Grape Quality Research Articles

VvERF117 positively regulates grape cold tolerance through direct regulation of the antioxidative gene BAS1

Cold stress significantly threatens grape quality, yield, and geographical distribution. Although ethylene-responsive factors (ERFs) are recognized for their pivotal roles in cold stress, the regulatory mechanisms of many ERFs contributing to tolerance remain unclear. In this study, we identified the cold-responsive gene VvERF117 and elucidated its positive regulatory function in cold tolerance. VvERF117 exhibits transcriptional activity and localizes to the nucleus. VvERF117 overexpression improved cold tolerance in transgenic Arabidopsis, grape calli, and grape leaves, whereas VvERF117 silencing increased cold sensitivity in grape calli and leaves. Furthermore, VvERF117 overexpression remarkably upregulated the expression of several stress-related genes. Importantly, BAS1, encoding a 2-Cys peroxidase (POD), was confirmed as a direct target gene of VvERF117. Meanwhile, compared to the wild-type, POD activity and H2O2 content were remarkably increased and decreased in VvERF117-overexpressing grape calli and leaves, respectively. Conversely, VvERF117 silencing displayed the opposite trend in grape calli and leaves under cold stress. These findings indicate that VvERF117 plays a positive role in cold resistance by, at least in part, enhancing antioxidant capacity through regulating the POD-encoding gene VvBAS1, leading to effective mitigation of reactive oxygen species.

From Farm to Fork: Irrigation Management and Cold Storage Strategies for the Shelf Life of Seedless Sugrathirtyfive Table Grape Variety

Background: Sustainable water management for table grape has the primary goal of optimizing irrigation through Smart Irrigation (SI) approaches, particularly in Mediterranean regions. In addition, extending the shelf life of table grapes through effective cold storage practices is crucial to meet consumer demands year-round. This research examined the journey “from farm to fork” of Sugrathirtyfive variety (Autumn Crisp® brand), exploring the combined effects of Irrigation Volumes (IV), SO2-Generating Pads (SGPs) and Cold Storage Duration (CSD) on the quality of grapes. Methods: Normal Irrigation (NI—based on the farmer’s experience) and SI (100% vine evapotranspiration restored) were supplied in 2023 to Sugrathirtyfive variety white table grape, trained to an overhead tendone system. Yield and quality parameters, berry texture, CIELAB colour coordinates, phenolic content, flavonoids, antioxidant activity and sensory attributes were evaluated on grapes subjected to different times and methods of cold storage. Results: SI grapes showed higher Total Soluble Solids (TSSs) and nutraceutical content, as well as improved CIELAB coordinates with interesting improved berry texture parameters. No differences emerged between single- or dual-release SGPs after 15 days (T1) and 40 days (T2) of CSD. Conclusions: Under our cold storage conditions (3 °C, 85% U.R.), 40 days represent the maximum temporal limit for the cold storage of Sugrathirtyfive variety, regardless of IV, provided they are refrigerated with the aid of SGPs.

The Observed Changes in Climate Characteristics in the Trebinje Vineyard Area (Bosnia and Herzegovina)

The productivity and quality of grapes and wine are significantly influenced by changing climate conditions in vineyard regions worldwide. This study assesses changes in temperature, precipitation, and viticultural indices between the periods of 1971–1990 and 2000–2019 in Trebinje, a vineyard area located in the Herzegovina region of Bosnia and Herzegovina. Between the two periods, mean annual temperature increased by 2 °C and mean vegetational temperature by 2.4 °C, while mean precipitation remained within the range of climatological variability, with annual values increasing by 6% and vegetational values decreasing by 4.6%. Warming resulted in a longer duration of the vegetation season by 23.7 days, a reduced risk of late spring frosts, and an increased risk of very high temperatures during summer. These changes led to the reclassification of Trebinje vineyards’ climate from Region III to Region V, based on the Winkler index values, from a “temperate warm” to a “warm” category, based on the Huglin heliothermic index, and from “cool nights” to “temperate nights” based on the cool nights index. The category of the dryness index remained unchanged between the two periods. The findings emphasize the necessity for a renewal of the viticultural zoning and the development of climate change-adaptation plans for this region.

