Quality Of Preparation Research Articles

Influence of background music on stress reduction and impact on performances during students' simulation exercises.

The acquisition of manual skills is essential in preclinical dental training. Background music improves the learning of many manual skills, though we found no data on the consequences of background music on preclinical manual skills training of dental students. The first aim of this project was to explore whether listening to slow background music could reduce the stress of students when learning how to perform cavity preparations and restorations in a simulation laboratory. The second aim of this study was to determine the impact of slow background music on the quality and time used during cavity preparation. We invited all of the 40 third-year dental students to participate in the study, of whom 88% chose to anonymously fill in questionnaires on their subjective evaluations of the effects of slow background music on the stress or anxiety levels experienced during the course. Twenty-four students further volunteered to participate in a cross-over study on the impact of slow background music on the quality of and time used during cavity preparation. The overall satisfaction with the slow background music was high. In particular, the music reduced stress but also increased motivation to learn and practice. Communication in the classroom went well despite the music. Time use and quality of cavity preparation were enhanced. This study lends support to the use of slow background music in preclinical cariology training, as it appeared to have helpful effects on dental skills education and practice.

Efficacy of single-versus split-dose polyethylene glycol for colonic preparation

Background: Before a colonoscopy, polyethylene glycol (PEG) is often used to prepare the colon. PEG dosage recommendations are still up for discussion. In this randomized controlled experiment, the effectiveness and acceptability of PEG administered in single vs. split doses for colonic preparation was compared. Methods: A total of 120 individuals were randomized to either split-dose PEG for colonic preparation or a single-dose PEG at Department of Gastroenterology, Hayatabad medical complex. The Boston Bowel Preparation Scale (BBPS) was used for assessing the rate of bowel cleaning for the colonoscopy. A visual analog scale was used to evaluate the preparation's acceptability and tolerability. Results: The quality of colonic preparation was considerably greater in the split-dose PEG group than in the single-dose PEG group, as shown by the BBPS score, which was significantly higher in the split-dose PEG group (7.5±1.2) than in the single-dose PEG group (6.3±1.3) (P0.001). Patient satisfaction did not vary significantly between the two groups, but the split-dose PEG group had a higher completion rate of colonoscopies (97% vs. 95%) as well as greater patient acceptability and tolerance.

Use of Smartphone Applications to Augment Colonoscopy Preparation Instructions and Effect on Quality of Colonoscopy Preparation and Adenoma Detection Rate –

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The Colonoscopy Cookbook: Analysis of effect of a recipe resource on quality and experience of bowel preparation in colonoscopy.

Background High-quality bowel preparation for a colonoscopy improves identification of early lesions in the large bowel, decreases procedure time and increases intervals between colonoscopies. Current recommendations advise a low-residue diet in the days leading up to colonoscopy to improve quality of preparation. This study prepared and provided a recipe resource to patients undergoing colonoscopy and assessed the quality of bowel preparation and patient experience. Patients and methods A "Colonoscopy Cookbook" resource of recipes that comply with the preoperative diet recommendations was created and added to routine preoperative information given to patients undergoing elective colonoscopies at a regional Australian hospital over a 12-month period. Endoscopic reports were reviewed for each case and quality of bowel preparation was classified as "adequate'' or "inadequate". Data collected were compared to a representative local cohort from 2019. Results Procedure reports from 96 patients who were provided with the resource were compared with 96 patients who were not. Adequate bowel preparation was nine times as likely when the resource was available (odds ratio 8.54, 95 % confidence interval: 2.85 to 25.60, P < 0.001) compared to when it was not. The patient experience was assessed using a post-procedure survey, which demonstrated a positive experience in recipe preparation. Most patients would use the resource prior to future colonoscopies. Conclusions Further randomized controlled trials are required to validate this scoping review. Pre-procedure recipe resources may improve quality of bowel preparation in patients undergoing colonoscopy.

