Quail Egg Yolk Research Articles

Comprehensive risk assessment of lead concentrations in chicken, quail, and duck egg albumen and yolk using Monte Carlo simulations

This study conducted a comparative analysis of the concentration of the lead (Pb) in the albumen and yolk of eggs from domesticated chicken, quail, and duck, with a concurrent assessment of the potential carcinogenic and non-carcinogenic risks associated with the consumption of eggs sourced from Türkiye. A total of 78 poultry egg samples were gathered from breeding farms and farmers' markets situated in Şanlıurfa province. Lead concentrations were assessed through inductively coupled plasma optical emission spectrometry (ICP-OES). Human health risk assessment adheres to the guidelines set forth by the United States Environmental Protection Agency (US EPA), which primarily emphasizes estimated daily intake (EDI), international lifetime cancer risk (ILCR), target hazard quotient (THQ), and Monte Carlo simulation (MCS) as a probabilistic approach. One-way analysis of variance (ANOVA) was employed to compare Pb concentrations within egg yolks and albumens, as well as among various types of eggs. The levels of Pb found in the albumen of chicken, quail, and duck eggs were measured to be 0.31 ± 0.11, 0.43 ± 0.11, and 0.47 ± 0.16 μg kg−1, respectively. The concentrations of Pb in the yolks of chicken, quail, and duck eggs were found to be 0.54 ± 0.19, 0.28 ± 0.11, and 0.69 ± 0.21 μg kg−1, respectively. These concentrations were below the maximum permitted levels set by the FAO/WHO. The results indicated that Pb content in all tested eggs was safe for consumption, with exposure levels significantly below Joint FAO/WHO Expert Committee on Food Additives (JECFA) risk thresholds. The THQ values were less than one, indicating no non-carcinogenic risk. In addition, this study provides accurate and reliable data for policy makers to improve food safety measures and reduce potential public health risks.

Comprehensive lipidomics and flavoromics analysis reveals the formation mechanism of the unique flavor in quail egg yolks during thermal treatment

After thermal treatment, quail eggs pruduce an attractive flavor that is favored by consumers. In this study, the key aroma volatiles and their lipid precursors during thermal treatment were investigated by the electronic nose, GC-MS, GC-O-MS and UPLC-Q-Exactive HF-X. The results exhibited that the raw and fresh flavor of raw egg yolks came from 1-Octen-3-ol, 2-Ethyl-1-hexanol, 1-Decene, and 1-Undecene. 2-Methyl-3-octanone, Toluene, and some aromatic compounds worked synergistically to contribute to the aroma profile of boiled eggs. (+)-2-Bornanone, Octanal, 2-Methyl-butanal, Nonanal, (.+Dihydro-3-hydroxy-4,4-dimethyl-2(3 H)-furanone, 6,7-Dihydro-2,5-dimethyl-5 H-cyclopentapyrazine, (E)-2-methyl-6-(1-propenyl)-pyrazine, 2-Ethyl-3,5-dimethyl-pyrazine, 3,5-Diethyl-2-methyl-pyrazine, 2,5-Dimethyl-pyrazine were the key flavor compounds in the fried egg and gave it the popcorn and roasted flavors. The statistical analysis of the lipid profile revealed that brief, high-temperature heating (100 or 200 °C for 10 min) in typical boiled and fried quail eggs had minor effects on the lipid nutritional value. PE-related lipids, particularly those containing 18-carbon fatty acids, contributed to the aroma formation of fried quail eggs.Graphical

Quantitative metabolomic analysis of yolk granules from different poultry eggs

A systematic comparison of the nutrient composition of yolk granules from different poultry egg sources may provide a reference for the development of nutritionally balanced foods with high protein, low fat, and low cholesterol content. The metabolomic profiles of chicken, duck, and quail egg yolk granules were analysed quantitatively using UPLC-MS/MS. A total of 820 metabolites were identified, with amino acids and their metabolites (232), glycerophospholipids (121), and organic acids and their derivatives (96), representing the three most abundant metabolite classes in egg yolk granules. The quantitative results demonstrated that amino acids and their metabolites, as well as glycerophospholipids (GP), exhibited significant differences among the three egg yolk granules. A total of 13 coenzymes and vitamins were identified in the three egg yolk granules, with the majority belonging to the B vitamins and their derivatives. A total of 11 bile acids were identified in the three egg yolk granules, including four primary and seven secondary bile acids. A total of 20 significantly different metabolites were preliminarily identified as potential markers for the differentiation of poultry egg yolk granules. This information provides crucial insights into the nutritional composition of egg yolk granules from diverse sources.

