Plant Quality Research Articles

Non-Destructive Prediction of Rosmarinic Acid Content in Basil Plants Using a Portable Hyperspectral Imaging System and Ensemble Learning Algorithms

Rosmarinic acid (RA) is a phenolic antioxidant naturally occurring in the plants of the Lamiaceae family, including basil (Ocimum basilicum L.). Existing analytical methods for determining the RA content in leaves are time-consuming and destructive, posing limitations on quality assessment and control during cultivation. In this study, we aimed to develop non-destructive prediction models for the RA content in basil plants using a portable hyperspectral imaging (HSI) system and machine learning algorithms. The basil plants were grown in a vertical farm module with controlled environments, and the HSI of the whole plant was captured using a portable HSI camera in the range of 400–850 nm. The average spectra were extracted from the segmented regions of the plants. We employed several spectral data pre-processing methods and ensemble learning algorithms, such as Random Forest, AdaBoost, XGBoost, and LightGBM, to develop the RA prediction model and feature selection based on feature importance. The best RA prediction model was the LightGBM model with feature selection by the AdaBoost algorithm and spectral pre-processing through logarithmic transformation and second derivative. This model performed satisfactorily for practical screening with R2P = 0.81 and RMSEP = 3.92. From in-field HSI data, the developed model successfully estimated and visualized the RA distribution in basil plants growing in the greenhouse. Our findings demonstrate the potential use of a portable HSI system for monitoring and controlling pharmaceutical quality in medicinal plants during cultivation. This non-destructive and rapid method can provide a valuable tool for assessing the quality of RA in basil plants, thereby enhancing the efficiency and accuracy of quality control during the cultivation stage.

Night-Flowering Jasmine: A Review of Its Morphology and Pharmacological Activity

Known by many as Parijat or Night flowering Jasmine, Nyctanthes arbortristis is a mesmerising plants that are native to the subcontinent of India. This review paper provides an extensive summary of the medical, botanical, and Nyctanthes arbortristis's decorative attributes based on the body of current literature. The plant qualities of the plant, such as its distribution, growth patterns, and morphology are outlined. Nyctanthes's therapeutic qualities arbortristis, which have been thoroughly studied observed in a number of studies, are investigated. These characteristics include analgesic, a antiinflammatory, and hepatoprotective, antiviral, antioxidant, and antitumor actions. Additionally, the decorative application of Nyctanthes The discussion of an rbortristis emphasises its beauty attractiveness and potential for gardening and landscaping objectives. The purpose of this review paper is to offer a thorough comprehension of the diff erent features that make Nyctanthes arbortristis function as a useful.

Genome-wide profiling of soybean WRINKLED1 transcription factor binding sites provides insight into seed storage lipid biosynthesis

Understanding the regulatory mechanisms controlling storage lipid accumulation will inform strategies to enhance seed oil quality and quantity in crop plants. The WRINKLED1 transcription factor (WRI1 TF) is a central regulator of lipid biosynthesis. We characterized the genome-wide binding profile of soybean (Gm)WRI1 and show that the TF directly regulates genes encoding numerous enzymes and proteins in the fatty acid and triacylglycerol biosynthetic pathways. GmWRI1 binds primarily to regions downstream of target gene transcription start sites. We showed that GmWRI1-bound regions are enriched for the canonical WRI1 DNA binding element, the ACTIVATOR of Spomin::LUC1/WRI1 (AW) Box (CNTNGNNNNNNNCG), and another DNA motif, the CNC Box (CNCCNCC). Functional assays showed that both DNA elements mediate transcriptional activation by GmWRI1. We also show that GmWRI1 works in concert with other TFs to establish a regulatory state that promotes fatty acid and triacylglycerol biosynthesis. In particular, comparison of genes targeted directly by GmWRI1 and by GmLEC1, a central regulator of the maturation phase of seed development, reveals that the two TFs act in a positive feedback subcircuit to control fatty acid and triacylglycerol biosynthesis. Together, our results provide unique insights into the genetic circuitry in which GmWRI1 participates to regulate storage lipid accumulation during seed development.