Formulation of Peel-Off Gel Mask from of Carbopol (Daucus carota L) Skin Extract as Antioxidant with variations of Carbopoly Base 940 and Propylene Glycol

Antioxidant compounds reduce ageing of the skin. Carrot peels contain antioxidant compounds. This research aimed to determine whether the red dragon fruit peels can be made into preparations like peel-off mask gel with variations in carbopol 940 and propylene glikol as the influence of variations in the physical basis of the quality of preparation and antioxidant activity. Carrot peel extraxtion by a maseration method using 70% ethanol. Peel-off mask gel made with a variable concentration of carbopol 940 and propylene glycol which is 0.5%: 5%; 0.5%: 7.5%; 0.5%: 10%; 1%: 5%; 1%: 7.5%; and 1%: 10%. The DPPH (1,1-dyphenil-2-picrylhydrazyl) method was used for the antioxidant test. The evaluation is used for observing the physical properties, which include organoleptic, time to dry, viscosity, spreading, and pH, with antioxidant activity of each formula. The results showed that the carrot peels can be made into a prepared peel-off mask gel. The low amount of propylene glycol and high amount of carbopol 940 affected the quality of the preparation. It had a high viscosity, low dispersive power, an acid pH, dried out slowly, and had low antioxidant activity.

Efektifitas Penggunaan Deterjen Laundry Cair sebagai Agen Deparafinisasi pada Sediaan Ginjal Mencit (Mus musculus)

Deparaffinization is one of the stages of tissue processing, which aims to dissolve paraffin for full-color application. Deparaffinization can only be done by xylol to dissolve paraffin as fat. Liquid laundry detergent has the main component in the form of a surfactant which has the property of removing types of lipids by dissolving and emulsification because xylol has carcinogenic properties, flammable properties, and is relatively expensive. This study aimed to describe the quality of tissue preparations using liquid laundry detergents 1.5%, 3%, and 4.5% as deparaffinized agents and also to describe and compare several concentrations of the most effective laundry detergents as deparaffinized agents. Results from the preparation quality using xylol as a deparaffinized agent by conducting an experimental study with a cross-sectional design showed promising results. The quality of preparations using 1.5% liquid laundry detergent got poor results. The quality of preparations using 3% liquid laundry detergent got poor results. The quality of preparations using 4.5% liquid laundry detergent got good results. Based on the results of the Kurskal Wallis test with a significance value of p &lt;0.05, which indicates that H0 is rejected, the conclusion that can be drawn is that there is an unfavorable difference in results between xylol and liquid laundry detergent as a deparaffinized agent.

Shaping ability of WaveOne Gold and OneReci by using two apical sizes: a micro-computed tomographic assessment.

OneReci (MicroMega, Besançon, France) is a recently introduced single-file reciprocating system with scarce information revealed on its shaping ability. This study aimed to compare the shaping abilities of OneReci and a well-documented single-file reciprocating system WaveOne Gold (WOG; Dentsply Maillefer, Ballaigues, Switzerland) and evaluate the effect of increased apical enlargement on the preparation quality, using micro-computed tomography (micro-CT). After an initial micro-CT scanning, twenty mesial root canals of mandibular molars were anatomically matched. The canals were assigned to two experimental groups (n=10), using OneReci or WOG in different canals of the same root. The glide paths were created, and root canals were prepared twice, using size 25 and 35 instruments of the systems, respectively. The specimens were scanned with micro-CT after each preparation. The increase in canal volume, amount of dentin removal, unprepared root canal surface, canal transportation, centering ratio and preparation times were assessed. The data were analysed with independent sample t-tests, variance analyses, Friedman and Mann-Whitney U tests. The significance level was set at 5%. Each preparation increased the canal volume and dentin removal while decreasing the unprepared root surface. The difference between the systems became significant after preparation with size 35 instruments (p<0.05). Regarding canal transportation and centering ratio, the difference was insignificant (p>0.05). The first preparation step (glide path + size 25 instrument) was significantly faster in the OneReci group (p<0.05). Preparation with size 25 instruments of the systems appeared to be safe with similar shaping performances. Larger apical preparation promoted significantly higher dentin removal, volume increase, and prepared surface area in WOG.

