The present study was performed to examine clinically and histopathologically the effects of maxillary expansion using the quad-helix appliance. Twenty seven young dogs were divided into nine groups of three dogs each. One group was used as control. A force of 600g was applied for expansion of the maxillary dental arch. Five groups were sacrificed at 1, 2, 3, 4, and 6 weeks after appliance insertion. Three other groups were sacrificed at 3, 4, and 6 weeks after appliance reactivation 2 weeks post-insertion. 1. At first the amount of maxillary arch width increased from 3 to 4 weeks after appliance insertion in the anterior area of the surviving dogs. But 5 weeks after insertion, the arch width of the posterior area was more expanded than that of the anterior area. 2. In comparison with the groups which were activated once, the amount of maxillary arch width was greater in the reactivated groups. 3. The quad-helix appliance caused wedge-shaped expansion of the midpalatal suture, with the anterior area more widely open as shown in the occlusal roentgenogram. 4. The histopathological examination of midpalatal suture was performed after maxillary expansion and tissue blocks were removed from the selected sites : the incisive area, maxillary area and palatine area. 1) The suture was separated but the sutural spaces were filled with newly formed bone, and the suture maintained its shape after maxillary expansion in all experimental groups. 2) In the reactivated groups, the new bone formation proceeded actively and the restoration of sutural area was satisfactory, although the amount of sutural expansion was greater than that of the singly activated groups. 3) In the incisive area, the histopathologic findings of both experimental groups indicated almost the same type of mechanical influences. 4) The maxillary area consisted of midpalatal, interincisive, incisivomaxillary and vomeroincisive sutures. The width of the sutural expansion was greater along the oral surface than along the nasal surface. In the singly activated groups, the expansion of each suture following new bone formation and restoration occurred very smoothly during all periods. But in the reactivated groups, in long range cases, the expansion of the midpalatal suture was greatest. Consequently bone formation in the maxillary area did not take place, in contrast with the singly activated groups. However, in the other sutures of the upper region, the restoration proceeded actively and the function and shape of the palatal vault were maintained by the compensating growth of the other sutures. 5) In the reactivated groups, the sutural expansion of the palatine area was slightly greater than in the singly activated groups.