The Effect of Macro-Micro Nutrient Fertilizers and Plant Spacing on the Growth (Brassica juncea L.)

Brassica juncea L. is a much-loved vegetable species in Indonesia. To improve the quality and quantity of mustard plants, agricultural intensification efforts are needed through macro-micro fertilisers that maintain the balance of soil nutrients. This study aims to determine the response of mustard plants to the application of macro-micro fertilisers. The research was conducted in Claket Village, Pacet District, Mojokerto Regency at an altitude of 470 masl with a daily temperature of 21-30°C. The method used was a factorial Randomised Group Design (RAK) with two factors (fertiliser dose and spacing) and three replications. Variables observed included increase in plant height, number of leaves, wet weight, dry weight, and water content. Data analysis used F test and BNJ 5%. The results showed that the interaction of macro-micro fertiliser dose and planting distance gave a significant effect on all variables, except dry weight due to high water content in mustard greens.

Inoculation of Arthrobacter sp. improves the growth of Acanthocalycium sp. and the fruits nutraceutical quality and the flowers longevity

Research objective: The aim of this work is on the inoculation in the growing medium of Arthrobacter sp. and its effects on the growth, flower quality and longevity of cactus plants. To this end, the study of biometric parameters, i.e. plants and flowers (height, weight, number and duration), as well as root growth (weight), and the presence of disease were quantified as measures of plant productivity and compared with those obtained from non-inoculated plants. The use of alginate microspheres as a means of bacterial inoculation was also considered. Materials and Methods: Seedlings of Acanthocalycium (2 years old) Ferrarii, A. Glaucum and A. Violaceum were immediately planted in pots after purchase, in substrate containing (sand 20%, pumice 20% and peat 60%; pH 7; electrical conductivity 0.7 dS/m; total porosity 80% (v/v). A randomised complete block design was applied for the experiments: the pots were randomly divided into two series, one series was inoculated with the bacteria and the other non-inoculated was used as a control. Each month, plant growth was assessed according to (1) plant height, (2) vegetative and root weight, (3) number of flowers per plant (4) flower duration. In addition, the occurrence of possible diseases, in particular Fusarium sp. and Verticillium sp., was assessed. Total flavonoids, total phenols and antioxidant activity were also analysed. Results and Discussion: The experiment showed that the use of Arthrobacter sp. can indeed significantly improve the vegetative and root growth of Acanthocalycium sp. cacti. In addition, there was a significant improvement in the number and floral longevity as well as in the phenol, flavonoid and antioxidant content of the fruits of the inoculated plants compared to the untreated control. The experiment also showed that mortality due to the pathogens Fusarium sp. and Verticillium sp. was significantly reduced in the treated plant. In agreement with these authors, we found that PGPB treatments increased agronomic parameters and improved Fusarium and Verticillium resistance. In general, the application of biostimulants can increase the synthesis of bioactive compounds in plants by increasing resistance to phytopathologies. Inoculation with Arthrobacter sp. led to a significant reduction in the number of dead plants with respect to the diseases analysed. As a result of the stimulating action demonstrated in Acanthocalycium, bioproducts suitable for nutraceutical purposes may be developed in the future. Therefore, new microbe-assisted technologies can help plants to resist stress conditions, improving their tolerance and productivity. Conclusions: Due to its ‘multifunctionality’, the genus Acanthocalycium is considered as one of the species of the future, for ornamental use and medicinal aspects, and new results may help reveal its potential in the context of the bio-economy and circular economy. As natural resources and cultural practices are crucial in defining the quality of flowers when destined for food/nutraceutical applications, the inoculation of Acanthocalycium with Arthrobacter sp. can be envisaged to provide better plant growth under conditions of environmental stress, or as a soil fertiliser, but also to improve the synthesis of natural products used for therapeutic applications.

