Soil Quality Research Articles

Levels, distribution, and health risk assessment of phthalic acid esters in urban surface soils of Nagpur city, India.

Surface soil samples from residential, commercial, and industrial areas of Nagpur city, India, were collected to study the levels, distribution, and impact of land use patterns on phthalic acid ester (PAEs) contamination. The Σ6PAEs concentrations in soils from residential, commercial, and industrial areas ranged between 6,493 to 13,195µg/kg, 707 to 18,446µg/kg, and 1,882 to 5,004µg/kg with medians of 10,399, 6,199, and 3,401µg/kg, respectively. Bis-2-ethylhexyl phthalate (DEHP) and dimethyl phthalate (DMP) were the dominant PAEs in the urban soils. The concentrations of DEHP and DMP were significantly greater than those in Ontario's soil quality guidelines. Among the PAEs, benzyl-butyl phthalate (BzBP) was found at relatively high concentrations (1,238 and 9,171µg/kg) at two locations (i.e., S1 and S15). The chronic toxic risk (CTR) of PAEs was below the threshold, although the risk to children through ingestion and dermal exposure routes was greater than that to adults. The CR due to BzBP and DEHP were below the threshold level; however, the CR due to DMP was > 1 × 10-6 in residential areas. The cumulative CR of the six PAEs for adults (1.33-1.41 × 10-5) and children (8.08-8.89 × 10-6) surpassed the threshold level. This study revealed that PAEs in urban soils pose a risk to public health and require immediate risk reduction strategies.

Understanding how corn-yellow melilot intercropping combined with straw return increased stable organic carbon pool in Mollisols by reducing the application of chemical fertilizer

Long-term cultivation with persistent use of chemical fertilizers causes a rapid decline in organic matter content of Mollisols. But practicing intercropping along with straw return instead of the use of chemical fertilizer have the potential to reverse the decline and improve soil organic carbon (SOC) content. A field experiment with five treatments at two sites of Songnen Plain of Northeast China included the following treatments (1) corn monoculture with no chemical fertilizers and no straw return (c), (2) corn monoculture with chemical fertilizers and no straw return (c + F), (3) corn-yellow melilot intercropping and straw return combined with full application of chemical fertilizers (c&m + S + F), (4) corn-yellow melilot intercropping and straw return combined with half dose of chemical fertilizers (c&m + S + F/2), and (5) corn-yellow melilot intercropping and straw return without chemical fertilizers (c&m + S). The results showed that c&m + S + F, c + F, and c&m + S + F/2 decreased specific gravity and c&m + S + F increased water content. The c&m + S + F, c&m + S + F/2, and c&m + S improved SOC with 18.7%, 7.95%, and 8.17% (P < 0.05), respectively. The c&m + S + F and c&m + S + F/2 increased mineral-associated organic carbon, humus organic carbon, and heavy fraction organic carbon content. These findings provide a method for utilizing intercropping and straw return to replace chemical fertilizers to improve SOC and stable organic carbon content to ameliorate soil quality.

Adaptation of rhizobacterial and endophytic communities in Citrus Grandis Exocarpium to long-term organic and chemical fertilization.