Antihypertensive Potential of Japanese Quail (Couturnix Couturnix Japonica) Egg Yolk Oil (QEYO) in Sprague Dawley Rats.

Oils from animal sources have been used for centuries in the management of diseases. This research was conducted to screen the ex vivo and in vivo toxicity of quail egg yolk oil (QEYO) extracts and assess their effects on the management of hypertension in rats. QEYO was extracted using gentle heating (GH) and n-hexane (NHN). The extracts were subjected to toxicity testing using the hen's egg test on chorioallantoic membrane (HET-CAM) and bovine corneal histology test. Acute and sub-chronic toxicity (28 days) were evaluated in rats. Hypertension was induced in rats by administering 80 mg/kg of Nω-L-Arginine Methyl Ester (L-NAME) per day for 28 days. Treatments commenced on the 14th day; Nifedipine at 30 mg/kg and 1 mL of distilled water were administered as positive and negative controls. Blood pressure (BP), lipid profiles, and oxidative stress markers were quantified. No irritation was observed using the HET-CAM test in the egg treated with both extracts. Bovine corneal histology showed no lesions in all treated groups. No signs of toxicity were observed in either acute or sub-chronic toxicity studies. A significant reduction in blood pressure was observed in rats treated with the extracts (p < 0.05). Changes in total cholesterol (TC), triglycerides (TGs), low-density lipoproteins (LDLPs), and high-density lipoproteins (HDLPs) were not significant compared to the control (p > 0.05). Oxidative stress markers (SOD and CAT) increased significantly in the treated groups compared to the control, while the malondialdehyde levels decreased (p < 0.05). QEYO was safe in both ex vivo and in vivo studies and can be said to have the potential to lower blood pressure as well as cardio-protective effects in hypertensive rats. This research provides evidence based on which QEYO could be used safely as an adjuvant therapy in eye drops and cosmetics and can be considered an effective choice for preventing hypertension.

Imunitas dan Produktivitas Puyuh Periode Bertelur dengan Pemberian Tepung Daun Senduduk (Melastoma malabathricum L.) dalam Pakan

Senduduk leaf flour (Melastoma malabatricum L.) is a processed product from weed plants that can potentially be a source of nutrients and bioactive compounds that are beneficial for human and livestock health. This research aims to examine the immunity and productivity of quail during the egg-laying period by administering senduduk leaf flour. This study used 120 female quail aged 40 weeks, which were kept for 4 weeks. The experimental design used was a Completely Randomized Design. The treatment of providing senduduk leaf flour in feed consisted of four treatment levels, namely P0 (without flour), P1 (feed + 1.5% flour), P2 (feed + 3% flour), and P3 (feed + 4.5% flour). The data were analyzed using Analysis of Variance and descriptive analysis. The results showed that adding senduduk leaf meal can reduce egg yolk cholesterol and increase quail immunity. Adding senduduk leaf meal at a 3% level produced the best immunity and performance, with a feed conversion value of 2.81. The 4.5% addition of senduduk leaf flour produced the lowest cholesterol level in quail egg yolk. Keywords: egg quality, immunity, performance, quail, senduduk leaf

Antioxidant and Anti-Inflammatory Properties of Quail Yolk Oil via Upregulation of Superoxide Dismutase 1 and Catalase Genes and Downregulation of EIGER and Unpaired 2 Genes in a D. melanogaster Model.