Diagnosis and prognosis of soybean roaster failures using particle swarms

In industry, the monitoring and diagnosis of production processes are crucial issues in ensuring plant reliability, performance and quality. In particular, food processing operations, such as coffee roasting, are subject to numerous risks of failure that can impact on productivity and the quality of the final product. In this context, the main objective of this study was to develop an innovative method for the diagnosis and prognosis of failures in a coffee roasting process. The proposed method differs from standard approaches by using the particle swarm optimization (PSO) algorithm applied to the analysis of signatures of key process variables. This new approach has improved fault detection, with a recognition rate of over 90% for the main types of fault identified, such as heating problems, air obstructions or leaks. In addition to diagnostics, the method has also demonstrated its effectiveness in prognosticating the state of health of the process, with an average error on the prediction of remaining service life reduced to 15%, compared with 35% for fixed-threshold methods. This work has therefore enabled us to develop an innovative method offering superior performance to standard approaches for the diagnosis and prognosis of failures in the roasting process.

Scent Knows Better: Utilizing Volatile Organic Compounds as a Robust Tool for Identifying Higher Cannabidiol- and Tetrahydrocannabinol-Containing Cannabis Cultivars in Field Conditions.

The primary cannabinoids cannabidiol (CBD) and tetrahydrocannabinol (THC), found in cannabis, are known to originate from genetic diversity, resulting in distinct characteristics. This study aimed to identify VOC markers to distinguish between higher CBD and THC cannabis cultivars under field conditions. Among the 58 VOCs, β-caryophyllene and α-humulene were primary VOCs across all cannabis cultivars. Intriguingly, certain terpene VOCs exhibited contrasting trends between higher CBD and higher THC cannabis cultivars. Eudesma-3,7(11)-diene and α-guaiol consistently appeared as highlighted compounds, suggesting their potential to distinguish between higher CBD and THC cannabis cultivars. ROC curve analysis revealed approximately 94% predictive accuracy for these putative markers. Given the current focus on VOCs as sensor markers for plant health, growth, and quality, the identified VOC markers─applicable across varieties and growth stages─could enable nondestructive, rapid, and accurate identification of CBD- and THC-rich cannabis species in field conditions.

Effects of ensemble-forecasted key environmental factors on the distribution, active constituents, and transcription regulation in Ligusticum chuanxiong Hort.

Ligusticum chuanxiong Hort., with over 2000 years of medicinal use and cultivation history, is extensively used in clinical settings for treating heart disease, headache, dysmenorrhea, and amenorrhea. Constructing the geographic distribution pattern of L. chuanxiong and identifying the environmental factors limiting its range, as well as clarifying the effects of key environmental factors on the content of major active constituents and transcription regulation, could provide a scientific foundation for the conservation and effective management of this valuable medicinal resource. The results reveal that the predominant environmental factors influencing the distribution were the minimum temperature of the coldest month (Bio6) and solar radiation (Srad), with cumulative account for 87.46% of the importance. Correlation analysis further reveals significant negative correlations between Bio6 and the content of major active constituents in L. chuanxiong, with Srad exhibiting a negative correlation with these constituents. The gene differential expression analysis indicated that the expression levels of some genes associated with growth and active constituent biosynthesis pathways, such as RPT2_13888, UVR8_16871, CLPB3_3155, and 4CLL5_116, varied significantly among locations influenced by differing key environmental factors. Consequently, alterations in the environment were found to influence the gene expression levels within these pathways, resulting in variations in the content of active constituents. These findings contribute to an enhanced understanding of how environmental factors impact the distribution and quality of medicinal plants and offer a theoretical reference for the introduction, cultivation, quality improvement, resource utilization and management of L. chuanxiong. © 2024 Society of Chemical Industry.