Pressure-controlled microfluidics for automated single-molecule sample preparation

Sample preparation is a crucial step in single-molecule experiments and involves passivating the microfluidic sample chamber, immobilizing the molecules, and setting experimental buffer conditions. The efficiency of the experiment depends on the quality and speed of sample preparation, which is often performed manually and relies on the experience of the experimenter. This can result in inefficient use of single-molecule samples and time, especially for high-throughput applications. To address this, a pressure-controlled microfluidic system is proposed to automate single-molecule sample preparation. The hardware is based on microfluidic components from ElveFlow and is designed to be cost-effective and adaptable to various microscopy applications. The system includes a reservoir pressure adapter and a reservoir holder designed for additive manufacturing. Two flow chamber designs Ibidi µ-slide and Grace Bio-Labs HybriWell chamber are characterized, and the flow characteristics of the liquid at different volume flow rates V̇ are simulated using CFD-simulations and compared to experimental and theoretical values. The goal of this work is to establish a straightforward and robust system for single-molecule sample preparation that can increase the efficiency of experiments and reduce the bottleneck of manual sample preparation, particularly for high-throughput applications.

Polyps are detected more often in early colonoscopies

Objective To examine the time variation in polyp detection for colonoscopies performed in a tertiary hospital and to explore independent factors that predict polyp detection rate (PDR). Methods Data on all patients who underwent colonoscopy for the diagnostic purpose at our endoscopy center in Zhongnan Hospital of Wuhan University from January 2021 to December 2021 were reviewed. The start time of included colonoscopies for eligible patients was recorded. PDR and polyps detected per colonoscopy (PPC) were calculated. The endoscopists’ schedules were classified into full-day and half-day shifts according to their participation in the morning and afternoon colonoscopies. Results Data on a total of 12116 colonoscopies were analyzed, with a PDR of 38.03% for all the patients and 46.38% for patients ≥50 years. PDR and PPC significantly decreased as the day progressed (both p < .001). For patients ≥50 years, PDR declined below 40% at 13:00–13:59 and 16:00–16:59. The PDR in the morning was higher than that in the afternoon for both half-day (p = .019) and full-day procedures (p < .001). In multivariate analysis, start time, patient gender, age, conscious sedation, and bowel preparation quality significantly predicted PDR (p < .001). Conclusions The polyp detection declined as the day progressed. A continuous work schedule resulted in a subpar PDR. Colonoscopies performed in the morning had a higher PDR than that in the afternoon. Patient gender, age, conscious sedation, and bowel preparation quality were identified as the independent predictors of PDR.

Design of a Convolutional Neural Network as a Deep Learning Tool for the Automatic Classification of Small-Bowel Cleansing in Capsule Endoscopy.

Background and objectives: Capsule endoscopy (CE) is a non-invasive method to inspect the small bowel that, like other enteroscopy methods, requires adequate small-bowel cleansing to obtain conclusive results. Artificial intelligence (AI) algorithms have been seen to offer important benefits in the field of medical imaging over recent years, particularly through the adaptation of convolutional neural networks (CNNs) to achieve more efficient image analysis. Here, we aimed to develop a deep learning model that uses a CNN to automatically classify the quality of intestinal preparation in CE. Methods: A CNN was designed based on 12,950 CE images obtained at two clinical centers in Porto (Portugal). The quality of the intestinal preparation was classified for each image as: excellent, ≥90% of the image surface with visible mucosa; satisfactory, 50-90% of the mucosa visible; and unsatisfactory, <50% of the mucosa visible. The total set of images was divided in an 80:20 ratio to establish training and validation datasets, respectively. The CNN prediction was compared with the classification established by consensus of a group of three experts in CE, currently considered the gold standard to evaluate cleanliness. Subsequently, how the CNN performed in diagnostic terms was evaluated using an independent validation dataset. Results: Among the images obtained, 3633 were designated as unsatisfactory preparation, 6005 satisfactory preparation, and 3312 with excellent preparation. When differentiating the classes of small-bowel preparation, the algorithm developed here achieved an overall accuracy of 92.1%, with a sensitivity of 88.4%, a specificity of 93.6%, a positive predictive value of 88.5%, and a negative predictive value of 93.4%. The area under the curve for the detection of excellent, satisfactory, and unsatisfactory classes was 0.98, 0.95, and 0.99, respectively. Conclusions: A CNN-based tool was developed to automatically classify small-bowel preparation for CE, and it was seen to accurately classify intestinal preparation for CE. The development of such a system could enhance the reproducibility of the scales used for such purposes.

