Compounds Pyrene Research Articles

Use of MQ 3 Sensor in Measuring Benzo[a]Pyrene Compound From Wood Husk Combustion Process with Robotdyn Uno R3 WiFi

Use of MQ 3 Sensor in Measuring Benzo[a]Pyrene Compound From Wood Husk Combustion Process with Robotdyn Uno R3 WiFi

Ultrasensitive Piezochromic Molecular Crystals: Mechanical Pressure‐Induced Controlled Regulation of TADF and RTP

AbstractConsidering that regulation of thermally activated delayed fluorescence (TADF) and room temperature phosphorescence (RTP) is necessary for a fluorophore to survive and properly show its characteristic features, it is successfully shown herein that a carefully designed novel pyrene derivative can exhibit remarkable aggregation‐induced emission (AIE) overcoming the characteristic fast excitonic decay of pyrene compounds. Moreover, it is established that J‐aggregation in the red‐emitting molecular crystals of the compound reduces the singlet‐triplet energy gap (∆EST), allowing TADF with a very high photoluminescence quantum yield (PLQY) of 72%. Whereas, anisotropic grinding of the same crystals generates well‐defined microcrystals that show RTP as the nature of aggregation changes. Impressively, under isotropic hydrostatic pressure, the crystals show near‐infra‐red (NIR) emission with a very high piezochromic luminescence sensitivity of 46.2 nm GPa−1. The results have established that formation of stable H‐aggregates in the microcrystals is responsible for the ultra‐long RTP. A highly sophisticated full‐spectrum Mueller‐matrix analysis is used for the first time in such systems to demonstrate the details of the effect of perturbation (pressure) on molecular conformation.

PAHs, Physicochemical and Microbiological Analyses of Trout Processed by Traditional Smoking, in Different Types of Packaging

Smoking represents one of the oldest thermic processing methods of fish, and it was originally used to preserve fish for later meals, delaying spoiling. The objective of this study was to analyze the physicochemical and microbiological safety and quality of two traditionally smoked trout species (Oncorhynchus mykiss and Salvelinus fontinalis). We analyzed the effect of trout processed by traditional smoking, characterizing the samples according to the relationships existing between classical microbial analysis, physicochemical parameters, and the content of polycyclic aromatic hydrocarbons (PAHs). The microbial activity of the smoked fish was very low. Although traditional smoking implies high temperatures and direct exposure to the smoke, Benzo[a]pyrene, Benzo[b]fluoranthene, Benzo[k]fluoranthene, Dibenzo[a,h]anthracene, Benzo[g,h,i]perylene, and Indeno [1,2,3-cd]pyrene compounds had undetectable levels in the samples. The products obtained in this study did not exceed the maximum values imposed by EU legislation regarding the TVB-N values during the 30 days of the experiment. We conclude that traditionally smoked trout has many advantages in terms of quality and safety of the products. Thus, the obtained products are safe for consumption for at least 30 days after processing.

Pyrene-Fused Poly-Aromatic Regioisomers: Synthesis, Columnar Mesomorphism, and Optical Properties.

π-Extended pyrene compounds possess remarkable luminescent and semiconducting properties and are being intensively investigated as electroluminescent materials for potential uses in organic light-emitting diodes, transistors, and solar cells. Here, the synthesis of two sets of pyrene-containing π-conjugated polyaromatic regioisomers, namely 2,3,10,11,14,15,20,21-octaalkyloxypentabenzo[a,c,m,o,rst]pentaphene (BBPn) and 2,3,6,7,13,14,17,18-octaalkyloxydibenzo[j,tuv]phenanthro [9,10-b]picene (DBPn), is reported. They were obtained using the Suzuki-Miyaura cross-coupling in tandem with Scholl oxidative cyclodehydrogenation reactions from the easily accessible precursors 1,8- and 1,6-dibromopyrene, respectively. Both sets of compounds, equipped with eight peripheral aliphatic chains, self-assemble into a single hexagonal columnar mesophase, with one short-chain BBPn homolog also exhibiting another columnar mesophase at a lower temperature, with a rectangular symmetry; BBPn isomers also possess wider mesophase ranges and higher mesophases' stability than their DBPn homologs. These polycyclic aromatic hydrocarbons all show a strong tendency of face-on orientation on the substrate and could be controlled to edge-on alignment through mechanical shearing of interest for their implementation in photoelectronic devices. In addition, both series BBPn and DBPn display green-yellow luminescence, with high fluorescence quantum yields, around 30%. In particular, BBPn exhibit a blue shift phenomenon in both absorption and emission with respect to their DBPn isomers. DFT results were in good agreement with the optical properties and with the stability ranges of the mesophases by confirming the higher divergence from the flatness of DBPn compared with BBPn. Based on these interesting properties, these isomers could be potentially applied not only in the field of fluorescent dyes but also in the field of organic photoelectric semiconductor materials as electron transport materials.