Morphophysiological, biochemical, and nutrient response of spinach (Spinacia oleracea L.) by foliar CeO2 nanoparticles under elevated CO2

Nanomaterials offer considerable benefits in improving plant growth and nutritional status owing to their inherent stability, and efficiency in essential nutrient absorption and delivery. Cerium oxide nanoparticles (CeO2 NPs) at optimum concentration could significantly influence plant morpho-physiology and nutritional status. However, it remains unclear how elevated CO2 and CeO2 NPs interactively affect plant growth and quality. Accordingly, the ultimate goal was to reveal whether CeO2 NPs could alter the impact of elevated CO2 on the nutrient composition of spinach. For this purpose, spinach plant morpho-physiological, biochemical traits, and nutritional contents were evaluated. Spinach was exposed to different foliar concentrations of CeO2 NPs (0, 25, 50, 100 mg/L) in open-top chambers (400 and 600 CO2 μmol/mol). Results showed that elevated CO2 enhanced spinach growth by increasing photosynthetic pigments, as evidenced by a higher photosynthetic rate (Pn). However, the maximum growth and photosynthetic pigments were observed at the highest concentration of CeO2 NPs (100 mg/L) under elevated CO2. Elevated CO2 resulted in a decreased stomatal conductance (gs) and transpiration rate (Tr), whereas CeO2 NPs enhanced these parameters. No significant changes were observed in any of the measured biochemical parameters due to increased levels of CO2. However, an increase in antioxidant enzymes, particularly in catalase (CAT; 14.37%) and ascorbate peroxidase (APX; 10.66%) activities, was observed in high CeO2 NPs (100 mg/L) treatment under elevated CO2 levels. Regarding plant nutrient content, elevated CO2 significantly decreases spinach roots and leaves macro and micronutrients as compared to ambient CO2 levels. CeO2 NPs, in a dose-dependent manner, with the highest increase observed in 100 mg/L CeO2 NPs treatment and increased roots and shoots magnesium (211.62–215.49%), iron (256.68–322.77%), zinc (225.89–181.49%), copper (21.99–138.09%), potassium (121.46–138.89%), calcium (118.22–91.32%), manganese (133.15–195.02%) under elevated CO2. Overall, CeO2 NPs improved spinach growth and biomass and reverted the adverse effects of elevated CO2 on its nutritional quality. These findings indicated that CeO2 NPs could be used as an effective approach to increase vegetable growth and nutritional values to ensure food security under future climatic conditions.

Preharvest sodium selenite treatments affect the growth and enhance nutritional quality of purple leaf mustard with abundant anthocyanin

Both selenium (Se) and anthocyanins are crucial for maintaining human health. Preharvest Se treatments could promote anthocyanin biosynthesis and augment Se levels in vegetables, helping to combat Se deficiencies in dietary intake. However, it remains unknown whether preharvest Se treatment could balance growth and anthocyanin biosynthesis in plants and what the appropriate treatment concentration is. In this study, preharvest treatments with sodium selenite at varying concentrations (0, 5, 10, and 30 mg/kg) affect the growth and nutritional quality of purple leaf mustard (Brassica juncea) with abundant anthocyanins. Lower Se concentrations (≤10 mg/kg) of preharvest treatments enhanced photosynthesis, facilitated root system development, consequently elevated the biomass. Conversely, higher Se levels (≥30 mg/kg) reduced photosynthesis and biomass. The dosage-dependent effects of Se treatments were corroborated through seedlings cultivated in hydroponic conditions. Moreover, nearly all Se treatments elevated the contents of various nutrients in leaf mustard, particularly anthocyanin and organic se. These results suggest an overall enhancement in nutritional quality of leaf mustard plants. Furthermore, the application of 10 mg/kg Se significantly enhanced the activity of phenylalanine ammonia-lyase and upregulated the expression of 12 genes pivotal for anthocyanin biosynthesis, further demonstrating the fortified effects of Se enrichment on anthocyanins in leaf mustard. Low-level Se treatments resulted in heightened antioxidant activity (APX, CAT, and POD), mitigating reactive oxygen species induced by increasing Se content in tissues. The enhanced antioxidant activities may be beneficial for the normal growth of leaf mustard under Se stress conditions. In conclusion, our study demonstrated preharvest Se treatment at 10 mg/kg could balance the growth and anthocyanin biosynthesis in purple leaf mustard. This study offers valuable insights into anthocyanin fortification through Se enrichment methods in agricultural practices, ensuring that such fortification does not compromise leafy vegetable yield.