Overhead spray water treatment as a mitigation strategy to alleviate vine stress and safeguard grape quality during heatwaves

Changes in climate are influencing the quality of wine grapes worldwide. The impact of extreme climate events over short periods is increasingly recognised as a serious risk to grape quality and yield quantity. In this study, the mitigation effects of a pulsed water spray treatment on vine canopy during heatwave (HW) events were evaluated in relation to vine condition during the growing season and grape quality. In the UNCuyo experimental vineyard, vines of the three cultivars Malbec (ML), Bonarda (BO) and Syrah (SY) were treated with an overhead pulsed water spray. Heatwaves were defined as days with a minimum temperature of 21 °C and a maximum temperature of 35 °C. Two heat waves were identified during the growing season. Samples were collected at weekly intervals from veraison to harvest. On five sample dates, Leaf and Stem Water Potential (LWP, SWP), Stomatal Conductance (gs), Leaf Temperature (LT), Berry Temperature (BT), Chlorophyll Content (CC), Fluorescence (Fv/Fm) and Performance Index (PI) were recorded at several time points during the day to evaluate the physiological responses of the vine. Berries were collected on each sample date and at harvest. Berry weight, soluble solid content and pH were recorded. In the treated vines (Trt), LWP, SWP, Fv/Fm, PI and gs were significantly higher and LT was lower than in the control vines (Ctl) during the second heatwave, which was longer and more intense than the first one. One week after the more severe heatwave, LWP, SWP and gs remained significantly higher in Trt than in Ctl, displaying reduced physiological stress in Trt. At harvest, the anthocyanin profile, total polyphenol index (TPI), fruit yield, number of bunches and their average weight, berry weight, soluble solid content and pH were also recorded. Bunch weight was significantly higher in Trt for all cultivars. No differences were found in total anthocyanin concentration. These findings indicate that the vines subjected to targeted overhead water treatment during heatwaves underwent less physiological stress and yielded higher grape production, without increasing the risk of potential fungus diseases, in the Mendoza climate. Consequently, this practice could serve as a valuable strategy for mitigating the adverse effects of heatwaves.

Biocontrol and Enzymatic Activity of Non-Saccharomyces Wine Yeasts: Improvements in Winemaking

Wine fermentation is a biochemical process carried out by a microbial consortium already present in the vineyard, including different species of fungi and bacteria that are in an ecological relationship with each other, so that their sequential growth causes the transformation of grape must into wine. Among the fungi, the unicellular ones, yeasts, stand out, including Saccharomyces cerevisiae, which is mainly responsible for driving alcoholic fermentation, as do other species present from the beginning of fermentation, known as non-Saccharomyces yeasts. These yeasts were previously considered harmful and undesirable; however, their role has recently been re-evaluated, mainly because they can provide products and effects that are of great value in achieving a quality final product. In this review, we discuss the role of non-Saccharomyces wine yeasts, firstly with regard to their biocontrol activity both on the grapes and during the vinification process and secondly with regard to their ability to produce enzymes, especially depolymerising ones. In this context, the possible biotechnological applications of these non-Saccharomyces yeasts to improve the health and quality of grape and wine production are addressed.

Assessing the influence of climate controls on grapevine biophysical responses: a review of Ontario viticulture in a changing climate

Climate change presents unique challenges for grape growers across the world. In Ontario, three distinct viticultural regions are experiencing climatic shifts towards warmer growing seasons. According to historical records collected from Environment and Climate Change Canada, Lake Erie North Shore has transitioned from an intermediate-to-warm growing season classification, the Niagara Peninsula from the lower to upper limits of the intermediate zone, and Prince Edward County from cool to intermediate, when analyzing their average growing season temperatures. Terroir is directly related to vine water status, an indicator of grapevine stress. Biophysical responses controlled by air temperature and precipitation include fluctuations in vapour pressure deficits, evapotranspiration, and water-use-efficiency rates, as well as soil water content levels. By conducting an extensive literature review, the development of a conceptual model addresses how variations in climatic controls, under the scope of climate change, may influence grapevine water status, biophysical responses, and associated production outcomes for Ontario vineyards. Cool-to-intermediate air temperatures, when paired with increased precipitation will lead to no or low vine stress, increasing photosynthesis and transpiration rates, and variable plant water-use-efficiency levels, producing higher yields and lower quality grapes, if no management strategies are applied. Oppositely, higher air temperature as a product of climate change, when paired with variable precipitation may produce mild-to-severe stress, reducing yield, and increasing grape quality. With the appropriate management strategies, both traditional and new, growers may be able to accommodate for the influence of climate change on their vineyards.