Two novel Anderson-type polyoxometalate based MnIII complexes constructed from pyrene derivatives: Synthesis, photophysical, and electrochemical properties

Symmetrical chloride-functionalized Anderson type POM afterwards used as a building block for onward POM post-functionalization with organic pyrene compounds and two novel organic–inorganic hybrid pyrene-POM compounds were designed, synthesized, and fully characterized. Photophysical and electrochemical properties of these compounds were investigated. Organic-inorganic hybrids retained the unique photophysical properties of their precursor pyrene compounds. Additionally, functionalization of inorganic POM moiety with organic bis-pyrene compound influenced the redox properties of the Mn-Anderson core.

Polycyclic aromatic hydrocarbons in suspended particulate matter of a region influenced by agricultural activities in northeast Brazil

Suspended particulate matter (SPM) samples were collected in rainy (2016) and dry (2017) seasons from the Japaratuba River in Northeast Brazil and analyzed the concentration of 16 priority polycyclic aromatic hydrocarbons (PAHs). The results were applied to measure the seasonal variations, spatial distributions, and source apportionment in the estuarine SPM systems. The seasonal variation in total PAHs was not statistically significant, although the highest levels were found in the rainy season, where the total PAHs concentrations in the Japaratuba River ranged from 71 to 1598 ng g−1, with a mean value of 453 ng g−1. In the dry season, the concentrations ranged from 65 to 459 ng g−1, with a mean value of 257 ng g−1. The composition profiles of PAHs at different sites were not significant. At most sampling sites, 5-ring and 6-ring compounds (indeno(1,2,3-cd)pyrene, dibenzo(a,h)anthracene, and benzo(g,h,i)perylene) were the most abundant components, followed by 2-ring and 3-ring compounds (naphthalene and phenanthrene). The use of diagnostic ratios (DR) and principal component analysis (PCA) showed that the PAHs mainly originated from pyrogenic sources. Ecological risk assessment indicated that the PAHs could cause moderate impacts on organisms, suggesting the need for preventive measures.

Organic compounds in surface and groundwaters in the surrounding of a Mexican geothermal reservoir; case study Los Humeros, Puebla

In this paper we evaluated if the Organic compounds (OC's) produced in the Los Humeros Geothermal Field (LHGF) could migrate to the surface and groundwaters in the surrounding areas; we report for the first time, the OC's of 2 maars, 24 springs, and 24 groundwater from sites surrounding the LHGF, in addition ten water/vapor condensates from the field were taken (all sampled in winter 2018). Using SPE-GC-MS, 141 OC's and 21 families were identified like alkanes, polyaromatics, sulfur-bearing, oxygen-bearing and halogen-bearing. Based on literature data, we assume that monoaromatics, alkanes and halogen-bearing could be some of the native families of the LHFG that migrate to the surrounding water bodies, without discarding an anthropogenic influence. In groundwater, 93 OC's and 19 families were identified. In the springs, 66 OC's grouped into 18 families were identified. In the case of the maars, 22 OC's and 10 families were identified. The most toxic polyaromatics identified in LHGF fluids are the heavy compounds pyrene and perylene which are naturally produced in the reservoir by combustion processes and carried to the surface by hydrothermal fluids at high temperatures. The results of this study do not prove a strong influence between the organic composition of condensates in the LHGF and the surrounding waters. How ever we provide new information to understand the dynamics of the LHGF. Furthermore, out of the scope of this study we could identify a strong anthropogenic influence on the sampling zone waters that can cause an environmental impact.