Patient- and cycle-specific factors affecting the outcome of frozen–thawed embryo transfers

PurposeThis study attempted at identifying the main parameters influencing the outcome of frozen embryo transfers.MethodsThis is a single-center retrospective cohort study of 830 frozen-embryo-transfer cycles performed at a German university hospital from January 2012 to December 2016. Main outcome parameters were the clinical pregnancy and live birth rate. Twelve patient- and cycle-dependent factors were analyzed in terms of their influence on the outcome of frozen embryo transfers. Multivariate logistic regression analysis was used for the modelling of the dependency of the different parameters on outcomes.ResultsThe clinical pregnancy rate in our study was 25.5%, the live birth rate was 16.1% with an average maternal age of 34.2 years at the time of the oocyte retrieval. In the univariate analysis age, number of transferred embryos, blastocyst versus cleavage stage transfer, embryo quality and mode of endometrial preparation affected the birth rate significantly. The birth rate after artificial endometrial preparation was significantly lower than the birth rate after transfers in modified natural cycles (12.8 versus 20.6% with p = 0.031). The multivariate logistic regression analysis showed a significant independent influence of age, number of transferred embryos, culture duration and mode of endometrial preparation on the frozen embryo transfer success rates. Body mass index, nicotine abuse, a history of PCO syndrome or endometriosis and the co-transfer of a second poor-quality embryo to a good-quality embryo appeared to be irrelevant for the outcome in our collective.ConclusionAge, number of transferred embryos, embryo culture duration and the mode of endometrial preparation are independent predictive factors of frozen embryo transfer outcomes.

Effect of acuity level and patient characteristics on bowel preparation quality: a retrospective cohort study of inpatient colonoscopies

Background and aimsColonoscopy is the primary method to detect mucosal abnormalities in the colon, rectum, and terminal ileum. Inadequate bowel preparation is a common problem and can impede successful visualization during colonoscopy. Although studies identified hospitalization as a predictor of inadequate bowel preparation, acuity of care vary greatly within this patient population. The current study aims to examine the effect of patient characteristics and care level predictors on inadequate bowel preparation quality within the inpatient setting.MethodsThis retrospective study was conducted in a single urban level 1 trauma medical center and included adult patients undergoing diagnostic colonoscopy while admitted in the hospital from January 1, 2015 to June 30, 2020. We examined the level of inpatient care between the General Medical Floor (GMF), Intensive Care Units (ICU) and Telemetry Unit (TU) and assessed this association with bowel preparation quality, adjusting for known and unknown predictors.ResultsOf 538 patients undergoing colonoscopy, 47.4% were admitted into TU, 43.7% into GMF and 8.9% into ICU. For the entire sample, 72.7% of patients achieved good or excellent preparation and quality of bowel preparation differed by care level (P = 0.01). Patients from the critical care units were less likely to achieve adequate bowel preparation when compared to GMF (Odds Ratio [OR] 0.36; 95% Confidence Interval [CI] 0.17,0.77), after adjusting for patient characteristics, medications, physical status, and preparation regimen. No significant difference in Bowel Preparation Quality (BPQ) was identified between patients from GMF and TU (OR 0.96; 95%CI 0.61, 1.52). Furthermore, adequate BPQ was associated with withdrawal time and cecal intubation, but not higher adenoma detection rates.ConclusionsResults suggest the ICU setting is an independent predictor for inadequate bowel preparation and patients with prior opioid and laxative use may be more likely to have inadequate bowel preparation in the hospital. Future interventions should prioritize preprocedural clinician meetings for critical care unit patients, including a more detailed readiness assessment and thorough medication history.