Could varieties genetically related to Tempranillo behave better than it under drought conditions?

The effects of climate change are an emerging threat that is compromising the sustainability of grapevine growing worldwide. The Tempranillo variety is no stranger to this threat. In fact, in recent decades, significant losses have occurred in the yield and quality of Tempranillo grapes for winemaking. This problem is particularly serious in Spain, where Tempranillo is the red variety par excellence for the production of great wines. In this study, we assessed whether two genetically related varieties could exhibit a better drought response than Tempranillo itself. During two consecutive seasons, a study was conducted including the Tempranillo, Benedicto, and Moribel varieties, which were grown under two different water deficit regimes. The phenology of the varieties was followed, and components of yield, vigor, quality parameters, and δ13C were analyzed. The results revealed that Tempranillo was the variety most affected by water stress, exhibiting significantly higher δ13C than its relatives. Without compromising yield, Benedicto and Moribel responded better than Tempranillo to drought conditions, exhibiting better must quality even in the warmer season. The wines coming from Benedicto exhibited the highest quality because of their high total acidity, anthocyanin concentration, color intensity, and low pH. Although all three wines exhibited a similar profile, in the sensory analysis, tasters mostly preferred Benedicto and Moribel wines to Tempranillo ones.

Influence of methyl jasmonate and methyl jasmonate plus urea foliar applications on amino acids composition throughout ‘Tempranillo’ grape ripening over two seasons

This work studies the impact of foliar application of methyl jasmonate (MeJA) and methyl jasmonate plus urea (MeJA + Ur) on the evolution of amino acid content in ‘Tempranillo’ grapes during ripening, across two vintages. To achieve this goal, sample grapes were harvested at five different timing. Fol1: 1 day before first foliar application; Fol2: 1 day before second foliar application; Preharvest: 15 days after second foliar application; Harvest: the day of harvest; and Postharvest: 15 days after harvest. The effect of foliar treatments was season dependent, being effective to improve the amino acids content of grapes only in the first vintage. Among the treatments studied, foliar application of MeJA-Ur showed better results. The evolution of amino acids during ripening also was different among seasons. Overall, in the 2019, amino acids reached their highest content at Preharvest or Harvest samples, whereas in the 2020 season, these highest concentrations were reached at Postharvest. Asparagine might serve as a suitable amino acid for controlling grape ripening, as its content decreased from Fol1 to Postharvest in the two vintages. Moreover, differences on the total amino acids content at Harvest date between vintages were observed, probably due to different climatological conditions. Therefore, this study pioneers the examination of the impact of foliar applications of MeJA and MeJA + Ur on the amino acids evolution in ‘Tempranillo’ grapes during ripening. The need for further research is clear to comprehend the complex interaction between foliar treatments and grape amino acids dynamics for optimizing nitrogen quality of grapes.

Effects of pesticides on soil bacterial, fungal and protist communities, soil functions and grape quality in vineyards

Abstract Pesticides can have unintentional effects on non‐target organisms and change biotic communities. Such changes might be particularly important in soil microbial communities, which drive many ecosystem functions and may affect crop quality. Here, we investigated, in a three‐year study, how vegetation control (by herbicide application) and soil copper content (from long‐term copper‐based fungicide application) affect biodiversity and the community structure of soil bacteria, fungi and protists, and associated soil functions (respiration, decomposition) in Swiss vineyards. Furthermore, we determined the effects of these two management practices on grape quality as the most direct ecosystem service to farmers. Across all study years, the community composition of microorganisms was affected by herbicide application, however, a significant loss of operational taxonomic units (OTUs) was only observed in fungi and protists. Soil copper content reduced OTU richness of bacteria and protists in some years but had no significant effect on fungal richness. Copper changed the community composition in all three groups of soil microorganisms. While we found no effect of copper on soil functions, herbicide application reduced microbial respiration and biomass by about 39% and 45%, respectively. However, decomposition rates remained virtually unchanged by any pesticide. Yeast assimilable nitrogen (YAN) levels in grape were below the critical threshold of 140 mg/L in 40% of the vineyards without herbicide application and the variety Chasselas, whereas in vineyards with herbicide application, it was only 20%. Application of pesticides led to changes in richness and composition of soil microbial communities and directly reduced some soil functions (microbial biomass and respiration) but not all (decomposition). Some grape quality parameters can be indirectly enhanced by pesticide application, highlighting the trade‐off between the interests of nature conservation and the interests of the farmer. Balancing these two diverging interests requires the establishment of alternative vineyard management allowing reduced pesticide application.