Organic fertilizers (OF) are crucial for enhancing soil quality and fostering plant growth, offering a more eco-friendly and enduring solution compared to chemical fertilizers (CF). However, few studies have systematically analyzed the effects of OF/CF on root microbiome of medicinal plants, especially in combination with active ingredients. In this study, we investigated the composition and function of bacteria and fungi in the rhizosphere or within the root of traditional Chinese medicinal plants, Citri Grandis Exocarpium (Huajuhong), which were treated with OF or CF over 1, 3, and 5 years (starting from 2018). Additionally, we conducted metabolome analysis to evaluate the effects of different fertilizers on the medicinal properties of Huajuhong. The results indicated that extended fertilization could enhance the microbial population and function in plant roots. Notably, OF demonstrated a stronger influence on bacteria, whereas CF enhanced the cohesion of fungal networks and the number of fungal functional enzymes, and even potentially reduced the proliferation of harmful rhizosphere pathogens. By adopting distancebased redundancy analysis, we identified the key physicochemical characteristics that significantly influence the distribution of endophytes, particularly in the case of OF. In contrast, CF was found to exert a more pronounced impact on the composition of the rhizosphere microbiome. Although the application of OF resulted in a broader spectrum of compounds in Huajuhong peel, CF proved to be more efficacious in elevating the concentrations of flavonoids and polysaccharides in the fruit. Consequently, the effects of long-term application of OF or CF on medicinal plants is different in many ways. This research provides a guide for OF/CF selection from the perspective of soil microecology and aids us to critically assess and understand the effects of both fertilizers on the soil environment, and promotes sustainable development of organic agriculture.

Potential to Ensure Safe Production of Water Spinach in Heavy Metals-Contaminated Soil by Substituting Chemical Fertilizer with Organic Fertilizer

Organic fertilizers are widely used to improve soil quality. However, their potential for ensuring the safe production of vegetables in soils with varying levels of heavy metals pollution remains inadequately explored. Here, we conducted a pot experiment to investigate the effects of substituting chemical fertilizers with organic fertilizer on the HMs accumulation in water spinach by simulating soils with different levels of HMs pollution. The results showed that the organic fertilizer significantly increased the soil pH, cation exchange capacity (CEC), and organic matter (OM). Furthermore, it led to a reduction in the soil DTPA–Cd and DTPA–Pb levels by 3.3–20.6% and 22.4–47.3%, respectively, whereas the DTPA–As levels increased by 0.07–7.7 times. The organic fertilizer effectively reduced the Cd and Pb content in water spinach below the safety limits when the added Cd content in the soil was less than 2 mg/kg and the Pb content was equal to or less than 90 mg/kg. However, its efficacy in reducing As accumulation in water spinach was limited, emphasizing the need for caution when using organic fertilizers in As-contaminated soils. Our results provide valuable insights for the scientific and precise utilization of organic fertilizers, thereby contributing to the safe production of vegetables.

The delineation of management zones using soil quality indices for the cultivation of irrigated rice (Oryza sativa L.) in Huila, Colombia

Conventional intensive farming systems can result in degraded soil. It is therefore important to monitor this effect periodically by delineating management zones (MZ) based on soil quality indices (SQI) in order to maintain and improve the soil characteristics in a precision farming environment and obtain homogeneous rice yields. The aim of this study was to determine and spatialise SQI and delineate MZ for cultivating flooded rice in an area of Fluvisols in Huila, Colombia. Forty-one georeferenced soil samples were collected from the 0 to 20 cm layer, and the physical, chemical and biological attributes of the soil were analysed to calculate the Integrated Quality Index (IQI) and the Nemoro Quality Index (NQI) using linear scoring functions. Geostatistical tools were then used to fit semivariogram models of the SQI, and interpolated using ordinary kriging to map the MZ using the QGIS software. The IQI and NQI showed a moderate spatial correlation, which allowed three distinct MZ to be identified and delineated. Attributes, such as bulk density (Bd), total porosity (TP), soil respiration (SR), available water (AW) and soil organic matter (SOM) were significant and can be used as a guide by farmers for restoring the quality of the soil in rice production. The method proved to be effective, and provided an information base to be used in the local management of areas of rice cultivation in the study region.

Pedagogical technologies for teaching environmental engineering in the online learning system of higher education institution

The article outlines the pedagogical technologies for teaching environmental engineering in the online learning system of higher education institutions. There are proposed ways of developing educational technologies for teaching environmental engineering: active and collaborative learning, individualisation of training, use of video and audio materials, using electronic tools for evaluation of educational results, development of digital literacy, implementation of interactive online courses and professional communication and mentoring. There are presented the use of these technologies in the different aspects of environmental engineering, such as analysing water quality parameters, soil quality assessment, studying waste management practices, and studying the integration of renewable energy into the practice through virtual labs, online tests, video lectures, and collaborative projects. The proposed pedagogical technologies for teaching environmental engineering in the online learning system of higher education institutions were tested in the educational process. The Student's t-test was used for statistical calculation of the obtained experimental data. At the end of the experiment, there is a statistically significant difference between the mean values of the experimental and control groups. It can be claimed that the use of pedagogical technologies for teaching environmental engineering in the online learning system of higher education institutions is perspective.

