Quantitative Cytochemical Study Research Articles

A Quantitative Cytochemical Study of the "Polyploid" Liver Nuclei of the Frog Genus Pseudacris

A Quantitative Cytochemical Study of the "Polyploid" Liver Nuclei of the Frog Genus Pseudacris

Glycogen synthesis in normal, leukemic and polycythemic leukocytes. Preliminary report of a quantitative cytochemical study.

Glycogen synthesis in normal, leukemic and polycythemic leukocytes. Preliminary report of a quantitative cytochemical study.

A quantitative cytochemical study of chromosomal basic protein in static and proliferative cell populations

Measurements were carried out by microdensitometry of the amount of Feulgen and fast green dyes bound by interphase and mitotic figures in mouse testicular cells. It has been shown that the ratio of the two dyes in individual nuclei is constant in interstitial cells, dividing spermatogonia and spermatocytes in interphase.

A quantitative cytochemical and physiological study of the rat adrenal cortex in hypertrophy after unilateral adrenalectomy

A quantitative cytological and physiological study of the adrenal cortex cells during compensatory hypertrophy after unilateral adrenalectomy has been made. The weight increase of the gland is accompanied by an almost parallel increase in the number of cells. Therefore no marked variations in the average cell volume took place in the course of hypertrophy, except a slight increase in the second week. Instead, the nuclei of the cells showed notable modifications. Their volumes increased markedly in the first days after the operation and underwent later a rapid decrease, returning after about two weeks around the normal values. In the same period a parallel increase of the nuclear basic proteins (stained by Naphthol Yellow S) and of the total dry mass (measured by microinterferometry on aqueous and non aqueous nuclei) took place. Instead the amount of DNA/nucleus did not show any modifications during the whole experimental period. An early increase of the total cholesterol was observed, also on the basis of tissue weight. The maximal secretion of corticosterone was also increased within the first week of hypertrophy, both on the basis of body weight and of adrenal tissue weight, but decreased afterwards, showing that the hypertrophic adrenal tissue is physiologically less efficient than the normal one.

Some applications of interference microscopy in enzyme cytochemistry: With particular reference to alkaline phosphatase

The interference microscope can be applied to the quantitative cytochemical study of enzymes provided that some change in optical path difference is produced as a result of enzyme action. Microscopical interferometry has been applied to the study of alkaline phosphatase in various tissues, especially in the brush borders of renal tubules and intestinal epithelium. It has been possible to study the effects of hydrogen-ion and substrate concentrations, buffers, activators, and inhibitors. The properties of the enzyme in tissue sections are similar to those of the enzyme in homogenates and purified preparations.