Bread Quality Research Articles

Addition of micronized oat husk fiber to gluten-free bread - Effects on chemical, physical and physiolgical properties

Gluten-free (GF) breads available on the market are still mainly produced from refined flours or starch, thus they lack nutritional quality, like dietary fiber, minerals and vitamins. Incorporation of micronized oat husk (MOH) could improve these properties. MOH is a dietary fiber and a food by-product, rich in polyphenols exhibiting antioxidant properties. This study investigated the addition of 0–20% MOH (in 5% steps) to GF breads baked conventionally (deck oven) and by ohmic heating (OH). Incorporation of ≤20% MOH had only a slight effect on the bread texture properties (specific volume, pore properties, crumb firmness and relative elasticity), and was less pronounced for OH baked breads. Both, the crumb and crust color, darkened proportionally to the added amount of MOH. Total phenolic content (TPC) and antioxidative properties were significantly increased by MOH addition, as well as the ability to protect lipids from oxidation. In vitro starch digestibility was improved with MOH addition for OH-baked breads. GF breads containing ≥15% MOH can be claimed as “source of fiber”, ≥20% MOH as “high in fiber”. Overall, this study demonstrated that micronized oat husks have a good potential to enhance the quality of GF breads.

Effect of commercial lipase incorporation on technological properties of bread

Additives are used to improve bread quality; for this reason, lipase stands out, as it simulates the effect of emulsifiers. The present work aimed to verify the effect of commercial lipase addition on the physical and technological characteristics of the bread. The first stage used 33 factorial design namely three enzymes, five concentrations, and three fermentation times. The second stage used 23 factorial design, and stored over 28 days, with two formulations (control and with enzyme) and three storage temperatures. The PCA identified the variables with the most significance in PC1: specific volume, volume, and variables related to texture and expansion. In the second stage (shelf life), the PCA revealed that the bread samples with enzymes in the formulation differed from the control samples. Based on the circle of correlations, enzyme samples were distinguished by lower chewiness, gumminess, and hardness. Microscopic analysis showed that the starch molecules were more uniform in the first 14 days. However, the starch molecules lost their conformational structure afterwards. Therefore, the incorporation of lipase improved the bread’s technological parameters such as volume, texture, and structure.

The combination of the kaizen approach with the Design of Experiment (DoE) method for improving quality of the bread and donut products in SMEs

Competition for Small and Medium Enterprise (SME) products is currently getting tougher, in terms of quality, timely delivery, and competitive prices. SME customers of bread and donut products sometimes complain about the visual quality of bread and donut products that are stale, burnt, and dirty. The purpose of this research is to reduce product defects during the production process and improve the quality of the bread and donut products. This research method uses a Kaizen approach combined with the Design of Experiment (DoE) one-shoot case study method and uses organoleptic tests to assess the quality of bread and donut products. This study found that there were 3 product defect problems, namely bread and donut defects that did not rise, burnt donut defects, bread defects, and dirty donuts. This research resulted in a decrease in defects of 65% from 3.84% to 1.07%. While the best DoE test results used dough type C with 10 grams fermipan/yeast composition and 15 grams bread improver, stove 165-1750C, equipment cleaning frequency 1 time for each dough, and organoleptic test with 10% beetroot extract addition resulted in a color aspect score of 70 %, 65% aroma aspect, 68.5% texture aspect, and 70% taste aspect which is liked by many people.

Bakery Packaging Improvement through Kansei Engineering: A Multi-Objective Analysis of Consumer Preferences

This study investigates the packaging improvement process for plain bread at CV. X. Consumer complaints regarding the use of only tape for sealing and the subsequent negative impact on bread quality (shape, texture, shrinkage) prompted the bakery to explore packaging redesign. This research aims to redesign product packaging using Kansei Engineering, specifically Kansei Type 1, and multivariate analysis. The initial steps of Kansei Engineering Type 1 involve collecting 40 Kansei words, refining them to 30, and then creating a questionnaire for distribution to consumers. The collected questionnaire data will undergo multivariate analysis based on respondent feedback. Desired packaging criteria include bold product branding, multi-color packaging, featuring halal and food safety logos, a prominent company logo, a box-shaped package, made from Oriented Polypropylene (OPP) plastic, and sealed with clip closure.

