You have accessJournal of UrologyKidney Cancer: Basic Research (IV)1 Apr 2013604 PREDICTIVE FACTORS OF IMPAIRED FROZEN KIDNEY TISSUE SAMPLES QUALITY IN THE SETTING OF ROUTINE SURGICAL ACTIVITY Grégoire Capon, Laurence Donon, Fabienne Soulet, Colette Deminiere, Jean-Philippe Merlio, Adelaïde Doussau, Marie Jullien De Pommerol, Guillaume Drutel, Alain Ravaud, Cathy Quemener, Grégoire Robert, Gilles Pasticier, Philippe Ballanger, Andréas Bikfalvi, Jean-Marie Ferrière, and Jean-Christophe Bernhard Grégoire CaponGrégoire Capon Bordeaux, France More articles by this author , Laurence DononLaurence Donon Bordeaux, France More articles by this author , Fabienne SouletFabienne Soulet Bordeaux, France More articles by this author , Colette DeminiereColette Deminiere Bordeaux, France More articles by this author , Jean-Philippe MerlioJean-Philippe Merlio Bordeaux, France More articles by this author , Adelaïde DoussauAdelaïde Doussau Bordeaux, France More articles by this author , Marie Jullien De PommerolMarie Jullien De Pommerol Bordeaux, France More articles by this author , Guillaume DrutelGuillaume Drutel Talence, France More articles by this author , Alain RavaudAlain Ravaud Bordeaux, France More articles by this author , Cathy QuemenerCathy Quemener Bordeaux, France More articles by this author , Grégoire RobertGrégoire Robert Bordeaux, France More articles by this author , Gilles PasticierGilles Pasticier Bordeaux, France More articles by this author , Philippe BallangerPhilippe Ballanger Bordeaux, France More articles by this author , Andréas BikfalviAndréas Bikfalvi Bordeaux, France More articles by this author , Jean-Marie FerrièreJean-Marie Ferrière Bordeaux, France More articles by this author , and Jean-Christophe BernhardJean-Christophe Bernhard Bordeaux, France More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.155AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES The aim of bio-banking is to allow further use of tissue samples in research. Surgeons and pathologists have to satisfy many precautions in order to ensure the samples quality. We wanted to assess the quality of our frozen kidney tissue bio-collection, so on validate our routine surgical procedures and find out predictive factors of sample degradation. METHODS Kidney cancer and matched normal kidney tissues were prospectively collected from 160 patients who underwent Surgery for Renal Cancer and immediately cryopreserved. RNA extraction was performed on 302 frozen samples using QIAGEN RNeasy mini Kit+©. Both quantitative (RNA concentration) and qualitative (RNA Quality Score - RQS) assessement was done. Clinical and histopathological data, the characteristics of surgical (surgical approach, operative time) and cryopreservation procedures were recorded for each patient and we looked for predictive factors of RNA impairment in univariate and multivariate analysis. RESULTS Tissue samples were collected from 116 Radical Nephrectomies (RN) (72%) and 44 Partial Nephrectomies (PN) (28%). The rate of laparoscopic procedures was 51%. Mean tumor size was 7,07 +/−6,95 cm. Median time to cryopreservation was 30 minutes (5-100). Mean RNA concentration for tumor and normal kidney samples were respectively 142,43 (±114,62) ng/μL and 115,06 (±114,62) ng/μL. Mean RQS value was 7,52 (±1,43) and 70% of the extracted RNAs scored higher than 7, that is generally considered suitable for further gene expression studies. Quantity and quality of paired samples were finally considered good enough in 133 patients (83%). In univariate analysis, high extraction efficiency was associated with year of surgery (p<0,001), general symptoms at diagnosis (p=0,012), sarcomatoïd component (p=0,011), necrosis (p=0,021) and high Fuhrman grade (p<0,001). Year of surgery (p<0,001) and Fuhrman grade (p=0,01) only, remained independent predictive factors of extraction efficiency. Healthy tissue and a pre-operative arterial embolisation were associated in univariate and multivariate analysis, with a significant RQS decrease : ΔRQS = 1.69 (p <0.001) and 1,44 (p<0,001), respectively. CONCLUSIONS This study validates our biobanking procedures in routine surgical practice with 83% of paired frozen samples suitable for molecular biology experiments. In multivariate analysis, Fuhrman grade and Year of surgery were associated with extraction efficiency; Tissue type (tumor or healthy tissue) and pre-operative arterial embolization significantly impacted RNA quality. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e247 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Grégoire Capon Bordeaux, France More articles by this author Laurence Donon Bordeaux, France More articles by this author Fabienne Soulet Bordeaux, France More articles by this author Colette Deminiere Bordeaux, France More articles by this author Jean-Philippe Merlio Bordeaux, France More articles by this author Adelaïde Doussau Bordeaux, France More articles by this author Marie Jullien De Pommerol Bordeaux, France More articles by this author Guillaume Drutel Talence, France More articles by this author Alain Ravaud Bordeaux, France More articles by this author Cathy Quemener Bordeaux, France More articles by this author Grégoire Robert Bordeaux, France More articles by this author Gilles Pasticier Bordeaux, France More articles by this author Philippe Ballanger Bordeaux, France More articles by this author Andréas Bikfalvi Bordeaux, France More articles by this author Jean-Marie Ferrière Bordeaux, France More articles by this author Jean-Christophe Bernhard Bordeaux, France More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...