Quail egg yolk oil (QEYO) has a rich history of medicinal use, showcasing heightened antioxidant and bioactive properties in our prior studies. This positions QEYO as a promising candidate for therapeutic and cosmetic applications. In this investigation, QEYO was extracted using ethanol/chloroform and 2-propanol/hexane solvents. GC-MS and FTIR analyses quantified 14 major bioactive compounds in the ethanol/chloroform fraction and 12 in the 2-propanol/hexane fraction. Toxicity evaluations in fruit flies, spanning acute, sub chronic, and chronic exposures, revealed no adverse effects. Negative geotaxis assays assessed locomotor activity, while biochemical assays using fly hemolymph gauged antioxidant responses. Real-time PCR revealed the relative expression levels of the antioxidant and anti-inflammatory genes. FTIR spectra indicated diverse functional groups, and the GC-MS results associated bioactive compounds with the regulation of the anti-inflammatory genes EIGER and UPD2. While no significant change in SOD activities was noted, male flies treated with specific QEYO doses exhibited increased catalase activity and total antioxidant capacity, coupled with a significant decrease in their malondialdehyde levels. This study offers valuable insights into the bioactive compounds of QEYO and their potential regulatory roles in gene expression.

PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN TUMBUHAN AKAR KAIK-KAIK (Uncaria cordata (Lour.)Merr) TERHADAP KADAR TRIGLISERIDA SERUM TIKUS PUTIH (Rattus norvegicus) JANTAN

Hypertriglyceridemia is a condition when there is an increase in triglyceride levels in the blood. Hypertriglyceridemia can cause heart problems. One of the plants that can be used as medicine is the root plant kaik-kaik (Uncaria cordata (lour.) Merr). This study aims to determine the effect of giving ethanol extract of kaik-kaik (Uncaria cordata (lour.) Merr) leaves on serum triglyceride levels of male white rats (Rattus norvegicus). The parameters used were serum triglyceride levels of male white rats induced by high fat feed, namely quail egg yolk as much as 10 ml/kgBB. This study used 18 male white rats which were divided into 6 groups with 3 rats per group. The normal group was fed only with rats and Na CMC suspension, the negative group was given high-fat feed induction and Na CMC suspension. The positive group was given high-fat feed induction and 0.9 mg/kgBB of atorvastatin suspension. The treatment group was given high-fat feed and a suspension of ethanol extract of kaik-kaik root leaves at doses of 100, 200, and 400 mg/kgBB as much as 1% of body weight given orally. The method used was colorimetric enzymatic using a Mindray® photometer (BA-88A) at a wavelength of 510 nm. Triglyceride level data on days 0, 15, 22 and 29 were analyzed by one-way ANOVA test and repeated ANOVA. The results showed that the ethanol extract of kaik-kaik root (Uncaria cordata (Lour.) Merr) leaves at doses of 100, 200 and 400 mg/kgBB had an effect in reducing serum triglyceride levels of male white rats (Rattus norvegicus) indicated by differences. which was significant (p &lt;0.05) with negative control and showed activity to decrease triglyceride levels which was not significantly different (p&gt; 0.05) with atorvastatin.

Review Jurnal: Efektivitas Penggunaan Kuning Telur Berbagai Jenis Unggas Sebagai Pengencer Semen pada Ternak

A method is needed that can be used to maintain sperm quality in order to produce superior seeds. The use of poultry egg yolk as a diluent is of course widely used because poultry eggs can maintain the good quality of cattle sperm. This research aims to determine the level of effectiveness of egg yolks of various types of poultry as a sperm diluent on the motility and viability of cattle sperm. The method used in this research is the literature review method and the descriptive method. The results showed that the use of free-range chicken egg yolk and quail egg yolk as sperm diluent showed the highest motility values. The highest percentage of viability was in purebred chickens, while the lowest abnormality was in dilution with goose yolk eggs.

Clinical Features of Quail Egg Ingestion in Patients with Acquired Tolerance to Hen Eggs: A Case Series Study