PRELIMINARY STUDIES CONCERNING THE INFLUENCE OF BUCKWHEAT FLOUR ON THE QUALITY OF WHITE WHEAT BREAD

Buckwheat flour is considered an important nutritious source of protein (high lysine content), polyphenols compounds with high antioxidant activity, dietary fibres, vitamins, minerals. Buckwheat flour in combination with other flours and used in bakery provide multiple health benefits (constipation, reducing cholesterol, obesity, reducing hypertension, cancer prevention, maintaining the optimal level of glycemic index). In the present paper, the effects of replacement of wheat white flour with whole buckwheat flour (0 to 12.5 %) on the sensory and physico-chemical properties of bread samples were investigated. The buckwheat flour had influenced positively the quality of white bread, the proportion of 10 % buckwheat flour showing better properties.

Survival of encapsulated Lactiplantibacillus plantarum probiotics on soft bread throughout gastrointestinal tract transit: Physicochemical characteristics, sensory profile, and functional activity

Bread is one of the most widely consumed foods in the world and is part of the regular diet in many countries. However, white flour bread lacks many valuable nutrients. Inclusion of probiotics can result on bread's quality improvement, but their viability is often affected by food processing and digestion. In this study, maltodextrin and sweet whey were used to encapsulate Lactiplantibacillus plantarum via spray drying. The effect on probiotics survival during heating, storage, and gastrointestinal tract transit, simulated by the INFOGEST in vitro digestion model, was studied. Quality characteristics, sensory analysis, and consumer acceptance were also evaluated. Spray dried bacteria survived in bread during baking at 180 °C and 15 day-storage with a final viability of 7.6 log CFU/g. Encapsulation also ensured the survival of Lactiplantibacillus plantarum during digestion (6.1 log CFU/g), allowing them to be released in the small intestine to exert their health benefits. A difference in bacterial viability between bread crust and crumb was observed, related to oxygen availability and moisture content. A sensory panel did not identify significant differences between bread produced with and without encapsulated bacteria. These results provide new insights regarding addition of encapsulated probiotics for developing functional bakery products favored by consumers.

Alkali-Induced Protein Structural, Foaming, and Air-Water Interfacial Property Changes and Quantitative Proteomic Analysis of Buckwheat Sourdough Liquor.

In this study, the protein structural, foaming, and air-water interfacial properties in dough liquor (DL) ultracentrifugated from buckwheat sourdough with different concentrations of an alkali (1.0-2.5% of sodium bicarbonate) were investigated. Results showed that the alkali led to the cross-linking of protein disulfide bonds through the oxidation of free sulfhydryl groups in DL. The alterations in protein secondary and tertiary structures revealed that the alkali caused the proteins in DL to fold, decreased the hydrophobicity, and led to a less flexible but compact structure. The alkali accelerated the diffusion of proteins and decreased the surface tension of DL. In addition, the alkali notably improved the foam stability by up to 34.08% at 2.5% concentration, mainly by increasing the net charge, reducing the bubble size, and strengthening the viscoelasticity of interfacial protein films. Quantitative proteomic analysis showed that histones and puroindolines of wheat and 13S globulin of buckwheat were closely related to the changes in the alkali-induced foaming properties. This study sheds light on the mechanism of alkali-induced improvement in gas cell stabilization and the buckwheat sourdough steamed bread quality from the aspect of the liquid lamella.

Closing the Fibre Gap-The Impact of Combination of Soluble and Insoluble Dietary Fibre on Bread Quality and Health Benefits.

Dietary fibre (DF) is important for overall health and disease prevention. However, the intake of DF in Westernised countries is below the recommended level, largely due to the excessive consumption of low-fibre foods. Fortifying staple foods, such as bread, with dietary fibre ingredients is one approach to closing the fibre gap in our diet. However, incorporating purified and chemically modified fibre ingredients into food is challenging. This study unveils interactions between soluble-fermentable (arabinoxylan), insoluble-fermentable (resistant starch type IV) and insoluble-unfermentable (cellulose) fibre ingredients and their impact on bread quality using Response Surface Methodology. This resulted in an optimised mixture of these fibre ingredients that can coexist within a bread matrix while maintaining quality characteristics comparable to white wheat bread. The partial replacement of flour with fibre ingredients led to an interference with the gluten network causing a reduction in gluten strength by 12.4% and prolonged gluten network development time by 24.4% compared to the control (no fibre addition). However, the CO2 retention coefficient during dough fermentation was not affected by fibre ingredient inclusion. The fibre content of the white bread was increased by 128%, with only a marginal negative impact on bread quality. Additionally, the fibre-fortified bread showed a lower release of reducing sugars during in vitro starch digestion. This study illustrates the synergy of different types of fibre ingredients in a bread system to advance in closing the fibre gap.