Integration of indigenous and formal knowledge in the assessment of soil quality performance assessment using multiple factor analysis in Alborz central mountains

Despite the data gap between researchers and farmers in soil fertility assessment, there are similarities and coincidences between the achievements of scientific versus local assessments. Farmers’ land management practices are a function of perceived soil fertility assessment in day-to-day management decisions. Despite the efforts made, studies have yielded different results when comparing soil fertility assessments in terms of indigenous and scientific knowledge, thus calling for closer assessments using extensive creative and systematic approaches. The current study uses Multiple Factor Analysis (MFA) to propose efficiency indicators based on the weighting of familiar, organizational, or productive variables in groups evaluating indigenous, scientific, and data variability. The end goal is to help identify the decision-makers’ training needs and decide on future institutional endeavors for developing productive skills and adopting better measures to evaluate soil fertility. By combining indigenous knowledge with innovations and improving the relationship between scientists and indigenous peoples and also promoting collaboration, thinking, and knowledge acquisition of farmers on how to maintain soil fertility and health, we hope to move towards sustainable agriculture as an institutional reform.

Effect of Soil Amendments on the Aggregate Stability, Biological Activity, and Yield of Canola (Brassica napus L.) in Degraded Soils

ABSTRACT Recycling organic waste and water treatment residues in nanoform is a promising technology in various farming systems, and its effect on aggregate stability, biological activity, and canola production in degraded soils is largely unknown. Hence, we evaluated the effect of nano-biochar (nB) and nano-water treatment residues (nWTR) at levels 50, 100, and 250 mg kg−1 as compared to soil without amendments on the aggregate stability, biological activity, and canola yield in degraded soil. The results showed that the structure coefficient (SC) values are higher when nB and nWTR are added at 100 mg kg−1 soil than at levels 50 and 250 mg kg−1. In our study, the high aggregate stability in the soil treated with nB and nWTR is likely due to their higher content of nutrients, organic carbon, clay, and CEC. The seed weight of the canola plant increased with increasing nB by more than 64%, while it decreased with increasing nWTR. Microbial biomass carbon (MBC) and the activity of dehydrogenase (DHA) and catalase (CLA) increased significantly in the treated pots with the addition of nB and nWTR at various rates. The results of the pot experiment recommended that the applications of nWTR and nB could be used as a promising strategy to improve soil quality and crop yield while also increasing the recycled efficiency of WTR and rice straw.

Evaluating soil quality and carbon storage in Western Ghats Forests, Karnataka, India, for sustainable forest management.

Monitoring soil quality index (SQI) and soil organic carbon (SOC) stock status of the Western Ghats (WG) forests in India is crucial for providing vital ecosystem services alongside sustainable forest management practices. However, comprehensive profile data on SQI and SOC stock across different forest types under WG forests are limited. The study evaluated SQI and SOC stock under three forest types, i.e. tropical wet evergreen (TWE), tropical semi-evergreen (TSE), and tropical moist deciduous (TMD) across WG in Karnataka. SQI was assessed using principal component analysis with two indexing approaches and scoring methodologies, with weightage indexing through nonlinear scoring functions (NLSF) showing superiority over other methodologies. TMD forests exhibited the highest SQI, followed by TWE and TSE, while the lowest was observed in Rippon Pet RF (0.36 surface, 0.28 control section), primarily due to limitations in organic carbon and clay content. SOC stock mirrored SQI trends (TMD > TWE > TSE), with the highest values in Kollegal RF (339.3 MG ha-1) and lowest in Rippon Pet RF (102.5 MG ha-1). Although SOC and SQI were established to be ideal indicators for dynamic ecosystem services (ESs), high OC content in surface soils of Poomale NF induces pedogenic acidification and Al toxicities, indicating potential forest soil degradation. Significant correlation with control section SQI and SOC (p < 0.05) emphasises monitoring subsurface soil status to identify soil degradation, sustainable forestry practices, and complex ESs in forest systems.