Introduction: Patients with hen’s egg allergy are often instructed to avoid consuming other avian eggs, such as quail eggs. However, it is unclear whether patients with an acquired tolerance to hen eggs continue to avoid consuming quail eggs. This study aimed to evaluate the clinical features of quail egg ingestion. Methods: This prospective case series included children aged ≥1 year with hen’s egg allergy who were recruited between October 2019 and February 2021 in our hospital. We conducted an oral food challenge (OFC) with three boiled quail eggs to evaluate the clinical features of quail egg ingestion in patients with acquired tolerance to hen eggs. The primary outcome was a positive OFC after ingesting three quail eggs. Secondary outcomes were cross-antigenicity between hen and quail eggs observed through the skin prick test (SPT) and pattern of quail egg allergy, comprising the onset of reaction, and severity. The correlation between the diameters of the wheals with SPT in hen and quail eggs was evaluated using the Pearson product-moment correlation coefficient. Results: A total of 62 patients underwent the quail egg OFC. The median (interquartile range) age of the participants was 3 (2–5) years. Thirty-three (53%) patients had a history of anaphylaxis due to hen eggs. The median total immunoglobulin E (IgE) level in patients who underwent the OFC with half a heated whole hen’s egg was 271 (98–593) IU/mL. The median specific IgE level in egg white and ovomucoid was 9.7 (3.2–21.5) and 4.4 (1.3–6.9) UA/mL, respectively. The quail egg OFC results revealed that none of the 59 patients who ate the three quail eggs completely had an allergic reaction. The SPT-positive and SPT-negative rates in raw and boiled hen and quail egg whites were both correlated. The diameters of wheals with SPT in raw hen and quail egg whites and yolks were positively correlated. Conclusion: Patients with an acquired tolerance to hen eggs may not be required to avoid consuming quail eggs.

Efektivitas Ekstrak Daun Salam (Syzygium polyanthum) Dalam Mencegah Kenaikan Kadar Kolesterol LDL Tikus Putih (Rattus norvegicus) Diinduksi Kuning Telur Puyuh

Quail egg yolk contains high cholesterol which can trigger dyslipidemia. If Cholesterol is consumed in excess, will increase lipid profile, which can increase LDL cholesterol levels. Bay leaf extract contains several active compounds, like quercetin, a class of flavonoids with antioxidants that can prevent LDL oxidation. The aim of this study is to ensure the effectiveness of bay leaf extract on LDL cholesterol levels in white rats induced by quail egg yolk. The animal tested in this study were male white rats (Rattus norvegicus) with the Sprague Dawley strain. Samples were randomly selected and divided into five groups: three groups given quail egg yolk and bay leaf extract in various doses of 0.18 g/day, 0.36 g/day and 0.72 g/day, positive control given quail egg yolk, and negative control without treatment. The ANOVA test showed that there were significant results between the treatment groups, positive control and negative control (p&lt;0.05). Next, the BNJ test showed that giving bay leaf extract (0.18 g/day, 0.36 g/day) had a significant effect on LDL cholesterol levels (p&lt;0.05), while a dose of 0.72 g/day showed that not significant (p&gt;0.05), so we can conclude that the dose of 0.72 g/day was the most effective in preventing an increase in LDL cholesterol levels.

Lipid sources and emulsifiers in Japanese quail diets as modulators of performance, egg quality and yolk fatty acid profile

Abstract Using emulsifiers in poultry diets may increase digestibility of alternative lipid sources and enrich poultry egg yolks altering their fatty acid composition. This study aimed to evaluate performance, nutrient digestibility, egg quality, egg quality according to their storage period, and the fatty acid profile of Japanese quail egg yolk (Coturnix coturnix japônica). The design was completely randomized, in which 270 female quails were allocated in a 3x2 factorial diet arrangement: three lipid sources (soybean oil, poultry fat, and beef tallow), supplemented or not with an emulsifier, following two nutritional strategies – a diet formulated to meet the nutritional requirements proposed by INRA and a diet formulated with a reduction of 96 Kcal/kg of feed and added emulsifier. The effects of interactions between the lipid sources and the emulsifier were studied, their isolated effects when interactions were absent and the effect of storage time, and their interactions with the factors evaluated for egg quality variables. Feed intake was greater when beef tallow was added to the diets, while feed conversion was worse for birds fed diets with soybean oil and the emulsifier. Quails fed the diet with the emulsifier and soybean oil produced better quality eggs represented by the higher Haugh unit. Diets with beef tallow and poultry fat provided a higher percentage of palmitic and unsaturated fatty acids in the quail egg yolk. Alternative lipid sources such as beef tallow and poultry fat can be used as a substitute for soybean oil with added emulsifiers in diets for egg-laying quails without impairing performance and egg quality. The use of emulsifiers with alternative lipid sources to soybean oil can be considered a nutritional strategy in laying quail diets, but the reduction of energy in the diet must be adequate for the species.