Honey bee protein and lipid nutrition in avocado and blueberry agroecosystems with conventional and organic management

Honey bees play a crucial role in agricultural production. Farmers rely on commercial bee pollination to obtain optimal yields, and beekeepers on the income from pollination fees. However, commercial pollination confronts honey bees with pests and pathogens, pesticides, and low-quality food, which in many cases do not fulfill the minimal nutritional requirements of honey bees. In this work, we evaluated honey bee nutrition in avocado and blueberry plantations under organic and conventional management, by assessing the nutritional quality of pollen and bee bread based on their protein, fatty acid, and microbial content, and by analyzing honey bee health through the examination of abdominal fatty acid profiles and head protein content. Low protein content in honey bee hemolymph was evident under conventional management. Regardless of management, avocado pollen showed higher protein levels, which were translated into higher honey bee head protein levels being 60% higher in avocado under conventional management when compared to blueberry under the same management. However, higher protein levels in avocado pollen were also translated into lower amounts of fatty acids in bee bread and in honey bee fat. In particular, the total amount of fatty acids in bee bread from conventional management was 50% lower than that from organic management. Additionally, the saturated fatty acids in conventional blueberries were lower compared to that of organic blueberries. Crop system and bee bread microbial composition altered the plant-honey bee nutrition cascades transforming pollen to bee bread with increased amount of saturated, unsaturated, and total fatty acids. In conclusion, both crop species and crop systems determine honey bee nutrition through alterations in the pollen transformation, affecting protein and fatty acid assimilation.

Enhancing bread quality and extending shelf life using dried sourdough

This study investigated the physicochemical properties, microbiological analysis, antifungal activity, and mycotoxin analysis of bread. Eight breads were prepared, including control Bread (CB), control Bread with calcium propionate (CPB), commercial sourdough breads (CCB), control bread made with oven-dried sourdough (SB), and bread baked with oven-dried sourdough fermented by Pediococcus pentosaceus TI6 (SB-TI6). These breads were contaminated with Aspergillus flavus and Penicillium verrucosum, and their shelf life was monitored over 7 days. The results indicated that incorporating sourdough powder into bread significantly extends the shelf life and reduces fungal contamination. This reduction resulted in lower levels of mycotoxins, with the use of dry sourdough fermented by P. pentosaceus TI6 leading to the lowest levels of aflatoxins. In the case of SB-TI6, minor levels of aflatoxin B1 (AFB1), B2 (AFB2), and G1(AFG1) were detected, with only 0.003 mg of AFG2/Kg of the product. Conversely, CB loaves exhibited the highest mycotoxin contamination levels, reaching values of 330 mg of AFB1/Kg. The findings suggested that incorporating dry sourdough in breadmaking served as a viable alternative to synthetic preservatives, effectively reducing mycotoxin levels and enhancing bread safety and shelf life.

Wheat bread making (WBM)-like seed proteins (WSPN): A new family of small prolamins in barley

Expression level of the gene Wheat Breadmaking (TaWBM) has been shown to be correlated with bread quality, and influences the rising of the bread loaf. We found that a family of the wbm homologous genes are present not only in wheat, but also in oat, rye and barley. However, a WBM protein has never been demonstrated experimentally. We have identified the existence of a family of WBM-like Seed Proteins (WSPN) from the prolamin fraction of barley flour, encoded by three closely linked genes on the long arm of chromosome 7H, analysing the gene expression by qPCR and relative protein levels by mass spectroscopy. All three genes are expressed specifically in seeds with no detectable expression in the leaves. The barley WSPNs share a common gene structure with a single exon containing an open reading frame in the size 253–313 bp, with the first 80 bp predicted by TargetP 2.0 to encode a sorting peptide. Furthermore, they all contain the conserved motif C–P-X-G-X4-C-X(4–8)-C-X-C. Structure prediction with Alphafold 2.0 suggest that this motif is a structurally conserved microdomain consisting of two antiparallel strands where the cysteines align.