Advancements in Bacteriophages for the Fire Blight Pathogen Erwinia amylovora.

Erwinia amylovora, the causative agent of fire blight, causes significant economic losses for farmers worldwide by inflicting severe damage to the production and quality of plants in the Rosaceae family. Historically, fire blight control has primarily relied on the application of copper compounds and antibiotics, such as streptomycin. However, the emergence of antibiotic-resistant strains and growing environmental concerns have highlighted the need for alternative control methods. Recently, there has been a growing interest in adopting bacteriophages (phages) as a biological control strategy. Phages have demonstrated efficacy against the bacterial plant pathogen E. amylovora, including strains that have developed antibiotic resistance. The advantages of phage therapy includes its minimal impact on microbial community equilibrium, the lack of a detrimental impact on plants and beneficial microorganisms, and its capacity to eradicate drug-resistant bacteria. This review addresses recent advances in the isolation and characterization of E. amylovora phages, including their morphology, host range, lysis exertion, genomic characterization, and lysis mechanisms. Furthermore, this review evaluates the environmental tolerance of E. amylovora phages. Despite their potential, E. amylovora phages face certain challenges in practical applications, including stability issues and the risk of lysogenic conversion. This comprehensive review examines the latest developments in the application of phages for controlling fire blight and highlights the potential of E. amylovora phages in plant protection strategies.

The use of seaweed in the production of ornamental plants

Ornamental plants play a crucial role in urban green spaces by enhancing aesthetic value and providing ecological and economic benefits. They help improve air quality, support biodiversity, and contribute to environmental purification. The horticultural industry benefits from cultivating ornamental plants, it faces challenges such as soil health and environmental pollution due to high-yielding cultivars and chemical fertilizers. To address these challenges, integrating sustainable practices, such as using seaweed as an organic fertilizer, can provide solutions. Seaweed, rich in essential nutrients and growth hormones, improves soil fertility, plant growth, and resilience against environmental stresses. Studies have shown its effectiveness in enhancing growth parameters, mitigating salinity stress, and increasing yield and quality in various ornamental plants. However, the response to seaweed varies across species and conditions. To optimize its use, tailored application rates, combined sustainable practices, and continuous monitoring are recommended. Further research is also needed to develop comprehensive guidelines for its application in ornamental plant production.

Effects of shading on morphology, photosynthesis characteristics, and yield of different shade-tolerant peanut varieties at the flowering stage.

In maize and peanut intercropping, shading emerges as a critical factor for restricting peanut growth, yield, and quality. This study investigated the impact of 30% shade on shade-tolerant [Huayu 22 (HY22) and Fuhua 12 (FH12)] and shade-sensitive [Nonghua 11 (NH11) and Nonghua 5(NH5)] peanut varieties, with non-shaded condition as the control (CK). The effects of shade stress on plant morphology, photosynthetic characteristics, dry-matter accumulation, chloroplast ultra-microstructure, yield, and quality of different shade-tolerant peanut varieties were examined. Compared to that in the control, shade stress led to an elongation of the main stem, shortening of the lateral branches, and reduction in the leaf area. However, these changes were less significant in the shade-tolerant than in the shade-sensitive peanut varieties, with minimal effect on the elongation of the main stem height and shortening of the lateral branches. Differences in leaf area became significant during the later stages of shade stress, particularly pronounced in the shade-sensitive peanut varieties. To enhance light capture by leaves, the shade-tolerant peanut varieties exhibited increased chlorophyll content and chloroplast grain-layer numbers. The decrease in the chlorophyll a/b ratio was more pronounced in the shade-tolerant than in the shade-sensitive peanut varieties, with significant differences. However, reduced activities of ribulose 1,5-biphosphate (RuBP) carboxylase/oxygenase and fructose 1,6-biphosphate aldolase (FBA) resulted in decreased net photosynthetic rates, particularly evident in the shade-sensitive peanut varieties during the late shade period. Shade stress led to decreased dry-matter accumulation, reduced weight of 100 fruits and kernels, and a significant decline in yield in the shade-sensitive cultivars. Shading also affected peanut-kernel quality. Compared with that in the control, the protein content increased and amino-acid (except cysteine) content decreased in the shade-tolerant cultivars. Under shade stress, shade tolerant peanut varieties have increased the yield by improving the photosynthetic efficiency, which provided a reference for rational selection of shade tolerant peanut varieties in maize and peanut intercropping system.