MODELING OF MICE AS TEST ANIMALS FOR A PRECLINICAL STUDY OF HYPOLIPIDEMIC AGENTS

Animal models suitable for preclinical research are necessary for the discovery of hypolipidemic agents. Various publications have presented alternative dyslipidemia animal models, but identifying a feasible and stable method would serve as a solid reference for researchers. This investigation aimed to establish a sustained dyslipidemia induction that persists after several days of intervention with a hypolipidemic agent. Six groups of mice, each consisting of five primary test animals and one reserve test animal, were used. After a seven-day acclimatization period, we induced each group for 14 days using three different methods: (1) 5% body weight of quail egg yolks (5% QEY), (2) 10% body weight of used cooking oil (10% UCOs), and (3) a combination of 5% QEY and 10% UCOs. Once all mice reached their peak lipid levels, we evaluated lipid performance through a seven-day intervention with simvastatin (0.026 mg/20-gram body weight) in one of the paired groups. A 14-day combined induction of 5% QEY and 10% UCOs resulted in a 39% elevation in mouse lipids compared to baseline levels. Our findings offer an alternative to traditional dyslipidemia models. However, the development of an animal model for dyslipidemia still poses challenges. Therefore, the identification of novel biomarkers capable of targeting dyslipidemia in humans is crucial.

Correlation between fatty acids levels in chicken, duck, goose, pigeon, quail and turkey egg yolks and post-thawed quality of ram semen.

The comparison between adding egg yolks (EY) of chicken, duck, goose, pigeon, Japanese quail or turkey to the Tris glycerol extender on the quality of ram semen before freezing and post-thawing was evaluated. The correlation between fatty acids levels in egg yolks of different avian species and the post-thawed quality of ram semen was studied. The pooled ejaculates collected from five rams were extended with Tris EY glycerol extenders containing the EY of chicken, duck, goose, pigeon, quail or turkey and cryopreserved at -196°C. The straws were evaluated before freezing and post-thawing for sperm motility using a sperm cell analyser, vitality using a FluoVit kit and abnormality using a SpermBlue stain besides plasma-membrane and DNA integrities using a hypo-osmotic swelling test and a Halomax kit, respectively. The moisture, ash, protein and fatty acid (FA) contents of EY of chicken, duck, goose, pigeon, quail and turkey were analysed using a gas chromatograph. The chicken and quail EY extenders significantly improved the total progressive motility (32.05 ± 1.41 and 31.68 ± 1.43, respectively), vitality, plasma membrane and DNA integrities and abnormalities of post-thawing ram semen in comparison with other EY extenders. Pigeon EY had the lowest saturated fatty acids (SFAs) in comparison with the other types of EYs. The chicken and turkey EYs had the lowest percentage of (monounsaturated fatty acids) MUFAs in comparison with the other types of EYs. The highest percentage of polyunsaturated fatty acids (PUFAs) was observed in the turkey, pigeon and chicken EYs which were considered double or triple their percentage in duck and goose EYs, respectively. Significant positive correlations existed between SFAs levels and total motility, vitality, plasma membrane functionality and DNA integrity (0.77, 0.80, 0.67, 0.52, respectively). Significant negative correlations existed between gondoic EY levels and total motility, vitality, plasma membrane functionality and DNA integrity. In conclusion, the EYs of duck, goose, pigeon or turkey cannot substitute the chicken EY in ram semen extenders as they gave lower post-thawing quality. The quail EY can be used as a good replacer for chicken EY in the extender used for cryopreservation of ram semen. The EY composition of FAs can significantly affect the quality of ram semen post-thawing.

