Flower Quality Research Articles

Transcriptome Sequencing Provides Insights into High-Temperature-Induced Leaf Senescence in Herbaceous Peony

Global warming causes frequent high temperatures in summer; which negatively impacts herbaceous peonies (Paeonia lactiflora Pall.) by accelerating leaf senescence and reducing biomass accumulation, leading to reduced flower quality in the subsequent year. Our findings revealed that as heat stress progressed, the high-temperature-sensitive cultivar ‘Meigui Zi’ (MGZ) exhibited a higher rate of chlorophyll content reduction and more pronounced premature aging symptoms than the high-temperature-tolerant cultivar ‘Chi Fen’ (CF). To investigate gene expression differences between CF and MGZ under high-temperature stress, we combined PacBio Iso-Seq sequencing (Iso-Seq) with next-generation sequencing (RNA-seq). Iso-seq yielded 352,891 full-length transcripts ranging from 61 bp to 49,022 bp in length. RNA-seq generated 257,562 transcripts across all samples. Further analysis revealed that differentially expressed genes (DEGs) between CF and MGZ were primarily enriched in “Photosynthesis”, with most photosynthesis-related DEGs highly expressed in CF. This indicates that CF has higher stability in its photosystem compared with MGZ, which is crucial for mitigating leaf senescence caused by high temperatures. Additionally, the highly expressed chlorophyll degradation genes stay-green (SGR) and stay-green-like (SGRL) in MGZ may be involved in chlorophyll content reduction induced by high temperature. This study preliminarily revealed the molecular mechanism of high-temperature-induced leaf senescence of in herbaceous peony and provided candidate genes for further studies of the regulation mechanism of high -temperature-induced leaf senescence.

Silica nanoparticles improve growth, chemical bioactive, and antioxidant enzyme activity of Dianthus caryophyllus L., plant

Background Nanotechnology has provided innovative use of various materials with unique properties. These substances may react positively in plants, and the induction of bioactive compounds and antioxidant activity is particularly remarkable. Ornamental plant production is a positive economic activity of great interest, especially Carnation (Dianthus caryophyllus L.). This plant is more attractive for its various colors, but the flower quality and its longevity can diminish very fast. Thus, examining methods capable of improving flower quality and vase life is crucial. Additionally, carnation can be used to treat coronary and nervous disorders. Carnation contains compounds that calm the nervous system and minimize swelling and inflammation. Objective This research aimed to evaluate the effect of silica nanoparticles (SiO2 NPs) on the growth, bioactive chemical analysis, enzyme activity, and antioxidant accumulation. Material and methods The different rates of SiO2 NPs (0, 150, and 300 ppm) were used in two ways: foliar spray and soil drench; it was applied 3 times. The first application was after 3 weeks of transplanting and repeated with 21-day intervals. Results and conclusion The results showed that foliar application of SiO2 NPs increased all morphological and flowering traits compared with drench and the control, higher levels of photosynthetic pigments and anthocyanin, greater total phenols, total sugars, and total free amino acids were obtained from plants treated with foliar spray 300 ppm followed than 150 ppm. In addition, the greatest levels of enzyme activity were recorded with plants treated with foliar spray of SiO2 NPs at a rate of 300 ppm, as well as improved anatomical structure of the stem and stomata properties compared with the drench method and control. Similarly, SiO2 NPs application method is more critical than their concentration. Therefore, it is recommended that foliar spraying of SiO2 NPs at 300 ppm can enhance plant growth, flowering, bioactive compounds, enzyme activity, and anatomical structure of the stem of carnation plants while adding SiO2 NPs drench at a rate of 150 ppm had rather acceptable findings.