Effect of ultra‐fine ground whole grain highland barley substitution on wheat dough properties and bread qualities

AbstractBackground and ObjectiveThere is a growing interest in incorporating whole grain highland barley (HB) to wheat bread for improving nutrients and potential health benefits. However, the qualities and sensory properties of whole grain‐based bread were reduced compared to refined wheat‐based bread due to the strong water absorption and large particle size of dietary fiber in whole grain. Therefore, ultra‐fine ground whole grain HB partially substituted 5%–40% wheat flour to make bread, and the relevant dough properties and bread qualities were studied.FindingsThe increased addition of ultra‐fine ground whole grain HB from 5% to 40% to wheat increased dough development time, whereas, decreased dough stability time, elasticity, pH, G′ modulus, and G″ modulus. The increased substitution of ultra‐fine ground whole grain HB to wheat further reduced the specific volume, cohesiveness, elasticity, and resilience of bread, whereas, increased the hardness, adhesiveness, and chewiness of bread.ConclusionsThe partial substitution of wheat with ultra‐fine ground whole grain HB from 5% to 40% wheat significantly affected wheat‐HB dough properties and resultant bread qualities mainly due to weakened gluten networking.Significance and NoveltyThe 5%–10% substitution of wheat by ultra‐fine ground whole grain HB showed the optimum dough properties and bread qualities compared to other treatments, which substituted 20%–40% wheat flour.

Contribution of Gamma-Aminobutyric Amino Acid and Free Amino Acids to Low-Salt Whole-Wheat Bread through the Addition of Spice Extracts-An Approach Based on Taste Quality.

Given the link between excessive salt consumption and hypertension, reducing salt levels in bread, an important staple food in Japan, is essential. γ-Aminobutyric acid (GABA) has a salty taste-enhancing effect in vivo, and its production is influenced by the type of spice extract in vitro. However, the effects of spices on GABA levels, total free amino acid composition, and taste quality in whole-wheat bread remain unclear. Therefore, this study aimed to investigate whether the addition of spice extracts, which do not affect bread flavor and taste, can increase the GABA level in low-salt whole-wheat bread and whether free amino acid content affects the taste quality of bread using an automatic home bread maker. Through free amino acid composition analysis and sensory testing, we evaluated the influence of six spice extracts on the composition of free amino acids, including GABA, in whole-wheat bread. We found that cumin and anise extracts were effective in increasing the GABA level to approximately twice that in whole-wheat bread. Moreover, both the preference and saltiness of the bread were favorable, indicating that these extracts are useful for reducing the salt content of whole-wheat bread. This study provides a theoretical basis for guiding industrial production.

Application and safety of pectin substances from vegetable raw materials in bread production

Currently, pectin substances and their compounds are often used in cooking as an anionic surfactant. In the technology of food preparation, such properties of pectin substances as swelling, thickening, gel-forming, crystalforming, water-absorbing ability, and emulsifying ability are of great importance. Like any gel-forming fiber, pectin helps remove heavy metals from the body such as lead, mercury, cadmium, etc. MCP may be better suited for these purposes, as it has been used in four clinical studies of heavy metal detoxification. Considering the accumulation of radioactive elements, heavy metal salts and pesticides in the environment and the human body, Jerusalem artichoke, which is rich in pectin substances, carotene and dietary fiber, is of particular interest. It is also economically beneficial as it is a domestic raw material. The goal of scientific research is to obtain a new type of functional bread with the addition of vegetable pectin. In this article, it was determined that the addition of pectin to dough has an effect on biochemical, colloidal, as well as microbiological processes. It also affects the physicochemical and rheological properties and reduces the content of contaminants. The work investigated that when pectin substances are added to the dough, its initial acidity increases. Acidity varied 2.2-2.4. It was also noted that in the test sample the fermentation process was more active; the activity of the fermentation process was associated with the addition of sugar along with pectin. As a result of this work, in order to improve the quality of bread and extend shelf life, pectin from raw materials of plant origin was added, the properties of bread samples with Jerusalem artichoke pectin with high performance were studied, and their organoleptic, physicochemical and safety indicators were studied. It was revealed that pectin reduces the content of some toxic elements by up to 100%. Practical significance: the conducted studies showed that the obtained samples of functional bread can be recommended for introduction into production to expand the range of bakery products.