Rain cover for table grapes and its benefits

Agriculture represents a vital component of the global economy, and table grape production is one of the most significant agricultural activities worldwide. However, farmers often face weather-related challenges, such as continues rainfall that can jeopardize the harvest and the quality of grapes. In this context, rain cover for table grapes has become an essential agricultural practice to protect from diseases the vineyards and the yield.

СОЗДАНИЕ ИНФОРМАЦИОННОЙ БАЗЫ ДАННЫХ ПО КАЧЕСТВЕННЫМ ПОКАЗАТЕЛЯМ ВИНОГРАДА НА ФОНЕ КОМПЛЕКСА АГРОКЛИМАТИЧЕСКИХ ПАРАМЕТРОВ И ИНДЕКСОВ, ХАРАКТЕРИЗУЮЩИХ ТЕПЛООБЕСПЕЧЕННОСТЬ ТЕРРИТОРИИ, ПРИМЕНЯЕМЫХ ДЛЯ ВЫДЕЛЕНИЯ ТЕРРУАРОВ КРЫМА

Climate is one of the main factors determining the possibility of grape cultivating and influencing its growth and development. Climatic factors have a huge impact on obtaining high-quality products of grape processing. In the process of work, in order to create an informational database, the archival data on the content of sugars and titratable acids in grapes from the vineyards of Crimea were collected and systematized. According to the criteria necessary for this study, we have selected the information on 8 varieties from the Steppe zone for 11 years, 1 variety from the Piedmont zone for 3 years, and 4 varieties from the South Coast zone for 6 years. By collecting and nonlinear interpolating of long-term observations from Crimean weather stations using geoinformation and mathematical modeling methods, the values of agroclimatic factors were calculated for each selected vineyard on the dates of determining the quality indicators of grapes during their ripening at the location points of the vineyards. For this purpose, 10 agroclimatic indicators characterizing the heat supply were selected. Based on the research results, basic principles for creating an informational database of agroecological resources and quality composition of grapes were developed. Informational database can be further modified and extended with various agroclimatic indicators. Subsequently, the accumulated array of database information will be used to identify patterns reflecting the influence of agroecological conditions on the formation of grape quality parameters for various types of production, which will serve as a basis for establishing viticultural and winemaking terroirs. Key words: CLIMATIC FACTORS, ZONES OF THE CRIMEAN PENINSULA, HEAT SUPPLY INDICATORS, GEOINFORMATION MODELING, MASS CONCENTRATION OF SUGARS, MASS CONCENTRATION OF TITRATABLE ACIDS

Differences in Soil Water Holding Capacity and Available Soil Water along Growing Cycle Can Explain Differences in Vigour, Yield, and Quality of Must and Wine in the DOCa Rioja

Soil water availability during the vine growth cycle can affect yield and grape quality. The objective was to evaluate the effect of soil water holding capacity (AWC) and available soil water (ASW) throughout the growing cycle on the nutritional status, vigor, production, and composition of grapes and wine. The study was conducted in the municipality of Uruñuela in the DOCa Rioja (Spain). The soils of four rainfed vineyard plots were characterized to determine AWC and its impacts on vine, grape, and wine composition. The N, P, and K foliar content, vigor, grape yield, berry weight, and composition of must and wine were analyzed in those vineyard plots during the period 2010–2014. The ASW was simulated in each plot and each year analyzed, considering the soil properties and the weather conditions, after model calibration in one plot in which soil water content was registered. The results showed that AWC influenced ASW along the growing cycle, so vines suffered from water stress in some periods of the vegetative cycle. Plots with higher AWC had higher ASW from fruit set to ripening and lower water stress during this period, which explains the higher N, P, and K foliar content, vigor and grape yield, and lower polyphenol and anthocyanin content in grapes and wines. The period where water availability had the most influence on the quality of the grapes was from veraison to ripening, during which ASW increased berry weight and acidity and decreased anthocyanins and polyphenolic compounds.