Comparison of Iris dataset classification with Gaussian naïve Bayes and decision tree algorithms

In this study, we apply two classification algorithm methods, namely the Gaussian naïve Bayes (GNB) and the decision tree (DT) classifiers. The Gaussian naïve Bayes classifier is a probability-based classification model that predicts future probabilities based on past experiences. Whereas the decision tree classifier is based on a decision tree, a series of tests that are performed adaptively where the previous test affects the next test. Both of these methods are simulated on the Iris dataset where the dataset consists of three types of Iris: setosa, virginica, and versicolor. The data is divided into two parts, namely training and testing data, in which there are several features as information on flower characteristics. Furthermore, to evaluate the performance of the algorithms on both methods and determine the best algorithm for the dataset, we evaluate it using several metrics on the training and testing data for each method. Some of these metrics are recall, precision, F1-score, and accuracy where the higher the value, the better the algorithm's performance. The results show that the performance of the decision tree classifier algorithm is the most outperformed on the Iris dataset.

Comparison of Winter Flower Quality of Manually and Smart-Controlled Cut Rose ‘Beast’

Comparison of Winter Flower Quality of Manually and Smart-Controlled Cut Rose ‘Beast’

Nano Silver and melatonin effectively delay the senescence of cut carnation flowers under simulated vibrational stress

ABSTRACT Carnation (Dianthus caryophyllus L.) holds great significance as a cut flower, and its vase life plays a crucial role in determining its quality and market appeal. However, the extended transportation distances from the production location to consumer markets can have an unfavourable impact on the vase life and overall quality of cut flowers. The first experiment demonstrated that exposing cut carnation flowers to simulated vibrational stress (SVS) led to a decline in vase life and overall quality. Notably, the application of Nano Silver (NS; 25 and 50 μM) and melatonin (MT; 100 and 200 μM) into the holding solution proved effective in counteracting these negative effects, and extended the vase life by promoting cut flower water relations. In the second experiment, the optimal concentrations of NS and MT into the holding solution (25 and 100 μM, respectively) effectively reduced chlorophyll degradation, electrolyte leakage, and lipid peroxidation by enhancing the activity of catalase and peroxidase enzymes. As a result, it appears that the application of NS and MT in the holding solution has successfully prolonged vase life by both improving water balance and boosting antioxidant activity, while mitigating the negative effects of SVS.

Multi-Walled Carbon Nanotubes Improve the Development of Chrysanthemum × morifolium (Ramat.) Hemsl. ‘Jinba’ Inflorescences

The application of cut flower preservation technology can significantly enhance both the ornamental and economic value of fresh-cut flowers. Research on vase solutions has become a concentrated area in current studies on cut flower preservation. Multi-walled carbon nanotubes (MWCNTs), as a type of carbon nanomaterial with bactericidal and membrane-penetrating properties, can be used as a component in vase solutions. This supplementation of energy substances aims to improve antioxidant enzyme activity, thereby enhancing the postharvest quality of cut chrysanthemums. In this study, deionized water and a standard preservative solution were employed as control groups to compare the effects of MWCNTs applied at different concentrations, combined with common preservatives such as sugar and 8-hydroxyquinoline, on the postharvest flowering and preservation of Chrysanthemum × morifolium ‘Jinba’. By observing the distribution of MWCNTs in the tissues surrounding the cut and changes in water content, carbon sources, osmoregulatory substance levels, and the expression of relevant key genes, a formulation with excellent postharvest treatment effects was identified. Preliminary investigations into its action and mechanism were also conducted. The results indicated that the combined treatment with 5 mg L−1 MWCNTs, 30 g L−1 sucrose, and 0.2 g L−1 8-hydroxyquinoline effectively promoted water and sugar uptake in chrysanthemum flowers, accelerating bud opening, maintaining larger inflorescence diameter, and extending the vase life. Ultimately, this enhanced the ornamental value of cut chrysanthemums. These research findings provide theoretical and experimental foundations for the application of multi-walled carbon nanotubes as auxiliary additives to improve the ornamental quality of cut flowers.