Synthesis of a dual-emissive pyrene-based fluorescent probe for imaging intracellular viscosity

A pyrene-based fluorescent molecular probe (Py2-5EG) was synthesized for optical detection of viscosity variation. Py2-5EG was synthesized by the reaction of two hydroxyl groups at the two ends of pentaethylene glycol with pyrene via a two-step reaction. Unlike typical pyrene compounds, the monomer fluorescence of Py2-5EG was not observed even in a dilute solution. Instead, the monomer fluorescence gradually increased with the increase in solvent viscosity. Blue fluorescent emission was observed from the monomer in a more viscous medium, and greenish blue emission from excimer was dominant in a less viscous medium in which dynamic excimer formation was involved. The observed fluorescence change of Py2-5EG was ratiometric and depended on the solution viscosity. A microscopic change in cellular viscosity was clearly observed inside and outside the mitochondria of living cells via different fluorescence colors of blue and green. The viscosity inside and outside mitochondria was determined using the fluorescence colors. Py2-5EG featured noticeable fluorescence emission shift in response to viscosity, and this phenomenon can be applied as a fluorescent probe, estimating the viscosity of various solutions as well as inside living cells.

Solution‐Spinning of a Collection of Micro‐ and Nanocarrier‐Functionalized Polysaccharide Fibers

AbstractContinuous polysaccharide fibers and nonwovens—based on cellulose, hydroxypropyl cellulose, chitosan, or alginate—containing biopolymeric microcapsules (MC) or mesoporous silica nanoparticles (MSN) are prepared using a wet‐spinning or solution blowing technique. The MCs are homogeneously distributed in the fiber matrices whereas the MSNs form discrete micron‐sized aggregates as demonstrated using scanning electron‐, fluorescence‐, and confocal microscopy. By encapsulating the model compound pyrene, it is shown that 95% of the substance remains in the fiber during the formation process as compared to only 7% for the nonencapsulated substance. The material comprising the MC has a strong impact on the release behavior of the encapsulated pyrene as investigated using methanol extraction. MCs based on poly(l‐lactic acid) prove to be practically impermeable with no pyrene released in contrast to MCs based on poly(lactic‐co‐glycolic acid) which allow for diffusion of pyrene through the MC and fiber as visualized using fluorescence microscopy.

Enhancing the interfacial strength of carbon fiber/epoxy composites by introducing “rigid-flexible” structure onto carbon fiber surface via π–π interaction

The "rigid-flexible" multi-scale gradient modulus interlayer was constructed by establishing the π-bridges between CF and CNTs with the assistance of pyrene compounds and chemically grafting polyetheramine (PEA) on the CF surface, and the effect of the gradient modulus interlayer on the interfacial properties of CF composites was studied. The construction of the "rigid-flexible" structure significantly increased the roughness and activity of CF surface, resulting in improving the mechanical interlocking and chemical bonding between fibers and the resin. The interfacial shear strength (IFSS), interlaminar shear strength (ILSS), transverse fiber bundle (TFB) strength and flexural strength of CF composites increased by 71.1%, 58.3%, 89.7% and 45.7%, respectively. The interfacial failure behaviors and strengthening mechanisms of the composites were also investigated. This scheme of establishing rigid-flexible gradient modulus interfacial layer with simple operation and nondestructive to fibers would provide a valuable reference for obtaining high performance CF composites.