An overview of soil and plant assessment for predicting site quality and recovery strategies of one the largest tailings dam failures worldwide.

Brazil's Fundão dam collapse is one of the world's largest disasters of tailing dam failures. Previous research has evaluated toxic metals and non-metals (Cd, Cr, Ni, Pb, As, Hg) in the same soil samples used in this study, and results have indicated that only Fe and Mn concentrations increased above the original baseline (Melo et al., 2023). Consequently, the present study's focus has shifted towards assessing and integrating changes in soil quality regarding chemical fertility and morphological, physical, and mineralogical attributes in the floodplains post-dam collapse. Soil samples from 0 to 0.2 and 0.2-0.4m depths, and samples of Urochloa sp. were collected along ten transects, spanning 100km perpendicular to the Doce River channel. This sampling strategy targeted specific landscape positions including areas affected by deposited iron tailings (DIT), soil tailing mixture (STM), and control soil (CS) devoid of iron tailing interference. Results showed no discernible alterations in Ca, Mg, K, and P concentrations in Urochloa sp., and the most severe negative impacts observed regarded the replacement of kaolinitic pre-disaster matrix for hematitic matrix, reduction in organic carbon, and the prevalence of sand and silt particles. These factors collectively contributed to triggering: (i) decrease in chemical fertility and cation exchange capacity and (ii) significant decline in physical quality, evidenced by increased density and reduced total porosity and macroporosity. Addressing these adverse effects would require the augment of organic matter levels and offset the dominance of the hematitic matrix in the DIT. Furthermore, it is imperative to decompact the DIT by mechanized or plant cultivation means.

Effect of slope on water run-off and soil vulnerability in an unglaciated sub-watershed: a case study of conservation practice siting

For maximization of soil and water quality benefits, the right agricultural conservation practices (CPs) should be practiced for the right place. The Agricultural Conservation Planning Framework (ACPF) is a tool that helps decision makers achieve precise conservation within a target watershed using rapid, low-cost Geographic Information System-based tools. ACPF takes the information on landscape characteristics and combines it with user input to identify locations susceptible to soil loss and water run-off and suggest potential CP locations. Suggested CPs may further be analyzed for urgency, installation cost, and effectiveness. In this study, ACPF was applied to analyze soil and water vulnerability within a small unglaciated sub-watershed in southwest Wisconsin and identify potential CP practices and recommended placement sites. To promote environmental sustainability in the agriculture sector, land managers are tasked with implementing CPs, but often lack adequate information on the most effective practice and placement strategy. Field-scale siting of CPs was achieved using high-resolution terrain derivatives and accurate simulation of the stream network. Slope disaggregation among soil loss vulnerability categories was also conducted. Greatest sources of vulnerability were associated with slope and erodibility factors, with average slope within cropped fields being 6% and erodibility being as high as 43%. Fields classified as critical for conservation had a mean slope of 7.4%. Suggested contour buffer strip (CBS) placement per field varied with slope, with steeper fields having denser placement. The results suggested that 3% of cropland could be removed from production if the recommended placement of CBS was implemented. A further 0.2% of the sub-watershed area would go to grassed waterways. The results support implementation of effective CPs based on local conditions and act as a tool to help reveal if existing practices in the sub-watershed are of a size and extent comparable to the predicted potential.

The effect of aeration and irrigation on the improvement of soil environment and yield in dryland maize.