Exploring genetic variability in fodder quality of forage sorghum in the North – Eastern regions of India

This study investigated genetic variability, character association, and genetic diversity in ninety-five forage sorghum genotypes, evaluated using a randomized block design for seven fodder quality traits. Analysis of variance revealed significant differences among genotypes for all traits. Genotypes such as G90 (424B), G23 (334B), G87 (CSV21F), G76 (330B), and G45 (373B) exhibit desirable fodder quality traits. Notably, G90 (424B) and G47 (NSS5B) stand out for their superior green forage yield per plant and fodder quality, making them prime candidates for varietal development programs. Crude protein emerged as a crucial selection factor for fodder quality. Green forage yield per plant showed minimal association with the other quality traits. A strong positive correlation was observed among in vitro organic matter digestibility, metabolic energy, crude protein, and ash content. D2 analysis identified five clusters, with genotypes from clusters V and IV recommended for crossing to produce superior transgressive segregants for fodder quality. Acid detergent fibre, crude protein, and ash content significantly contributed to genetic divergence. Considering these selected traits and genotypes, they could be invaluable in future sorghum forage breeding programs aimed at enhancing fodder quality traits. Keywords: Correlation, genetic diversity, Path analysis, Sorghum Fodder quality

Molecular regulation by H2S of antioxidant and glucose metabolism in cold-sensitive Capsicum

BackgroundCold is an important environmental limiting factor affecting plant yield and quality. Capsicum (chili pepper), a tropical and subtropical vegetable crop, is extremely sensitive to cold. Although H2S is an important signaling regulator in the responses of plant growth and development to abiotic stress, few studies have examined its effects on cold-sensitive capsicum varieties. Through biotechnology methods to enhance the cold resistance of peppers, to provide some reference for pepper breeding, investigated molecular regulation by H2S of responses to cold stress in cold-sensitive capsicum plants, via physiological and transcriptomic analyses.ResultsIn capsicum seedlings, exogenous H2S enhanced relative electrical conductivity (REC) and levels of malondialdehyde (MDA) under cold stress, maintained membrane integrity, increased the activity of enzymatic and non-enzymatic antioxidants, balanced reactive oxygen species levels (O2·− and H2O2), and improved photosynthesis, mitigating the damage caused by cold. In addition, 416 differentially expressed genes (DEGs) were involved in the response to cold stress after H2S treatment. These DEGs were mainly enriched in the ascorbate–glutathione and starch–sucrose metabolic pathways and plant hormone signal-transduction pathways. Exogenous H2S altered the expression of key enzyme-encoding genes such as GST, APX, and MDHAR in the ascorbate–glutathione metabolism pathway, as well as that of regulatory genes for stimulatory hormones (auxin, cytokinins, and gibberellins) and inhibitory hormones (including jasmonate and salicylic acid) in the plant hormone signal-transduction pathway, helping to maintain the energy supply and intracellular metabolic stability under cold stress.ConclusionsThese findings reveal that exogenous H2S improves cold tolerance in cold-sensitive capsicum plants, elucidating the molecular mechanisms underlying its responses to cold stress. This study provides a theoretical basis for exploring and improving cold tolerance in capsicum plants.