The Effect of Natural Plant Growth Regulator Concentration of Indian Red Onions on the Growth of Euphorbia (Euphorbia geroldii) Flowers

The euphorbia flower (euphorbia geroldii), widely used by the public as an ornamental plant because of its visual beauty, has an aesthetic function and health benefits that still need to be researched. The euphorbia genus has antimicrobial, antioxidant, and antidiabetic activity, which comes from its abundant terpenoid compounds, flavonoids, and other phytochemical compounds. Due to these high benefits, optimization of cultivation is carried out by adding phytohormones, better known as Growth Regulatory Regulator (PGR), further to increase the quality and quantity of euphorbia flowers so that the economic value will also increase in the realm of the agricultural industry. This research aims to analyze the effect of the natural PGR concentration of red onions (Allium cepa L.) on the growth of euphorbia flowers, including the parameters of the number of flowers on one tree, the time the buds appear, and the duration of flower survival. The research method used was quantitative experimental using a one-factor Randomized Block Design (RBD), namely testing the effect of several variations in PGR concentration (0%, 1%, 3%, 5%, 7%, and 10%) and statistically analyzed using SPSS. The results of the research show that all variations in PGR concentration have a positive effect on all parameters, where the PGR concentration of 10% indicates the highest increase, including the average number of flowers on one tree, which is 13 flowers, the average time for buds to appear is 2.5 times in one week. The average duration of flower survival is 139.5 hours. It can be concluded that the natural PGR of red onions has a positive effect on all parameters, with the highest results shown at a concentration variation of 10%.

An analysis of conventional and modern packaging approaches for cut flowers: a review article.

Fresh-cut flowers are considered to be one of the most delicate and challenging commercial crops. It is important to take into consideration how to minimize loss during storage and transportation when preserving cut flowers. Many impinging (bad effect) forces can interact to shorten the flowers' vase life. In the flower industry, effective methods need to be developed to extend freshly cut flowers' life. Fresh-cut flowers' vase life can be shortened by a variety of interlocking causes. The flower industry must develop new techniques to extend the flowers' vase lifespan. This review provides comprehensive, up-to-date information on classical, modified atmosphere packaging (MAP), and controlled atmosphere packaging (CAP) displays. According to this review, a promising packaging technique for fresh flowers can be achieved through smart packaging. A smart package is one that incorporates new technology to increase its functionality. This combines active packaging, nanotechnology, and intelligence. This technology makes it easier to keep an eye on the environmental variables that exist around the packaged flowers to enhance their quality. This article offers a comprehensive overview of creative flower-saving packaging ideas that reduce flower losses and assist growers in handling more effectively their flower inventory. To guarantee the quality of flowers throughout the marketing chain, innovative packaging techniques and advanced packaging technologies should be adopted to understand various package performances. This will provide the consumer with cut flowers of standard quality. Furthermore, sustainable packaging is achieved with circular packaging. We can significantly reduce packaging waste's environmental impact by designing reused or recyclable packaging.

Influence of growing condition, spacing and nutrition on flower quality and post harvest life of Heliconia genotypes

Heliconia, a high value flower crop can assure sizable income to farmers with minimum investment and care. Suitable agro techniques help in obtaining maximum number of high quality flowers. Therefore, field trial was undertaken at the Department of Horticulture, University of Agricultural Sciences, Dharwad during 2021-22 to understand the influence of growing condition, spacing and nutrition to improve the flower quality and post harvest life of Heliconia genotypes. The experiment was laid out in strip plot design with three replications and sixteen treatment combinations comprised of two growing conditions as main plot (M1:Coconut garden and M2:Shade house) and combinations of genotypes, spacing and nutrition as eight sub plots [G1:Orange (H. latispatha), G2:Kenea Red (H. psittacorum), S1:40 × 40 cm, S2:40 × 60 cm, N1:100% recommended dose of fertilizer (RDF) and N2:75% RDF]. Result indicated significant difference with all flower quality parameters and post harvest life. Plants in shade house exhibited highest values for all the characters compared to plants in coconut garden. The interaction found significant for stem length (54.01 cm) which was higher under T13 (M2G2S1N1). Treatment combination T11 (M2G1S2N1) recorded maximum stem girth (2.37 cm), spike length (19.87 cm) and spike width (21.67 cm). The same treatment combination (T11:M2G1S2N1) recorded maximum fresh weight of flowers (117.68 g), lowest physiological loss in weight (23.26%) and highest vase life of 16.60 days. and the same treatment combination can be suggested to get good quality flowers with extended vase life in Heliconia.

