Simple SummaryThis study aimed at investigating which pre-slaughter parameters determine variations in the quality of the loin derived from pigs intended for Italian PDO (Protected Designation of Origin) products. Data were collected on 44 commercial shipments of Italian heavy pigs. Meat quality parameters (pH, color lightness, drip loss, cooking loss, and shear force) identified two clusters: ‘Higher Quality’ (HQ) and ‘Lower Quality’ (LQ). The parameters which differed more widely between the two clusters were journey duration, ambient temperature, distance traveled and irregular behaviors (slipping, falling, and overlapping) at unloading. Among the pre slaughter parameters which negatively affect pork quality, consideration should be given to ambient temperatures above 22 °C, distance traveled above 26 km, travel duration between 38 and 66 min, and more than 5.9% of animals showing irregular behaviors at unloading. Journeys involving one or more of these risk factors may require additional attention in terms of animal welfare in order to obtain meat suitable for high-quality productions.This study focused on loin quality in Italian heavy pigs intended for the production of PDOs (Protected Designation of Origin) products, and investigated the pre-slaughter factors which negatively affect the quality of fresh meat. Data were collected on 44 shipments (loads) of pigs. Shipments were carried out under commercial conditions. Several pre-slaughter parameters were recorded within the entire process (on-farm, during transport, and at the slaughterhouse). On a subset of pigs (10 animals from every load, N = 440), serum cortisol and creatine kinase were measured and loin samples were analyzed for pH, instrumental color, drip loss, cooking loss, shear force, and sensory quality. Cluster analysis of the instrumentally-assessed meat quality parameters allowed the categorization of the shipments into two clusters: lower quality (LQ) and higher quality (HQ). Our results showed that the factors with significant differences between the two clusters were journey duration, ambient temperature, distance traveled, and irregular behaviors (slipping, falling, and overlapping) at unloading (all greater in LQ, p < 0.05). The pre-slaughter conditions associated with lower loin quality were ambient temperatures above 22 °C, distance traveled above 26 km, travel duration between 38–66 min, more than 5.9% of animals showing irregular behaviors at unloading.