Effect of Adding Red Propolis to Edible Biodegradable Protein Films for Coating Grapes: Shelf Life and Sensory Analysis.

Red propolis is an active ingredient of great nutritional interest which offers numerous benefits as an antioxidant and antimicrobial agent. Thus, the objective of this research was to evaluate the application of an edible and antimicrobial gelatine coating containing red propolis to increase the shelf life of grapes. Gelatine films with an addition of 5, 10, 15, 20 and 25% of red propolis extract were produced to evaluate their antimicrobial activity using the disk diffusion test in solid media. The films with 25% red propolis extract showed antimicrobial activity against the bacteria Staphylococcus aureus and Pseudomonas aeruginosa. The grapes were coated with pure gelatine, without a plasticizer and with gelatine with 25% red propolis and then stored for 1, 4, 10, 19 and 25 days at temperatures of 25 °C and 5 °C. The results showed that the gelatine coating with propolis reduced the mass loss of grapes stored at 25 °C for 19 days by 7.82% and by 21.20% for those kept at 5 °C for 25 days. The pH, total titratable acidity, soluble solids and color of the grapes increased due to the ripening process. Furthermore, the sensory acceptability indexes of the refrigerated grapes with coatings were superior (>78%) to those of the control samples (38%), proving the effectiveness of the coatings in maintaining the quality of grapes during storage.

Effect of indoor dehydration on the chemical composition of Marselan grapes in Heyang of China.

Marselan is a red wine grape variety with great brewing prospects. The aim of this study was to investigate the effect of postharvest indoor dehydration on the quality of Marselan grapes. For two consecutive years, the harvested grapes were dehydrated naturally indoors (24-28°C). Fresh grapes were used as a control, and dehydrated samples were taken every 7 days during the period of dehydration until ending at day 28. Dehydration treatment increased degrees Brix, reducing sugars, glycerol, and malic acid. On day 7, there was an increase in protocatechuic acid, p-coumaric acid, and total tannin of 26.00%-27.73%, 11.43%-52.52%, and 39.74%-70.45%, respectively. With increasing dehydration time, total phenols, total flavonoids and total flavanols in the skins were decreased by 17.05%-38.13%, 24.32%-57.38%, and 17.05%-59.48%, respectively, with an increase in pH, citric acid, and ascorbic acid contents of grape juice by 7.66%-21.43%, 100%-137.50%, and 61.29%-258.82%, respectively. On day 21, the esters were increased by 1.10-1.75 factors. Partial least square-discriminant analysis result of volatile compounds showed that ethyl acetate, 1-propanol, 1-propanol, 2-methyl-, 1-hexanol, and 1-butanol, 3-methyl- were the predominant characteristic flavor compounds during dehydration of Marselan grapes. The effect of indoor dehydration on Marselan grape quality offered application value for China's later dehydration wine production.

Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells.

Proanthocyanidins (PAs) and anthocyanins are flavonoids that contribute to the quality and health benefits of grapes and wine. Salinity affects their biosynthesis, but the underlying mechanism is still unclear. We studied the effects of NaCl stress on PA and anthocyanin biosynthesis in grape suspension cells derived from berry skins of Vitis vinifera L. Cabernet Sauvignon using metabolite profiling and transcriptome analysis. We treated the cells with low (75 mM NaCl) and high (150 mM NaCl) salinity for 4 and 7 days. High salinity inhibited cell growth and enhanced PA and anthocyanin accumulation more than low salinity. The salinity-induced PAs and anthocyanins lacked C5'-hydroxylation modification, suggesting the biological significance of delphinidin- and epigallocatechin-derivatives in coping with stress. The genes up-regulated by salinity stress indicated that the anthocyanin pathway was more sensitive to salt concentration than the PA pathway, and WGCNA analysis revealed the coordination between flavonoid biosynthesis and cell wall metabolism under salinity stress. We identified transcription factors potentially involved in regulating NaCl dose- and time-dependent PA and anthocyanin accumulation, showing the dynamic remodeling of flavonoid regulation network under different salinity levels and durations. Our study provides new insights into regulator candidates for tailoring flavonoid composition and molecular indicators of salt stress in grape cells.