The aim of this study was to examine the effect of long-term aerated seepage irrigation technology on soil fertility changes and maize yield under continuous maize cropping system in red loam soil, and to explain the mechanism of maize yield increase under this technology, which can provide theoretical basis for crop quality improvement and yield increase under aerated irrigation (AI) technology. Therefore, this research was conducted for four field seasons in 2020-2023 at the National Soil Quality Observation Experimental Station, Zhanjiang, China. Soil aeration, soil fertility, root growth, physiological traits, and yield indicators were evaluated by conventional underground drip irrigation (CK) and AI. Our results showed that AI treatment significantly improved soil aeration and soil fertility. Increases in soil oxygen content, soil respiration rate, soil bacterial biomass, and soil urease activity were observed, corresponding to increases from 3.08% to 21.34%, 1.90% to 24.71%, 26.37% to 0.09%, and 12.35% to 100.96%, respectively. The effect of AI on maize indicators increased year by year. Based on improvements in soil aeration and fertility, root length, root surface area, and root dry weight under AI treatment were enhanced by 15.56% to 53.79%, 30.13% to 62.31%, and 19.23% to 35.64% (p < 0.05) compared to the CK group. In addition, maize agronomic traits and physiological characteristics showed improved performance; in particular, over 1.16% to 14.42% increases were identified in maize yield by AI treatment. Further analysis using a structural equation model (SEM) demonstrated that the AI technology significantly promotes the improvement of root indicators by enhancing soil aeration and soil fertility. As a result, maize yield could be increased significantly and indirectly.

Associations of Arbuscular Mycorrhizal Fungi (AMF) for Enhancements in Soil Fertility and Promotion of Plant Growth: A Review

Arbuscular Mycorrhizal Fungi are used for soil fertility enhancements and stimulating plant growth in which they association with other organisms like terrestrial plants. Mycorrhizas create an association between fungi and the roots of plants. Therefore, the review was made to point out important fungal species involved in fungal plant interaction and their major roles in agriculture as well as ecosystem. 80% of plants form associations with mycorrhizal fungi. The fungal are used to use their different organs like chain, arbuscular, vesicle, supportive cells and spore to interact with the other plant/ plat’s organ. The mycorrhizal fungi can be categorized into two principal classifications based on their anatomical interactions with the roots of host plants. Arbuscular Mycorrhizal and Ectomycorrhizal fungi utilize two distinct strategies for nutrient acquisition. The main categories of vesicular arbuscular mycorrhizal associations are linear or coiling and of ectomycorrhizal associations are epidermal or cortical. The rhizospheric and endophytic microbes promote plant growth as inoculated with crop. AM fungi as an obligate symbiont share a distinct feature called arbuscules as a site of nutrient exchanges between host and fungi. Arbuscules developed between cell wall and plasma membrane of root cortical cells and differentiated from plant plasma membrane by periarbuscular membrane. Arbuscular mycorrhizal fungi (AMF) play an indispensable role in augmenting plant nutrient acquisition, enhancing plant resilience and tolerance to various environmental stresses, improving soil fertility and structure, and providing numerous beneficial effects. AMF engage in interactions with other soil microorganisms, such as plant growth-promoting rhizobacteria, resulting in a synergistic effect that promotes plant growth and offers protection against pathogens associated with Rhizobia. Both AMF and Rhizobia utilize the same signaling pathways, which facilitate their association with host plants and enable nitrogen fixation within the soil ecosystem. A positive relationship has been established between AMF colonization and the diversity of soil microbial communities. Nitrogen-fixing rhizobia, mycorrhizal fungi, and root nodule symbioses typically exhibit synergistic interactions concerning infection rates and their effects on mineral nutrition and plant growth, thereby significantly enhancing the status of soil fertility, particularly with respect to soil quality characteristics.

