several metabolic processes. The content of acids varies due to various processing parameters during the preparation of fruit juices and their packaging for commercialization. Quantitative determination of organic acids provides the necessary information leading to changes occurred during processing. The organic acids were extracted by ion-pair dispersive solid-phase extraction by multiwalled carbon nanotubes and analyzed using gas chromatography-mass spectrometry (GC-MS) and in situ butylation. The developed analytical method was validated, and the obtained results showed a linearity in the range of 0.5-5000 μg/L levels of the analytes with limit of detection and quantification values of 2-10 and 5-20 μg/L, respectively. The inter- and intra-day reproducibilities are less than 15% with 80%-98% recoveries and less than 20% matrix effect. The developed method was used for the quantitative determination of organic acids present in fresh and packaged apple, grape, orange, and pomegranate juice samples. The content of organic acids was observed in the range of 0.26-3793 μg/L. Pimelic acid was not detected in any of the analyzed fruit juices. Fumaric acid (FA) was observed to be a major organic diacid present in the natural fruit juices. The results indicated that the processing of fruit juices for packaging decreases the content of organic acids in fruits. The developed GC-MS-based analytical method for the analysis of organic acids has good sensitivity and reproducibility for the quantitative determination of various organic acids in fruit juices. FA was observed to be the major carboxylic acid present in fruits. The processed juice samples possess the lowest concentration of organic acids, suggesting that processing has a significant effect on the concentration of organic acids in fruits.

Qualitative and Quantitative Determination of Organic Acids in Crude Herbal Drugs and Medicinal Herbal Preparations for Quality Control in Russian Federation by Modern Physicochemical Methods

Qualitative and Quantitative Determination of Organic Acids in Crude Herbal Drugs and Medicinal Herbal Preparations for Quality Control in Russian Federation by Modern Physicochemical Methods

Fast determination of lactic, succinic, malic, tartaric, shikimic, and citric acids in red Vranec wines by CZE-ESI-QTOF-MS.

A fast and simple method with CZE coupled to ESI/QTOF-MS was optimized and validated for quantitative determination of organic acids (lactic acid, succinic acid, malic acid, tartaric acid, shikimic acid, and citric acid) in red wines. The BGE was ammonium acetate and the separation of the analytes was performed in a polybrene-coated capillary in the presence of EOF. The sample preparation included dilution and filtration of the wine. The method showed satisfactory performance characteristics: good linearity for each organic acid, with correlation coefficients ranging from r2 =0.9902 (shikimic acid) to r2 =0.9990 (tartaric acid). The limit of quantification was between 0.0034mM (for shikimic acid) and 0.107mM (for citric acid), and the recovery data fell between 95.8% (malic acid) and 102.7% (lactic acid); the total run time was less than 4min. The RSD values for the interday repeatability and intraday reproducibility were between 3.44 and 9.50%, and between 1.75 and 8.29%, respectively. Seventeen Macedonian red Vranec wines were studied demonstrating a wide variation in the organic acids' concentration, which should be most probably due to the variation of the climate conditions in the vine areas.

LC with Evaporative Light-Scattering Detection for Quantitative Analysis of Organic Acids in Juices

In this work, a performing LC method with evaporative light-scattering detector was described for the analysis of tartaric, malic, ascorbic, citric and succinic acids in fruit juices. The method was optimized and validated in comparison with LC-UV, in terms of accuracy, LODs, LOQs and precision. The successive application to ACE, orange, pear, peach, mulberry and apple juices allowed separation and quantitative determination of organic acids in about 20 min just diluting and filtering the sample before LC determination. In the analysed juices, citric, malic and ascorbic acids were always present, and in all the samples, citric acid was the prevailing acid followed by malic acid except for peach and apple juices where an opposite trend was found. Tartaric and succinic acids were detected at lower concentrations and more rarely, except for peach juices, all containing tartaric acid, and ACE and orange juices, all containing succinic acid.

The development of quantitative determination method of organic acids in complex poly herbal extraction

Aim. The development of sensible, economical and expressive method of quantitative determination of organic acids in complex poly herbal extraction counted on izovaleric acid with the use of digital technologies.Materials and methods. Model complex poly herbal extraction of sedative action was chosen as a research object. Extraction is composed of these medical plants: Valeriana officinalis L., Crataégus, Melissa officinalis L., Hypericum, Mentha piperita L., Húmulus lúpulus, Viburnum.Based on chemical composition of plant components, we consider that main pharmacologically active compounds, which can be found in complex poly herbal extraction are: polyphenolic substances (flavonoids), which are contained in Crataégus, Viburnum, Hypericum, Mentha piperita L., Húmulus lúpulus; also organic acids, including izovaleric acid, which are contained in Valeriana officinalis L., Mentha piperita L., Melissa officinalis L., Viburnum; the aminoacid are contained in Valeriana officinalis L.For the determination of organic acids content in low concentration we applied instrumental method of analysis, namely conductometry titration which consisted in the dependences of water solution conductivity of complex poly herbal extraction on composition of organic acids.Result. The got analytical dependences, which describes tangent lines to the conductometry curve before and after the point of equivalence, allow to determine the volume of solution expended on titration and carry out procedure of quantitative determination of organic acids in the digital mode.Conclusion. The proposed method enables to determine the point of equivalence and carry out quantitative determination of organic acids counted on izovaleric acid with the use of digital technologies, that allows to computerize the method on the whole.

Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis.

This new method for qualitative and quantitative determination of organic acids, aldehydes, and ketones in biological samples is effective for use with urine, plasma, and amniotic fluid, and it requires no deproteinization. Isolation by batch-wise liquid partition chromatography on silicic acid follows formation of the O-(2,3,4,5,6-pentafluorobenzyl)oximes of oxoacids, aldehydes, and ketones. The total organic acid content of the sample provides a rapid screening test for metabolic abnormality. A wide-bore, bonded-phase capillary column was used for quantitative gas chromatographic-mass spectrometric analysis, followed by automated identification and quantification. Analytical recoveries were quantitative for a wide variety of metabolites. Gas-chromatographic retention indices, discriminating ions, and control ranges in amniotic fluid, plasma, and urine of adult subjects were determined for 61 biologically important compounds.

A GAS CHROMATOGRAPHIC PROCEDURE FOR THE DETERMINATION OF ORGANIC ACIDS AND REDUCING SUGARS IN FERMENTING CUCUMBER JUICE

ABSTRACTA simple gas chromatographic technique was developed for the quantitative determination of organic acids and reducing sugars in fermenting cucumber juice. Acids and sugars were simultaneously chromatographed as their trimethylsilyl derivatives with no prior fractionation reqdired. A 3% OV‐17 column provided resolution of acids, sugars and trimethylsilylimidazole, the silylating agent and solvent. Accurate quantitation of fructose and glucose consumption and lactic acid production were qbtained for cucumber juice samples fermented by Lactobacihs plantarum culture. The average and range of the average deviations of duplicates for all samples tested were ±0.015% and 0.001–0.045%, respectively. A study on the recovery of added acetic acid, lactic acid, glucose, and fructose was conducted with an average % redovery of 98.4% and a range of 95.2–101.1%.

Détermination quantitative des acides organiques dans les extraits végétaux : Amélioration de la technique d'analyse par chromatographie de partage sur colonne de gel de silice

Quantitative determination of organic acids in plant extracts. Improvement in the analysis by partition chromatography on a silica gel column The organic acids extracted from plant tissue are separated in two groups by passage over an anionic resin column, Amberlite IRA-400. The first group, eluted by means of a 0.9% aqueous solution of ammonium carbonate, includes the monocarboxylic acids pyrrolidonecarboxylic, glycolic, and glyceric; the second, eluted by means of a 6% aqueous solution of ammonium carbonate, comprises the dicarboxylic acids mesaconic, fumaric, succinic, malonic, oxalic, malic, and tartaric and the tricarboxylic acids cis- and trans-aconitic, citric and isocitric. The organic acids of each group are then automatically separated by partition chromatography on a silica gel column using as eluant for the first group a solvent containing chloroform and for the second a solvent containing chloroform and cyclohexane. The organic acids once eluted are determined by spectrophotometry in the presence of o-nitrophenol sodium salt.

Studies on Organic Acids in "Shottsuru" (Fish Sauce)

Quantitative determination of organic acids in Shottsuru (fish sauce) was done by means of a partition chromatography using silica gel and the changes in the amounts of organic acids were observed through the process of ripening.Eleven kinds of organic acids which were butyric, iso-butyric, propionic, acetic, oxal-acetic, pyruvic, fumaric, succinic, lactic and pyro-glutamic acids were confirmed in Shottsuru.Shottsuru prepared in a longer ripening period contained much more organic acids than that in a shorter ripening period.Iso-butyric acid and acetic acid were about 30% of total organic acids respectively.

Methylmalonic aciduria. A newly discovered inborn error.

Editorial Notes1 September 1968Methylmalonic Aciduria—A Newly Discovered Inborn ErrorLEWIS A. BARNESS, M.D., GRANT MORROW III, M.D.LEWIS A. BARNESS, M.D.Search for more papers by this author, GRANT MORROW III, M.D.Search for more papers by this authorAuthor, Article, and Disclosure Informationhttps://doi.org/10.7326/0003-4819-69-3-633_2 SectionsAboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinkedInRedditEmail ExcerptThe development of relatively simple methods during the last decade for the quantitative determination of organic acids in biological fluids threatens the practitioner with an inundation of organic acidurias not unlike that of the flood of aminoacidurias of the early 1950's. That some of the organic acidurias will occur in patients with aminoacidurias or other errors of metabolism will not be surprising, since the organic acids being measured are intermediates in the metabolic pathways of proteins, carbohydrates, and fats.Methylmalonic acid, originally identified in the urine of rats, is increased in the urine of patients with vitamin B12deficiency. It...

Quantitative Determination of Organic Acids in Feces

Quantitative Determination of Organic Acids in Feces