Effect of nitrogen and iron synergistic application on physiological growth, berry quality, and flavonoid content of wine grapes

Effect of nitrogen and iron synergistic application on physiological growth, berry quality, and flavonoid content of wine grapes

Improving open sun and indirect solar drying kinetics with physicochemical quality of regal seedless grapes by novel pretreatment solution

This paper reports a study of the physicochemical quality and the drying behavior of regal seedless grapes (RSG), pretreated with a novel solution (Olive oil: 2%, calcium carbonate: 2% and distilled water: 96%), then open sun-dried and dried in an indirect solar dryer. To show the effect of soaking time and temperature of the solution, two soaking times are carried out (120 and 240 s) at two temperatures (333.15 and 353.15 K). Quality indicat or such as shrinkage, color, rehydration capacity, titratable acidity, power of hydrogen (pH) and polyphenol content are evaluated. The results show that the time required to dry treated RSG varies from 220 to 328h during indirect solar drying (ISD) (during 428 h for untreated samples), and from 164 to 180 h during open sun drying (OSD) (during 290 h for untreated samples). The effective moisture diffusivity of solar-dried RSG ranges from 0.316 to 3.030 mm2s−1and that of open sun-dried regal seedless grape ranges from 0.706 to 5.700 mm2 s−1. Pretreatment affects the pH values significantly, whereas the rehydration capacity showes a large difference between the treated and untreated RSG. The solar-dried RSG has more stable color and ISD retains more of their polyphenol content.

Rapid Detection of Tannin Content in Wine Grapes Using Hyperspectral Technology.

Wine grape quality is influenced by the variety and growing environment, and the quality of the grapes has a significant impact on the quality of the wine. Tannins are a crucial indicator of wine grape quality, and, therefore, rapid and non-destructive methods for detecting tannin content are necessary. This study collected spectral data of Pinot Noir and Chardonnay using a geophysical spectrometer, with a focus on the 500-1800 nm spectrum. The spectra were preprocessed using Savitzky-Golay (SG), first-order differential (1D), standard normal transform (SNV), and their respective combinations. Characteristic bands were extracted through correlation analysis (PCC). Models such as partial least squares (PLS), support vector machine (SVM), random forest (RF), and one-dimensional neural network (1DCNN) were used to model tannin content. The study found that preprocessing the raw spectra improved the models' predictive capacity. The SVM-RF model was the most effective in predicting grape tannin content, with a test set R2 of 0.78, an RMSE of 0.31, and an RE of 10.71%. These results provide a theoretical basis for non-destructive testing of wine grape tannin content.

The load impact on the growth, productivity, quality of grapes and wines produced from the Alcor variety

The load impact on the growth, productivity, quality of grapes and wines produced from the Alcor variety

The effect of pre-harvest and post-harvest aloe vera gel treatments on fruit quality and storage performance of table grapes

This study investigated the impacts of pre-harvest, post-harvest, and combined pre-harvest+post-harvest Aloe vera gel treatments on various quality parameters of the ‘Red Globe’ grape cultivar during a 150-day storage period. The quality parameters assessed were weight loss, decay ratio, color attributes, firmness, soluble solids content (SSC), titratable acidity (TA), levels of total phenolics and total antioxidant capacity, as these are indicative of bioactive compounds. Post harvest Aloe vera treatment resulted in the lowest weight loss among three treatments, while the combined treatment surprisingly led to the highest weight loss. Pre-harvest treatments resulted in the lowest SSC values, while the combined treatments led to the highest values. Differences in TA value were not statistically significant, neither between treatments nor during the storage period. Storage duration alone significantly affected pH values. The Aloe Vera treatments significantly increased total phenols compared to the control group, and this increase remained consistent throughout the storage period. The combined treatments yielded the highest total phenols (3389.45 µg gallic acid equivalent/g fresh fruit). Similarly, Aloe Vera treatments significantly increased total antioxidant capacity (TAC) throughout the storage period. While the combined treatment yielded the highest TAC on day 150 (6.66 µMol trolox equivalent/g fresh weight), it negatively affected physical and chemical parameters. However, compared to other treatments, this combined approach significant boosted phytochemical levels. These findings suggest that for fresh table grapes, separate pre-harvest or post-harvest Aloe Vera treatments might be preferable. On the other hand, for those prioritizing elevated phytochemical content for health benefits, the combined application offers an option despite its drawbacks.