Knowledge Atlas of Cultivated Land Quality Evaluation Based on Web of Science Since the 21st Century (2000–2023)

Cultivated land is the most important natural resource for human survival and development. The quality of cultivated land is closely related to grain output, and whether it can guarantee stable food supply is directly related to national food security. Cultivated land quality evaluation is an effective tool for understanding and mastering cultivated land quality. However, few studies have applied bibliometrics to quantitatively and systematically analyze this field. We used VOSviewer 1.6.19 and CiteSpace 6.3.1 software to visually analyze and construct 2478 documents related to cultivated land quality evaluation retrieved from the Web of Science core collection database from 2000 to 2023. Results show that cultivated land quality evaluation is still a popular research field. The collaboration ability among authors is weak and the distribution of institutions and countries publishing in this field is very uneven. In addition, the relevant research has been published in a variety of journals such as agriculture, environment, ecology, and computer technology. The research content is becoming more and more interdisciplinary. Keywords such as “Soil quality”, “Swat”, “Remote sensing”, “Heavy metals” and “Ecosystem services” have become hot topics in this field. In the future, it is necessary to further deepen the connotation of cultivated land quality, develop a long time series dynamic model of cultivated land quality evaluation and monitoring, and enhance the transformation of research results into practical applications.

Fertilization shapes microbial life strategies, carbon and nitrogen metabolic functions in Camellia oleifera soil

Mineral, organic and microbial fertilizers are crucial for maintaining and improving soil quality, ecosystem functions, and fruit yield in Camellia oleifera plantations. However, how the application of these fertilizers shapes the life strategies and functions of soil microbial communities is unclear. Here, we conducted a one-year field experiment with three types of fertilizers: mineral (NPK), manure (Man), and microbial (MicrF), and analyzed soil properties, bacterial and fungal communities to assess microbial life strategies, functional traits and their determinants. The application of MicrF strongly increased the diversity of both soil bacterial (by 6.4%) and fungal communities (by 23%). Organic matter inputs from Man and MicrF had greater effects on the life strategies of bacteria than fungi: the dominant r-strategy bacteria (Proteobacteria, Bacteroidetes, and Actinobacteria) increased with Man and MicrF, but K-strategists (Acidobacteria) decreased. Conversely, the abundance of r-strategy fungi (Ascomycota) decreased, but that of K-fungi (Basidiomycota) increased. Predictions of the functions indicated that microbial fertilization accelerated the bacterial carbohydrates, carbon and nitrogen metabolism, while also increasing the prevalence of wood saprotrophic fungi. The changes in the taxonomic and functional characteristics of the microbial communities induced co-metabolism priming effects, which were mainly regulated by soil organic carbon, available phosphorus, ammonium nitrogen contents, and carbon-nitrogen ratio. The application of MicrF is an effective approach to increase the diversity and multifunctionality of soil microbial communities in Camellia oleifera plantations, including organic matter decomposition, carbon and nitrogen metabolism. These findings provide valuable insights into the fertilizer regimes based on microbial ecological strategies and functional profiles in Camellia oleifera plantations.

Enhancing soybean yield stability and soil health through long-term mulching strategies: Insights from a 13-year study