Analysis of the pharmacokinetic properties of a native drug on the basis of a complex of sorbents

Absolutely all preclinical studies involve three groups of experiments: preclinical studies of the pharmacokinetics of the substance, including toxicokinetics, preclinical studies of the pharmacokinetics of the drug and the clinical study of pharmacokinetics on target animals. Pharmacokinetic studies of native drugs are complex because they usually involve the administration of complex components. Thus, only the use of the whole complex of pharmacognostic, chemical, spectral, physicochemical and biological methods can provide an objective assessment of the authenticity and quality of medicinal herbal raw materials and medicinal herbal preparations. The study used Wistar white rats, weight 150-160g according to the Guidelines for Evaluation of Medicinal Products (3).The purpose of the study was to evaluate the pharmacokinetic properties of a new feed additive in the form of a premix of sorbents and phytocompositions: milk thistle (DV-silibinin, Pharmcenter VILAR CJSC), thyme (DV-timol, Akhma100). Supplements in the form of finished dosage forms were standardized for the active substance, and have a constant composition. Thus, it allowed to create a study design for a new substance in one animal species. Since the composition of the sorption component was studied earlier, the introduced substances were considered separately and in combination with sorbents.After a single injection, the linearity of the pharmacokinetics was assessed, based on the administered different doses, the analysis of the main biochemical parameters of the blood was carried out, and the bioavailability was also calculated. Thus, the new premix based on sorbents and plant substances in the form of milk thistle and thyme does not have a negative effect on the body of laboratory animals. As a result of the studies, LD50 and LD100 were not established for the drug, the drug is classified as low-toxic, belongs to the fourth hazard class according to GOST 12.1.007-76 "Harmful substances", which allows further toxico-therapeutic tests.

Express-diagnostics of the properties of ЫРЫ materials using chrono-topographic analysis of thermal vision data

Subject of research: the article is devoted to the development of a methodology for express diagnostics of material properties based on the measurement of macrokinetic parameters of the SHS process.&#x0D; Purpose of research: plotting the dependence of the microhardness of SHS products on the propagation velocity of the SHS wave and its temperature.&#x0D; Methods and objects of research: the research objects were the SHS process in the Ni-Al system and the reaction products; To measure the propagation velocity of a high-temperature synthesis wave, an original method of chrono-topographic analysis of thermal imaging data was used; temperature control was carried out by an original method of spectral-brightness pyrometry. The study of the microhardness of experimental samples was carried out using an FM-800 microhardness tester.&#x0D; Main results of research: in the course of experimental studies, it was shown that the error in determining the propagation velocity of the SHS wave by the method of chrono-topographic analysis of thermal imaging data is 0.1 %, and the error in temperature measurement by the method of spectral-brightness pyrometry is estimated at 0.5 %. It is concluded that the main factor influencing the total error in the SHS wave propagation velocity in the experiment is the inaccuracy of setting the charge parameters for a set of samples. The absolute value of the total error was 3.2 %. A diagnostic trajectory has been constructed that makes it possible, based on the SHS propagation rate and reaction temperature, to evaluate the microhardness of the product and draw a conclusion about the quality of charge preparation for the production of a material with desired properties.

Teleconsultation in pediatric anesthesia: First assessment of feasibility, quality, and satisfaction in France.

The COVID-19 pandemic has prompted the development of anesthesia teleconsultation in many countries. In pediatric anesthesia, data about anesthesia teleconsultation are scarce. The main objective of this prospective descriptive study was to provide an evaluation of the feasibility of pediatric anesthesia teleconsultation. Perception of the safety and quality, parental and medical satisfaction were also assessed. From September to December 2020, patients undergoing a pediatric anesthesia teleconsultation in Toulouse University Hospital, using the TeleO™ dedicated teleconsultation platform were prospectively included. Feasibility was defined as the rate of anesthesia teleconsultations successfully performed using the TeleO™ platform alone. Questionnaires regarding the quality, safety, and satisfaction were filled in by physicians and families. A total of 114 children (3 months-17 years) were included in the study. Feasibility was 82%, failure was mainly caused by technical issues. Physicians estimated that the safety and quality of anesthetic preparation were optimal in 100% of cases. Anesthetists were satisfied (VAS ≥70/100) with the medical, technical, and relational (child/parents) aspects of anesthesia teleconsultation in 91%, 64%, and 84%/90% of cases respectively. Almost all parents (97%) stated that they would agree to anesthesia teleconsultation for a future procedure. In this first assessment, pediatric anesthesia teleconsultation appears to be feasible, with high rates of medical and parental satisfaction. Physicians' perception of the safety and quality of this process were positive. Improving the technical process might be a key determinant to promote further development of pediatric anesthesia teleconsultation.

