The present investigation was conducted during Kharif 2017-2018 at the Field Experimentation Centre of the Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology & Sciences (U.P), during the year of 2017-2018. The experiment was conducted in field and laboratory to evaluate of organic and inorganic fertilizer on mother plant. The experimental trial consists of 8 treatment combinations comprising of 2 different varieties of chilli carried out in Randomized Block Design (factorial). The chilli varieties used were Suryamukhi and Chilli G-4. The treatments included T0= control, T1= Urea RDN, T2= 100% Vermicompost, T3= 50 % Urea and 50 % Vermicompost, T4 = 100% FYM, T5= 50% Urea and 50% FYM, T6 = 50 % Vermicompost and 50% FYM and T7 = % 50 Urea, 50 % Vermicompost and 50 % FYM. All the 9 seed quality parameters treated with T7 recorded higher are germination per cent (99 %), root length (2.37 cm), shoot length (3.06 cm), seedling length (5.37 cm), seedling dry weight (0.036 mg), vigour index I (531.95), vigour index II (3.60), speed of germination (2.155), germination energy (2.940), and lower electrical conductivity (1.116). The application of treatment with T7 (% 50 Urea, 50 % Vermicompost and 50 % FYM) dose in mother plant. All the fertilizer treatment except chemical fertilizer shows better performance in all the characters. The variety G-4 performed better than Suryamukhi.