Estimating passenger movement in transportation networks is a critical aspect of public transportation systems. It allows for a greater understanding of traffic patterns, as well as efficient system evaluation and monitoring. It could also help with precise timing to emergencies or important events, as well as the improvement of urban transport system weaknesses and service quality. The number of transfer passengers demand in Istanbul, Turkey's biggest and most developed metropolis, was used to construct a real-world forecasting model in this study. The number of transfer passengers has been forecasted using popular machine learning methods such as kNN (k-Nearest Neighbours), LR (Linear Regression), RF (Random Forest), SVM (Support Vector Machine), XGBoost and MLP. The dataset utilized is made up of hourly passenger transfer counts gathered at two public transportation transfer stations in Istanbul in January 2020. Using MSE, RMSE, MAE and R2 parameters, each model's experimental data have been thoroughly evaluated. MLP has more successfully other machine learning algorithms in the majority of transportation lines, according to the experimental results.