Change in diagnostic ratios in expelled oils and residual extracts during semi-open pyrolysis experiments of an organic-rich shale

In order to investigate the effectiveness of diagnostic ratios in polycyclic aromatic hydrocarbon (PAH) source discrimination, semi-open pyrolysis experiments have been performed on an organic-rich, immature shale from the Winnipegosis Formation in southeastern Saskatchewan, Western Canada Sedimentary Basin. The concentrations and distributions of PAHs in expelled oils and residual extracts change drastically with increasing pyrolysis temperatures. The difficulty and inconsistency commonly encountered by using diagnostic ratios for PAH source identification in environmental samples seem to be rooted in the great variation of the diagnostic ratios themselves under different formation temperatures. No single diagnostic ratio allows a simple segregation of PAHs into petrogenic or pyrogenic sources. Some diagnostic ratios such as anthracene/phenanthrene and benz[a]anthracene/chrysene compound pairs are mostly effective for low-temperature pyrolysis, whereas indeno[1,2,3-cd]pyrene/benzo[ghi]perylene, aromatic hydrocarbon ring number distribution and degree of alkylation are mainly valid for high-temperature pyrolysis. The diagnostic ratios based on fluoranthene/pyrene, benzo[bk]fluoranthene/benz[a]pyrene compound pairs enjoy limited validity over a narrow pyrolysis range, whereas parameters derived from aromatic hydrocarbon ring number distribution, degree of alkylation and 1,7-/(2,6- + 1,7-dimentylphenanthrene) may be undistinguishable between petrogenesis and low-temperature pyrolysis. The apparent temperature-related variability must be taken into account when using the diagnostic ratios for source identification purposes. Multiple molecular markers need to be carefully selected to confirm the results obtained with PAH diagnostic ratios. Mechanical use of diagnostic ratios most likely leads to misinterpretation of environmental samples.

Effects of the absorbent types on changes in benzo[a]pyrene and volatile compounds in sesame oil

The effects of different absorbent types on changes in benzo[a]pyrene (BaP) and volatiles in sesame oil during filtration processes were investigated using gas chromatography–mass spectrometry analysis. The BaP content was greatly reduced after filtration using powdered activated carbons (activated carbon made from peat: PP, activated carbon made from coconut shell: PC, activated carbon made from wood: PW) in comparison to granular activated carbons (activated carbon made from coconut shell: GC, activated carbon made from wood: GW). The BaP content in sesame oil was also considerably reduced when filtrated with a PW–acid clay mixture in comparison to PW–kaolin and PW–celite mixtures. Most volatile compounds were also greatly reduced after filtration using powdered activated carbons (PP, PC, and PW) in comparison to granular activated carbons (GC and GW). These results might be due to the relatively ionic structure on the surface and internal surface area of absorbent pores.

Synthesis of pyrene-based materials with third-order nonlinearity optical property by click chemistry modification

PurposeCurrently, conjugated nonlinear optical (NLO) materials suffer from the drawbacks of complex manufacturing process and high cost. To further study the NLO materials with cost-effective, it is necessary to prepare new NLO materials with satisfactory performance.Design/methodology/approachPyrene derivatives with good third-order NLO properties were synthesized by combining pyrene compounds with TCNE, TCNQ, F4-TCNQ and other molecular systems by clicking chemical method.FindingsThe pyrene derivatives were characterized by ultraviolet spectrum and Z-scan. The charge-transfer of the D-p-A structures plays a key role in the absorption peak shifts. And the third-order nonlinear absorption of the products revealed good third-order NLO susceptibilities.Research limitations/implicationsThe synthesis technology of pyrene derivatives is not mature enough and is in the preliminary exploration stage. So, the authors produced a relatively small number of samples and did not conduct a very comprehensive test.Practical implicationsThis novel pyrene derivative is suggestive and can promote the exploration and development of the third-order nonlinear materials.Originality/valueFour new pyrene derivatives were synthesized by selecting new molecular systems. Because of its good chemical properties and stable properties, it can be a reference for the development of third-order nonlinear materials in the future.