Biocontrol of Phenacoccus solenopsis Tinsley using entomopathogenic fungi and bacteria

IntroductionPhenacoccus solenopsis Tinsley, poses a significant threat to a range of crops worldwide. This study aimed to evaluate the efficacy of entomopathogenic fungi (Alternaria murispora and Alternaria destruens) and bacteria (Streptomyces bellus-E23-2) against adult females of P. solenopsis under laboratory (26 ± 2°C) and greenhouse conditions.MethodsLaboratory trials tested A. murispora, A. destruens (104–1010 conidia mL−1), and S. bellus E23-2 (104–1010 cfu mL−1), alone and in combination, recording mortality rates and LC50 values. Greenhouse trials tested the best lab treatments on infested potato plants, monitoring pest density and plant quality.Results and discussionIn laboratory trials, A. murispora at 1010 conidia mL−1 was the most effective, achieved 79.7% mortality (LC50 = 1.338 × 108 conidia mL−1 after 14 days). Combination treatments significantly enhanced efficacy, with A. murispora + S. bellus E23-2 (1010 conidia mL−1 + 1010 cfu mL−1) reaching 85.3% mortality. In greenhouse trials, the combination treatments notably reduced P. solenopsis densities and increased the number of infected mealybugs, with A. murispora + S. bellus E23-2 being the most effective. These treatments did not harm plant quality, unlike imidacloprid, which reduced visual quality despite its high efficacy. Alternaria murispora and S. bellus E23-2 effectively control P. solenopsis, providing a sustainable, plant-safe alternative to chemical insecticides.

Global climate change promotes aboveground growth and high-latitude species distribution in medicinal plants: Insights from the genus Panax

Global climate change (GCC) significantly affects the quality and distribution of medicinal plants, with varying impacts across genera, posing a threat to human health. However, an understanding of such influences is unknown. Here, we take the genus Panax as a case study, using meta-analysis and MaxEnt to analyze 710 observation pairs and 588 locations, in order to determine the impact of GCC on the quality and distribution of medicinal plants. Meta-analysis indicates that GCC enhances photosynthesis and physiology, promoting aboveground growth but hindering underground development. Saponin levels in aboveground parts decline without affecting those below ground, correlating closely with biomass. Further examination revealed that the genus Panax's response to GCC is significantly moderated by various factors. MaxEnt results showed a modest range expansion, particularly beneficial for Panax ginseng (Pg) and Panax quinquefolius (Pq) at higher latitudes, but detrimental for Panax notoginseng (Pn) at lower latitudes. Notably, root rot severity peaks in China's principal Panax cultivation areas under the SSP245 scenario. Ultimately, our results propose strategies for agronomic management: "Environment matching species" by adjusting water and nitrogen inputs, and "Species matching environment" by promoting carbon reduction and optimized production for migration to higher latitudes. By integrating meta-analysis with MaxEnt modeling, we provide a novel methodological framework for future research on medicinal plant adaptation to GCC.

Evolution and functional divergence of glycosyltransferase genes shaped the quality and cold tolerance of tea plants.

Plant glycosyltransferases (UGTs) play a key role in plant growth and metabolism. Here, we examined the evolutionary landscape among UGTs in 28 fully sequenced species from early algae to angiosperms. Our findings revealed a distinctive expansion and contraction of UGTs in the G and H groups in tea (Camellia sinensis), respectively. Whole-genome duplication and tandem duplication events jointly drove the massive expansion of UGTs, and the interplay of natural and artificial selection has resulted in marked functional divergence within the G group of the sinensis-type tea population. In Cluster II of group G, differences in substrate selection (e.g., Abscisic Acid) of the enzymes encoded by UGT genes led to their functional diversification, and these genes influence tolerance to abiotic stresses such as low temperature and drought via different modes of positive and negative regulation, respectively. UGTs in Cluster III of the G group have diverse aroma substrate preferences, which contributes a diverse aroma spectrum of the sinensis-type tea population. All Cluster III genes respond to low-temperature stress, whereas UGTs within Cluster III-1, shaped by artificial selection, are unresponsive to drought. This suggests that artificial selection of tea plants focused on improving quality and cold tolerance as primary targets.