Sustainable agriculture systems incorporate important stabilizing mechanisms, such as mulching, for increasing yield and improving soil health. However, the synergistic effects of different long-term mulching practices on soybean yield stability and soil health remain unexplored. In this study, we conducted a 13-year long-term investigation to evaluate the impacts of various mulching methods—no mulching (CK), straw mulching (SM), plastic mulching (PM), and ridged and plastic mulching (RM)—over a 13-year period on soil nutrients, and soybean yield, stability, and sustainability. Our findings revealed that SM, PM, and RM treatments significantly increased the average yields by 28.02 %, 20.49 %, and 51.56 %, respectively. Moreover, SM and RM treatments significantly enhanced yield stability (SM +107.90 %, RM +98.82 %) and sustainability (SM +47.85 %, RM +37.14 %). Additionally, compared to CK, the SM treatment significantly increased the average soil organic carbon (SOC) and total nitrogen (STN) content by 16.78 % and 16.23 %, respectively. Meanwhile, mulching practices also improved soil reactive carbon and nitrogen pools. Compared to CK, plastic mulch reduced microbial biomass carbon (MBC) content (PM −8.85 %, RM −0.73 %) and soil ammonium nitrogen (AN) content (PM −8.19 %, RM −1.20 %), while increasing microbial biomass nitrogen (MBN) content (PM +8.73 %, RM +9.47 %). The SM treatment increased MBC, AN, and MBN contents by 0.24 %, 7.23 %, and 8.94 %, respectively. Additionally, SM and RM treatments significantly increased β-1,4-glucosidase (BG) activity (SM +98.74 %, RM +128.25 %) and decreased and β-1,4- n -acetamido-glucosidase (NAG) + 1-leucine aminopeptidase (LAP) (NAG + LAP) activity (SM −28.74 %, RM −25.33 %) compared to CK. Furthermore, SM, PM, and RM treatments significantly increased the Chao1 index by 35.30 %, 68.08 %, and 52.23 %, respectively, compared to CK. Finally, results of the Mantel test and random forest model indicated that the increases in yield and stability were due to improved soil temperature (ST), active carbon and nitrogen pools, enzyme activity, and diazotrophic bacterial diversity. In summary, our findings suggest that ridged and plastic mulching enhances soil nutrient effectiveness by maintaining soil moisture and regulating diazotrophic bacterial community structure, thereby increasing soybean yields. Conversely, straw mulching continuously supplies nutrients to the soil, enhancing soil quality and diazotrophic bacterial community structure, thus improving yield stability. Over all, our findings provides new insights into global long-term agricultural sustainability.

The long-term effect of biochar application to Vitis vinifera L. reduces fibrous and pioneer root production and increases their turnover rate in the upper soil layers.

Fibrous and pioneer roots are essential in the uptake and transport of water and nutrients from the soil. Their dynamic may be influenced by the changing of soil physicochemical properties due to the addition of biochar, which, in turn, has been shown to improve plant growth and productivity in the short term. However, the long-term effects of biochar application on root dynamics are still widely unknown. In this study, we aimed to investigate the long-term effects of biochar application on grapevine fibrous and pioneer root dynamics and morphological traits in relation to soil characteristics. To this aim, grapevine plants amended in 2009 and 2010 respectively with one and two doses of biochar, were analyzed in their fibrous and pioneer root production and turnover rate, standing biomass, length, and specific root length, over two growing seasons. Our findings demonstrate that in the long term, biochar application significantly increased soil pH, nutrient availability, and water-holding capacity causing a decrease in the production of fibrous and pioneer roots which is reflected in a reduction of the root web characterized though by a higher turnover rate. Furthermore, we observed that these root morpho-dynamical changes were of higher magnitude in the upper soil layers (0-20 cm) and, at least in the long term, with no significant difference between the two doses. These results suggest that in the long term, biochar can be a powerful tool for improving soil quality, which in turn lowers carbon-cost investment toward the root production and maintenance of a reduced root web that might be directed into grapevine growth and productivity. Such effects shed some light on the root plastic and functional adaptation to modified soil conditions facilitated by the long-term application of biochar, which can be used for implementing adaptive agricultural practices to face the current climate change in a frame of sustainable agricultural policies.

Soil metabolic disturbance drives replant disease (intraspecific negative plant–soil feedback): Insights from an experiment examining soil impacts up to 20 years after a ginseng crop