Loss of daylength sensitivity by splice site mutation in Cannabis pseudo-response regulator.

Photoperiod insensitivity (auto-flowering) in drug-type Cannabis sativa circumvents the need for short day (SD) flowering requirements making outdoor cultivation in high latitudes possible. However, the benefits of photoperiod insensitivity are counterbalanced by low cannabinoid content and poor flower quality in auto-flowering genotypes. Despite recent studies in cannabis flowering, a mechanistic understanding of photoperiod insensitivity is still lacking. We used a combination of genome-wide association study and genetic fine-mapping to identify the genetic cause of auto-flowering in cannabis. We then used gene expression analyses and transient transformation assays to characterize flowering time control. Herein, we identify a splice site mutation within circadian clock gene PSEUDO-RESPONSE REGULATOR 37 (CsPRR37) in auto-flowering cannabis. We show that CsPRR37 represses FT expression and its circadian oscillations transition to a less repressive state during SD as compared to long days (LD). We identify several key circadian clock genes whose expression is altered in auto-flowering cannabis, particularly under non-inductive LD. Research into the pervasiveness of this mutation and others affecting flowering time will help elucidate cannabis domestication history and advance cannabis breeding toward a more sustainable outdoor cultivation system.

Novel strategies for enhancing quality stability of edible flower during processing using efficient physical fields: A review

Edible flowers are an exotic part of the human diet due to their distinct sensorial properties and health benefits. Due to consumers demand edible flowers and their products with natural freshness and high nutritional value, there is increasing research on the application of green and efficient edible flower processing technologies. This paper reviews the application of a number of physical fields including ultrasound, microwave, infrared, ultraviolet, ionizing radiation, pulse electric field, high hydrostatic pressure, and reduced pressure aiming to improve the processing and product quality of edible flowers. The mechanism of action, influencing factors, and status on application of each physical energy field are critically evaluated. In addition, the advantages and disadvantages of each of these energy fields are evaluated, and trends on their future prospects are highlighted. Future research is expected to focus on gaining greater understanding of the mechanism action of physical field-based technologies when applied to processing of edible flowers and to provide the basis for broaden the application of physical field-based technologies in industrial realm.

Epitypification of the Linnaean Statice echioides (Plumbaginaceae) and notes on Smith's concepts of S. aristata and S. rorida

AbstractThe typification of the Linnaean name Statice echioides (currently Limonium echioides) is discussed. An illustration from Magnol's Botanicum Monspeliense (“Limonium minus annuum bullatis foliis vel echioides”) was correctly designated as lectotype by Erben in 1978 according to Art. 7.11 of Shenzhen Code. However, this image lacks some of the important flower characters required for a critical identification of the species. An epitype (plate no. 299 in Sibthorp & Smith's Flora Graeca) is selected to serve as an interpretative type of the lectotype of S. echioides (Art. 9.6). In addition, the name S. aristata, published in Sibthorp & Smith's Flora Graecae prodromus, is discussed in depth and lectotypified on the above‐mentioned Magnol illustration, making this name homotypic with the Linnaean one. Finally, the concept of S. echioides, as described in Flora Graecae prodromus, is discussed, concluding that it refers to the currently recognized Limonium roridum (≡ S. rorida, as originally published in Flora Graeca).

