QAMS Method Research Articles

Determination of five effective components in Sangju-Ganmao granules by quantitative analysis of multi-components by single marker

Objective To develop a method of quantitative analysis of multi-components by single marker (QAMS) to measure five effective components in Sangju-Ganmao grannules. Methods Withthe HPLC method, the chromatogram column was Agilent Zorbax SB-C18 (250 mm × 4.6 mm, 5 μm), the mobile phase was acetonitrile -0.2% physcion acid in gradient elution program, and the flow rate was 1.0 ml/min. The detection wavelength was set at 340 nm for chlorogenic acid and rutin, 205 nm for phillyrin, 254 nm for quercetin and glycyrrhizic acid, the column temperature was 30 ℃ and the injection volume was 10 μl. Using chlorogenic acid as the internal standard to measure the relative correction factor of four components like rutin, phillyrin, quercetin and glycyrrhizic acid by QAMS and calculate their content, and compare the cnents with the external standard method. Results The calculated values of chlorogenic acid, rutin, phillyrin, quercetin and glycyrrhizic acid were not statistical significant from those measured by external standard method (P>0.05). Conclusions The QAMS method can be used for the measurement of the content of five effective components in Sangju-Ganmao granules and the method is simple, effective and accurate. Key words: Chlorogenic acid; Rutin; Forsythin; Quercetin; Ammonium glycyrrhetate; Sangju-Ganmao granules; Quantitative analysis of multi-components by single marker; Quality control

Determination of seven ingredients of different grades Alismatis Rhizoma by QAMS method

The present study is to establish a quantitative analysis of multi-components by single marker(QAMS) for determining contents of seven compositions in Alismatis Rhizoma, alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B, alisol B 23-acetate and 11-deoxy-alisol B. Six relative correction factors(RCFs) of alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B and 11-deoxy-alisol B were established in the UPLC method with alisol B 23-acetate as the internal standard, which was to calculate the mass fraction of each. The mass fraction of seven effective constituents in Alismatis Rhizoma was calculated by the external standard method(ESM) at the same time. Compared with the content results determined by the ESM and QAMS, the feasibility and accuracy of QAMS method were verified. Within the linear range, the RCFs of alismoxide, alisol C 23-acetate, alisol A, alismol, alisol B, 11-deoxy-alisol B were 0.946, 4.183, 0.915, 1.039, 0.923 and 1.244, respectively, with good repeatability in different experimental conditions. There was no significant difference between the QAMS method and ESM method. Then, QAMS method was applied to determination of the different degree Alismatis Rhizoma from different areas. As a result, the concentrations of 7 components have differences in different areas, but no significant differences in different grades. The QAMS method is feasible and accurate for the determination of the seven chemical compositions, and which can be used for quality control of Alismatis Rhizoma.

Evaluation of anti-lipase activity and bioactive flavonoids in the Citri Reticulatae Pericarpium from different harvest time

BackgroundInhibition of pancreatic lipase is an attractive approach to the treatment of obesity and other metabolic disorders. Naturally occurring phytochemicals are promising sources of lipase inhibitors. PurposeIn the present study, the anti-lipase activity of Citri Reticulatae Pericarpium (CRP) extracts was firstly evaluated in vitro. Moreover, the dynamic alteration of bioactive flavonoids in CRP collected at different time and its correlation with anti-lipase activities was investigated. Study design/methodsQuantitative analysis of multi-components by a single-marker (QAMS) method was developed and validated for simultaneous determination of six flavonoids including narirutin, hesperidin, didymin, nobiletin, 3,5,6,7,8,3′,4′-heptamethoxyflavone and tangeretin. Anti-lipase activity evaluation and docking studies of the flavonoids was also carried out to screen out the candidate lipase inhibitors. ResultsThe QAMS method validation results exhibited that the developed method had desirable specificity, linearity, precision and accuracy. CRP collected in early months contained higher concentrations of bioactive flavonoids, and exhibited more potent anti-lipase activity. ConclusionHarvest timing had a significant impact on the amounts of bioactive flavonoids and the anti-lipase activities of CRP extracts. The contents of total flavonoids were positively correlated with the anti-lipase activities of CRP, and polymethoxyflavones played a significant role in the hypolipidemic effect of CRP. Nobiletin might be the most potential lipase inhibitor in CRP.