Assessment on the lung injury of mice posed by airborne PM2.5 collected from developing area in China and associated molecular mechanisms by integrated analysis of mRNA-seq and miRNA-seq

Some epidemiological evidences showed exposure of airborne fine particulate matter (PM2.5) was associated with lung dysfunction. However, the adverse effects of PM2.5 from mid-scale city of China on the respiratory system were unknown. Correspondingly, the mechanisms, especially the epigenetic mechanism regulated by miRNAs, involved in PM2.5-induced lung injury has not been fully understood. In this study, male Balb/C mice were exposed to PM2.5 collected from mid-scale city (Baoji), China for 8 weeks (mean concentration 298.52 ± 25.86 μg/m3 at exposure chamber) using a whole-body exposure system. The carbon component was the main ingredient (45.80%) of PM2.5 followed by ions (43.19%). Meanwhile, the sum concentrations of polycyclic aromatic hydrocarbons (PAHs) and n-alkanes (C18-C33) were 570.48 and 2029.13 ng/m3 in the exposure chamber, respectively. Obvious lung injury including pulmonary inflammation and fibrosis (p < 0.05 compared with the control) were found from PM2.5 exposure group determined by micro-CT and histopathological assays, respectively, suggesting the health risk posed by PM2.5 from mid-scale city of China should be concerned. The integrated analysis between mRNA-seq and miRNA-seq revealed the differentially expression genes in lung tissues were enriched in immune pathways including B cell receptor signaling (p = 0.078) and cell adhesion molecules (CAMs) (p = 0.0068). The expression profiles of the genes and corresponding mRNAs involved into the immune pathways determined by RT-qPCR analysis were consistent with them conducted by transcriptome. Moreover, the expression levels of the proteins (i.e., CD19, CD81, PIK3CD, and CD22) involved into B cell receptor signaling pathway from exposure group were 1.71- to 6.948- folds compared with the control, validating the results of the genes expression profiles. Further, canonical correlation analysis (CCA) and multiple correlation analysis between the target genes and components of PM2.5 documented the organic compounds (i.e., Benzo(a)pyrene (p = 0.012) and octadecane (p = 0.05)) and inorganic elements (i.e., Cl-, Ti, Al, and Zn) was the key environmental factors. Cd19, Pik3cd, and Cd8b1 might be the key genes for lung dysfunction induced by PM2.5 illuminated using interactive analysis (p < 0.05). This work for the first time showed the adverse effects of PM2.5 in mid-scale city in China on respiratory system should be concerned, and the associated epigenetic mechanism regulated by miRNA were revealed. These results may provide new insight into the development of future assessment on the air pollution associated respiratory disease.

Chiral liquid crystalline networks demonstrating reversible optical texture and reversible fluorescence towards dynamic anti-counterfeiting

A dynamic surface pattern with tunable liquid crystalline texture and fluorescence in response to external stimulus can enable information recording and reading. Therefore, such patterns are widely used in the field of information security and anti-counterfeiting. A higher level of security in anti-counterfeiting is extremely desirable but challenging, herein, the chiral liquid crystalline networks (CLCN) used for anti-counterfeiting displaying both dynamic reversible Grandjean textures and fluorescence were dexterously designed and prepared by graft copolymerization of polymethylhydrosiloxane (PMHS), nematic liquid crystal M1, chiral crosslinker M2 and organic fluorescent pyrene compounds M3. The CLCN can emit blue-fluorescence when irradiating with ultraviolet light. More importantly, the Grandjean textures and fluorescence can be simultaneously and precisely adjusted by temperature. The as-prepared CLCN display reversible Grandjean textures during the heating and cooling process from the intrinsic chiral arrangement of CLCN. The discrete pyrene chromogenic segments in CLCN matrix enable the tunable photoluminescence by temperature, furthermore, the CLCN present highly sensitive photoluminescence evolution. The dynamic reversible Grandjean textures and fluorescence make the smart materials promising candidates in anticounterfeiting, providing a versatile strategy to fabricate intelligent optical anti-counterfeiting materials.

