Pyrimidine Deoxynucleotides Research Articles

Deoxycytidine transport and pyrimidine deoxynucleotide metabolism in phytohaemagglutinin-stimulated pig lymphocytes.

Increased entry of deoxy[3H]cytidine begins at about 12h after addition of phytohaemagglutinin to peripheral pig lymphocyte cultures, and is accompanied by a parallel stimulation of deoxycytidine kinase up to the beginning of DNA synthesis at 24h. The increased deoxycytidine uptake is characterized by an increase in Vmax. without alteration of the apparent Km (0.7 +/- 0.11 muM). Although the entries of both nucleosides are promoted at the same time, the stimulation of deoxycytidine uptake is less than that of thymidine, and the two nucleosides are transported by separate systems. In addition to deoxycytidien kinase, the synthesis of deoxycytidylate deaminase and thymidylate synthetase are stimulated after addition of phytohaemagglutinin, but to a lesser extent than that of thymidine kinase. The importance of the latter enzyme in forming dTMP, and of thymidylate kinase in providing dTTP, is discussed.

Deoxyribonucleoside 5′-triphosphate pool fluctuations during the mamalian cell cycle

The levels of the four deoxyribonucleoside 5′-triphosphates have been measured during the cell cycle of selectively synchronised HeLa cells. All four pools show cyclic fluctuations. The pyrimidine deoxynucleotide pools increase sharply in size at about the same time as the onset of the S phase, while those of the purines increase slowly throughout the S phase. This difference in pattern may be correlated with the previously observed fluctuations during the cell cycle of thymidine and deoxycytidine kinases as against the constant levels of deoxyadenosine and deoxyguanosine kinases. It is concluded that pyrimidine deoxynucleotide biosynthesis is triggered at the same time as, or immediately prior to the initiation of DNA replication.

Enzymic biosynthesis of pyrimidine deoxynucleotides during [formula omitted][formula omitted] development

The in vitro activities of TMP and dCMP phosphatases, TMP and dCMP kinases and dCMP deaminase obtained from developing Rana pipiens eggs and embryos are presented. During embryogenesis only dCMP deaminase exhibits any marked changes in activity prior to embryonic hatching. The abrupt increase in dCMP deaminase which occurs following fertilization is due to dCTP activation of the enzyme.

Control of deoxynucleotide biosynthesis in Escherichia coli. I. Decrease of pyrimidine deoxynucleotide biosynthesis in vivo in the presence of deoxythymidylate.

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTControl of Deoxynucleotide Biosynthesis in Escherichia coli. I. Decrease of Pyrimidine Deoxynucleotide Biosynthesis in Vivo in the Presence of Deoxythymidylate*W. Dilworth Cannon Jr. and T. R. BreitmanCite this: Biochemistry 1967, 6, 3, 810–816Publication Date (Print):March 1, 1967Publication History Published online1 May 2002Published inissue 1 March 1967https://doi.org/10.1021/bi00855a022RIGHTS & PERMISSIONSArticle Views43Altmetric-Citations14LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (592 KB) Get e-Alerts Get e-Alerts

Simultaneous spectral determination of both base composition and concentration of DNA

This method provides a simultaneous spectral determination for both base composition and base concentration of DNA. Spectra of standard deoxynucleotide mixtures (adenylate with thymidylate and guanylate with cytidylate), hydrolyzed in 1 N acid, are directly proportional to spectra for DNA treated similarly. The significant products from hydrolysis are free purine bases and intact pyrimidine deoxynucleotides. There is a linear relation between the sum of the chosen differential absorbancies of the standard spectra and base composition. The base concentration of DNA is proportional to base concentration of the standards at an isosbestic point. The determinations are linear for base compositions of 0–100 mole % and for base concentrations of 0–100 μ M. Analyses of various DNA samples of animal and bacterial origin illustrate the practical application of the method. Literature values for base composition and phosphorus analyses for base concentration furnish comparisons with spectrally determined values as a test for accuracy. The method is not applicable to single-chain DNA's or to DNA's having unusual bases in high proportion.

HYDROLYSIS OF CALF THYMUS DEOXYRIBONUCLEATE BY PANCREATIC DEOXYRIBONUCLEASE: I. A STUDY OF THE OLIGONUCLEOTIDES LIBERATED IN THE PRESENCE OF MAGNESIUM OR MANGANESE IONS

The oligonucleotides obtained from deoxyribonucleic acid by the action of pancreatic deoxyribonuclease in the presence of magnesium ions or manganous ions have been analyzed by ion exchange chromatography and by determination of the relative amounts of purine and pyrimidine deoxynucleotides occupying the 5′-terminal position. Evidence of a difference in the specificity of action of the enzyme that is dependent upon the nature of the metal ion activator employed has been adduced.

HYDROLYSIS OF CALF THYMUS DEOXYRIBONUCLEATE BY PANCREATIC DEOXYRIBONUCLEASE: I. A STUDY OF THE OLIGONUCLEOTIDES LIBERATED IN THE PRESENCE OF MAGNESIUM OR MANGANESE IONS

The oligonucleotides obtained from deoxyribonucleic acid by the action of pancreatic deoxyribonuclease in the presence of magnesium ions or manganous ions have been analyzed by ion exchange chromatography and by determination of the relative amounts of purine and pyrimidine deoxynucleotides occupying the 5′-terminal position. Evidence of a difference in the specificity of action of the enzyme that is dependent upon the nature of the metal ion activator employed has been adduced.

The enzymatic deamination of 5′-deoxycytidylic acid and of 5-methyl-5′-deoxycytidylic acid in the developing sea urchin embryo

The importance of DNA synthesis during the early development of the sea urchin egg is discussed, as compared with the constancy of many other cellular constituents. The amount of enzymes which deaminate dCMP and MedCMP has been determined in different stages of development of the sea urchin embryo. A striking decrease in the two deaminases was observed, see Fig. 6. The following hypotheses have been discussed. (1) A cycle of conversion of the pyrimidine deoxynucleotides may exist in multiplying cells. In this way an appropriate assortment of pyrimidine deoxynucleotides is produced for DNA synthesis. (2) The deamination of dCMP to dUMP may have a pacemaker function for the DNA synthesis.