Определение влияния подготовки поверхности металла на стойкость эпоксидно-песчаного покрытия к воздействию агрессивных сред

The composition and features of the manufacture of specimens of epoxy-sand composite used during the field repair of road construction machines are considered. The results of determining the influence of the preparation quality of a metal surface on resistance of the epoxy-sand coating to aggressive media are presented. It is shown that the quality of the filler preparation does not affect the coating resistance to aggressive media. It was found out that specimens with a pre-treated surface before applying the epoxy-sand composite coating showed the best resistance to aggressive media.

MENTAL PREPARATION WOMEN AT HIGH-RISK PREGNANCY

Background: Pregnancy becomes risk period in woman’s life which need to make adjustment in physical and psychological. Improper handling can cause serious problem. Women need to be readiness before entry to next phase become a mother. Objective: To reveal about mental readiness of pregnant women at high-risk pregnancy. Method: This paper was review which supported by literature review from PubMed, Medline and Cinahl database research published journal from 2017-2021 which talk about pregnant women at high-risk during pregnancy and the fetus. Results: 15-20% of pregnancies are high risk; pregnancies are complicated by the presence of a high risk or serious condition that affects the outcome of the mother and the fetuses. Women with high-risk pregnancies had 5.2x greater anxiety than women with low-risk pregnancies. High-risk pregnancies require more intensive or frequent treatments. Mental preparations need to be supported by many supporting system and holistic interventions. Conclusion: High-risk pregnancy and childbirth are two risk factors associated with anxiety during pregnancy. The mental preparation of pregnant women needs to be supported with adequate mental health to minimize adverse outcomes, maintain normal social relationshop and interactions, physical activity, oriented interventions to bring better quality of preparation. Keywords: mental, high-risk pregnancy, childbirth.

UJI EFEKTIVITAS ANTIBAKTERI SEDIAAN SABUN PADAT TRANSPARAN EKSTRAK ETANOL DAUN CABE RAWIT (Capsicum Frutescens L.) TERHADAP BAKTERI Propionibacterium acne

Cayenne pepper leaves (Capsicum frutescens L.) can traditionally be used as an alternative treatment for acne from natural ingredients. This study aims to determine the effectiveness and concentration of the transparent solid soap formulation ethanol extract of cayenne pepper (Capsicum frustescens L.) leaves in inhibiting the growth of acne-causing bacteria (Propionibacterium acne). The method used in this research is experimental. Making cayenne pepper leaf extract (Capsicum Frutescens L.) by maceration method using 96% ethanol then making a transparent solid soap formulation and testing the effectiveness of antibacterial using disc paper diffusion method against Propionibacterium acnes bacteria by measuring the diameter of the inhibition zone for bacterial growth so that it can known Minimum Inhibitory Concentration (MIC). The concentration of Cayenne pepper leaf extract and the transparent solid soap formula used were (FI) 1%; (FII) 2%; (FIII) 3% and blanks (K+) Ranee soap and (K-) extractless soap. Evaluation of the physical quality of transparent solid soap preparations includes: organoleptic test, pH test, foam power test. From the test results, the average transparent solid soap preparation with a concentration of 1% produced an inhibition zone of 10.7 mm, a concentration of 2% produced an inhibition zone of 11.8 mm, and a concentration of 3% produced an inhibition zone of 12.6 mm. Minimum Inhibitory concentration (MIC) which produced the best ethanol extract of cayenne pepper (Capsicum frutescens L.) leaves was found at a concentration of 1% with a Width of Inhibitory Power (LDH) of 10.7 mm. The One Way Anova test obtained a significance value of 0.000 &lt;0.05, so the results were significant, so there were significant differences between treatments. This means that the use of cayenne pepper leaf extract (Capsicum frutescens L.) has an effect on the growth of Propionibacterium acnes bacteria.

