Biogas slurry is a good source of plant nutrients and replacing chemical fertilizers with biogas slurry can not only achieve resource utilization of slurry, but also reduce the amount of fertilizer. In this study, biogas slurry was applied in the production of oil-seed rape, it was attempted to investigate the effects of slurry on yield and quality of rape, furthermore to find the optimum rate of biogas slurry. The results showed that the protein, iron (Fe), manganese (Mn), copper (Cu), and zinc (Zn) contents in rapeseed increased, the oleic acid, calcium (Ca) and magnesium (Mg) contents in rapeseed firstly increased and then decreased with the increased rate of slurry. Meanwhile, the erucic acid, glucosinolate and oil contents showed a tendency of decrease with the increased rate of slurry. When the rate of slurry was in the range of 78,750 kg hm−2 to 101,250 kg hm−2, the yield reached a relatively high level, which was in the range of 2817kg hm−2 to 3433 kg hm−2.