Unraveling chemical changes associated with the sensory quality of Chinese steamed bread as altered by wheat flour type

Chinese steamed bread (CSB) is an important staple of the Chinese people, and its flavor profile is mostly affected by wheat varieties among others. This study selected wheat flour made from three different wheat varieties and investigated their contribution to the CSB flavor profile in terms of metabolism. Thirteen aroma-active compounds identified by GC-O were determined as the main contributors to the different aroma profiles of three CSBs. 350 sensory trait-related metabolites were obtained from five key modules via weighted gene co-expression network analysis. It was found that the sensory characteristics of CSBs made of different wheat flour were significantly different. The higher abundance of lipids in Yongliang No.4 (YL04) wheat flour was converted to large number of fatty acids in fermented dough, which led to the bitterness of CSB. Besides, the abundance in organic acids and fatty acids contributed to the sour, milky, wetness and roughness attributes of YL04-CSB. More fatty amides and flavonoids in Jiangsu Red Durum wheat flour contributed to the fermented and winey attributes of CSB. Carbohydrates with higher abundance in Canadian wheat flour was involved in sugar-amine reaction and glucose conversion, which enhanced the sweetness of CSB. In addition, fatty acids, organic acids, amino acids, and glucose were crucial metabolites which can further formed into various characteristic compounds such as hexanal, hexanol, 2,3-butanediol, acetoin, and 2,3-butanedione and thus contributed to the winey, fresh sweet, and green aroma properties. This study is conductive to better understand the evolution of the compounds that affect the quality and aroma of CSBs.

Exploring the Effects of Freeze-Dried Sourdoughs with Lactiplantibacillus pentosus 129 and Limosilactobacillus fermentum 139 on the Quality of Long-Fermentation Bread.

Sourdough production is a complex fermentation process. Natural sourdough fermentation without standardization causes great variability in microbial communities and derived products. Starter cultures have emerged as alternatives to natural fermentation processes, which could improve bakery quality and produce bioactive compounds. This study aimed to evaluate the impacts of freeze-drying on the production and viability of sourdoughs with Lactiplantibacillus pentosus 129 (Lp) and Limosilactobacillus fermentum 139 (Lf), as well as their effects on the quality of long-fermentation bread. These strains were selected based on their better performance considering acidification and exopolysaccharide production capacity. Sourdough with Lp and Lf were propagated until the 10th day, when physicochemical and microbiological parameters were determined. The produced sourdoughs were freeze-dried, and bread samples were produced. The freeze-drying process resulted in high survival rates and few impacts on the metabolic activity of Lp and Lf until 60 days of storage. Incorporating Lp and Lf improved the microbiological and physicochemical properties of sourdough and long-fermentation breads. Tested freeze-dried sourdoughs led to reduced bread aging (higher specific volume and decreased starch retrogradation) and increased digestibility. The results show the potential of the freeze-dried sourdoughs produced with Lp and Lf as innovative strategies for standardizing production protocols for the bakery industry, especially for producing long-term fermentation bread.

Use of Agave Angustifolia Haw Bagasse Pulp as An Additive Powder For Baking Flour

Objective: The main objective of this study was to determine the conditions under which the pulp of Agave Angustifolia Haw bagasse fibers should be processed to obtain a good quality bread in terms of texture, emulsion, and crumb formation. Theoretical Framework: Agro-industrial residues have become in recent years alternative raw materials for different production processes, however, they must present conditions that allow food fortification, as is the case of agave residues whose nutrient content has evidence in the literature. Method: The methodology used in this study is experimental and applied in stages, 10 kg of agave bagasse were collected and processed; different proportions of agave flour were established in bread recipes to test the ideal fermentation for baking. Results and Discussion: The results obtained revealed that the fine residues obtained from the process of solar drying and grounding of agave bagasse pulp increase high-water absorption capacity, therefore this property favors the hydration of bakery products. Implications of the research: It is concluded that these residues in a lower concentration combined with flour can be used for the elaboration of bakery products with nutritional value. Originality/value: This study contributes to reduce agro-industrial waste contamination by transforming them into functional food, which represents a contribution to food security programs and social impact.

