The steel–concrete composite frame systems are widely used in multistory and high-rise buildings owing to good mechanical performance and high construction efficiency. In previous research on the composite frame systems, many lateral cyclic tests were conducted on specimens with small-width floor slabs without floor loads, which could not precisely reflect the actual behaviour of composite frames in spatial multistory buildings. Therefore, this paper presents an experimental study on the seismic behaviour of composite frames with wide floor slabs considering the effect of floor loads. The specimen was a two-story two-span composite frame with the scale ratio of 0.5, and three load cases, including the vertical floor loading test, the lateral cyclic loading test and the pushover test, were respectively applied to investigate the mechanical performance of the composite frame. The slab crack development, mid-span deflection and strain distribution of slab reinforcements in the vertical floor loading test were discussed. The overall responses of the specimen in the lateral cyclic loading test and pushover test were analysed, including failure phenomena, load–displacement curves, strength and stiffness degradation, energy dissipation capacity and deformation characteristics. Moreover, the seismic performance of columns, beams, slabs and joints in the specimen was discussed in detail and the force mechanism of the composite frame was revealed based on the structural analysis of components. The results indicated that the specimen exhibited sufficient load-bearing capacity, good ductility, stable strength and stiffness degradation and excellent energy dissipation capacity under the combination of vertical floor loads and lateral loads. The welds at the bottom flange near the exterior joint fractured under cyclic loading, which was found to have significant influence on the deformation pattern and force mechanism of the composite frame. The shear lag effect was obvious in the wide floor slabs and the effective width of the slabs was estimated to be nearly 0.75 times the column width.
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