A study to assess the Nurses knowledge &amp; compliance to the protocol of adequate bowel preparation for colonoscopy

Introduction: Cleansing the colon before a colonoscopy is called bowel preparation, or “prep.” It involves taking medication that causes frequent, loose bowel movements to empty the colon. The medication is taken by mouth and typically comes in liquid form. Bowel cleansing is one of the most important parameters included in the evaluation of colonoscopy quality. The available evidence suggests that inadequate bowel preparation reduces the diagnostic yield of colorectal neoplasia and increases post-colonoscopy colorectal cancer risk. Nowadays, up to 30% of patients referred for colonoscopy have poor bowel cleansing. Recently, several studies have analyzed the risk factors for inadequate bowel cleansing as well as the strategies to optimize bowel preparation. Poor bowel preparation has been shown to be associated with low lower-quality dilators of colonoscopy performance. Patients with Personal History of Inadequate Bowel Preparation, Diabetes Mellitus, Chronic Constipation, and a History of Colorectal Resection are at risk of inadequate bowel preparation for colonoscopy. Methodology: In November 2022, there was an increase in consultant complaints about inadequate Bowel preparation for the patient in the ward &amp; ICU which impacted on proper diagnosis of the actual reason the patient took admitted at Apollo Hospitals, Bhubaneswar. So on basis of the complaints, a quasi-experimental group pre-test was conducted &amp; found the less compliance. To look forward to checking the compliances, training &amp; educational materials were prepared on basis of protocol of adequate bowel preparation for colonoscopy (education materials contained agents, methods (single/split) of preparation, dietary patterns, stool consistency assessment) &amp; started implement from December 2022 onwards in both one to one &amp; group teaching. And by end of February 2023, a post-test was conducted to assess the improvement of knowledge of Nurses. To collect the data of their practices on adequate bowel preparation a structured PIP (performance Improvement Project) tool was used &amp; total sample took 100 assigned Register Nurses who all were assigned the In-patients for Colonoscopy. Results: With constant reinforcement, educational materials on adequate Bowel preparation of Colonoscopy &amp; knowledge assessment of assigned nurses made an impact on the effective intervention in adequate Bowel preparation. The impact of compliance to assess the knowledge &amp; interventions of nurses in adherence to the protocol of Adequate Bowel preparation for colonoscopy. The knowledge of Nurses found less compliance in the Pre-Test which was conducted after consultant’s complaints in the month of November 2022 with 55.83% &amp; then after training &amp; educational materials were prepared on basis of protocol of adequate bowel preparation for colonoscopy {education materials contained agents, methods (single/split) of preparation, dietary patterns, stool consistency assessment)} &amp; started implement from December 2022 onwards in both one to one &amp; group teaching. And by end of February 2023, a post-test was conducted to assess the improvement of knowledge of Nurses &amp; found compliance percentage of 78.33%. And Compliance with adherence to the proper method of Bowel Preparation Protocol found compliances month on month, in December 2022 it was 30.29%, in January 2023 it improves to 55.26% &amp; reaches with compliances of 78.94% by the end of February 2023. Conclusion: In patients under the care of nurses who underwent enhanced education on bowel preparation had significantly better bowel preparation quality and higher compliance rates than those in inpatients of the control group. Nurses’ education was identified as an independent risk factor for bowel preparation quality. To make bowel preparation adequate &amp; sufficient frequent updating on knowledge of the recruited nurses need to be done on regular basis.

Experience of Klebsiella pneumoniae genome sequencing using the short read method on the Illumina platform

Objective. To review the main stages of Klebsiella pneumoniae genome sequencing using the Illumina short-read method and describe the peculiarities of sample library preparation and analysis of the obtained data.Materials and methods. Deoxyribonucleic acid (DNA) for high-throughput sequencing was isolated from Klebsiella pneumoniae cultures. Sample preparation was performed according to the manufacturer’s instructions for the Nextera XT DNA Library Prep kit. Sequencing was performed on an Illumina MiSeq platform using a 2x151 cartridge. Genome assembly to the contigs was performed using the SPAdes Genome Assembler application on the Illumina BaseSpace Sequence Hub service and a set of programs in a Linux environment. The quality of genome assembly was assessed using the QUAST service.Results. Genome sequencing of K. pneumoniae culture samples was performed, followed by an evaluation of the quality of the launch, assembly of the genome, and determination of its main parameters.Conclusion. The main steps of K. pneumoniae genome sequencing have been considered using the short-read method on the Illumina platform. The main parameters for assessing the quality of sample preparation, launch and genome assembly are described.