Assessing biochar, clinoptilolite zeolite and zeo-char loaded nano-nitrogen for boosting growth performance and biochemical ingredients of peace lily (Spathiphyllum Wallisii) plant under water shortage

BackgroundPeace lily (Spathiphyllum wallisii Regel) is an ornamental indoor plant with promising cut flower market, as well as antiviral, pharmacological and ecological potentials. Water deficiency can have sound effects on the growth performance and aesthetic quality of such plant. The aim of this study was to investigate the consequences of zeolite, biochar, and zeo-char loaded nano-nitrogen application on the growth performance and biochemical components of peace lily under water shortage conditions. An experiment was conducted over two consecutive seasons (2021–2022) at the experimental nursery of Ornamental Horticulture Department, Faculty of Agriculture, Cairo University, Giza, Egypt. Soil amendments; zeolite, biochar, and zeo-char loaded nano-nitrogen were prepared and applied to soil before cultivation.ResultsOur results revealed that the new combination treatment (zeo-char loaded nano-N) had an exceeding significant effect on most of the studied parameters. Vegetative traits such as plant height (35.7 and 35.9%), leaf number per plant (73.3 and 52.6%), leaf area (40.2 and 36.4%), stem diameter (28.7 and 27.1%), root number (100 and 43.5%) and length (105.7 and 101.9%) per plant, and fresh weight of leaves (23.2 and 21.6%) were significantly higher than control (commercially recommended dose of NPK) with the application of zeo-char loaded nano-N during the two growing seasons, respectively. Similar significant increments were obtained for some macro- (N, P, K, Mg, Ca) and micro- (Fe, Zn, Mn) elements with the same treatment relative to control. Chlorophyll (18.4%) and total carotenoids (82.9 and 32.6%), total carbohydrates (53.3 and 37.4%), phenolics (54.4 and 86.9%), flavonoids (31.7% and 41.8%) and tannins (69.2 and 50%), in addition to the phytohormone gibberellic acid (GA3) followed the same trend with the application of zeo-char loaded nano-N, increasing significantly over control. Leaf histological parameters and anatomical structure were enhanced with the new combination treatment in comparison with control. Antioxidant enzymes (catalase and peroxidase), proline and abscisic acid (ABA) exhibited significant declines with zeo-char loaded nano-N treatment relative to control.ConclusionThese findings suggest that incorporating soil amendments with nano- nutrients could provide a promising approach towards improving growth performance and quality of ornamental, medicinal and aromatic species under water deficiency conditions.

Insights into the uptake, accumulation, and metabolism of UV-328 in lettuce (Lactuca sativa L.) and radish (Raphanus sativus L.)

2-(2H-benzotriazole-2yl)-4,6-di-t-pentylphenol (UV-328), a newly listed persistent organic pollutant (POP) under the Stockholm Convention, has garnered significant attention. It is imperative to understand the uptake, translocation and metabolic pathways of UV-328 in plants to assess its bioaccumulation capacity and potential human health risks. In this study, the absorption, accumulation, and metabolic characteristics of UV-328 in lettuce (Lactuca sativa L.) and radish (Raphanus sativus L.) were assessed by hydroponic experiments. In the hydroponics experiment, UV-328 was significantly absorbed by the roots of the plants, with average root concentration factors (RCFs) ranging from 58.5 to 400 mL/g for lettuce and 84.4 to 154 mL/g for radish. However, UV-328 was poorly translocated from roots to shoots, with a translocation factor (TF) below 0.055. Furthermore, UV-328 underwent transformation and metabolism within the plant. Utilizing a nontarget screening strategy, fourteen phase I metabolites of UV-328 were firstly identified. The metabolic pathways of UV-328 in plants including hydroxylation, demethylation, oxidation, acetylation and deoxygenation were also suggested. It was worth noting that UV-328 has a significant adverse impact on plant growth and quality. The concentration of chlorophyll in plants exposed to UV-328 was significantly reduced, as were the concentrations of water, flavonoids, vitamin C and amino acids.