Evaluation of the growth, flower quality, yield and vase life of gerbera (Gerbera jamesonii L.) cultivars under open ventilated saw-tooth type polyhouse

Cultivation of crops under protected conditions can enhance the yield and quality of agricultural produce. Gerbera is one of the high-value cut flowers which can be commercially cultivated under greenhouse conditions in tropical and subtropical regions. The choice of commercially viable cultivars is essential for obtaining its marketability. Moreover, cultivars of gerbera should be highly productive. Considering the above facts, the present study was conducted in 2020-2022 in the Protected Cultivation Unit of the Centurion University of Technology and Management where 10 cultivars were chosen as treatments. They were Intense, White house, Stanza, Dune, Dana-ellen, Artist, Sunway, Silvester, Pre-intenzz and Ankur. The experiment was laid out in a randomized complete block design and replicated three times. The data collected on the growth parameters, quality, yield, and vase life revealed that among all the cultivars of gerbera, Dana-ellen showed its superiority over others in terms of plant height and number of leaves (49.3 cm and 31.0 at 120 days after sowing respectively), days to first flowering (84.3), flower stalk length (64.8 cm), flower stalk girth (67.2 cm), ray floret length (8.7 mm) and suckers per plant (3.4); however, it remained on par with Dune, Intense, and Silvester in expression of most of the crop parameters. Similarly, the same variety Dana-ellen registered the highest yields (27.3, 33.7 and 34.7 flowers per plant during first, second and third years of the study respectively) among all the cultivars. Moreover, the variety Dune recorded the maximum vase life of 8.4 days and 9.2 days with citric acid and sucrose solutions, respectively. The study concluded that the cultivation of Dana-ellen, Dune, Silvester, and Artist under open-ventilated saw-tooth polyhouse conditions can be commercially viable for achieving higher flower quality and yield.

Comparison of morphological characters, flowering phenology and essential oils of two accessions of Cananga (Cananga odorata var. fruticosa)

Abstract. Na’imah MNR, Kurniawati A, Kisantini. 2024. Comparison of morphological characters, flowering phenology and essential oils of two accessions of Cananga (Cananga odorata var. fruticosa). Biodiversitas 25: 801-810. The Cananga flowers are well-known in various regions of Indonesia as a raw material for essential oils and flower decorations. This study aims to compare the characteristics of two varieties of Cananga originating from Kediri and Cipanas. Both types of Cananga were cultivated in the Institut Pertanian Bogor (IPB) Cikabayan experimental field. Observation was conducted on leaf and flower characteristics, flowering phenology, and essential oil content. The research shows that the Kediri accession exhibited brownish-red leaf petioles, brownish-red branches, and dark brown stems. In contrast, the Cipanas accession had green leaf stalks, green branches, and light brown stems. The flowering phenology of the Kediri accession spanned from the emergence of flower buds to the End stage, lasting 35 days, while the Cipanas accession's flowering period extended for 25 days. The Kediri accession's flowers have more petals and are longer and heavier than the Cipanas accession. The highest essential oil content of the Cipanas accession occurred at the 50% full flowering stage, reaching 0.92%, and gradually decreased as the flowering progressed. In comparison, the Kediri accession oil content is the highest at the end of the flowering stage, with a peak of 0.61%. The highest compound in the Kediri accession is all-trans-Farnesol acetate 14,24%, ?-Copaene 12.23%, and Caryophyllene 15.92%. Meanwhile, in the Cipanas accession, the highest compound content was linalool 18.74% and caryophyllene 16.00%.

Agents of Pollination on Fruit Set of Piper nigrum L.

The role of agents on pollination and fruit set of Piper nigrum L. was carried out in the Department of Plantation Crops and Spices, College of Agriculture, Vellayani, Thiruvantha puram during 2017-2019. The role of wind, gravity, rain and dew were investigated and the data was analyzed on twenty-five inflorescences of field grown black pepper plants and twenty-five inflorescences of pot grown bush pepper plants. The fruit set was superior due to the influence of wind and gravity (95.99%) in pot grown bush pepper and it was 91.98 per cent in field grown black pepper vines. The percentage of fruit set due to wind in field grown black pepper and potted bush pepper was 77.67% and 59.39 % respectively. The role of rain water in the pollination also revealed higher fruit set of 92.76% under natural conditions. Insects which visited black pepper spike were foraging nectar and common visitors were yellow crazy ant, black garden ant and pollu beetle with visit rates of 1.79, 1.41 and 0.45 respectively. The small size of pollen with high pollen availability makes it suitable to be carried away by wind. The pendulous nature of the spike, the shedding of pollen, the pollen carried by the rainwater or dew down the spike can all contributed to pollination. Integration of more factors resulted in more fruit set. Characteristics of black pepper flowers studied revealed their adaptation for wind pollination and the pendulous nature of the spike makes it efficient to carry the pollen due to gravity as well as rain and dew for pollination. The role of insects in pollination in black pepper was precluded.