Motility of rabbit buck semen extended with quail-egg yolk and turkey egg yolk citrate as rabbit buck semen extenders at refrigerated temperature

Motility of rabbit buck semen extended with quail-egg yolk and turkey egg yolk citrate as rabbit buck semen extenders at refrigerated temperature

UJI AKTIVITAS ANTIKOLESTEROL EKSTRAK BATANG Uncaria cordata (Lour.) Merr. TERHADAP KADAR KOLESTEROL TOTAL MENCIT JANTAN (Mus musculus)

The aim of this study was to determine the anticholesterol activity of the stem extract of Uncaria cordata (Lour.) Merr. on total cholesterol levels in male mice (Mus musculus). Before carrying out the anticholesterol activity test, phytochemical tests were carried out to determine the content of secondary metabolites in Uncaria cordata stems. Furthermore, the anticholesterol activity test was carried out in vivo using male mice, male mice were induced by high-fat diet (PTL) in the form of beef brain, quail egg yolk and propylthiouracil to increase cholesterol levels in mice until they experienced hypercholesterolemia, then mice were induced by plant extracts to see anticholesterol activity. Cholesterol levels were measured using a glucometer (Autocheck). Phytochemical test results showed the content of secondary metabolites in the form of flavonoids, saponins, alkaloids, tannins and terpenoids. The results of the anti-cholesterol activity test showed that there was anti-cholesterol activity as indicated by a decrease in cholesterol levels in mice with hypercholesterolemia. A decrease of 11.45% at a dose of 5.3 mg/30grW; 12.89% at a dose of 10.2mg/30grW and 21.05% at a dose of 21.2mg/30grW.&#x0D; Key words : Anticholesterol, Uncaria cordata (Lour.) Merr., Hypercholesterolemia

Profil Asam Lemak kuning Telur Puyuh Jepang yang Disuplementasi Jus Daun Afrika (Vernonia amygdalina Del) dalam Air Minum

This study aimed to determine the effect of african leaf juice (Vernonia amygdalina) supplementation in drinking water on the performance and fatty acid profile of quail egg yolk. A total of 120 eight-week-old Japanese quail was used in this study and reared for 6 weeks in colony cage. Completely randomized design with three treatments and five replications were used in this study. The treatments were control treatment (without african leaf juice supplementation), 3 ml bird-1 day-1 supplementation and 6 ml bird-1 day-1 supplementation. The data were analyzed for variance, the significant difference results were tested by LSD test at p&lt;0.05. The variables were feed consumption, drinking water consumption, egg production, egg weight, egg mass, feed conversion and mortality as well as analysis of egg yolk fatty acid. The results showed that african leaf juice contained high flavonoid and saponin. Daily supplementation of african leaf juice in drinking water at the doses 3 and 6 ml bird-1 day-1 did not affect the performance of laying quails. Mortality in treated quail was 33.3% and this value was lower than control. Ratio of omega 6 and omega 3 was ranging 24.5% – 26.2% fat. It could be concluded that african leaf juice could use to reduced mortality.

Effect of freeze-dried quail egg white and yolk addition tosemen extender on viability of rooster sperm stored for 6hat 4°C.

The effect of freeze-dried quail egg white and yolk addition to basic EK extender on morphology and motility of chicken broiler breeder semen was investigated. Fresh pooled semen was divided into eight parts: fresh, undiluted (control), diluted in 1:2 ratio (v/v) with basic EK extender, EK + 200 mg/ml of egg white, EK + 100 mg/ml of egg white, EK + 50 mg/ml of egg white, EK + 100 mg/ml of egg yolk, EK + 50 mg/ml of egg yolk, EK + 25 mg/ml of egg yolk. Semen samples were evaluated 15min after dilution and after 6h of storage at 4°C. In the fresh semen, the number of live normal sperm was the highest in semen diluted with EK + 200 mg of egg white and EK + 100 mg of egg yolk, while the highest sperm motility was in the neat semen. Semen storage reduced the number of normal sperm in all analysed semen samples. In the neat semen, the number of normal sperm decreased, in relation to the fresh not-stored samples, by 36.8% (from 72.3% to 35.5%), with EK extender by 9.2%, in samples enriched with egg white, from 8.4% (EK + 200 mg) to 10.0% (EK + 100 mg), and in EK with egg yolk addition, from 1.2% (EK + 50 mg) to 10.6% (EK + 100 mg). The highest percentage of motile sperm was observed in EK extender enriched with 50 mg of egg white (77.1%) and EK + 25 mg of egg yolk (65.3%).