Replant diseases (RDs), intraspecificnegativeplant–soilfeedback, often stem from nutrient deficiency, allelopathy, or pathogen accumulation. However, the RDs of certain crops are long-lasting and their causes remain unknown. We examined Panax quinquefolius RD in a space-for-time soil sequence representing crop rotation restoration over 1, 10, and 20 years using multiomics and bioassays. Compared with the soils with no ginseng cultivation history, we found 110 significant potential factors related to RD, surprisingly, 53 of which remained unrestored after 20 years. Soil pH and the levels of organic nutrients (amino acids, carbohydrates, and alditols), allelopathic-promoting metabolites (phenolic acids, amines, pyridines, etc.), and beneficial bacteria (Sphingomonas, Burkholderia-Caballeronia-Paraburkholderia, and Terrabacter) and fungi (Acremonium, Penicillium, and Naganishia) decreased, while the levels of allelochemicals (pyruvic and fatty acids) increased. The expression of all metabolic pathways was significantly down-regulated, with the exception of the up-regulated fatty acid-related metabolic pathways. We confirmed that decreased organic nutrient levels and increased levels of allelochemicals impaired P. quinquefolius growth. Soil metabolic factors rather than microbial factors were dominant by network analysis. In conclusion, we found that the overall changes in nutrient levels and metabolic and microbial factors contributed to short-term RD (1–10 year) persistence, whereas long-term RD (after 20 years) primarily resulted from disordered soil metabolite levels and pathways. This research will help deepen our understanding of the relationship between RD and potential changes in the factors influencing RD that are caused by soil legacy effects of valuable plants and provide theoretical guidance for effective soil quality improvement.

Investigating the Synergistic Effect of Tillage System and Manure Application Rates on Selected Properties of Two Soil Types in Limpopo Province, South Africa

Sustainable agricultural practices are needed to find a solution to the problem of soil erosion and decreased soil quality. A study was conducted during the 2021/2022 and 2022/2023 cropping seasons to evaluate the synergistic effect of the tillage system (TS) and manure rates (MR) on selected soil properties at the University of Limpopo Experimental Farm (Syferkuil) and University of Venda Experimental Farm (UNIVEN). The experiment had a split plot design with three replications. The main plots used conventional (CON) and in-field rainwater harvesting (IRWH) tillage systems, while subplots used poultry and cattle manure at rates of 0, 20, and 35 t ha−1. Bulk density (BD), aggregate stability (AS), pH, total N, organic carbon (OC), available P, and exchangeable cations (Ca, Mg, and K) were determined. IRWH significantly increased AS in the 0–20 cm soil layer at Syferkuil. TS × MR interaction significantly influenced AS and total N in the 20–40 cm soil layer during the 2022/2023 season at Syferkuil. IRWH significantly increased Mg content in the 2021/2022 season and total N, OC, and Mg content in the 2022/2023 season at Syferkuil over CON. At UNIVEN, CON significantly increased total N, whereas IRWH increased available P in the 2022/2023 season. MR significantly increased AS, exchangeable Ca, Mg, and K at both sites. At Syferkuil, MR significantly increased total N, OC, and available P during both seasons, whereas at UNIVEN the significant increase was observed on OC and available P during both seasons and total N in the 2021/2022 season. It was found that IRWH and poultry manure (35 t ha−1) improved most soil properties at both sites; however, this study recommends long-term experiments to investigate the combined effect of IRWH and manure rate on soil properties to validate the findings observed in this study.

Rhizophagus intraradices symbiosis with Amaranthus hypochondriacus improves rhizosphere soil pH and nutrient status in sodic soil

As a forage crop belonging to the Amaranthaceae family (often considered as non-mycorrhizal plants), Amaranthus hypochondriacus can grow in sodic soil. It is worth exploring whether arbuscular mycorrhizal fungi (AMF) can successfully form a symbiotic relationship with A. hypochondriacus to alleviate the environmental stress it experiences and improve rhizosphere soil quality. This study aims to evaluate the potential for AMF to form a symbiotic relationship with salt-tolerant Amaranthaceae plants. By measuring the colonization rate, biomass, and rhizosphere soil chemical properties of the plants, we assessed the response of A. hypochondriacus to AMF under different concentrations of saline-alkali stress. The results showed that Rhizophagus intraradices could form a good symbiotic relationship with A. hypochondriacus and reduce the rhizosphere soil pH and increase the effective nutrient content.