Two lineages of Lemna aequinoctialis (Araceae, Lemnoideae) based on physiology, morphology, and phylogeny.

Lemna aequinoctialis Welw. is a widely spread species that has diverse physiological and molecular properties. Flower characteristics are important factors in deducing taxonomical status; however, owing to the rarity of flowering observations in Lemna, studying them has been a prolonged challenge. In this study, physiological and morphological analyses were conducted by inducing flowering, and molecular analysis was done based on the two chloroplast DNA loci (matK, atpF-atpH intergeneric spacer) of L. aequinoctialis sensu Landolt (1986) from 70 strains found in 70 localities in Japan, Korea, Thailand, and the US. In total, 752 flowering fronds from 13 strains were observed based on axenic conditions. Two different trends in flower organ development-protogyny and adichogamy-were detected in these strains. Their physiological traits were divided into two groups, showing different morphological features based on frond thickness, root cap, and anther sizes. Molecular analysis showed two lineages corresponding to two physiological groups. These were identified as L. aequinoctialis sensu Beppu et al. (1985)and L.aoukikusa Beppu et Murata based on the description of the nomenclature of L. aoukikusa. These were concluded as independent taxa and can be treated as different species. Furthermore, the distribution of L. aoukikusa is not only limited to Japan.

Using photographs and deep neural networks to understand flowering phenology and diversity in mountain meadows

AbstractMountain meadows are an essential part of the alpine–subalpine ecosystem; they provide ecosystem services like pollination and are home to diverse plant communities. Changes in climate affect meadow ecology on multiple levels, for example, by altering growing season dynamics. Tracking the effects of climate change on meadow diversity through the impacts on individual species and overall growing season dynamics is critical to conservation efforts. Here, we explore how to combine crowd‐sourced camera images with machine learning to quantify flowering species richness across a range of elevations in alpine meadows located in Mt. Rainier National Park, Washington, USA. We employed three machine‐learning techniques (Mask R‐CNN, RetinaNet and YOLOv5) to detect wildflower species in images taken during two flowering seasons. We demonstrate that deep learning techniques can detect multiple species, providing information on flowering richness in photographed meadows. The results indicate higher richness just above the tree line for most of the species, which is comparable with patterns found using field studies. We found that the two‐stage detector Mask R‐CNN was more accurate than single‐stage detectors like RetinaNet and YOLO, with the Mask R‐CNN network performing best overall with mean average precision (mAP) of 0.67 followed by RetinaNet (0.5) and YOLO (0.4). We found that across the methods using anchor box variations in multiples of 16 led to enhanced accuracy. We also show that detection is possible even when pictures are interspersed with complex backgrounds and are not in focus. We found differential detection rates depending on species abundance, with additional challenges related to similarity in flower characteristics, labeling errors and occlusion issues. Despite these potential biases and limitations in capturing flowering abundance and location‐specific quantification, accuracy was notable considering the complexity of flower types and picture angles in this dataset. We, therefore, expect that this approach can be used to address many ecological questions that benefit from automated flower detection, including studies of flowering phenology and floral resources, and that this approach can, therefore, complement a wide range of ecological approaches (e.g., field observations, experiments, community science, etc.). In all, our study suggests that ecological metrics like floral richness can be efficiently monitored by combining machine learning with easily accessible publicly curated datasets (e.g., Flickr, iNaturalist).