МОРФОЛОГИЧЕСКИЙ И БИОХИМИЧЕСКИЙ СОСТАВ ЯИЦ ПЕРЕПЕЛОВ, ДИВЕРГЕНТНЫХ ПО ЯЙЦЕНОСКОСТИ

Egg quality maintaining with high egg production is actual problem. This research task has been to analyze quail egg morphologic and biochemical composition in Omskaya quail breed in groups that are divergent in egg production (±1.5 ÷2σ from average). Eggs of quails with high egg production intensity had greater egg shell mass (by 0.19 g, P&lt;0.001) and less white mass (by 0.47 g, P&lt;0.05). Outer layers proportion has changed with egg production increasing. Protein content in quail egg white and yolk have been 10.64 and 16.71% in quails with high egg production that is less than in quails with low egg production by 0.1 and 0.23% respectively.

Pengaruh Ekstrak Etanol Daun Rhizophora apiculata terhadap Kolesterol Total dan Trigliserida Rattus norvegicus Galur Sprague dawley yang Diinduksi Diet Tinggi Lemak

Dyslipidemia is a lipid metabolism disorder in the form of an increase and decrease in the lipid fraction which is one of the high-risk factors for coronary heart disease and other non-communicable diseases. Rhizophora apiculata contains secondary metabolites in the form of flavonoid, tannin, alkaloid, saponin, and terpenoid which are thought to have the effect of preventing an increase in total cholesterol and triglyceride. This research is an experimental study with a posttest-only control group design carried out for 30 days using 30 experimental animals of Rattus norvegicus Sprague Dawley. There were 6 treatment groups, KN which was only given standard feed, K+ which was given quail egg yolk induction, simvastatin 10 mg/day, and K- which was given quail egg yolk induction. On the other hand, P1, P2, and P3 were given quail egg yolk induction, respectively. and leaf extract of Rhizophora apiculata with doses of 56mg/KgBW,m28mg/KgBW, and 14mg/KgBW. Then the rats were terminated using ketamine xylazine and blood was taken through the heart to be examined for total cholesterol and triglyceride using spectrophotometry. The results of the normality Shapiro-Wilk and Levene test p-value>0.05. In the One-way Anova test, cholesterol levels were 0.006 and triglyceride 0,002 (p-value<0.05), which indicated that there were significant differences between groups. The results of the post hoc LSD test on total cholesterol K- were significantly different from P1 and P2 and triglyceride K- were significantly different from P1, P2, and P3 (p-value<0.05). The administration of Rhizophora apiculata leaf extract had the effect of preventing the increase in total cholesterol and triglyceride levels of rats fed a high-fat diet.

The Effect of Avocado Drink (Persea Americana Mill) on the Incidence of Ballooning Hepatocyte in the Liver Organs of Rats (Rattus Novergicus) Induced by Hypercholesterolemia

BackgroundHypercholesterolemia is one of the risk factors for Non Alcoholic Fatty Liver Disease (NAFLD) which is characterized by the formation of ballooning hepatocytes. Avocados are one of the fruits that are easily available and contain high antioxidants and various active compounds. ObjectivesThe purpose of this study was to determine the effect of avocado drink on the incidence of ballooning hepatocyte in the liver of rats induced by hypercholesterolemia. MethodThis study used post test only with a control group design. The study used biological materials stored in rat liver organs which were divided into 5 groups (K1, K2, P1, P2, P3). Four groups (K1, P1, P2, P3) were induced by hypercholesterol diet with 5 ml/day quail egg yolk for 30 days, while the K2 group was given only BR-II rat feed. Groups P1, P2, P3 were given avocado drink as much as 2,3,4 ml/day for 30 days and then the rats were terminated to take their organs. Liver organs were stored in 10% formalin and made histopathological preparations stained with Hematoxylin Eosin (HE). Data analysis used the One-Way ANOVA test. ResultsBased on the One-Way ANOVA test, there was a significant difference (p<0.05) on the number of ballooning hepatocytes with a value of p=0.000. ConclusionThe effect of avocado drink (Persea americana Mill) had a significant effect on the incidence of ballooning hepatocyte in the liver organs of rats induced by hypercholesterol diet. Keywords: hypercholesterolemia, avocado drink, ballooning hepatocyte