Changes in the indicators of chemical safety of drinking water in Ufa during its transportation to consumers

Introduction. To conduct a total assessment of carcinogenic, non-carcinogenic, and organoleptic risks to public health and cover both normalized and non-normalized pollutants of potable water, based on the results of long-term monitoring studies, it is possible using an integral indicator of chemical harmlessness of water. Material and methods. Authors studied potable water of water intakes of surface and infiltration types from potable water reservoirs and remote zones of Ufa water distribution networks. Impurities were determined by chromatography, inductively coupled plasma mass spectrometry, photometric, nephelometric, and titrimetric methods. Results. The main effect on the amount of carcinogenic risk in potable water of the surface water intake are chloroform, bromodichlethane, dichloroacetic acid, in the infiltration water intake - in addition to these compounds zinc, lead and chromium are in addition influenced. Organic compounds (phthalates, benz(a)pyrene, volatile aromatic compounds, etc.) have no effect on this type of risk due to the presence in water in low background concentrations. The identified number of individual carcinogenic risks for bromodichloroacetic acid and dichloroacetic acid according to the WHO classification corresponds to the second range. As the surface type water intake is removed from the potable water reservoir, the total carcinogenic risk of water decreases by 13-30%, and the infiltration type increases by 41-84%. Values of noncancerogenic and organoleptic risks are constant for potable water of different water intakes and correspond to recommended limit values. The excess of the maximum permissible concentrations (SanPin 1.2.3685-21) of individual substances in the potable water of the city has not been detected for the whole period of observation. In terms of the integral indicator of chemical harmlessness, the most favorable is the po-table water of the infiltration water intake, in the technology of which is mainly used the UV for decontamination of water. Conclusion. Using a risk assessment methodology complements the traditional approach to assessing water quality under modern hygiene standards. It becomes possible to evaluate the effect of pollutants of various classes on water quality when they are present together.

Fluorescent chiral liquid-crystalline networks with dual-mode temperature response

ABSTRACT Due to the excellent optical properties and great application prospects in the optical field, both pyrene compounds (PCs) and chiral liquid crystal have attracted extensive attention in theoretical research and practical applications. Fluorescent chiral liquid-crystalline networks (FCLCN) with good liquid-crystalline properties and tunable fluorescence properties as a response to temperature were prepared using Poly(methylhydrogeno)siloxane (PMHS), chiral liquid-crystalline monomer (M1), chiral crosslinker (M2) and organic fluorescent pyrene compounds (M3). The as-prepared FCLCN display reversible chiral Grandjean texture during the heating and cooling process from the intrinsic chiral arrangement of FCLCN. The discrete pyrene chromogenic segments in FCLCN matrix endowed the networks with excellent fluorescence properties when irradiating with ultraviolet light and the fluorescence can be precisely adjusted by temperature, furthermore, the FCLCN presents highly sensitive fluorescence evolution with a high response sensitivity of 3.4% K−1. The FCLCN can be manufactured into temperature-driven dynamic fluorescence patterns with a fast and reversible response.

Development of pyrene-based fluorescent ether lipid as inhibitor of SK3 ion channels.

We report the synthesis of three bioactive pyrene-based fluorescent analogues of Ohmline which is the most efficient and selective inhibitor of SK3 ion channel. The interaction of these Ohmline-pyrene (OP1-3) with liposomes of different composition reveals that only OP2 and OP3 are readily integrated into liposomes. Fluorescence measurements indicate that, depending on their concentration, OP2 and OP3 exist either as monomer or as a mixture of monomer and excimers within the liposome bilayer. Among the three Ohmline Pyrene compounds (OP1-3) only OP2 is able to reduce SK3 currents and is the first efficient fluorescent modulator of SK3 channel as revealed by patch clamp measurements (- 71.3±13.3% at 10μM) and by its inhibition of SK3-dependent cancer cell migration at (-32.5%±4.8% at 1μM). We also report the first fluorescence study on living breast cancer cells (MDA-MB-231) showing that OP2 is rapidly integrated in bio-membranes followed by cell internalization.

Effects of benzo[a]pyrene toxicity on morphology and ultrastructure of Hordeum sativum.