Autochthonous lactic acid bacteria in gluten-free sourdoughs produce nutritional and technological improvements in quinoa and buckwheat breads

Gluten-free (GF) baked goods available in the market are often poor in nutrients, such as protein, dietary fibre, micronutrients and bioactive compounds, and have low technological quality. Therefore, it is important to develop GF products with nutrient-rich flours, like quinoa (Q) and buckwheat (BW). Sourdough (SD) technology generates desirable nutritional and technological effects to improve GF bread. The objective of this work was to evaluate the effect of four autochthonous strains of lactic acid bacteria used as sourdough starters on the nutritional and technological quality of GF breads made with non-conventional flours. Lactiplantibacillus plantarum T3, Limosilactobacillus fermentum T4, Pediococcus acidilactici 26 and Pediococcus pentosaceus 82 were isolated in Q and BW flours. Nutritional and technological properties of flours, SD and breads were evaluated. In Q SD and breads, there was a 90% increase of polyphenols, 36% of ferulic acid and 131% in antioxidant capacity with respect to control compared to BW. In Q BW SD and breads, there were higher amounts of free amino acids and lower phytic acid content compared to SD controls. The incorporation of SD increased the specific volume, reduced the initial firmness of the crumb and the rate of hardening, produced a darkening of the crust; the crumbs of the breads were spongier, with a greater fraction of air and quantity of alveoli. Breads with Q SD presented better technological quality than breads with BW SD. Lim. fermentum T4 and P. pentosaceus 82 produced SD and breads with better technological quality.

Characteristics of whole wheat steamed bread production by two-stage fermentation procedure with Jiaozi as starter

Given the beneficial role of biological acidification in improving the quality of cereal-based products, this study evaluated the applicability of Jiaozi as a starter for whole wheat dough fermentation and steamed bread quality improvement. During the first 12 h of dough fermentation, yeast and lactic acid bacteria (LAB) populations increased significantly, resulting in rapid carbohydrate consumption and acidification of the dough. The lactic/acetic acid molar ratio was approximately 3.3, which was within the recommended fermentation quotient (FQ). Compared with the unfermented dough and the dough fermented for 12 h, the dough fermented for 6 h had an improved protein network structure, and the steamed bread made from it also showed the best quality. At a low leavening temperature of 28 °C, satisfactory specific volume and height/diameter ratio were obtained. The dough with an inoculation amount of 50% of the fully fermented dough showed the highest CO2 production capacity. Compared with yeast, the whole wheat steamed bread produced by Jiaozi using the two-stage fermentation process showed higher specific volume, resistant starch (RS) content and protein digestibility. The results showed that the use of Jiaozi as a starter has great potential for application in improving the quality of whole wheat steamed bread.

Impact of melon seed oil cake with different particle sizes on bread quality

Melon seed oil cake (MSOC), as the secondary by-product from melon seed oil pressing process, has high potential nutritional value. The aim of this study was to assess the effect of incorporating MSOC as wheat flour substitute and its particle size on bread quality; three particle size fractions of MSOC (coarse, medium, and fine) and two substitution levels (3 and 6%, w/w) were employed. Functional properties and colour of different particle sizes of MSOC were assessed, and the physical properties of bread made with MSOC were explored and compared to control bread (100% wheat flour). Results showed that bread made with 3% MSOC had relatively satisfactory quality in terms of specific volume (2.64–2.86 mL/g), hardness (14.31–15.04 N) compared to the control bread (specific volume 2.79 mL/g and hardness 13.87 N). Bread made with fine particle size of MSOC (2.64 mL/g and 15.04 N at 3% substitution level; 2.44 mL/g and 16.03 N at 6% substitution level) had lower specific volume and higher hardness values than the bread made with medium (2.80 mL/g and 14.31 N at 3% substitution level; 2.50 mL/g and 15.50 N at 6% substitution level) and coarse (2.86 mL/g and 14.72 N at 3% substitution level; 2.52 mL/g and 15.12 N at 6% substitution level) particle sizes of MSOC. These results indicate that using 3% MSOC with larger particle size could be more suitable for making bread with relatively satisfactory quality. Overall, MSOC could be re-introduced into food chain as ingredient for bread production, which offers possibilities to develop novel sustainable foods. Future work will be conducted on sensory quality and consumer acceptance to provide a desirable quality of bread.Graphical