Bio- and synthetic fertilizers for reducing root rot and wilt and improving growth and flowering characteristics of rose

Root rot and wilt diseases are among the most pressing obstacles to the production of rose flowers in Egypt. Isolation results showed that these diseases are mainly caused by seven soil-borne fungi. However, Fusarium roseum, Verticillium dahlia, and Rhizoctonia solani were the most pathogenic fungi against Rosa gallica and R. chinensis compared to other isolated fungi. The present study aimed to investigate the potential of some bio- and synthetic fertilizers, including seaweed extract, Rhizobacterin, NPK, and potassium silicate, as well as the chemical fungicide vitavax 200, to control root rot and wilt and improve growth and flowering traits of both R. gallica and R. chinensis. Under in vitro conditions, the highest linear growth inhibition of pathogenic fungi was achieved by seaweed extract followed by potassium silicate, at 400 ppm each. In addition, vitavax 200 at 400 ppm completely inhibited the linear growth of these fungi. The results also showed that all treatments applied in vivo significantly reduced the incidence of diseases on rose plants in both seasons, leading to an improvement in all growth and flowering parameters and an increase in the content of photosynthetic pigments, total carbohydrates, nitrogen, phosphorus, and potassium. In this regard, seaweed extract (4 and 2 g/L) and potassium silicate (4 g/L) were the most efficient, while Rhizobacterin (2 g/L) and NPK (2 g/plant) were the least effective. However, vitavax 200 was the most effective of all the treatments used. In conclusion, the results proved the possibility of increasing the tolerance of R. gallica and R. chinensis against root rot and wilt, while improving growth and flowering characteristics by using some bio- and synthetic fertilizers.

Two Growing-Season Warming Partly Promoted Growth but Decreased Reproduction and Ornamental Value of Impatiens oxyanthera.

Climate warming profoundly affects the vegetative growth, flowering phenology and sexual reproduction of plants; therefore, it affects the ornamental value of wild flowers. Despite this, the extent and mechanism of the impact remain unclear. Here, we conducted a warming experiment for two growing seasons (increases of 1.89 °C in 2017 and 2.37 °C in 2018) with infrared heaters to examine the effects of warming on the ornamental value of the wild flower Impatiens oxyanthera, endemic to China, in Mount Emei. We evaluated the comprehensive ornamental value based on plant morphology and flowering characteristics using the analytic hierarchy process (AHP) and disentangled the impact of the two traits on ornamental value using principal component analysis (PCA) and the partial least squares structural equation model (PLS-SEM) under ambient and warming treatments. We hypothesized that warming would reduce the ornamental value of I. oxyanthera in terms of plant morphology and flowering traits. Our results showed that warming significantly decreased plant height and crown width and increased branch number and single-leaf area. Warming also decreased vexillum length, corolla tube length, nectar spur length and pedicel length. In addition, warming shortened flowering duration per plant and reduced flower number, while there was no significant effect on flower longevity and flower color at full-bloom stage between the control and warming treatment. Therefore, the comprehensive ornamental value under warming was lower than that under the control. Pedicel length, flower color, flower longevity and flowering duration per plant were the main factors affecting the comprehensive ornamental value. The PLS-SEM showed that warming had an indirect negative effect on ornamental value via direct negative effects on flowering traits. Collectively, these results indicate that, although promoting vegetative growth, short-term warming significantly decreased the ornamental value of I. oxyanthera due to warming-caused smaller flowers and shorter flowering duration.

Effect of Micro Climate on Flowering Characteristics in Grapes (Vitis vinifera L.)

The study aimed to find out the effect of micro climate on flowering characteristics in grapes varieties viz. Perlette, Flame Seedless, Superior Seedless and Punjab Purple. The experiment was conducted in Punjab-Ludhiana during 2018-19 fruiting seasons. Grape varieties were grown in both protective covering (Greenhouse) and open field. The data recorded showed that temperature and relative humidity was higher in protective covering with comparison to open field. The result of the experiment showed advancement in flowering characteristics viz. time of bud-burst, flowering initiation and fruit set in grape varieties grown under protective covering as compared to those grown in open field condition. Maximum advancement in bud burst was observed in Perlette variety (9 and 5 days earlier). Flower initiation was advanced by 4 days in Flame Seedless and Superior Seedless varieties and fruit set by 3-10 days in Punjab Purple variety grown in protective covering.