Many studies have been devoted to investigation of toxic benzo(a)pyrene (BaP) compound, but studies involving changes at the cellular level are insufficient to understand the mechanisms of polycyclic aromatic hydrocarbons (PAHs) effect on plants. To study the toxicity of BaP, a model vegetation experiment was conducted on cultivation of spring barley (Hordeum sativum distichum) on artificially polluted BaP soil at different concentrations. The article discusses the intake of BaP from the soil into the plant and its effect on the organismic and cellular levels of plant organization. The BaP content in the organs of spring barley was determined by the method of saponification. With an increase in the concentration of BaP in the soil, its content in plants also rises, which leads to inhibition of growth processes. The BaP content in the green part of Hordeum sativum increased from 0.3µgkg-1 in control soil up to 2.6µgkg-1 and 16.8µgkg-1 under 20 and 400ng/g BaP applying in soil, as well as in roots: 0.9µgkg-1, 7.7µgkg-1, 42.8µgkg-1, respectively. Using light and electron microscopy, changes in the tissues and cells of plants were found and it was established that accumulation of BaP in plant tissues caused varying degrees of ultrastructural damage depending on the concentration of pollutant. BaP had the greatest effect on the root, significant changes were found in it both at histological and cytological levels, while changes in the leaves were observed only at the cytological level. The results provide significant information about the mechanism of action of BaP on agricultural plants.

The Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Vaccine

Measles is an infectious disease that attacks children. incidence of measles in Indonesia is still quite high. results of the immunization implementation report stated that the achievement of immunization targets for children under five reached more than 90%. However, not all children in Indonesia can enjoy the benefits of the immunization that has been given. Humans in their lives cannot avoid benzo [a] pyrene compounds, either directly or indirectly. effect of benzo [a] pyrene on changes in immune cell profile in mice (Mus musculus L) was analyzed in vivo by giving intra-muscular injection of benzo [a] pyrene 20 mg / kg BW 2 times / week for 4 weeks. Immune cell profiles were analyzed using flowcytometry. analysis was continued with statistical analysis using SPSS software using the one way ANOVA test with a significance level (p-value <0.05). immune cell profile after week 4 in the group of mice that had received the vaccine and given exposure to benzo [a] pyrene was repression occurred in CD11b + IL1 +, CD11b + IL17 + activation occurred, while CD4 + CD25 + cell profiles experienced a downregulation reaction. How to Cite this Article Pubmed Style , Wijayanti LA, Rahayu DE. Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Vaccine. SRP. 2020; 11(9): 926-930. doi:10.31838/srp.2020.9.135 Web Style , Wijayanti LA, Rahayu DE. Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Vaccine. http://www.sysrevpharm.org/?mno=23317 [Access: March 30, 2021]. doi:10.31838/srp.2020.9.135 AMA (American Medical Association) Style , Wijayanti LA, Rahayu DE. Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Vaccine. SRP. 2020; 11(9): 926-930. doi:10.31838/srp.2020.9.135 Vancouver/ICMJE Style , Wijayanti LA, Rahayu DE. Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Vaccine. SRP. (2020), [cited March 30, 2021]; 11(9): 926-930. doi:10.31838/srp.2020.9.135 Harvard Style , Wijayanti, . L. A. & Rahayu, . D. E. (2020) Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Vaccine. SRP, 11 (9), 926-930. doi:10.31838/srp.2020.9.135 Turabian Style , Lumastari Ajeng Wijayanti, and Dwi Estuning Rahayu. 2020. Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Reviews in Pharmacy, 11 (9), 926-930. doi:10.31838/srp.2020.9.135 Chicago Style , Lumastari Ajeng Wijayanti, and Dwi Estuning Rahayu. The Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Vaccine. Systematic Reviews in Pharmacy 11 (2020), 926-930. doi:10.31838/srp.2020.9.135 MLA (The Modern Language Association) Style , Lumastari Ajeng Wijayanti, and Dwi Estuning Rahayu. The Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Vaccine. Systematic Reviews in Pharmacy 11.9 (2020), 926-930. Print. doi:10.31838/srp.2020.9.135 APA (American Psychological Association) Style , Wijayanti, . L. A. & Rahayu, . D. E. (2020) Effect of Benzo [A] Pyrene on Changes in the Profile of CD11B + IL1 +, CD11B + IL17 +, CD4 + CD25 + in Mice (MUS MUSCULUS, L) after Getting Paramicsovirus Reviews in Pharmacy, 11 (9), 926-930. doi:10.31838/srp.2020.9.135